##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10510324
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810243433028326	31.0	31.0	34.0	30.0	34.0
2	31.971059503018175	31.0	31.0	34.0	30.0	34.0
3	31.992753696270448	31.0	31.0	34.0	30.0	34.0
4	35.7153220014911	37.0	35.0	37.0	33.0	37.0
5	35.5267808109436	37.0	35.0	37.0	33.0	37.0
6	35.533460528904726	37.0	35.0	37.0	33.0	37.0
7	35.42693916952513	37.0	35.0	37.0	33.0	37.0
8	35.40686281412447	37.0	35.0	37.0	33.0	37.0
9	36.93782189778355	39.0	37.0	39.0	33.0	39.0
10-11	36.82884457225106	39.0	37.0	39.0	32.5	39.0
12-13	36.77049094775765	39.0	37.0	39.0	32.0	39.0
14-15	37.87304130681414	40.0	37.0	41.0	33.0	41.0
16-17	37.85822896610989	40.0	37.0	41.0	32.5	41.0
18-19	37.902101733495556	40.0	37.0	41.0	33.0	41.0
20-21	37.88491752080408	40.0	37.0	41.0	32.0	41.0
22-23	37.818099964716936	40.0	37.0	41.0	32.0	41.0
24-25	37.72808135119689	39.5	37.0	41.0	32.0	41.0
26-27	37.53343231160792	39.0	36.0	41.0	32.0	41.0
28-29	37.425065649355965	39.0	36.0	41.0	32.0	41.0
30-31	37.27446949047348	39.0	36.0	40.5	31.0	41.0
32-33	37.43302872816481	39.0	36.0	41.0	32.0	41.0
34-35	37.386913013784515	39.0	36.0	41.0	31.5	41.0
36-37	37.297719383471886	39.0	36.0	41.0	31.0	41.0
38-39	37.19056840326316	39.0	36.0	41.0	31.0	41.0
40-41	37.098835069319804	39.0	36.0	40.5	31.0	41.0
42-43	37.05747635655979	39.0	35.5	40.0	31.0	41.0
44-45	37.08152309006311	39.0	35.5	40.0	31.0	41.0
46-47	37.191991337181314	39.0	36.0	41.0	31.0	41.0
48-49	37.138790916884076	39.0	36.0	41.0	31.0	41.0
50-51	37.03415475922702	39.0	35.0	41.0	31.0	41.0
52-53	36.93966018301725	39.0	35.0	40.0	31.0	41.0
54-55	36.82131974242577	39.0	35.0	40.0	30.0	41.0
56-57	36.698993303736124	39.0	35.0	40.0	30.0	41.0
58-59	36.56050853894256	38.0	35.0	40.0	30.0	41.0
60-61	36.350126187267016	38.0	35.0	40.0	30.0	41.0
62-63	36.15023571128714	38.0	34.0	40.0	29.0	41.0
64-65	35.95748795964059	38.0	34.0	40.0	29.0	41.0
66-67	35.71773723069379	37.0	34.0	40.0	29.0	41.0
68-69	35.45310280937596	37.0	34.0	39.5	29.0	41.0
70-71	35.168323997880755	36.0	34.0	39.0	29.0	40.5
72-73	34.877640480065764	36.0	33.0	39.0	28.0	40.0
74-75	34.52920253487045	35.5	33.0	38.5	28.0	40.0
76-77	33.47571725927661	34.5	31.5	37.0	26.5	39.0
78-79	33.89571957271462	35.0	32.5	37.0	28.0	39.0
80-81	33.79009292416949	35.0	33.0	37.0	28.0	39.0
82-83	33.54984768437048	35.0	32.5	36.0	28.0	38.5
84-85	33.311922039512666	35.0	32.0	36.0	28.0	37.0
86-87	33.09240528854751	35.0	32.0	35.5	28.0	37.0
88-89	32.8723435214836	34.0	32.0	35.0	27.0	36.5
90-91	32.73574426618447	34.0	32.0	35.0	27.0	36.0
92-93	32.65383443726101	34.0	32.0	35.0	27.0	36.0
94-95	32.61047728843121	34.0	32.0	35.0	27.0	36.0
96-97	32.61935506615221	34.0	32.0	35.0	27.5	35.0
98-99	32.73483598905824	34.0	32.0	35.0	29.0	35.0
100-101	32.07682086960109	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	22.0
20	97.0
21	643.0
22	2793.0
23	8979.0
24	22979.0
25	47399.0
26	83846.0
27	129602.0
28	184038.0
29	241635.0
30	305495.0
31	376262.0
32	461364.0
33	573445.0
34	732753.0
35	955529.0
36	1325122.0
37	1907950.0
38	2105304.0
39	1010865.0
40	34200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.43765653656348	22.978397240655948	15.686576360538456	23.897369862242115
2	13.461219714513037	23.505071693342146	41.4949159373703	21.538792654774515
3	16.22809154123127	28.897168155805662	32.33474058459092	22.539999718372144
4	11.615750380292749	20.14464064095455	40.58147018112857	27.658138797624126
5	11.81807862956802	39.204026213852075	36.80886625632081	12.169028900259088
6	27.152892717674547	40.797648102950966	19.70267519821463	12.346783981159858
7	23.843508535036598	33.97711621449538	25.494570861944883	16.684804388523133
8	23.108383718713142	37.89294221567289	22.456615038699095	16.54205902691487
9	25.037439378652838	16.63027704949914	22.41982264295563	35.91246092889239
10-11	22.91251440012696	27.74315996347972	30.469698174861215	18.87462746153211
12-13	23.87153811813984	25.56958282161425	30.329340941344913	20.229538118900997
14-15	21.134824197617505	26.83131842557851	27.972163370035023	24.061694006768963
16-17	20.467661130142133	29.772207783508865	28.644730647694587	21.115400438654415
18-19	20.493319474532026	29.01306182032967	30.396173235396915	20.097445469741388
20-21	21.410179856801356	28.191827019439675	30.13014256922425	20.26785055453472
22-23	21.14006444292669	28.07789963907279	29.99055120028496	20.791484717715562
24-25	20.86999528694921	28.342403568324908	30.00637418462841	20.78122696009747
26-27	20.82695466362389	28.46427127438939	29.874009268338447	20.83476479364827
28-29	20.79662602845577	28.491236373187395	29.927719806391284	20.784417791965552
30-31	21.10026074001385	28.373810273197574	29.751403616772343	20.77452537001623
32-33	20.88080331689544	28.582943081957456	29.691970778819165	20.844282822327937
34-35	21.0293115626787	28.635141631976683	29.69519091532365	20.640355890020967
36-37	20.972378156715983	28.541329341109577	29.694321244716264	20.791971257458176
38-39	20.82212065019176	28.656188787323355	29.68746011707629	20.834230445408593
40-41	21.029432398610886	28.494302684058365	29.61452229955242	20.86174261777833
42-43	20.930936014810523	28.657325490903148	29.313958932665447	21.097779561620886
44-45	21.036959805435185	28.76983744038222	29.11838394176531	21.07481881241729
46-47	21.095214452590763	28.690916770483998	28.943804618322822	21.27006415860242
48-49	20.994573566389345	28.878062967107958	28.898854589764838	21.228508876737852
50-51	21.046204195273095	28.962790146817817	28.69130138979742	21.299704268111668
52-53	21.16088067860905	28.834779501971568	28.59682499651921	21.407514822900165
54-55	21.120195584364332	28.77591640897664	28.665442194590224	21.4384458120688
56-57	21.130639429319125	28.84859210749027	28.609282526338113	21.411485936852497
58-59	21.108673728812636	28.881356642715666	28.618649330373287	21.391320298098407
60-61	21.240095829265265	28.838534988968593	28.62310771314312	21.29826146862302
62-63	21.154691436798352	28.91999262896146	28.591695430111898	21.33362050412829
64-65	21.099579935894052	28.903627670712684	28.545935883576778	21.45085650981649
66-67	21.213206088600096	29.021951525937318	28.337498596531862	21.427343788930724
68-69	21.18642413963455	28.970285483511283	28.405629350861446	21.437661025992725
70-71	21.18267928187159	28.788553711020327	28.4681640713276	21.560602935780484
72-73	21.21197787662745	28.759877304455838	28.47162926500478	21.556515553911925
74-75	21.164369565685824	28.807811975301856	28.43740761983667	21.590410839175654
76-77	21.284068173638836	28.785243748564703	28.346401352276708	21.58428672551975
78-79	21.275840704491188	28.78172642996968	28.312684232146278	21.629748633392857
80-81	21.29708254257407	28.787235495469698	28.31390324863705	21.601778713319177
82-83	21.314588141950143	28.705906163784345	28.45357466608037	21.525931028185145
84-85	21.424997669807944	28.68075265301972	28.37260240643975	21.521647270732583
86-87	21.223466630513023	28.752951271932435	28.463849129127013	21.559732968427532
88-89	21.319816373635014	28.664359528415282	28.469185424809005	21.54663867314069
90-91	21.39061903166249	28.60674103059413	28.56238033760456	21.440259600138823
92-93	21.430874950991075	28.529885893774477	28.55267313232328	21.486566022911173
94-95	21.242790393520664	28.54521447216617	28.674702643154138	21.53729249115903
96-97	21.121545554003035	28.559822012565704	28.845863576810103	21.47276885662116
98-99	21.214891514840136	29.215768881160336	28.873912184325967	20.695427419673557
100-101	24.678933521371064	36.26000656258192	29.924986552002757	9.136073364044261
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3776.0
1	3199.0
2	3886.0
3	5411.0
4	6967.5
5	10379.5
6	13392.5
7	16156.5
8	18120.5
9	18181.0
10	16874.0
11	15479.5
12	16879.0
13	22086.0
14	34319.0
15	50923.0
16	63908.5
17	70273.0
18	71396.5
19	69881.0
20	67832.5
21	67311.0
22	69977.5
23	78109.5
24	93162.5
25	115705.5
26	143458.5
27	171489.0
28	199708.5
29	228557.5
30	255700.5
31	282183.0
32	309502.5
33	335936.0
34	360307.5
35	382906.5
36	405925.0
37	424574.0
38	435057.0
39	441248.0
40	445090.5
41	444872.0
42	441068.0
43	439711.0
44	437919.5
45	433726.5
46	425536.5
47	413616.5
48	401405.5
49	384585.5
50	367584.0
51	349103.5
52	325495.0
53	302274.5
54	280861.5
55	259222.0
56	236721.5
57	212244.0
58	187363.0
59	162888.0
60	136737.5
61	110494.5
62	86270.0
63	66096.5
64	50018.0
65	38048.0
66	29021.5
67	22022.5
68	16783.5
69	12063.0
70	8316.0
71	6038.0
72	4308.0
73	2990.5
74	2020.5
75	1361.0
76	952.5
77	659.0
78	442.5
79	295.0
80	215.0
81	172.0
82	139.5
83	106.0
84	79.5
85	62.0
86	50.0
87	38.5
88	26.5
89	16.5
90	12.0
91	8.5
92	6.0
93	5.0
94	4.0
95	3.0
96	3.0
97	2.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004015099819948462
3	0.0
4	0.0
5	2.8543363648922716E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.3776313651225217E-4
20-21	7.183810982229631E-4
22-23	2.4266674190921725E-4
24-25	1.4279067040029366E-4
26-27	0.002633468392640899
28-29	4.765119402217677E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.033292347977747126
38-39	0.016978108954262452
40-41	0.021444835738747087
42-43	0.010878408154700682
44-45	0.004175746283059454
46-47	0.0051474500781102705
48-49	0.009302678899124063
50-51	0.006909048998258077
52-53	0.011342139715933773
54-55	0.003672432208334319
56-57	0.016794411506650803
58-59	0.01331278574711006
60-61	0.012994867961642271
62-63	0.005062881545151389
64-65	0.006640065105303322
66-67	4.395183381320745E-4
68-69	7.643889709094984E-4
70-71	0.0012930145535253073
72-73	8.947163421132842E-5
74-75	0.0036799392228985188
76-77	0.004687335378846356
78-79	0.003743860770310585
80-81	6.854046133822966E-4
82-83	0.0033321913904827832
84-85	0.004564931786782611
86-87	0.001457355732311562
88-89	0.07805367721741605
90-91	0.001085180023366152
92-93	1.422868267654327E-5
94-95	6.052175439878308E-4
96-97	0.012114348622528628
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	358.0
20-21	1255.0
22-23	2361.0
24-25	5304.0
26-27	6300.0
28-29	8278.0
30-31	29056.0
32-33	37126.0
34-35	52938.0
36-37	78487.0
38-39	86843.0
40-41	92879.0
42-43	105506.0
44-45	122163.0
46-47	124217.0
48-49	113135.0
50-51	103377.0
52-53	94115.0
54-55	101399.0
56-57	107909.0
58-59	112907.0
60-61	111064.0
62-63	107226.0
64-65	115717.0
66-67	124589.0
68-69	126787.0
70-71	126247.0
72-73	121557.0
74-75	122131.0
76-77	123063.0
78-79	132855.0
80-81	137330.0
82-83	131391.0
84-85	131159.0
86-87	139357.0
88-89	147178.0
90-91	158837.0
92-93	165351.0
94-95	199384.0
96-97	252374.0
98-99	820147.0
100-101	5630667.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.06816647235041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82381523804968	78.17039249562457
2	6.47160234114151	11.139978952804402
3	1.4685430489311282	3.791844228216522
4	0.5357124486056855	1.8443115283161842
5	0.2531205499627728	1.089281081588441
6	0.14299455420329457	0.7384367457485216
7	0.08240934678489596	0.4964974964572058
8	0.05301852992821474	0.3650566127984703
9	0.03425648482541584	0.2653553554840293
>10	0.1305596693325545	1.7786582478643904
>50	0.003188365178822795	0.18782432131512505
>100	7.24853387258937E-4	0.10022231660194111
>500	5.456966874204049E-5	0.032140617180200205
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.514454549640905E-6	1.902890909928181E-5
2	0.0	1.902890909928181E-5	0.0	1.902890909928181E-5	2.8543363648922716E-5
3	0.0	1.902890909928181E-5	0.0	1.902890909928181E-5	3.805781819856362E-5
4	0.0	8.563009094676815E-5	0.0	1.902890909928181E-5	3.805781819856362E-5
5	0.0	9.514454549640905E-5	0.0	2.8543363648922716E-5	3.805781819856362E-5
6	0.0	9.514454549640905E-5	0.0	2.8543363648922716E-5	4.7572272748204526E-5
7	0.0	9.514454549640905E-5	0.0	2.8543363648922716E-5	4.7572272748204526E-5
8	0.0	1.0465900004604996E-4	0.0	2.8543363648922716E-5	4.7572272748204526E-5
9	0.0	1.5223127279425448E-4	0.0	9.514454549640905E-5	5.708672729784543E-5
10-11	1.4271681824461358E-5	1.5223127279425448E-4	0.0	1.1417345459569086E-4	5.708672729784543E-5
12-13	1.902890909928181E-5	1.5223127279425448E-4	0.0	1.1417345459569086E-4	5.708672729784543E-5
14-15	1.902890909928181E-5	1.9504631826763858E-4	0.0	1.6174572734389538E-4	8.087286367194769E-5
16-17	2.3786136374102263E-5	1.9980354554245902E-4	0.0	2.235896819165613E-4	9.514454549640905E-5
18-19	2.8543363648922716E-5	2.1407522736692036E-4	0.0	3.234914546877908E-4	1.1417345459569086E-4
20-21	2.8543363648922716E-5	2.52133045565484E-4	0.0	4.3766490928348167E-4	1.2368790914533178E-4
22-23	2.8543363648922716E-5	2.7591918193958624E-4	0.0	7.04069636673427E-4	1.2368790914533178E-4
24-25	4.7572272748204526E-5	3.2349145468779075E-4	0.0	0.0015270699552173653	1.2368790914533178E-4
26-27	4.7572272748204526E-5	3.425203637870726E-4	0.0	0.0037011228198103123	1.2368790914533178E-4
28-29	4.7572272748204526E-5	3.425203637870726E-4	0.0	0.019485602917664574	1.2368790914533178E-4
30-31	5.708672729784543E-5	3.853354092604567E-4	0.0	0.053637737523600605	1.2368790914533178E-4
32-33	5.708672729784543E-5	3.900926365352771E-4	0.0	0.07021191734907506	1.2368790914533178E-4
34-35	5.708672729784543E-5	4.471793638331225E-4	0.0	0.09222836517694412	1.2368790914533178E-4
36-37	5.708672729784543E-5	5.04266091130968E-4	0.0	0.11995824296187253	1.3320236369497267E-4
38-39	6.660118184748634E-5	5.185377729554294E-4	0.0	0.16577509884566832	1.3320236369497267E-4
40-41	6.660118184748634E-5	6.089250911770179E-4	0.0	0.23804689560474063	1.3320236369497267E-4
42-43	6.660118184748634E-5	6.184395457266589E-4	0.0	0.2752721990302107	1.3795959096979312E-4
44-45	8.563009094676815E-5	6.707690457496839E-4	0.0	0.3248472644611146	1.427168182446136E-4
46-47	8.563009094676815E-5	7.135840912230679E-4	0.0	0.37937936071238143	1.427168182446136E-4
48-49	8.563009094676815E-5	7.183413184978884E-4	0.0	0.42151412268546623	1.617457273438954E-4
50-51	8.563009094676815E-5	7.801852730705543E-4	0.0	0.46056144415719247	1.617457273438954E-4
52-53	1.0465900004604997E-4	7.896997276201952E-4	0.0	0.5074296472687236	1.8553186371799766E-4
54-55	1.2368790914533178E-4	7.896997276201952E-4	0.0	0.5916991712148931	2.0931800009209992E-4
56-57	1.2844513642015223E-4	8.087286367194769E-4	0.0	0.6750743364333964	2.0931800009209992E-4
58-59	1.427168182446136E-4	8.087286367194769E-4	0.0	0.7427078365995188	2.0931800009209992E-4
60-61	1.5223127279425448E-4	8.230003185439384E-4	0.0	0.7851327894363676	2.1407522736692036E-4
62-63	1.5223127279425448E-4	8.277575458187588E-4	0.0	0.820597918770154	2.2834690919138173E-4
64-65	1.5223127279425448E-4	9.514454549640906E-4	0.0	0.8365108440044284	2.2834690919138173E-4
66-67	1.5698850006907496E-4	9.514454549640906E-4	0.0	0.8455210324629383	2.331041364662022E-4
68-69	1.617457273438954E-4	9.657171367885519E-4	0.0	0.8537938506938512	2.426185910158431E-4
70-71	1.6650295461871585E-4	9.704743640633724E-4	0.0	0.8616147323336559	2.5689027284030446E-4
72-73	1.807746364431772E-4	9.752315913381928E-4	0.0	0.8682748505184046	2.5689027284030446E-4
74-75	1.807746364431772E-4	0.0010180466368115769	0.0	0.874278471339228	2.5689027284030446E-4
76-77	1.902890909928181E-4	0.0010894050459338838	0.0	0.8795685080688284	2.5689027284030446E-4
78-79	1.902890909928181E-4	0.0011227056368576268	0.0	0.8842353480254272	2.5689027284030446E-4
80-81	1.902890909928181E-4	0.0011227056368576268	0.0	0.8866187188901122	2.5689027284030446E-4
82-83	1.902890909928181E-4	0.0011227056368576268	0.0	0.8881505460726045	2.6640472738994535E-4
84-85	1.9504631826763858E-4	0.0011227056368576268	0.0	0.8894968413913786	2.6640472738994535E-4
86-87	1.9980354554245902E-4	0.0011369773186820883	0.0	0.8900058647097844	2.6640472738994535E-4
88-89	1.9980354554245902E-4	0.0011797923641554723	0.0	0.8900772231189067	2.6640472738994535E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12595	0.0	50.036423	1
GTATCAA	23830	0.0	36.458225	1
TCAACGC	30265	0.0	28.188837	4
ATCAACG	30420	0.0	28.126339	3
CAACGCA	30930	0.0	27.556175	5
TATCAAC	31925	0.0	27.03337	2
AACGCAG	31775	0.0	26.84926	6
ACGCAGA	36950	0.0	23.05551	7
CGCAGAG	37040	0.0	22.988386	8
GTACATG	32650	0.0	19.957108	1
TACATGG	32390	0.0	19.621996	2
GCAGAGT	43310	0.0	19.470394	9
ACATGGG	32605	0.0	18.961988	3
AGAGTAC	40125	0.0	18.17106	10-11
GAGTACT	25265	0.0	17.860659	12-13
CATGGGG	22440	0.0	17.15782	4
CAGAGTA	42810	0.0	17.002565	10-11
AGTACTT	28190	0.0	16.014729	12-13
TATACCG	810	0.0	15.742937	5
GTACTTT	29125	0.0	15.537025	14-15
>>END_MODULE