##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16332317
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24492801603104	31.0	31.0	34.0	28.0	34.0
2	31.326210726867473	31.0	31.0	34.0	28.0	34.0
3	31.328616754132312	31.0	31.0	34.0	28.0	34.0
4	35.031611069023455	37.0	35.0	37.0	32.0	37.0
5	34.74667446143741	37.0	35.0	37.0	32.0	37.0
6	34.811859823685765	37.0	35.0	37.0	31.0	37.0
7	34.671671325017755	37.0	35.0	37.0	30.0	37.0
8	34.65792955157556	37.0	35.0	37.0	30.0	37.0
9	36.11721374254492	39.0	35.0	39.0	30.0	39.0
10-11	36.04550992366852	39.0	35.0	39.0	30.0	39.0
12-13	35.999772353181726	39.0	35.0	39.0	30.0	39.0
14-15	37.077899296223556	39.0	36.0	41.0	30.0	41.0
16-17	37.11838543790204	39.0	36.0	41.0	31.0	41.0
18-19	37.18370032861841	39.5	36.0	41.0	31.0	41.0
20-21	37.24188689621706	40.0	36.0	41.0	31.0	41.0
22-23	37.217056777941394	40.0	36.0	41.0	31.0	41.0
24-25	37.188939371142354	39.5	36.0	41.0	30.0	41.0
26-27	37.09825453366275	39.0	36.0	41.0	30.0	41.0
28-29	37.052117723652074	39.0	36.0	41.0	30.0	41.0
30-31	36.9537635359045	39.0	36.0	41.0	30.0	41.0
32-33	36.84788786217895	39.0	35.0	41.0	30.0	41.0
34-35	36.79226511680574	39.0	35.0	41.0	30.0	41.0
36-37	36.77400368200436	39.0	35.0	41.0	30.0	41.0
38-39	36.72368176193055	39.0	35.0	41.0	30.0	41.0
40-41	36.70591018201384	39.0	35.0	41.0	30.0	41.0
42-43	36.67054139681011	39.0	35.0	41.0	30.0	41.0
44-45	36.64057660412945	39.0	35.0	41.0	30.0	41.0
46-47	36.60127059330997	39.0	35.0	41.0	30.0	41.0
48-49	36.53887542258415	39.0	35.0	41.0	29.5	41.0
50-51	36.483410054406136	39.0	35.0	40.0	29.0	41.0
52-53	36.41730913459443	39.0	35.0	40.0	29.0	41.0
54-55	36.32040565457585	38.0	34.5	40.0	29.0	41.0
56-57	36.22379054341866	38.0	34.0	40.0	29.0	41.0
58-59	36.08596769799499	38.0	34.0	40.0	28.5	41.0
60-61	35.916785190916315	38.0	34.0	40.0	28.0	41.0
62-63	35.74959152169103	38.0	34.0	40.0	28.0	41.0
64-65	35.56156889343224	37.0	34.0	40.0	28.0	41.0
66-67	35.34132342927758	37.0	33.5	40.0	28.0	41.0
68-69	35.09839472536777	36.0	33.0	39.0	28.0	41.0
70-71	34.867880402103054	36.0	33.0	39.0	28.0	41.0
72-73	34.58802613229005	35.5	33.0	39.0	28.0	40.0
74-75	34.314231822529095	35.0	33.0	38.0	28.0	40.0
76-77	34.033024850912206	35.0	32.0	37.0	28.0	39.5
78-79	33.76134909694869	35.0	32.0	37.0	27.5	39.0
80-81	33.51157160472763	35.0	32.0	36.5	27.0	39.0
82-83	33.39882143618529	35.0	32.0	36.0	27.5	38.0
84-85	33.3687315311156	35.0	32.0	36.0	28.0	37.0
86-87	33.2140680953876	35.0	32.0	35.5	28.0	37.0
88-89	33.07629925309929	35.0	32.0	35.0	28.0	36.5
90-91	33.001577597218684	35.0	32.0	35.0	28.0	36.0
92-93	32.95486042198027	35.0	32.0	35.0	28.5	36.0
94-95	32.93064174527108	34.5	32.0	35.0	29.0	36.0
96-97	32.98442581885931	34.0	32.0	35.0	29.0	35.5
98-99	33.08351980272377	34.0	32.0	35.0	29.0	35.0
100	32.74387520139111	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	11.0
15	27.0
16	68.0
17	258.0
18	1604.0
19	7952.0
20	25974.0
21	60032.0
22	103094.0
23	145315.0
24	187052.0
25	227610.0
26	269332.0
27	309413.0
28	354329.0
29	403999.0
30	465947.0
31	546466.0
32	656152.0
33	803284.0
34	1017642.0
35	1322375.0
36	1828080.0
37	2690973.0
38	3154421.0
39	1686860.0
40	64044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55119674691595	21.007031641621946	14.387315651539215	24.054455959922894
2	14.492169114767977	22.403520578249857	40.49430953366874	22.610000773313423
3	17.000183664874253	27.986460365101763	31.458381778337746	23.554974191686235
4	12.102762110946538	19.179251078075517	39.30832436149068	29.409662449487268
5	12.048774022271585	39.22966958080428	35.9970988736627	12.724457523261435
6	28.018432824030988	39.75825816600311	19.264777167027162	12.958531842938745
7	24.735663206717554	33.93840037715729	24.34190516238026	16.98403125374489
8	24.289149461679997	36.685342893778554	21.671354858295864	17.35415278624559
9	25.761415724485794	16.096779614061223	21.54135703929566	36.600447622157326
10-11	23.517876286709548	27.19086826899667	29.61604567751727	19.67520976677651
12-13	24.6847055853553	25.119892133740617	29.63457830974524	20.560823971158843
14-15	21.93053266041679	26.10257276622963	27.539659149699602	24.427235423653975
16-17	20.897303596245894	29.130038100199375	28.200154076015977	21.772504227538754
18-19	20.952579467069153	28.176249481386574	30.21866251484043	20.65250853670384
20-21	22.12837638565772	27.182060735682654	30.01831891021099	20.67124396844864
22-23	21.74105192224727	27.11818613898831	29.927267297887795	21.213494640876625
24-25	21.282001529783	27.38346894300277	30.038869440105444	21.295660087108786
26-27	21.248447763293278	27.522901855571586	29.887709829209204	21.340940551925936
28-29	21.22341028250205	27.521694625148218	29.953985731193416	21.300909361156307
30-31	21.535955076715094	27.440399271082693	29.77942387304689	21.24422177915532
32-33	21.297784374849314	27.65287134997989	29.664014347304995	21.385329927865797
34-35	21.49275697371237	27.698598818343722	29.66917901441643	21.139465193527474
36-37	21.462125369969325	27.61259818122082	29.57459321640022	21.350683232409633
38-39	21.342127647300188	27.700361492011567	29.591416874667082	21.366093986021166
40-41	21.561879351622846	27.58529446533578	29.46200516069506	21.39082102234632
42-43	21.46582638364174	27.723795101125337	29.182005094867954	21.628373420364973
44-45	21.544466501809232	27.890169598041346	28.977331829868437	21.58803207028098
46-47	21.684061544383734	27.91893968179171	28.59202537967469	21.804973394149865
48-49	21.66770217566498	28.116010988689915	28.427796757719843	21.78849007792526
50-51	21.670601313645566	28.204691768288313	28.26706139407294	21.857645523993178
52-53	21.79126296887628	28.072703157686263	28.237131541020716	21.89890233241674
54-55	21.723881720100785	28.042839441507443	28.33818772516013	21.89509111323164
56-57	21.756516107289745	28.110818673530545	28.254063303921477	21.87860191525823
58-59	21.742633118992572	28.154915840639898	28.264820903339356	21.837630137028178
60-61	21.845105216903406	28.13925717645648	28.257836814893434	21.757800791746682
62-63	21.754850377907843	28.20463786080145	28.238129329900286	21.802382431390424
64-65	21.708786873810496	28.125936251698885	28.24895823060811	21.91631864388251
66-67	21.821872289891203	28.208824608937206	28.080840894919678	21.888462206251916
68-69	21.773845620642618	28.125979785748374	28.192875778196214	21.907298815412798
70-71	21.790827704931868	27.92239084110743	28.23702555402141	22.049755899939292
72-73	21.824587658631696	28.059417597022478	28.11571549380733	22.000279250538497
74-75	21.6589766927629	28.170695438339838	28.172315805013586	21.998012063883678
76-77	21.802605710962776	28.093438830146034	28.13823751162682	21.96571794726437
78-79	21.742660932409535	28.144500175945797	28.08356109494842	22.02927779669625
80-81	21.772789819466002	28.18221528876329	28.062959777111956	21.982035114658753
82-83	21.83313622058662	28.080983145525074	28.191778447189737	21.894102186698568
84-85	21.839263404827744	28.15543951070752	28.069766555448698	21.935530529016038
86-87	21.675288920547104	28.233303046675857	28.182586386113734	21.908821646663306
88-89	21.770422836006713	28.141347044795424	28.11152663241537	21.976703486782494
90-91	21.799165323589072	28.155536200572428	28.182985348875984	21.862313126962515
92-93	21.76954340171391	28.170222101266994	28.166327315418172	21.893907181600923
94-95	21.594068452790328	28.23258158792143	28.243030534583944	21.930319424704297
96-97	21.624493778597305	28.365515062553133	28.273201085744326	21.73679007310524
98-99	22.563698019926974	30.336713007437044	28.47589843103449	18.623690541601494
100	21.750651134726382	31.25272755561684	22.22853996135146	24.76808134830532
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4192.0
1	4192.0
2	6534.5
3	9004.5
4	11307.0
5	18750.5
6	25468.5
7	30307.0
8	33112.5
9	31198.5
10	25871.0
11	20194.5
12	20659.0
13	28452.5
14	49859.5
15	80826.5
16	105028.0
17	117180.5
18	120285.0
19	116815.5
20	108573.5
21	100350.5
22	97787.5
23	105002.0
24	122340.5
25	149345.0
26	183662.5
27	219235.5
28	254303.0
29	292874.5
30	332371.0
31	371018.0
32	413826.5
33	455661.0
34	494274.5
35	530691.0
36	568690.5
37	603306.5
38	626328.5
39	640269.5
40	651830.5
41	660734.5
42	664158.5
43	673880.5
44	685023.5
45	691230.0
46	691765.5
47	687015.5
48	682486.5
49	671379.0
50	654274.5
51	630468.0
52	598208.0
53	561035.5
54	520619.5
55	481227.0
56	442317.0
57	400107.0
58	359208.5
59	318276.0
60	271536.0
61	221534.5
62	175544.0
63	138568.0
64	108056.0
65	83794.5
66	63861.0
67	48839.5
68	38207.0
69	29259.5
70	22178.5
71	16910.0
72	12913.0
73	9897.5
74	7324.0
75	5380.5
76	3979.0
77	2881.0
78	2124.0
79	1584.0
80	1144.0
81	838.5
82	654.5
83	447.5
84	307.5
85	239.5
86	189.0
87	147.0
88	101.5
89	67.5
90	51.5
91	45.5
92	34.5
93	18.5
94	7.5
95	3.5
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.022434049008478098
4	0.015454022843176506
5	0.08044174014011607
6	0.04873772655771989
7	0.010592495847343644
8	0.005265633773824008
9	0.015141758514728803
10-11	0.020869665951254803
12-13	0.0014725406076798533
14-15	0.00745760690292749
16-17	7.286167663779732E-4
18-19	0.009254657498994172
20-21	0.0020805020070563027
22-23	0.0016105502885277836
24-25	0.011644975506386721
26-27	8.613635914776441E-4
28-29	0.001402497265053273
30-31	0.004913675777056542
32-33	0.024643879785328015
34-35	3.6527591037840024E-4
36-37	9.113213896180507E-5
38-39	9.001955991909523E-4
40-41	0.003310655450225768
42-43	0.014244734566805299
44-45	9.230185508399272E-4
46-47	0.0018284446993869334
48-49	8.741775124357635E-5
50-51	0.0010959235693372973
52-53	4.7825725533459835E-4
54-55	3.545918937581502E-4
56-57	1.3353831229993634E-4
58-59	0.009813321899030265
60-61	0.006848065347807449
62-63	6.112474107941708E-4
64-65	0.010614553597519005
66-67	0.0012274145494447586
68-69	0.00522085967947761
70-71	0.0049627950461700035
72-73	0.02361915456316856
74-75	5.075821647653095E-4
76-77	0.005795049139804553
78-79	6.399479338361031E-5
80-81	6.484577567057827E-5
82-83	0.0
84-85	0.0
86-87	0.01207273315887447
88-89	2.2228468991969708E-4
90-91	1.8235718545057118E-4
92-93	1.3247569159376381E-5
94-95	2.0241138530756927E-4
96-97	3.916279346639284E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	9713.0
20-21	18653.0
22-23	21112.0
24-25	23096.0
26-27	28765.0
28-29	44927.0
30-31	69739.0
32-33	78335.0
34-35	97871.0
36-37	126462.0
38-39	158821.0
40-41	155572.0
42-43	173261.0
44-45	206584.0
46-47	201250.0
48-49	184923.0
50-51	163880.0
52-53	149005.0
54-55	152699.0
56-57	159153.0
58-59	164968.0
60-61	163406.0
62-63	148920.0
64-65	149157.0
66-67	158479.0
68-69	163627.0
70-71	166054.0
72-73	151524.0
74-75	147429.0
76-77	153989.0
78-79	162087.0
80-81	167596.0
82-83	157028.0
84-85	153389.0
86-87	160867.0
88-89	176900.0
90-91	190965.0
92-93	201501.0
94-95	231700.0
96-97	523078.0
98-99	929316.0
100-101	9486516.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.67222029598935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.57254916994138	74.94734066620671
2	7.070184757301689	11.831561130925856
3	1.7817394014273977	4.472462751188322
4	0.7048997516474973	2.3592210922575028
5	0.3262688271585168	1.3649818590860743
6	0.1816173130067642	0.9117794294080575
7	0.10240126730239882	0.5997698977420363
8	0.06465559856679164	0.4327901989319714
9	0.0446949783592064	0.33657552678563946
>10	0.14378115401329414	1.8786971297152986
>50	0.004114064870768212	0.23332026918821613
>100	0.002771866619246518	0.4366584862579857
>500	2.6056682811911424E-4	0.13960362088640585
>1k	6.128295699176318E-5	0.05523794141991887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.28598097869396E-5	0.0	0.0	0.0
3	0.0	4.28598097869396E-5	0.0	0.0	0.0
4	0.0	7.34739596347536E-5	0.0	0.0	0.0
5	0.0	7.34739596347536E-5	0.0	0.0	0.0
6	0.0	7.34739596347536E-5	0.0	0.0	6.1228299695628E-6
7	0.0	7.95967896043164E-5	0.0	0.0	6.1228299695628E-6
8	6.1228299695628E-6	9.1842449543442E-5	0.0	0.0	6.1228299695628E-6
9	6.1228299695628E-6	1.347022593303816E-4	0.0	1.22456599391256E-5	6.1228299695628E-6
10-11	6.1228299695628E-6	1.408250892999444E-4	0.0	1.22456599391256E-5	6.1228299695628E-6
12-13	6.1228299695628E-6	1.408250892999444E-4	0.0	1.22456599391256E-5	6.1228299695628E-6
14-15	9.1842449543442E-6	2.020533889955724E-4	0.0	1.22456599391256E-5	9.1842449543442E-6
16-17	3.0614149847814E-5	2.112376339499166E-4	0.0	1.5307074923906998E-5	1.83684899086884E-5
18-19	3.6736979817376806E-5	2.357289538281678E-4	0.0	1.83684899086884E-5	3.0614149847814E-5
20-21	4.5921224771721E-5	2.632816886912004E-4	0.0	3.0614149847813996E-5	5.51054697260652E-5
22-23	4.89826397565024E-5	3.092029134629214E-4	0.0	5.2044054741283804E-5	7.347395963475361E-5
24-25	5.2044054741283804E-5	3.245099883868284E-4	0.0	1.0408810948256761E-4	8.878103455866061E-5
26-27	6.1228299695628E-5	3.306328183563912E-4	0.0	1.714392391477584E-4	9.79652795130048E-5
28-29	6.1228299695628E-5	3.428784782955168E-4	0.0	7.163711064388476E-4	1.07149524467349E-4
30-31	6.1228299695628E-5	3.7961545811289357E-4	0.0	0.0022378943538752033	1.193951844064746E-4
32-33	6.1228299695628E-5	3.7961545811289357E-4	0.0	0.004732947566472044	1.255180143760374E-4
34-35	6.1228299695628E-5	4.5921224771721E-4	0.0	0.007919880565629481	1.285794293608188E-4
36-37	6.73511296651908E-5	5.388090373215264E-4	0.0	0.01239873068836467	1.285794293608188E-4
38-39	7.34739596347536E-5	5.479932822758706E-4	0.0	0.019733880991900904	1.285794293608188E-4
40-41	7.95967896043164E-5	6.153444119410614E-4	0.0	0.02753436637312391	1.285794293608188E-4
42-43	7.95967896043164E-5	6.27590071880187E-4	0.0	0.03448071697359291	1.285794293608188E-4
44-45	7.95967896043164E-5	7.010640315149406E-4	0.0	0.04200873642117037	1.285794293608188E-4
46-47	8.57196195738792E-5	7.745379911496942E-4	0.0	0.05001739802135852	1.347022593303816E-4
48-49	8.57196195738792E-5	7.898450660736012E-4	0.0	0.058130147731029216	1.408250892999444E-4
50-51	8.57196195738792E-5	9.03117420510513E-4	0.0	0.06644495082969551	1.408250892999444E-4
52-53	8.57196195738792E-5	9.123016654648572E-4	0.0	0.07421788347605549	1.469479192695072E-4
54-55	8.57196195738792E-5	9.214859104192014E-4	0.0	0.08094075078263543	1.5613216422385138E-4
56-57	8.57196195738792E-5	9.429158153126712E-4	0.0	0.08612066493688555	1.591935792086328E-4
58-59	9.1842449543442E-5	9.551614752517967E-4	0.0	0.0914812025752378	1.622549941934142E-4
60-61	9.490386452822339E-5	9.796527951300479E-4	0.0	0.09682031030869656	1.653164091781956E-4
62-63	1.010266944977862E-4	9.980212850387363E-4	0.0	0.10219921643695748	1.653164091781956E-4
64-65	1.040881094825676E-4	0.0011143550544604297	0.0	0.10699033088814036	1.653164091781956E-4
66-67	1.07149524467349E-4	0.0011357849593538992	0.0	0.11182736656409498	1.68377824162977E-4
68-69	1.102109394521304E-4	0.0011755833541560576	0.0	0.1165970511103844	1.714392391477584E-4
70-71	1.102109394521304E-4	0.0012031360890190903	0.0	0.12105447132822611	1.714392391477584E-4
72-73	1.102109394521304E-4	0.001209258918988653	0.0	0.12488736288917243	1.775620691173212E-4
74-75	1.102109394521304E-4	0.0012429344838212485	0.0	0.12773141741003435	1.83684899086884E-4
76-77	1.102109394521304E-4	0.0013133470284712205	0.0	0.13026933043241812	1.83684899086884E-4
78-79	1.102109394521304E-4	0.0013347769333646904	0.0	0.13209699517833262	1.83684899086884E-4
80-81	1.163337694216932E-4	0.0013347769333646904	0.0	0.13261437431076067	1.8980772905644679E-4
82-83	1.163337694216932E-4	0.0013439611783190347	0.0	0.13292051580923883	1.98991974010791E-4
84-85	1.163337694216932E-4	0.0013531454232733788	0.0	0.1330062354288127	2.020533889955724E-4
86-87	1.163337694216932E-4	0.0013806981581364114	0.0	0.13306440231352357	2.051148039803538E-4
88	1.163337694216932E-4	0.001500093342542886	0.0	0.13306746372850833	2.081762189651352E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27760	0.0	52.37672	1
GTATCAA	50555	0.0	35.806797	1
ATCAACG	66070	0.0	26.888481	3
TCAACGC	66260	0.0	26.781824	4
TATCAAC	67260	0.0	26.555866	2
CAACGCA	67240	0.0	26.382717	5
AACGCAG	69405	0.0	25.608173	6
ACGCAGA	79640	0.0	22.252125	7
CGCAGAG	80685	0.0	21.951977	8
GTACATG	67270	0.0	20.27648	1
TACATGG	67160	0.0	19.981573	2
ACATGGG	68060	0.0	19.400917	3
GCAGAGT	90420	0.0	19.244974	9
GAGTACT	50055	0.0	18.370344	12-13
AGAGTAC	83620	0.0	18.008253	10-11
CAGAGTA	88345	0.0	17.732	10-11
TGGTATC	10465	0.0	17.587635	2
GTGGTAT	11360	0.0	17.317488	1
AGTACTT	54170	0.0	17.080448	12-13
CATGGGG	40060	0.0	16.940939	4
>>END_MODULE