FastQCFastQC Report
Fri 27 May 2016
SRR2139856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33008746
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA517850.055.155931
GTATCAA974500.037.648991
ATCAACG1212050.029.7913
TCAACGC1214800.029.785924
TATCAAC1239200.029.3182932
CAACGCA1231250.029.2990995
AACGCAG1268800.028.5080816
ACGCAGA1482200.024.311317
CGCAGAG1495700.024.0862648
GCAGAGT1703850.020.8872789
GAGTACT979700.019.79304712-13
GTACATG1235900.019.4263271
TACATGG1226200.019.3122982
CAGAGTA1678200.018.81393410-11
ACATGGG1235500.018.7002723
GTGGTAT206000.018.386271
GTACTTT1059450.018.24819814-15
AGAGTAC1578700.017.97671510-11
TGGTATC195950.017.7829342
AGTACTT1060700.016.81966612-13
Produced by FastQC (version 0.11.5)