##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139856_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33008746 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98594590657882 31.0 30.0 33.0 28.0 34.0 2 31.3994716127659 31.0 31.0 34.0 28.0 34.0 3 31.534242742817312 31.0 31.0 34.0 28.0 34.0 4 35.2031530370769 37.0 35.0 37.0 33.0 37.0 5 35.24966607334917 37.0 35.0 37.0 33.0 37.0 6 35.13500485598574 37.0 35.0 37.0 32.0 37.0 7 35.157147684434904 37.0 35.0 37.0 32.0 37.0 8 35.255557057514395 37.0 35.0 37.0 32.0 37.0 9 36.98365227203723 39.0 37.0 39.0 33.0 39.0 10-11 36.95821145401888 39.0 37.0 39.0 33.0 39.0 12-13 36.87260885342327 39.0 37.0 39.0 32.5 39.0 14-15 38.06402665220908 40.0 37.5 41.0 33.0 41.0 16-17 38.10456140927014 40.0 37.5 41.0 33.0 41.0 18-19 38.157683118286286 40.0 38.0 41.0 33.0 41.0 20-21 38.17125955059682 40.0 38.0 41.0 33.0 41.0 22-23 38.119126086136944 40.0 38.0 41.0 33.0 41.0 24-25 38.14442333055585 40.0 38.0 41.0 33.0 41.0 26-27 38.03303470714251 40.0 37.0 41.0 33.0 41.0 28-29 37.99227859209749 40.0 37.0 41.0 33.0 41.0 30-31 37.91471265147054 40.0 37.0 41.0 33.0 41.0 32-33 37.820058684675374 40.0 37.0 41.0 33.0 41.0 34-35 37.73921363117509 40.0 37.0 41.0 33.0 41.0 36-37 37.642016127751006 40.0 37.0 41.0 32.0 41.0 38-39 37.617613215543514 40.0 37.0 41.0 32.0 41.0 40-41 37.54083211921096 40.0 37.0 41.0 32.0 41.0 42-43 37.49034727977329 40.0 36.0 41.0 31.5 41.0 44-45 37.42275535106309 40.0 36.0 41.0 31.0 41.0 46-47 37.29108667007925 39.0 36.0 41.0 31.0 41.0 48-49 37.214879517098204 39.0 36.0 41.0 31.0 41.0 50-51 37.13847495155379 39.0 36.0 41.0 31.0 41.0 52-53 37.048846569480425 39.0 35.5 41.0 31.0 41.0 54-55 36.901412917145535 39.0 35.0 41.0 30.5 41.0 56-57 36.7510771491671 39.0 35.0 40.0 30.0 41.0 58-59 36.56949157553346 38.5 35.0 40.0 30.0 41.0 60-61 36.39745072661056 38.0 35.0 40.0 30.0 41.0 62-63 36.209554540599655 38.0 34.5 40.0 30.0 41.0 64-65 36.02176512220907 37.5 34.0 40.0 29.5 41.0 66-67 35.763673171061484 37.0 34.0 40.0 29.0 41.0 68-69 35.46048029235492 36.5 34.0 39.0 29.0 41.0 70-71 35.16892119734861 36.0 34.0 39.0 29.0 40.5 72-73 34.89361536249477 36.0 34.0 39.0 29.0 40.0 74-75 34.58557601854118 35.0 33.0 38.0 29.0 40.0 76-77 34.28498205915074 35.0 33.0 37.0 29.0 39.0 78-79 34.24161680731478 35.0 33.0 37.0 29.0 39.0 80-81 33.99408559629728 35.0 33.0 36.5 29.0 39.0 82-83 33.7913055668792 35.0 33.0 36.0 29.0 38.5 84-85 33.57678880452906 35.0 33.0 36.0 29.0 37.0 86-87 33.3704946280466 35.0 33.0 35.0 29.0 37.0 88-89 33.21908963644427 35.0 33.0 35.0 29.0 36.5 90-91 33.10622193712649 35.0 33.0 35.0 29.0 36.0 92-93 33.044214697572926 35.0 33.0 35.0 29.0 36.0 94-95 33.00557418181346 35.0 32.5 35.0 29.0 36.0 96-97 33.04676389889916 34.5 33.0 35.0 29.0 35.0 98-99 33.150872905800014 34.0 33.0 35.0 29.0 35.0 100 32.71163788284244 34.0 32.0 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 2.0 15 1.0 16 12.0 17 350.0 18 3309.0 19 11218.0 20 23623.0 21 39771.0 22 62731.0 23 96480.0 24 145646.0 25 213559.0 26 297325.0 27 396849.0 28 509540.0 29 641568.0 30 791514.0 31 981983.0 32 1223376.0 33 1568398.0 34 2081202.0 35 2834460.0 36 4119334.0 37 6268973.0 38 7133708.0 39 3438695.0 40 125118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02512570456327 19.13388045701585 9.843091282534635 28.997902555886252 2 14.396563262354773 22.89176935106835 37.2202203622034 25.491447024373482 3 17.6513824548197 27.391034485224008 29.1695267672392 25.788056292717087 4 12.19831253207862 19.474678014123892 37.87659185841231 30.450417595385176 5 13.159040923712798 38.91629168301449 34.41510438479361 13.509563008479105 6 28.59579768370341 39.769078077408274 18.33920214996649 13.295922088921827 7 25.660753668133896 34.43821222411781 22.59183975059216 17.30919435715613 8 24.995717801579012 36.836100953365516 20.47380715401912 17.69437409103636 9 26.273506421601112 16.81069920075122 20.739218630117 36.17657574753067 10-11 23.87819579695636 27.425969165868946 28.557417782547688 20.138417254627 12-13 24.981736658520745 25.269261970751632 28.767728710445407 20.98127266028222 14-15 22.266188179338894 26.105313119135154 26.83346559121028 24.79503311031567 16-17 21.282536755561694 29.06669038563295 27.558178065898048 22.09259479290731 18-19 21.27560374453486 28.179316475700105 29.522874028598363 21.022205751166677 20-21 22.35584854501292 27.229618756592977 29.38972243776335 21.02481026063075 22-23 22.015426396594652 27.140453767098627 29.29599409837201 21.548125737934715 24-25 21.636936939821 27.288088181738885 29.446076109522856 21.628898768917256 26-27 21.57479345998687 27.475543404152113 29.32704341699851 21.622619718862506 28-29 21.571273741137734 27.44168386055559 29.409490491596628 21.577551906710045 30-31 21.846198933709076 27.33091177189806 29.302829495136677 21.520059799256188 32-33 21.60595890741638 27.494582188205683 29.308092139032745 21.591366765345192 34-35 21.776173230784284 27.514353504036755 29.29852632283245 21.410946942346502 36-37 21.784554860346095 27.386851416174036 29.24134440628067 21.587249317199195 38-39 21.62165808555134 27.558242974620047 29.261374540861667 21.55872439896695 40-41 21.87906520174736 27.418828754129475 29.124710145489836 21.57739589863333 42-43 21.773239034680742 27.51353178109201 28.89283610107781 21.820393083149444 44-45 21.807089561004027 27.739789155624056 28.68534781654939 21.767773466822522 46-47 21.971412049707588 27.73769863742138 28.292792780267394 21.998096532603633 48-49 21.91420305512369 27.865639516559366 28.191951353687177 22.02820607462976 50-51 21.898068351813997 28.02941150664065 28.049255999617547 22.023264141927815 52-53 22.04413777386979 27.932069218776462 27.97089600564984 22.052897001703904 54-55 22.00838447083079 27.826608253230905 28.070919729071935 22.094087546866366 56-57 21.99006489021265 27.97216344105589 28.030121500942812 22.00765016778865 58-59 21.991081915250962 27.991954816413667 28.023521334769313 21.993441933566064 60-61 22.11416448016242 27.936393118914765 27.99825896676424 21.951183434158576 62-63 22.0398634390535 28.039441436949513 27.985904915213887 21.93479020878309 64-65 22.006425884791287 27.955262055968642 27.963434946228123 22.074877113011944 66-67 22.034755531510772 28.067654770616173 27.8339484867585 22.06364121111455 68-69 21.98960934314204 27.974654374806278 27.94275577865224 22.092980503399442 70-71 22.018027764394066 27.755357699253697 27.975111391443715 22.251503144908526 72-73 22.042456971471843 27.814961614246464 27.943282708288645 22.199298705993048 74-75 21.89335963776096 27.9880599770869 27.9548702288912 22.163710156260944 76-77 22.040972960644986 27.936329486788363 27.871183938107748 22.1515136144589 78-79 21.989793356095607 27.94639664919672 27.87143059013607 22.192379404571604 80-81 22.0186588147515 27.997393564234898 27.824191667107367 22.159755953906238 82-83 22.0322452127994 27.935282877574107 27.939473171727787 22.092998737898707 84-85 22.09108719313097 27.971291329060342 27.848554359656546 22.089067118152133 86-87 21.917786370047445 28.055664443014066 27.97630652392548 22.050242663013005 88-89 21.99012243281284 27.982418084957462 27.868405308179362 22.15905417405034 90-91 21.977318993686687 28.000518298544584 27.941220171136166 22.080942536632563 92-93 22.009658037852965 28.03142829645581 27.93109296871887 22.027820696972356 94-95 21.84756150928016 28.06662153105044 27.99713113977504 22.088685819894362 96-97 21.884519036192028 28.206604923285937 28.064371402969453 21.844504637552582 98-99 22.859491447190386 30.14663759338316 28.286718092454755 18.707152866971693 100 22.062743903302366 30.99803778832037 22.097227857254104 24.84199045112316 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 22349.0 1 18741.0 2 11507.0 3 7793.0 4 8175.5 5 12051.0 6 19051.0 7 29976.5 8 44753.0 9 53769.0 10 51156.5 11 46302.0 12 50564.5 13 66862.5 14 106177.0 15 160449.0 16 204832.5 17 231257.0 18 237974.5 19 226165.5 20 203228.0 21 179049.5 22 164943.5 23 172028.0 24 200058.5 25 243632.5 26 303024.5 27 370074.5 28 434350.0 29 505287.0 30 581655.0 31 656742.0 32 738370.5 33 818152.0 34 893485.0 35 964281.0 36 1039612.0 37 1110841.0 38 1162780.5 39 1201527.0 40 1235907.5 41 1266150.5 42 1289033.5 43 1315000.0 44 1332760.0 45 1347738.5 46 1356147.5 47 1358538.0 48 1358179.5 49 1336438.5 50 1305795.0 51 1271502.5 52 1218640.0 53 1149218.5 54 1080912.0 55 1008689.0 56 932562.0 57 850497.5 58 767318.0 59 681837.0 60 577697.0 61 466946.5 62 365635.5 63 284436.0 64 218211.5 65 163846.5 66 122430.5 67 93414.0 68 71741.0 69 53091.0 70 39023.0 71 28710.0 72 21167.5 73 15535.5 74 11110.0 75 7876.0 76 5592.5 77 3835.5 78 2631.0 79 1887.5 80 1332.5 81 925.0 82 685.5 83 482.0 84 335.5 85 261.5 86 204.5 87 165.5 88 138.5 89 107.5 90 74.5 91 55.5 92 50.0 93 38.5 94 32.0 95 39.5 96 43.0 97 29.5 98 16.5 99 20.0 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.14601584683041277 6 0.00633771425306493 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.5152571699014528E-6 22-23 4.7910471218587977E-4 24-25 3.8688668371233764E-4 26-27 0.0031887205055500744 28-29 6.399735570830875E-4 30-31 1.3706202553124403E-5 32-33 1.5268538085574202E-6 34-35 6.127135049991141E-6 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.002619839031625202 48-49 0.0 50-51 3.1160764386014696E-5 52-53 0.0 54-55 6.671576390925689E-6 56-57 1.800250012664843E-4 58-59 8.659052507119142E-5 60-61 1.439774959060256E-4 62-63 0.0 64-65 6.980852620745583E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.786581787385423E-4 76-77 3.147037535290555E-5 78-79 3.217095977214132E-4 80-81 0.0 82-83 0.0 84-85 7.739750277731265E-6 86-87 0.0 88-89 6.736776030630139E-4 90-91 0.0 92-93 1.3057837180236777E-4 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 7090.0 20-21 17834.0 22-23 22658.0 24-25 25761.0 26-27 31765.0 28-29 51785.0 30-31 82312.0 32-33 97636.0 34-35 134233.0 36-37 196114.0 38-39 254008.0 40-41 254829.0 42-43 288945.0 44-45 347547.0 46-47 336197.0 48-49 303470.0 50-51 270085.0 52-53 245503.0 54-55 256548.0 56-57 267765.0 58-59 274866.0 60-61 274960.0 62-63 254090.0 64-65 257067.0 66-67 275623.0 68-69 285929.0 70-71 288651.0 72-73 267346.0 74-75 261581.0 76-77 272111.0 78-79 292597.0 80-81 305724.0 82-83 294085.0 84-85 288532.0 86-87 306298.0 88-89 339542.0 90-91 373298.0 92-93 401594.0 94-95 481921.0 96-97 1147178.0 98-99 2091678.0 100-101 2.048199E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.91265751316584 #Duplication Level Percentage of deduplicated Percentage of total 1 87.50686170051402 69.92905869125146 2 8.186014227731365 13.083323027571984 3 2.1454499867514647 5.14345830008688 4 0.9048615981588511 2.8923957996193668 5 0.4392891495947631 1.755238168040809 6 0.23779596384117524 1.1401744449871805 7 0.1481808669284004 0.8289068808197285 8 0.09493881825487996 0.6069450614325536 9 0.06665538868090928 0.4793948322358009 >10 0.26015195442922795 3.2549698109476797 >50 0.006736350159067504 0.36260100157733416 >100 0.0029422076911773073 0.40947698987516634 >500 8.453948399403952E-5 0.047809775452622265 >1k 3.724778087373714E-5 0.0662472161014545 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0295001209679397E-6 2 0.0 3.0295001209679398E-5 0.0 3.0295001209679397E-6 3.0295001209679397E-6 3 3.0295001209679397E-6 3.0295001209679398E-5 0.0 1.5147500604839699E-5 3.0295001209679397E-6 4 3.0295001209679397E-6 6.967850278226262E-5 0.0 3.0295001209679398E-5 3.0295001209679397E-6 5 3.0295001209679397E-6 6.967850278226262E-5 0.0 3.0295001209679398E-5 3.0295001209679397E-6 6 3.0295001209679397E-6 6.967850278226262E-5 0.0 3.9383501572583216E-5 6.059000241935879E-6 7 3.0295001209679397E-6 6.967850278226262E-5 0.0 4.241300169355116E-5 6.059000241935879E-6 8 3.0295001209679397E-6 7.573750302419849E-5 0.0 4.54425018145191E-5 1.5147500604839699E-5 9 3.0295001209679397E-6 1.302685052016214E-4 0.0 9.694400387097407E-5 2.120650084677558E-5 10-11 6.059000241935879E-6 1.302685052016214E-4 0.0 1.454160058064611E-4 2.120650084677558E-5 12-13 7.573750302419849E-6 1.3329800532258933E-4 0.0 1.7268150689517256E-4 2.4236000967743517E-5 14-15 1.060325042338779E-5 1.620782564717848E-4 0.0 1.7722575707662447E-4 3.180975127016337E-5 16-17 1.2118000483871759E-5 1.7571100701614052E-4 0.0 1.9994700798388402E-4 4.8472001935487035E-5 18-19 1.2118000483871759E-5 1.908585076209802E-4 0.0 2.4690425985888706E-4 5.301625211693895E-5 20-21 1.2118000483871759E-5 2.105502584072718E-4 0.0 2.832582613105024E-4 5.453100217742292E-5 22-23 1.3632750544355728E-5 2.4841900991937104E-4 0.0 3.180975127016337E-4 6.210475247984277E-5 24-25 1.5147500604839699E-5 2.862877614314703E-4 0.0 3.5445151415324893E-4 7.876700314516643E-5 26-27 1.817700072580764E-5 2.9992051197582606E-4 0.0 3.9989401596776805E-4 8.179650326613437E-5 28-29 1.817700072580764E-5 3.0143526203631004E-4 0.0 7.058735281855299E-4 8.482600338710232E-5 30-31 2.120650084677558E-5 3.362745134274413E-4 0.0 0.0017010643179234983 8.482600338710232E-5 32-33 2.120650084677558E-5 3.377892634879253E-4 0.0 0.003355171383971993 8.785550350807026E-5 34-35 2.4236000967743517E-5 4.0595301620970396E-4 0.0 0.005377362714718093 9.694400387097407E-5 36-37 2.4236000967743517E-5 4.741167689314826E-4 0.0 0.008349302333387643 1.0148825405242599E-4 38-39 2.4236000967743517E-5 4.892642695363223E-4 0.0 0.013410082285464586 1.060325042338779E-4 40-41 2.4236000967743517E-5 5.347067713508414E-4 0.0 0.018875300503690746 1.0906200435484584E-4 42-43 2.5750751028227488E-5 5.695460227419727E-4 0.0 0.023537701189860406 1.105767544153298E-4 44-45 3.0295001209679398E-5 6.422540256452033E-4 0.0 0.028671189144840584 1.1512100459678171E-4 46-47 3.0295001209679398E-5 6.937555277016582E-4 0.0 0.03426516111820788 1.2269475489920157E-4 48-49 3.0295001209679398E-5 7.043587781250459E-4 0.0 0.03929716081913563 1.3329800532258936E-4 50-51 3.0295001209679398E-5 8.543190341129591E-4 0.0 0.04447457652586984 1.3329800532258936E-4 52-53 3.0295001209679398E-5 8.618927844153789E-4 0.0 0.04983224748980164 1.560192562298489E-4 54-55 3.0295001209679398E-5 8.740107848992506E-4 0.0 0.05502935494732214 1.7268150689517256E-4 56-57 3.332450133064734E-5 8.937025356855423E-4 0.0 0.059905335392020045 1.7268150689517256E-4 58-59 3.332450133064734E-5 8.997615359274782E-4 0.0 0.06451926407625422 1.7419625695565654E-4 60-61 3.483925139113131E-5 9.179385366532858E-4 0.0 0.0688014624972424 1.7874050713710844E-4 62-63 3.786875151209925E-5 9.300565371371575E-4 0.0 0.07276707815558943 1.969175078629161E-4 64-65 3.9383501572583216E-5 9.58836788286353E-4 0.0 0.07582384377764609 1.9843225792340005E-4 66-67 3.9383501572583216E-5 9.724695388307087E-4 0.0 0.07871550164310998 2.0449125816533596E-4 68-69 3.9383501572583216E-5 9.891317894960322E-4 0.0 0.08139206499998516 2.0903550834678786E-4 70-71 3.9383501572583216E-5 9.98220289858936E-4 0.0 0.08376416359470305 2.120650084677558E-4 72-73 3.9383501572583216E-5 9.9973503991942E-4 0.0 0.08582573842702174 2.51448510040339E-4 74-75 3.9383501572583216E-5 0.0010330595412500676 0.0 0.0874268292409533 2.51448510040339E-4 76-77 3.9383501572583216E-5 0.001108797044274266 0.0 0.08889310729950178 2.5296326010082297E-4 78-79 3.9383501572583216E-5 0.0011542395460887851 0.0 0.08981104583615507 2.5447801016130695E-4 80-81 3.9383501572583216E-5 0.0011542395460887851 0.0 0.09011248109819137 2.665960106451787E-4 82-83 4.54425018145191E-5 0.001158783796270237 0.0 0.09052903736482446 2.878025114919543E-4 84-85 4.54425018145191E-5 0.0011663575465726567 0.0 0.0907244401226269 2.878025114919543E-4 86-87 4.54425018145191E-5 0.0011845345472984645 0.0 0.09078957437522771 2.878025114919543E-4 88 4.54425018145191E-5 0.0012966260517742782 0.0 0.09079411862540916 2.878025114919543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 51785 0.0 55.15593 1 GTATCAA 97450 0.0 37.64899 1 ATCAACG 121205 0.0 29.791 3 TCAACGC 121480 0.0 29.78592 4 TATCAAC 123920 0.0 29.318293 2 CAACGCA 123125 0.0 29.299099 5 AACGCAG 126880 0.0 28.508081 6 ACGCAGA 148220 0.0 24.31131 7 CGCAGAG 149570 0.0 24.086264 8 GCAGAGT 170385 0.0 20.887278 9 GAGTACT 97970 0.0 19.793047 12-13 GTACATG 123590 0.0 19.426327 1 TACATGG 122620 0.0 19.312298 2 CAGAGTA 167820 0.0 18.813934 10-11 ACATGGG 123550 0.0 18.700272 3 GTGGTAT 20600 0.0 18.38627 1 GTACTTT 105945 0.0 18.248198 14-15 AGAGTAC 157870 0.0 17.976715 10-11 TGGTATC 19595 0.0 17.782934 2 AGTACTT 106070 0.0 16.819666 12-13 >>END_MODULE