FastQCFastQC Report
Fri 27 May 2016
SRR2139854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15130078
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219100.051.5593531
GTATCAA398250.035.3027881
ATCAACG516000.026.7496853
TCAACGC517150.026.7156624
CAACGCA525150.026.2797135
TATCAAC533500.026.092572
AACGCAG539650.025.6088146
ACGCAGA624850.022.043617
CGCAGAG628650.021.8822068
GTACATG552250.020.9307141
TACATGG542950.020.6934432
ACATGGG551000.019.8945923
GCAGAGT716850.018.8585059
GTGGTAT94300.018.1691761
GAGTACT400800.018.13392312-13
AGAGTAC664750.017.74191310-11
CAGAGTA705100.017.29288110-11
TGGTATC90500.016.8944422
CATGGGG323950.016.8316334
AGTACTT437700.016.32450312-13
Produced by FastQC (version 0.11.5)