FastQCFastQC Report
Fri 27 May 2016
SRR2139855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16332317
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277600.052.376721
GTATCAA505550.035.8067971
ATCAACG660700.026.8884813
TCAACGC662600.026.7818244
TATCAAC672600.026.5558662
CAACGCA672400.026.3827175
AACGCAG694050.025.6081736
ACGCAGA796400.022.2521257
CGCAGAG806850.021.9519778
GTACATG672700.020.276481
TACATGG671600.019.9815732
ACATGGG680600.019.4009173
GCAGAGT904200.019.2449749
GAGTACT500550.018.37034412-13
AGAGTAC836200.018.00825310-11
CAGAGTA883450.017.73210-11
TGGTATC104650.017.5876352
GTGGTAT113600.017.3174881
AGTACTT541700.017.08044812-13
CATGGGG400600.016.9409394
Produced by FastQC (version 0.11.5)