##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15130078
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223167058358854	31.0	31.0	34.0	28.0	34.0
2	31.304283031455622	31.0	31.0	34.0	28.0	34.0
3	31.313431166713087	31.0	31.0	34.0	28.0	34.0
4	35.010019049472184	37.0	35.0	37.0	32.0	37.0
5	34.71687350190792	37.0	35.0	37.0	31.0	37.0
6	34.78147396199808	37.0	35.0	37.0	31.0	37.0
7	34.63925162844501	37.0	35.0	37.0	30.0	37.0
8	34.622498178793265	37.0	35.0	37.0	30.0	37.0
9	36.07968577557895	39.0	35.0	39.0	30.0	39.0
10-11	36.010361546054156	39.0	35.0	39.0	30.0	39.0
12-13	35.967713451312015	39.0	35.0	39.0	30.0	39.0
14-15	37.04891101685001	39.0	36.0	41.0	30.0	41.0
16-17	37.089767713028316	39.0	36.0	41.0	30.0	41.0
18-19	37.151169775859714	39.5	36.0	41.0	30.0	41.0
20-21	37.21035595337608	40.0	36.0	41.0	30.0	41.0
22-23	37.1903940614225	40.0	36.0	41.0	30.0	41.0
24-25	37.16314162293351	39.5	36.0	41.0	30.0	41.0
26-27	37.075533683313466	39.0	36.0	41.0	30.0	41.0
28-29	37.03449857428929	39.0	36.0	41.0	30.0	41.0
30-31	36.93956051568945	39.0	36.0	41.0	30.0	41.0
32-33	36.83827236270834	39.0	35.0	41.0	30.0	41.0
34-35	36.784618748458094	39.0	35.0	41.0	30.0	41.0
36-37	36.76766575728601	39.0	35.0	41.0	30.0	41.0
38-39	36.720854298015155	39.0	35.0	41.0	30.0	41.0
40-41	36.70446696860064	39.0	35.0	41.0	30.0	41.0
42-43	36.6699753414411	39.0	35.0	41.0	30.0	41.0
44-45	36.64239792161857	39.0	35.0	41.0	30.0	41.0
46-47	36.606711587606384	39.0	35.0	41.0	30.0	41.0
48-49	36.549394552968515	39.0	35.0	41.0	29.5	41.0
50-51	36.49814033789887	39.0	35.0	40.0	29.0	41.0
52-53	36.433025553192394	39.0	35.0	40.0	29.0	41.0
54-55	36.343310567514294	38.5	34.5	40.0	29.0	41.0
56-57	36.253684203338274	38.0	34.0	40.0	29.0	41.0
58-59	36.12156982335438	38.0	34.0	40.0	28.5	41.0
60-61	35.96032488433028	38.0	34.0	40.0	28.0	41.0
62-63	35.79789674724308	38.0	34.0	40.0	28.0	41.0
64-65	35.61909739997304	37.0	34.0	40.0	28.0	41.0
66-67	35.40324087990354	37.0	34.0	40.0	28.0	41.0
68-69	35.16523842766438	36.5	33.0	39.0	28.0	41.0
70-71	34.934405454829886	36.0	33.0	39.0	28.0	41.0
72-73	34.651091323071505	36.0	33.0	39.0	28.0	40.0
74-75	34.37647400773098	35.0	33.0	38.5	28.0	40.0
76-77	34.094100577670886	35.0	32.0	37.5	28.0	39.5
78-79	33.81594325360447	35.0	32.0	37.0	27.5	39.0
80-81	33.563920485353485	35.0	32.0	36.5	27.0	39.0
82-83	33.44165507759893	35.0	32.0	36.0	27.5	38.5
84-85	33.400850370624156	35.0	32.0	36.0	28.0	37.0
86-87	33.240666531171954	35.0	32.0	35.5	28.0	37.0
88-89	33.09810553334431	35.0	32.0	35.0	28.0	36.5
90-91	33.01755965283152	35.0	32.0	35.0	28.0	36.0
92-93	32.967213524090155	35.0	32.0	35.0	29.0	36.0
94-95	32.94149538263743	34.5	32.0	35.0	29.0	36.0
96-97	32.9911586427168	34.0	32.0	35.0	29.0	35.5
98-99	33.09016558474356	34.0	32.0	35.0	29.0	35.0
100	32.74633735880403	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	13.0
15	28.0
16	67.0
17	237.0
18	1627.0
19	8191.0
20	26160.0
21	59164.0
22	100935.0
23	141585.0
24	178636.0
25	216820.0
26	252941.0
27	288132.0
28	328230.0
29	372129.0
30	428290.0
31	501241.0
32	600559.0
33	733447.0
34	928566.0
35	1204204.0
36	1661801.0
37	2467941.0
38	2973838.0
39	1595467.0
40	59824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.130130194966604	21.19953380279996	14.431538290813833	24.238797711419597
2	14.31628442364937	22.490756491803943	40.58025345275814	22.612705631788547
3	16.854680866381173	27.88034309740544	31.543812058386774	23.721163977826613
4	12.013597997225773	19.373470697951376	39.60323155702052	29.009699747802326
5	12.007876632931245	39.23779619642237	36.23124148580496	12.523085684841417
6	27.863849029494787	39.975693561392504	19.483801284185105	12.67665612492761
7	24.73556709859076	33.96452802646024	24.462784067191826	16.83712080775717
8	24.052630705774863	36.969496957006	21.873243273022496	17.10462906419664
9	25.53639851175495	16.40287455913368	21.655029767875682	36.405697161235686
10-11	23.282831606544985	27.3636839937821	29.867238157901305	19.486246241771614
12-13	24.452801947619974	25.199838385718405	29.815888471076153	20.531471195585468
14-15	21.685375842590425	26.28607490015824	27.595852716713793	24.432696540537542
16-17	20.829243355912023	29.360821418490918	28.24154318578284	21.568392039814213
18-19	20.863047522542395	28.477329370009286	30.131623868914183	20.52799923853414
20-21	21.973140761036248	27.56698634737055	29.879618277847005	20.5802546137462
22-23	21.57717993781979	27.48106763656817	29.79016505650243	21.151587369109613
24-25	21.19532710965356	27.6456915372753	29.935633722274353	21.223347630796784
26-27	21.172048693594192	27.830358833602986	29.739436839091542	21.25815563371128
28-29	21.1377958804314	27.879785072988355	29.80926873153646	21.17315031504378
30-31	21.441843786238408	27.741335957874195	29.672860700589	21.143959555298395
32-33	21.246061359735297	27.954085376435838	29.532196491171142	21.267656772657727
34-35	21.34998579523833	27.991816467743874	29.570171590018653	21.088026146999145
36-37	21.3621176371643	27.898491928625642	29.49481094831944	21.24457948589063
38-39	21.213709698498604	28.00797623925055	29.510414427616066	21.267899634634777
40-41	21.475043850729683	27.896022239896478	29.35947145575798	21.269462453615855
42-43	21.35753005983905	28.00589767859993	29.153346715907126	21.483225545653898
44-45	21.435299349288393	28.17448049417117	28.930970567099624	21.459249589440812
46-47	21.548221820190847	28.164352555207145	28.636895080126067	21.650530544475938
48-49	21.530768504623165	28.309656727402466	28.555357923233814	21.604216844740552
50-51	21.517366163340313	28.395522191584625	28.4248226832098	21.66228896186526
52-53	21.65415054901076	28.27918112619152	28.378473117784424	21.68819520701329
54-55	21.602979416927383	28.225116927679416	28.45486694068248	21.717036714710723
56-57	21.621123776145854	28.312721741701136	28.383741745222125	21.682412736930885
58-59	21.61146513206087	28.360357768224038	28.38336143025431	21.644815669460783
60-61	21.685709290993927	28.336348332349797	28.376426410039223	21.601515966617054
62-63	21.572770731790804	28.400754310635364	28.386953181545138	21.639521776028698
64-65	21.569581711436232	28.31605866669343	28.380623112681057	21.733736509189285
66-67	21.666148605507328	28.420205351959936	28.20148114224756	21.712164900285178
68-69	21.649118552790917	28.36124936916178	28.287159863330206	21.702472214717098
70-71	21.622210604360355	28.149430176627437	28.372925723788473	21.855433495223735
72-73	21.643875406209922	28.227915202696792	28.302811808895868	21.82539758219742
74-75	21.492728461238737	28.37937654983006	28.29004108332694	21.83785390560426
76-77	21.62498039444676	28.292973335768608	28.288801851171613	21.79324441861302
78-79	21.61451128177194	28.35228435090092	28.189534610767257	21.843669756559887
80-81	21.58631208966112	28.3625695216835	28.177577518307277	21.873540870348098
82-83	21.68018693557995	28.309761079659516	28.290169088286525	21.719882896474
84-85	21.67437954177371	28.318741667262444	28.23780130143953	21.769077489524317
86-87	21.528049751847888	28.357737751652156	28.325704970382137	21.78850752611782
88-89	21.629596681254494	28.3209419532001	28.25230075304056	21.797160612504847
90-91	21.654151299698576	28.324893160353547	28.333976461553807	21.68697907839407
92-93	21.65049448527483	28.332458718670605	28.28803877050559	21.72900802554897
94-95	21.485597560889495	28.409612930791234	28.343666057998227	21.761123450321037
96-97	21.50271422023463	28.54931556062089	28.44575647584951	21.50221374329497
98-99	22.451045268895246	30.491009225586353	28.647266543244854	18.41067896227355
100	21.63294833764931	31.461073373111432	22.37335200437574	24.532626284863518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4799.0
1	4627.5
2	5751.5
3	7159.0
4	8810.0
5	14327.5
6	19517.5
7	23150.5
8	25321.0
9	24114.0
10	20331.0
11	16315.5
12	16789.5
13	22733.5
14	39234.5
15	63536.5
16	83033.5
17	93450.0
18	97096.5
19	95738.0
20	90783.0
21	86154.0
22	85938.0
23	94236.0
24	112409.0
25	139595.5
26	175017.5
27	212259.0
28	247406.0
29	285632.0
30	322701.5
31	358233.5
32	398971.5
33	438426.0
34	476086.0
35	511017.5
36	546036.0
37	577865.5
38	597152.5
39	610746.5
40	625692.0
41	636422.0
42	640159.5
43	645805.0
44	649093.0
45	649659.5
46	647160.0
47	639112.5
48	630234.5
49	615748.0
50	595956.5
51	571026.0
52	538174.0
53	500138.5
54	461285.5
55	423518.5
56	386673.5
57	348627.5
58	310753.0
59	274109.0
60	234099.5
61	191117.0
62	150587.5
63	117465.5
64	91152.5
65	70174.5
66	53596.5
67	41332.5
68	31971.5
69	24252.0
70	18376.0
71	13883.5
72	10497.5
73	7920.5
74	5825.5
75	4340.0
76	3252.5
77	2384.0
78	1749.0
79	1265.5
80	947.5
81	730.5
82	567.5
83	401.5
84	287.0
85	222.0
86	181.0
87	142.0
88	84.5
89	44.0
90	30.0
91	26.5
92	21.5
93	11.0
94	3.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.021777812381403455
4	0.014950352536186528
5	0.07893548202461349
6	0.048486200798171696
7	0.010383290819782951
8	0.004857873171572546
9	0.014494307299671555
10-11	0.020512121616293056
12-13	0.0015003227346217251
14-15	0.007521441726870146
16-17	6.906772060263007E-4
18-19	0.00900854575898419
20-21	0.002014392420380478
22-23	0.0013577871460675288
24-25	0.011546477573255387
26-27	8.568111772811403E-4
28-29	0.001457413334233957
30-31	0.0045723555914936025
32-33	0.024763891759036642
34-35	3.9064625822849195E-4
36-37	8.471333042868267E-5
38-39	8.95185130605973E-4
40-41	0.003346152778091599
42-43	0.014591911030377021
44-45	9.371438919268752E-4
46-47	0.0018095724455011745
48-49	6.86669046878426E-5
50-51	0.0011189130308392177
52-53	4.54567292589814E-4
54-55	3.584463724461676E-4
56-57	1.3211250806877145E-4
58-59	0.009261695505687811
60-61	0.00694190341506108
62-63	5.157813260667559E-4
64-65	0.011178911847880843
66-67	0.0012144095606629734
68-69	0.005097790251440318
70-71	0.005129518709909259
72-73	0.02469547634249633
74-75	5.074423337918767E-4
76-77	0.00616560876051108
78-79	6.445359933820763E-5
80-81	6.096145533456496E-5
82-83	0.0
84-85	0.0
86-87	0.012698229763481766
88-89	2.1596796358798513E-4
90-91	9.802113989250347E-5
92-93	2.848649134972082E-5
94-95	1.789534233088575E-4
96-97	4.1128133808946343E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	9591.0
20-21	17596.0
22-23	19653.0
24-25	21437.0
26-27	26233.0
28-29	39435.0
30-31	60836.0
32-33	68431.0
34-35	85211.0
36-37	113213.0
38-39	139857.0
40-41	138421.0
42-43	153857.0
44-45	181477.0
46-47	177908.0
48-49	164911.0
50-51	148185.0
52-53	137834.0
54-55	142630.0
56-57	148570.0
58-59	153114.0
60-61	150404.0
62-63	139339.0
64-65	139268.0
66-67	146914.0
68-69	152818.0
70-71	154205.0
72-73	141873.0
74-75	138380.0
76-77	144677.0
78-79	151674.0
80-81	156695.0
82-83	146542.0
84-85	144668.0
86-87	152162.0
88-89	165692.0
90-91	178929.0
92-93	188384.0
94-95	217100.0
96-97	489497.0
98-99	870237.0
100-101	8812220.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.40248232413653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.58812048680073	77.36450358650748
2	6.542398279401404	11.174741068280793
3	1.5853152843722134	4.061695816553442
4	0.589132201887492	2.0125340983310465
5	0.25144542668255787	1.073703180387106
6	0.14477614351320275	0.741854522240577
7	0.07906409911079773	0.4726589228748578
8	0.05202561964022292	0.355449364938111
9	0.035547934551548054	0.2732293666978331
>10	0.12474472813031756	1.6815312680712722
>50	0.004698946881912845	0.2712972404874342
>100	0.0025676159217062526	0.40122500138231904
>500	1.3356689190753475E-4	0.07106176793398933
>1k	2.9666213992546614E-5	0.04451479531379969
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3218702507680396E-5
2	0.0	6.609351253840198E-6	0.0	0.0	1.3218702507680396E-5
3	0.0	6.609351253840198E-6	0.0	6.609351253840198E-6	1.3218702507680396E-5
4	0.0	6.609351253840198E-5	0.0	6.609351253840198E-6	1.3218702507680396E-5
5	6.609351253840198E-6	6.609351253840198E-5	0.0	6.609351253840198E-6	1.3218702507680396E-5
6	1.3218702507680396E-5	6.609351253840198E-5	0.0	6.609351253840198E-6	1.3218702507680396E-5
7	1.3218702507680396E-5	6.609351253840198E-5	0.0	1.3218702507680396E-5	1.9828053761520596E-5
8	1.3218702507680396E-5	7.931221504608238E-5	0.0	1.9828053761520596E-5	1.9828053761520596E-5
9	1.3218702507680396E-5	1.5862443009216477E-4	0.0	3.304675626920099E-5	1.9828053761520596E-5
10-11	1.3218702507680396E-5	1.5862443009216477E-4	0.0	3.304675626920099E-5	1.9828053761520596E-5
12-13	1.3218702507680396E-5	1.5862443009216477E-4	0.0	3.9656107523041185E-5	1.9828053761520596E-5
14-15	1.3218702507680396E-5	2.2471794263056674E-4	0.0	5.9484161284561785E-5	2.6437405015360792E-5
16-17	3.965610752304119E-5	2.445459963920873E-4	0.0	6.278883691148188E-5	2.6437405015360792E-5
18-19	4.6265458776881385E-5	2.742880770343682E-4	0.0	8.592156629992257E-5	3.635143189612109E-5
20-21	4.6265458776881385E-5	3.073348333035692E-4	0.0	1.2227299819604366E-4	3.965610752304119E-5
22-23	5.9484161284561785E-5	3.866470483496516E-4	0.0	1.5201507883832457E-4	3.965610752304119E-5
24-25	6.939818816532208E-5	4.196938046188526E-4	0.0	1.8836651073444565E-4	3.965610752304119E-5
26-27	7.270286379224218E-5	4.296078314996129E-4	0.0	2.7759275266128834E-4	3.965610752304119E-5
28-29	7.600753941916228E-5	4.3291250712653296E-4	0.0	7.369426648031821E-4	3.965610752304119E-5
30-31	8.261689067300248E-5	4.758732902764943E-4	0.0	0.0020885549962135026	3.965610752304119E-5
32-33	8.922624192684268E-5	4.8248264153033445E-4	0.0	0.0045736710676574175	3.965610752304119E-5
34-35	9.253091755376277E-5	5.551855053225767E-4	0.0	0.007237239622955017	3.965610752304119E-5
36-37	9.253091755376277E-5	6.34497720368659E-4	0.0	0.011530013262324227	3.965610752304119E-5
38-39	9.253091755376277E-5	6.642398010109399E-4	0.0	0.0186780266433524	4.6265458776881385E-5
40-41	9.253091755376277E-5	7.00591232907061E-4	0.0	0.02657289671606452	4.6265458776881385E-5
42-43	9.253091755376277E-5	7.270286379224218E-4	0.0	0.03331443499498152	4.6265458776881385E-5
44-45	9.583559318068287E-5	8.228642311031047E-4	0.0	0.040531846564175016	5.2874810030721585E-5
46-47	9.914026880760297E-5	8.922624192684267E-4	0.0	0.04855229431071009	5.6179485657641685E-5
48-49	9.914026880760297E-5	9.054811217761071E-4	0.0	0.056536390625349056	5.9484161284561785E-5
50-51	1.1896832256912357E-4	0.001067410227495192	0.0	0.06472868150448399	6.609351253840198E-5
52-53	1.3218702507680397E-4	0.0010839336056297924	0.0	0.07217411569193496	6.609351253840198E-5
54-55	1.3218702507680397E-4	0.0010839336056297924	0.0	0.07948075350305531	6.609351253840198E-5
56-57	1.3218702507680397E-4	0.001097152308137473	0.0	0.08592817565117641	6.609351253840198E-5
58-59	1.3218702507680397E-4	0.001097152308137473	0.0	0.09185345905024415	6.609351253840198E-5
60-61	1.3879637633064417E-4	0.001107066335018233	0.0	0.0981918269026769	6.609351253840198E-5
62-63	1.5862443009216477E-4	0.0011136756862720734	0.0	0.104226164597433	6.609351253840198E-5
64-65	1.5862443009216477E-4	0.0011896832256912356	0.0	0.10956321573490896	6.609351253840198E-5
66-67	1.5862443009216477E-4	0.0012095112794527564	0.0	0.11489365752113109	6.609351253840198E-5
68-69	1.5862443009216477E-4	0.0012590814138565578	0.0	0.12062726973383746	6.609351253840198E-5
70-71	1.7184313259984517E-4	0.0012888234944988387	0.0	0.12583874319749044	6.609351253840198E-5
72-73	1.8506183510752554E-4	0.0012987375213795991	0.0	0.13001254851429056	7.270286379224218E-5
74-75	1.8506183510752554E-4	0.00133508895327572	0.0	0.13320156049426843	7.270286379224218E-5
76-77	1.8506183510752554E-4	0.0013813544120526015	0.0	0.13618568258537728	7.270286379224218E-5
78-79	1.8506183510752554E-4	0.0013879637633064416	0.0	0.13807595704397557	7.270286379224218E-5
80-81	1.8506183510752554E-4	0.0013879637633064416	0.0	0.13879968100627108	7.600753941916228E-5
82-83	1.8506183510752554E-4	0.0013912684389333616	0.0	0.13918963273024765	7.931221504608238E-5
84-85	1.8506183510752554E-4	0.001394573114560282	0.0	0.13929207767468216	7.931221504608238E-5
86-87	1.8506183510752554E-4	0.0014210105195756428	0.0	0.13933503845783213	8.592156629992257E-5
88	1.8506183510752554E-4	0.0015003227346217251	0.0	0.13933834313345905	9.253091755376277E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21910	0.0	51.559353	1
GTATCAA	39825	0.0	35.302788	1
ATCAACG	51600	0.0	26.749685	3
TCAACGC	51715	0.0	26.715662	4
CAACGCA	52515	0.0	26.279713	5
TATCAAC	53350	0.0	26.09257	2
AACGCAG	53965	0.0	25.608814	6
ACGCAGA	62485	0.0	22.04361	7
CGCAGAG	62865	0.0	21.882206	8
GTACATG	55225	0.0	20.930714	1
TACATGG	54295	0.0	20.693443	2
ACATGGG	55100	0.0	19.894592	3
GCAGAGT	71685	0.0	18.858505	9
GTGGTAT	9430	0.0	18.169176	1
GAGTACT	40080	0.0	18.133923	12-13
AGAGTAC	66475	0.0	17.741913	10-11
CAGAGTA	70510	0.0	17.292881	10-11
TGGTATC	9050	0.0	16.894442	2
CATGGGG	32395	0.0	16.831633	4
AGTACTT	43770	0.0	16.324503	12-13
>>END_MODULE