##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10473107
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82770585653331	31.0	31.0	34.0	30.0	34.0
2	31.995843162874205	31.0	31.0	34.0	30.0	34.0
3	32.01813740659768	31.0	31.0	34.0	30.0	34.0
4	35.73422901150537	37.0	35.0	37.0	35.0	37.0
5	35.54673345741622	37.0	35.0	37.0	33.0	37.0
6	35.55202243231163	37.0	35.0	37.0	33.0	37.0
7	35.44300616808364	37.0	35.0	37.0	33.0	37.0
8	35.42450478162784	37.0	35.0	37.0	33.0	37.0
9	36.9619652506176	39.0	37.0	39.0	33.0	39.0
10-11	36.85260171599508	39.0	37.0	39.0	33.0	39.0
12-13	36.78957901413592	39.0	37.0	39.0	32.0	39.0
14-15	37.90150635336772	40.0	37.0	41.0	33.0	41.0
16-17	37.88610137373752	40.0	37.0	41.0	33.0	41.0
18-19	37.92571483323907	40.0	37.0	41.0	33.0	41.0
20-21	37.90449081083814	40.0	37.0	41.0	32.5	41.0
22-23	37.83751510083742	40.0	37.0	41.0	32.0	41.0
24-25	37.7469158545696	39.5	37.0	41.0	32.0	41.0
26-27	37.55412317289476	39.0	36.5	41.0	32.0	41.0
28-29	37.44603461368035	39.0	36.0	41.0	32.0	41.0
30-31	37.29827864826535	39.0	36.0	40.5	31.0	41.0
32-33	37.46122580526114	39.0	36.0	41.0	32.0	41.0
34-35	37.41759251578397	39.0	36.0	41.0	31.5	41.0
36-37	37.32965627724093	39.0	36.0	41.0	31.0	41.0
38-39	37.22588483580928	39.0	36.0	41.0	31.0	41.0
40-41	37.13665777995282	39.0	36.0	41.0	31.0	41.0
42-43	37.095526831215395	39.0	36.0	40.0	31.0	41.0
44-45	37.12152165103132	39.0	35.5	40.5	31.0	41.0
46-47	37.22306850563348	39.0	36.0	41.0	31.0	41.0
48-49	37.16876830196773	39.0	36.0	41.0	31.0	41.0
50-51	37.06225066587248	39.0	35.5	41.0	31.0	41.0
52-53	36.96656025297533	39.0	35.0	40.5	31.0	41.0
54-55	36.84672341287312	39.0	35.0	40.0	31.0	41.0
56-57	36.724899810698815	39.0	35.0	40.0	30.0	41.0
58-59	36.58664593794083	38.0	35.0	40.0	30.0	41.0
60-61	36.373886776612224	38.0	35.0	40.0	30.0	41.0
62-63	36.16409231125209	38.0	34.0	40.0	29.0	41.0
64-65	35.96468899431326	37.5	34.0	40.0	29.0	41.0
66-67	35.71361749024912	37.0	34.0	40.0	29.0	41.0
68-69	35.44154743876065	37.0	34.0	39.0	29.0	41.0
70-71	35.151588290808604	36.0	34.0	39.0	29.0	40.5
72-73	34.853265171710206	36.0	33.0	39.0	28.5	40.0
74-75	34.506938959304904	35.0	33.0	38.0	28.0	40.0
76-77	33.45529426555261	34.5	31.5	37.0	26.5	39.0
78-79	33.86166278231043	35.0	32.5	37.0	28.0	39.0
80-81	33.75575519084758	35.0	33.0	36.5	28.0	39.0
82-83	33.521688079421565	35.0	32.5	36.0	28.0	38.5
84-85	33.292300468575945	35.0	32.0	36.0	28.0	37.0
86-87	33.07972599003848	35.0	32.0	35.0	28.0	37.0
88-89	32.8716936283366	34.0	32.0	35.0	27.0	36.5
90-91	32.74094759245992	34.0	32.0	35.0	27.0	36.0
92-93	32.662748128757016	34.0	32.0	35.0	27.0	36.0
94-95	32.62242386528334	34.0	32.0	35.0	27.0	35.5
96-97	32.630923429509636	34.0	32.0	35.0	28.0	35.0
98-99	32.74978752910691	34.0	32.0	35.0	29.0	35.0
100-101	32.121093199607536	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	25.0
20	114.0
21	661.0
22	2671.0
23	8881.0
24	22438.0
25	46939.0
26	82980.0
27	128337.0
28	182243.0
29	240954.0
30	305130.0
31	377059.0
32	463644.0
33	574566.0
34	736901.0
35	961325.0
36	1328657.0
37	1920403.0
38	2091059.0
39	965683.0
40	32434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.7832480848329	22.523029698827674	15.687044923727028	24.0066772926124
2	13.678274701779872	22.890430439397914	41.543001687145754	21.888293171676462
3	16.593156166551147	28.4165529866161	31.8410668390956	23.14922400773715
4	11.916712012968071	19.689601185207025	39.838445267483664	28.555241534341242
5	12.192697390124515	38.892315125266094	36.42705768728567	12.487929797323716
6	28.006741456952554	39.93382288560596	19.363136459887215	12.69629919755427
7	24.498995379308163	33.614924396361076	24.95161178053466	16.934468443796096
8	23.461366335701523	37.80189584618967	21.97888363023504	16.75785418787376
9	25.310674282235446	16.66451035017593	22.05437221256309	35.970443155025535
10-11	23.50050467354148	27.474778974376946	29.862379903117574	19.162336448964
12-13	24.337801571205183	25.18038343349304	29.837912474302037	20.643902520999738
14-15	21.566947611630436	26.379903308540626	27.468281380110028	24.58486769971891
16-17	20.936986512216478	29.35411143990031	28.188048685075024	21.52085336280819
18-19	21.028586454862527	28.618059724978494	29.853361076864555	20.499992743294424
20-21	21.92796585387117	27.78085540924512	29.590739840589457	20.700438896294248
22-23	21.670533048865394	27.681986606272062	29.45845166732569	21.189028677536847
24-25	21.414859097956	27.886039942648942	29.46447890216417	21.234622057230883
26-27	21.295578440118447	28.036851421899232	29.404717762305644	21.262852375676676
28-29	21.25780988785743	28.129086179998886	29.40681482053899	21.206289111604693
30-31	21.579936748711393	27.95252083438074	29.285442387939643	21.18210002896823
32-33	21.369851754298494	28.153394037227276	29.2123666640149	21.264387544459332
34-35	21.499145065896993	28.185689528637592	29.247293736547704	21.06787166891771
36-37	21.472781745892156	28.14231487411553	29.19025465090171	21.194648729090602
38-39	21.32945433142628	28.212030145959073	29.23978078506583	21.218734737548818
40-41	21.52449187674386	28.06103407235785	29.169949969069176	21.24452408182912
42-43	21.39847780977465	28.19969526392454	28.875176179424226	21.52665074687658
44-45	21.512118470319614	28.31644532431908	28.72675702099024	21.44467918437107
46-47	21.56524111849101	28.28809520894945	28.515203076704115	21.631460595855422
48-49	21.472711067363544	28.451943616211313	28.439547101667145	21.635798214757997
50-51	21.550967492676282	28.495105319044416	28.329736787728226	21.624190400551075
52-53	21.63161716637213	28.40177805545024	28.265588740493676	21.70101603768396
54-55	21.57332799368148	28.34872697917944	28.31137804422315	21.766566982915926
56-57	21.61269247545926	28.45175015901569	28.229637521439777	21.70591984408528
58-59	21.561218049344504	28.50898242829213	28.272778595873284	21.657020926490087
60-61	21.702933320070503	28.470951955666568	28.24213517098211	21.58397955328082
62-63	21.620378783267498	28.567254534411678	28.217768389231416	21.594598293089405
64-65	21.58310378308067	28.465055282225045	28.220369590840917	21.731471343853368
66-67	21.693444705404605	28.49923830534955	28.072393552568304	21.73492343667754
68-69	21.58236544464476	28.474301575518375	28.210510602588517	21.732822377248354
70-71	21.639474379374697	28.256774251827675	28.254156584313172	21.849594784484452
72-73	21.63603879152443	28.40467234891168	28.141736485467984	21.817552374095904
74-75	21.52313398326796	28.46484475341342	28.15496930210578	21.857051961212832
76-77	21.610312998486513	28.36437907830473	28.16784329085647	21.857464632352283
78-79	21.58763930015852	28.372603434647363	28.123662074483345	21.916095190710767
80-81	21.65428446502793	28.362133993182265	28.102386207792712	21.881195333997095
82-83	21.652477738353763	28.326348006608786	28.227872584836426	21.79330167020102
84-85	21.695732668997163	28.323824782327055	28.174421650364046	21.80602089831174
86-87	21.54739242669	28.398843209084713	28.239297186808678	21.81446717741661
88-89	21.624362481330476	28.251232106563233	28.228938935545994	21.895466476560298
90-91	21.682284052293483	28.22252707124665	28.292746750333908	21.80244212612596
92-93	21.690085692906134	28.147723030617204	28.304801581610818	21.857389694865848
94-95	21.481299423380804	28.1908080149897	28.39821703199588	21.929675529633613
96-97	21.412192610785024	28.24255248436302	28.50197739069608	21.843277514155872
98-99	21.49831079739906	28.870259666288156	28.566120717146614	21.065308819166173
100-101	22.000744971312848	31.618966355791116	25.884527005122898	20.495761667773138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3889.0
1	3187.5
2	3489.0
3	4746.5
4	6184.5
5	9233.5
6	11824.0
7	14177.5
8	16050.5
9	16294.0
10	15223.5
11	13974.5
12	15152.0
13	19681.0
14	30291.5
15	44973.0
16	56979.0
17	63219.0
18	64375.5
19	62523.0
20	59615.0
21	57746.5
22	58461.0
23	63629.0
24	74930.5
25	92396.0
26	114590.0
27	139097.5
28	165788.0
29	195092.0
30	223774.0
31	252511.0
32	282084.0
33	309747.0
34	333467.5
35	356636.0
36	381542.5
37	402867.0
38	417045.5
39	424902.0
40	430966.5
41	435238.5
42	435083.5
43	435768.5
44	437169.0
45	436024.0
46	431587.0
47	425002.0
48	417538.5
49	405653.5
50	391401.0
51	375317.5
52	354118.5
53	331137.5
54	309046.5
55	286366.0
56	263070.0
57	237644.5
58	210737.5
59	182571.0
60	153505.0
61	125940.0
62	100081.5
63	77224.0
64	58390.0
65	44232.5
66	33493.5
67	25328.5
68	19502.5
69	14194.0
70	9967.0
71	7154.0
72	5008.5
73	3474.0
74	2353.5
75	1541.5
76	1010.0
77	676.5
78	471.5
79	329.5
80	229.5
81	166.0
82	125.0
83	90.5
84	68.0
85	50.0
86	40.0
87	32.0
88	25.5
89	21.5
90	18.0
91	14.5
92	13.0
93	8.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003723823312413403
3	0.0
4	0.0
5	1.909652980724822E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.341892716268439E-4
20-21	5.633760587232647E-4
22-23	2.4352346945499448E-4
24-25	1.5762059014008697E-4
26-27	0.0024897568726130474
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03368749848939057
38-39	0.016996344663220996
40-41	0.022210902363196693
42-43	0.010464738697908373
44-45	0.004059842785222775
46-47	0.005048618347054926
48-49	0.009142364499134867
50-51	0.006925379734728342
52-53	0.01112525441147824
54-55	0.003414037323379895
56-57	0.016209894983037505
58-59	0.012701949884896037
60-61	0.012728721792625575
62-63	0.004781546894285329
64-65	0.006381476461311765
66-67	4.2655855966530504E-4
68-69	8.011241558586469E-4
70-71	0.0011218449207156553
72-73	7.107490187073288E-5
74-75	0.0036257576948152924
76-77	0.004679784099468963
78-79	0.00376627090774291
80-81	6.396760981263134E-4
82-83	0.0031800202450667624
84-85	0.004518531676590174
86-87	0.0013818444978801517
88-89	0.07265133641085118
90-91	7.784674802380226E-4
92-93	0.0
94-95	4.354463928727277E-4
96-97	0.011806334700631221
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	331.0
20-21	1071.0
22-23	2120.0
24-25	5172.0
26-27	6426.0
28-29	9747.0
30-31	26156.0
32-33	33928.0
34-35	48462.0
36-37	74611.0
38-39	86366.0
40-41	88170.0
42-43	99155.0
44-45	116614.0
46-47	117970.0
48-49	107785.0
50-51	97851.0
52-53	94457.0
54-55	102121.0
56-57	108332.0
58-59	109617.0
60-61	107783.0
62-63	101972.0
64-65	107217.0
66-67	115161.0
68-69	117658.0
70-71	117109.0
72-73	112662.0
74-75	112713.0
76-77	115376.0
78-79	124004.0
80-81	128245.0
82-83	122805.0
84-85	123375.0
86-87	131289.0
88-89	139893.0
90-91	152500.0
92-93	158926.0
94-95	185326.0
96-97	249145.0
98-99	836509.0
100-101	5776977.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.7174563681163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.53744906707041	77.6063984008716
2	6.62388644715885	11.355653950433911
3	1.5260592917732485	3.9242976227319573
4	0.577255578318515	1.9792351939107624
5	0.26908481194598755	1.1532632813651482
6	0.14363941642554579	0.7387443246119045
7	0.09250197044226009	0.555032353074445
8	0.05662749222277772	0.3883171675073435
9	0.03934953102404938	0.3035647537793813
>10	0.13068154747062646	1.7294174505489412
>50	0.002678514138855188	0.15094710213105553
>100	7.496659943478983E-4	0.09727664270665254
>500	3.6666014591220035E-5	0.017851756326844343
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.909652980724822E-5
2	0.0	9.54826490362411E-6	0.0	0.0	1.909652980724822E-5
3	0.0	9.54826490362411E-6	0.0	9.54826490362411E-6	1.909652980724822E-5
4	0.0	8.5934384132617E-5	0.0	2.864479471087233E-5	1.909652980724822E-5
5	0.0	9.548264903624111E-5	0.0	2.864479471087233E-5	1.909652980724822E-5
6	0.0	9.548264903624111E-5	0.0	3.819305961449644E-5	1.909652980724822E-5
7	0.0	9.548264903624111E-5	0.0	3.819305961449644E-5	1.909652980724822E-5
8	0.0	1.2412744374711344E-4	0.0	3.819305961449644E-5	1.909652980724822E-5
9	0.0	1.814170331688581E-4	0.0	6.683785432536877E-5	1.909652980724822E-5
10-11	4.774132451812055E-6	1.814170331688581E-4	0.0	1.0025678148805315E-4	1.909652980724822E-5
12-13	9.54826490362411E-6	1.814170331688581E-4	0.0	1.1457917884348932E-4	1.909652980724822E-5
14-15	9.54826490362411E-6	2.3870662259060276E-4	0.0	1.3367570865073755E-4	2.864479471087233E-5
16-17	9.54826490362411E-6	2.3870662259060276E-4	0.0	1.4799810600617372E-4	2.864479471087233E-5
18-19	9.54826490362411E-6	2.5302901994603895E-4	0.0	1.71868768265234E-4	2.864479471087233E-5
20-21	9.54826490362411E-6	3.0077034446415946E-4	0.0	1.814170331688581E-4	2.864479471087233E-5
22-23	4.296719206630849E-5	3.5805993388590414E-4	0.0	2.148359603315425E-4	3.819305961449644E-5
24-25	7.638611922899289E-5	4.0102712595221267E-4	0.0	2.4825488749422687E-4	4.7741324518120556E-5
26-27	8.116025168080494E-5	4.201236557594609E-4	0.0	3.0554447691597155E-4	7.638611922899289E-5
28-29	8.5934384132617E-5	4.201236557594609E-4	0.0	8.593438413261699E-4	7.638611922899289E-5
30-31	9.548264903624111E-5	4.29671920663085E-4	0.0	0.002009909762212875	7.638611922899289E-5
32-33	1.1457917884348932E-4	4.29671920663085E-4	0.0	0.005638250425590038	9.548264903624111E-5
34-35	1.1935331129530138E-4	4.630908478257694E-4	0.0	0.009997033354094444	1.1457917884348932E-4
36-37	1.2412744374711344E-4	4.965097749884537E-4	0.0	0.016255920998420048	1.1457917884348932E-4
38-39	1.2412744374711344E-4	5.15606304795702E-4	0.0	0.025641865398682552	1.1457917884348932E-4
40-41	1.2412744374711344E-4	5.442510995065744E-4	0.0	0.03629772903112706	1.1935331129530138E-4
42-43	1.2412744374711344E-4	5.537993644101984E-4	0.0	0.04564548037177506	1.289015761989255E-4
44-45	1.2412744374711344E-4	6.397337485428154E-4	0.0	0.05649708343474387	1.384498411025496E-4
46-47	1.2412744374711344E-4	7.161198677718083E-4	0.0	0.06851357481595481	1.4322397355436166E-4
48-49	1.2412744374711344E-4	7.161198677718083E-4	0.0	0.0799571703029483	1.4322397355436166E-4
50-51	1.2412744374711344E-4	8.259249141634856E-4	0.0	0.09043161690222395	1.4322397355436166E-4
52-53	1.384498411025496E-4	8.354731790671097E-4	0.0	0.10115431838899383	1.5277223845798577E-4
54-55	1.4322397355436166E-4	8.402473115189218E-4	0.0	0.11186747161086008	1.6709463581342194E-4
56-57	1.4799810600617372E-4	8.497955764225458E-4	0.0	0.12012672075249493	1.7664290071704605E-4
58-59	1.5277223845798577E-4	8.5934384132617E-4	0.0	0.12801836169534025	1.814170331688581E-4
60-61	1.6232050336160989E-4	8.64117973777982E-4	0.0	0.13612483859851715	1.814170331688581E-4
62-63	1.6232050336160989E-4	8.927627684888544E-4	0.0	0.14395918995194074	1.9096529807248222E-4
64-65	1.6232050336160989E-4	9.54826490362411E-4	0.0	0.15126838673566498	1.9096529807248222E-4
66-67	1.6232050336160989E-4	9.54826490362411E-4	0.0	0.15886403146649797	1.9096529807248222E-4
68-69	1.71868768265234E-4	9.786971526214714E-4	0.0	0.16693231531006034	1.9096529807248222E-4
70-71	1.7664290071704605E-4	9.977936824287195E-4	0.0	0.17466163574954405	2.1006182787973045E-4
72-73	1.8619116562067017E-4	0.0010025678148805315	0.0	0.18086800793689972	2.148359603315425E-4
74-75	1.9096529807248222E-4	0.001026438477139592	0.0	0.18647283943532705	2.1961009278335456E-4
76-77	1.9096529807248222E-4	0.001040760874495028	0.0	0.19139974412559713	2.1961009278335456E-4
78-79	1.9096529807248222E-4	0.0010503091393986523	0.0	0.19581581664352327	2.2915835768697865E-4
80-81	1.9096529807248222E-4	0.0010550832718504643	0.0	0.19815036741245937	2.2915835768697865E-4
82-83	1.9096529807248222E-4	0.0010598574043022763	0.0	0.19945370557180403	2.3870662259060276E-4
84-85	1.9096529807248222E-4	0.0010789539341095244	0.0	0.20072362480398603	2.3870662259060276E-4
86-87	1.9096529807248222E-4	0.0010789539341095244	0.0	0.20122968284387813	2.4348075504241481E-4
88-89	1.9096529807248222E-4	0.0011935331129530138	0.0	0.20130606896310713	2.4825488749422687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13530	0.0	50.677113	1
GTATCAA	24155	0.0	35.048763	1
TCAACGC	30355	0.0	27.383026	4
ATCAACG	30415	0.0	27.356283	3
CAACGCA	30975	0.0	26.821533	5
TATCAAC	31610	0.0	26.428452	2
AACGCAG	31915	0.0	26.174511	6
ACGCAGA	36840	0.0	22.57401	7
CGCAGAG	37415	0.0	22.216005	8
GTACATG	33950	0.0	21.320257	1
TACATGG	34355	0.0	20.634258	2
ACATGGG	34550	0.0	20.156569	3
GCAGAGT	43685	0.0	18.80902	9
GAGTACT	24740	0.0	18.282682	12-13
CATGGGG	24385	0.0	18.200151	4
AGAGTAC	40325	0.0	17.717354	10-11
CAGAGTA	43290	0.0	17.246416	10-11
GTGGTAT	6155	0.0	17.184973	1
TGGTATC	5760	0.0	16.923187	2
ATGGGGG	15130	0.0	16.338844	5
>>END_MODULE