FastQCFastQC Report
Fri 27 May 2016
SRR2139857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473107
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135300.050.6771131
GTATCAA241550.035.0487631
TCAACGC303550.027.3830264
ATCAACG304150.027.3562833
CAACGCA309750.026.8215335
TATCAAC316100.026.4284522
AACGCAG319150.026.1745116
ACGCAGA368400.022.574017
CGCAGAG374150.022.2160058
GTACATG339500.021.3202571
TACATGG343550.020.6342582
ACATGGG345500.020.1565693
GCAGAGT436850.018.809029
GAGTACT247400.018.28268212-13
CATGGGG243850.018.2001514
AGAGTAC403250.017.71735410-11
CAGAGTA432900.017.24641610-11
GTGGTAT61550.017.1849731
TGGTATC57600.016.9231872
ATGGGGG151300.016.3388445
Produced by FastQC (version 0.11.5)