FastQCFastQC Report
Fri 27 May 2016
SRR2139852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14696996
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216750.053.375761
GTATCAA385850.034.2914771
TCAACGC491500.026.4220984
ATCAACG492000.026.377493
CAACGCA500450.025.9110035
TATCAAC513650.025.3918572
AACGCAG515550.025.2072816
ACGCAGA594300.021.8234167
CGCAGAG602250.021.4921538
GTACATG501500.019.9274981
TACATGG490600.019.8251362
ACATGGG495300.019.147433
GCAGAGT683250.018.6547349
GAGTACT403950.018.21619412-13
AGAGTAC629450.018.03511410-11
CAGAGTA671350.017.82096910-11
GTGGTAT90600.016.8439451
TGGTATC83600.016.4300122
CATGGGG300500.016.1992034
AGTACTT442150.016.14852112-13
Produced by FastQC (version 0.11.5)