FastQCFastQC Report
Fri 27 May 2016
SRR2139851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25750209
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA393000.054.14461
GTATCAA738250.036.8658751
TCAACGC923350.029.037824
ATCAACG922050.029.0290553
CAACGCA938850.028.5007275
TATCAAC946450.028.4699732
AACGCAG968800.027.7033236
ACGCAGA1127750.023.6865317
CGCAGAG1137500.023.472538
GCAGAGT1297750.020.4233869
GAGTACT740000.019.29441512-13
GTACATG945800.019.1501481
TACATGG934650.018.9640162
GTGGTAT155350.018.2917371
CAGAGTA1285400.018.29059810-11
ACATGGG943650.018.2886223
AGAGTAC1203800.017.87654110-11
GTACTTT810750.017.56526214-15
TGGTATC147400.017.5539932
AGTACTT805650.016.712512-13
Produced by FastQC (version 0.11.5)