FastQCFastQC Report
Fri 27 May 2016
SRR2139853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10510324
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125950.050.0364231
GTATCAA238300.036.4582251
TCAACGC302650.028.1888374
ATCAACG304200.028.1263393
CAACGCA309300.027.5561755
TATCAAC319250.027.033372
AACGCAG317750.026.849266
ACGCAGA369500.023.055517
CGCAGAG370400.022.9883868
GTACATG326500.019.9571081
TACATGG323900.019.6219962
GCAGAGT433100.019.4703949
ACATGGG326050.018.9619883
AGAGTAC401250.018.1710610-11
GAGTACT252650.017.86065912-13
CATGGGG224400.017.157824
CAGAGTA428100.017.00256510-11
AGTACTT281900.016.01472912-13
TATACCG8100.015.7429375
GTACTTT291250.015.53702514-15
Produced by FastQC (version 0.11.5)