##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139851_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25750209 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.745098767936213 31.0 30.0 33.0 28.0 34.0 2 31.21828498557041 31.0 31.0 34.0 28.0 34.0 3 31.38166342649879 31.0 31.0 34.0 28.0 34.0 4 35.066427422006555 37.0 35.0 37.0 32.0 37.0 5 35.12103400791815 37.0 35.0 37.0 32.0 37.0 6 34.97733113544826 37.0 35.0 37.0 32.0 37.0 7 34.99498314751543 37.0 35.0 37.0 32.0 37.0 8 35.097008610687396 37.0 35.0 37.0 32.0 37.0 9 36.79960710221808 39.0 37.0 39.0 32.0 39.0 10-11 36.78313096410208 39.0 37.0 39.0 32.0 39.0 12-13 36.70012375433535 39.0 37.0 39.0 32.0 39.0 14-15 37.85545394990774 40.0 37.0 41.0 32.0 41.0 16-17 37.897587743850934 40.0 37.0 41.0 32.5 41.0 18-19 37.953596745564276 40.0 37.0 41.0 32.0 41.0 20-21 37.965701737544165 40.0 37.0 41.0 32.0 41.0 22-23 37.91354934792055 40.0 37.0 41.0 32.0 41.0 24-25 37.93900982537507 40.0 37.0 41.0 32.0 41.0 26-27 37.82131791686031 40.0 37.0 41.0 32.0 41.0 28-29 37.77765989810803 40.0 37.0 41.0 32.0 41.0 30-31 37.70115770664397 40.0 37.0 41.0 32.0 41.0 32-33 37.611275767292454 40.0 37.0 41.0 32.0 41.0 34-35 37.530063169939204 40.0 36.0 41.0 32.0 41.0 36-37 37.43346143829856 40.0 36.0 41.0 31.0 41.0 38-39 37.41012919799212 40.0 36.0 41.0 31.0 41.0 40-41 37.33931962386093 39.5 36.0 41.0 31.0 41.0 42-43 37.29296754561242 39.0 36.0 41.0 31.0 41.0 44-45 37.229790996023965 39.0 36.0 41.0 31.0 41.0 46-47 37.106755881852884 39.0 36.0 41.0 30.5 41.0 48-49 37.04256004369162 39.0 36.0 41.0 30.0 41.0 50-51 36.981483272898714 39.0 35.5 41.0 30.0 41.0 52-53 36.90112551804028 39.0 35.0 41.0 30.0 41.0 54-55 36.761214909013304 39.0 35.0 40.5 30.0 41.0 56-57 36.617392508447345 39.0 35.0 40.0 30.0 41.0 58-59 36.444804790859536 38.0 35.0 40.0 30.0 41.0 60-61 36.28385926245743 38.0 34.5 40.0 29.0 41.0 62-63 36.11175531328301 38.0 34.0 40.0 29.0 41.0 64-65 35.943912279775496 38.0 34.0 40.0 29.0 41.0 66-67 35.69825856336385 37.0 34.0 40.0 29.0 41.0 68-69 35.40580465137268 37.0 34.0 39.0 28.5 41.0 70-71 35.124784727799906 36.0 34.0 39.0 28.0 41.0 72-73 34.8569793288418 36.0 33.0 39.0 28.0 40.0 74-75 34.55063049129845 35.5 33.0 38.5 28.0 40.0 76-77 34.245462041660204 35.0 33.0 37.5 28.0 39.5 78-79 34.205136788611895 35.0 33.0 37.0 29.0 39.0 80-81 33.95146189597537 35.0 33.0 37.0 29.0 39.0 82-83 33.73887427539712 35.0 33.0 36.0 29.0 38.5 84-85 33.51523285204555 35.0 33.0 36.0 29.0 37.0 86-87 33.30289801824314 35.0 33.0 35.5 29.0 37.0 88-89 33.14802397360805 35.0 32.0 35.0 29.0 36.5 90-91 33.03291105130755 35.0 32.0 35.0 29.0 36.0 92-93 32.971633544990766 35.0 32.0 35.0 29.0 36.0 94-95 32.93337399853846 34.0 32.0 35.0 29.0 36.0 96-97 32.98234507274384 34.0 32.0 35.0 29.0 35.5 98-99 33.10158708820049 34.0 32.0 35.0 29.0 35.0 100 32.65918755721624 34.0 32.0 35.0 27.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 2.0 16 11.0 17 283.0 18 2747.0 19 10167.0 20 21097.0 21 36473.0 22 58875.0 23 91128.0 24 136987.0 25 196880.0 26 270625.0 27 355773.0 28 447084.0 29 548512.0 30 663300.0 31 808180.0 32 992168.0 33 1248918.0 34 1631218.0 35 2176018.0 36 3100029.0 37 4632357.0 38 5468841.0 39 2752542.0 40 99993.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1870559963222 19.49572137453331 9.773536207026513 29.543686422117972 2 13.762016455866435 23.50563834258588 37.27913431692924 25.45321088461845 3 17.019865741672234 28.11521646290327 29.4372601014617 25.427657693962797 4 11.6975633090978 20.16846154530241 38.6322767322005 29.501698413399286 5 12.579740960059574 39.506134380712346 34.8899772122572 13.024147446970877 6 27.424366079260494 40.96534152620383 18.720747400068806 12.889544994466876 7 24.752074827819843 35.10920629809257 23.18286814681776 16.955850727269826 8 24.326843327757068 37.49331510280169 20.966513320338485 17.213328249102755 9 25.82281953517348 17.288298514392643 21.239377125055565 35.64950482537831 10-11 23.119486136986307 28.080638102782 29.11147245445658 19.68840330577511 12-13 24.321291916504446 25.942272934561423 29.292690401075966 20.443744747858165 14-15 21.710134469199843 26.803460119488737 27.32298017464635 24.16342523666507 16-17 20.691004876892453 29.79192324225407 28.032541405780435 21.484530475073036 18-19 20.671436103683664 28.905254710748174 29.959450037861828 20.463859147706334 20-21 21.75573041111356 27.945797583245565 29.814105436665873 20.484366568975005 22-23 21.368436217759957 27.84676649532879 29.726689252405457 21.05810803450579 24-25 21.0185125367306 27.99235512581324 29.871620435722168 21.117511901733987 26-27 21.004682565347224 28.1732484090335 29.699551597296026 21.12251742832325 28-29 21.023056310104376 28.134361753179626 29.780416373038264 21.06216556367773 30-31 21.321000676534542 27.99070415334313 29.6945560973091 20.993739072813227 32-33 21.07183246506326 28.15506591972625 29.69428916862829 21.078812446582198 34-35 21.22324150591001 28.174712498824693 29.7041985681676 20.897847427097695 36-37 21.207055737219 28.076253545709477 29.644708212919674 21.07198250415185 38-39 21.06371620632101 28.250591631508037 29.65835399556156 21.02733816660939 40-41 21.329925287736252 28.07654667977352 29.53195337207356 21.061574660416664 42-43 21.16171876625149 28.16916337529547 29.3223351125519 21.34678274590114 44-45 21.24934516984849 28.39844483688685 29.103045049549593 21.249164943715073 46-47 21.37538948865044 28.39703551877924 28.754377215127352 21.473197777442966 48-49 21.309348999751883 28.539282384407166 28.67209542146173 21.47927319437923 50-51 21.355935090376494 28.678690652041034 28.455504607486365 21.50986965009611 52-53 21.4854094324423 28.646875972505892 28.245736613323334 21.621977981728474 54-55 21.413618087785295 28.51278867776752 28.41903065339203 21.654562581055156 56-57 21.39089997114586 28.594622933658933 28.384313545345346 21.63016354984986 58-59 21.40723601468929 28.63841578304388 28.34030843502019 21.61403976724664 60-61 21.51401566485428 28.619643921466935 28.350769987318603 21.515570426360185 62-63 21.42684758875898 28.715705995731188 28.36225195418699 21.495194461322843 64-65 21.355769752673194 28.64437498869208 28.271869515659205 21.72798574297552 66-67 21.408354463520183 28.83389925452838 28.08246038761052 21.675285894340917 68-69 21.39923072929398 28.751377161957205 28.161564770077003 21.687827338671816 70-71 21.418507746582286 28.49772401468774 28.230723411941405 21.85304482678857 72-73 21.452160006562558 28.53339225521846 28.166882788134206 21.847564950084777 74-75 21.375127306435825 28.730438583716705 28.132067886385272 21.762366223462195 76-77 21.521762210810007 28.65617467725055 28.04080789609214 21.7812552158473 78-79 21.481822087822902 28.68561609154956 28.034104715801973 21.798457104825566 80-81 21.507409322186703 28.714776875662867 27.978189176553066 21.799624625597367 82-83 21.5464857186777 28.689394925563523 28.103915779252 21.66020357650678 84-85 21.58983410282066 28.728197041784043 28.03306113644955 21.648907718945743 86-87 21.391039307948372 28.84180399238185 28.16334754265146 21.60380915701832 88-89 21.481564590075244 28.783291808173665 28.060252831277467 21.674890770473628 90-91 21.563863939937306 28.738910609816937 28.123112770861052 21.5741126793847 92-93 21.586017826036674 28.759126041678616 28.116379718745087 21.538476413539616 94-95 21.424552302481843 28.812432169279006 28.196479863782088 21.566535664457064 96-97 21.432168983373664 28.92443315416054 28.314520668694705 21.328877193771092 98-99 22.288805871567067 30.921839527336214 28.552245449104397 18.237109151992325 100 21.570086381126533 31.732489072978158 22.4380676713984 24.259356874496905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45706.0 1 35610.0 2 18787.5 3 11300.5 4 10832.5 5 13222.5 6 16533.0 7 19754.0 8 24223.0 9 28073.5 10 29226.5 11 30854.0 12 36365.0 13 53016.5 14 87526.5 15 133629.5 16 172978.0 17 193033.0 18 199447.0 19 190066.5 20 173231.5 21 160143.5 22 153886.5 23 164788.0 24 192203.0 25 233950.0 26 287216.0 27 344539.5 28 399852.0 29 459849.0 30 523660.0 31 586368.5 32 652563.5 33 717026.0 34 780578.5 35 838576.5 36 898517.5 37 955033.0 38 992672.5 39 1020694.0 40 1044598.5 41 1060364.5 42 1068453.0 43 1080528.0 44 1087046.5 45 1089829.0 46 1086749.0 47 1073729.5 48 1058985.0 49 1027272.0 50 988005.0 51 945161.5 52 884947.5 53 814166.5 54 746400.0 55 683200.0 56 621601.0 57 557786.0 58 495020.0 59 434449.5 60 367075.5 61 295284.5 62 227809.5 63 174727.5 64 134769.5 65 102477.5 66 76581.5 67 58581.5 68 45434.5 69 33745.5 70 24640.0 71 18074.5 72 13068.5 73 9284.5 74 6544.5 75 4713.0 76 3413.0 77 2391.0 78 1700.0 79 1232.0 80 916.5 81 691.0 82 525.0 83 364.5 84 252.5 85 186.0 86 148.0 87 117.0 88 86.0 89 74.5 90 60.5 91 51.5 92 56.0 93 48.5 94 42.5 95 53.5 96 49.0 97 29.5 98 15.5 99 16.5 100 34.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.14470950507625005 6 0.006380530736663147 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.4140174103624876E-4 24-25 3.7566712544771054E-4 26-27 0.003156869892569379 28-29 6.303497643009041E-4 30-31 3.9117346798671545E-6 32-33 0.0 34-35 1.9691017997275392E-6 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0025234597851876888 48-49 0.0 50-51 3.1778387861621895E-5 52-53 0.0 54-55 2.3722008084374093E-5 56-57 1.6530309736678691E-4 58-59 1.2295878265711164E-4 60-61 1.6856159097478656E-4 62-63 0.0 64-65 7.463170947549061E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.985181630032876E-4 76-77 2.8968538959667346E-5 78-79 3.3942943718643373E-4 80-81 0.0 82-83 0.0 84-85 7.609310214253572E-6 86-87 0.0 88-89 6.694760521512988E-4 90-91 0.0 92-93 1.696750456244078E-4 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 8512.0 20-21 21510.0 22-23 26316.0 24-25 28072.0 26-27 33640.0 28-29 49637.0 30-31 75063.0 32-33 88318.0 34-35 118441.0 36-37 168546.0 38-39 203584.0 40-41 211998.0 42-43 234911.0 44-45 278663.0 46-47 268863.0 48-49 246507.0 50-51 270937.0 52-53 184427.0 54-55 191981.0 56-57 213434.0 58-59 223632.0 60-61 227992.0 62-63 213408.0 64-65 218133.0 66-67 234028.0 68-69 243513.0 70-71 246057.0 72-73 228267.0 74-75 222427.0 76-77 231913.0 78-79 251143.0 80-81 261615.0 82-83 250697.0 84-85 246558.0 86-87 262016.0 88-89 293512.0 90-91 323602.0 92-93 347016.0 94-95 398732.0 96-97 890983.0 98-99 1592033.0 100-101 1.5419572E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.06461271302207 #Duplication Level Percentage of deduplicated Percentage of total 1 88.62326350137107 72.72833796604121 2 7.531331320367365 12.361115760388019 3 1.9562051166610412 4.816056458580615 4 0.8026684573981387 2.634827043733485 5 0.386699329821077 1.586716536907594 6 0.21540310539471536 1.0606183452839753 7 0.13254919951188684 0.7614319106354862 8 0.0817226859007208 0.5365232454648481 9 0.06542582615751599 0.4832230576541453 >10 0.19898007899341047 2.551050524994128 >50 0.004461812222967145 0.24212596639912673 >100 0.0012033719828745328 0.1563639171174099 >500 5.757007554596793E-5 0.03456873888157253 >1k 2.8624141651602332E-5 0.04704052791838479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.7669272509594E-6 2 7.7669272509594E-6 5.43684907567158E-5 0.0 3.8834636254797E-6 1.1650390876439099E-5 3 7.7669272509594E-6 5.43684907567158E-5 0.0 7.7669272509594E-6 1.1650390876439099E-5 4 7.7669272509594E-6 1.475716177682286E-4 0.0 1.55338545019188E-5 1.55338545019188E-5 5 1.94173181273985E-5 1.475716177682286E-4 0.0 1.94173181273985E-5 1.55338545019188E-5 6 1.94173181273985E-5 1.475716177682286E-4 0.0 2.3300781752878198E-5 1.55338545019188E-5 7 1.94173181273985E-5 1.514550813937083E-4 0.0 3.10677090038376E-5 1.55338545019188E-5 8 1.94173181273985E-5 1.669889358956271E-4 0.0 3.49511726293173E-5 1.94173181273985E-5 9 1.94173181273985E-5 2.873763082854978E-4 0.0 4.6601563505756397E-5 1.94173181273985E-5 10-11 2.71842453783579E-5 2.8931804009823766E-4 0.0 5.2426758943975945E-5 1.94173181273985E-5 12-13 2.71842453783579E-5 2.912597719109775E-4 0.0 5.82519543821955E-5 2.135904994013835E-5 14-15 3.10677090038376E-5 3.631038489823519E-4 0.0 7.184407707137445E-5 2.71842453783579E-5 16-17 3.689290444205715E-5 3.7281250804605117E-4 0.0 8.93196633860331E-5 4.27180998802767E-5 18-19 4.6601563505756397E-5 3.922298261734497E-4 0.0 1.1067871332617145E-4 5.631022256945565E-5 20-21 4.6601563505756397E-5 4.155306079263279E-4 0.0 1.3397949507904964E-4 5.82519543821955E-5 22-23 4.8543295318496246E-5 4.815494895594828E-4 0.0 2.368912811542617E-4 6.796061344589474E-5 24-25 5.04850271312361E-5 5.30092784877979E-4 0.0 4.2523926699002715E-4 7.961100432233385E-5 26-27 5.04850271312361E-5 5.43684907567158E-4 0.0 6.543636208933294E-4 8.93196633860331E-5 28-29 5.04850271312361E-5 5.495101030053776E-4 0.0 0.0022737679527183646 9.126139519877294E-5 30-31 5.43684907567158E-5 5.864030074474347E-4 0.0 0.00659412123606453 9.320312701151279E-5 32-33 5.43684907567158E-5 5.922282028856542E-4 0.0 0.014227068991944881 9.320312701151279E-5 34-35 5.43684907567158E-5 6.87373061709907E-4 0.0 0.023392043148076976 9.70865906369925E-5 36-37 5.43684907567158E-5 7.611588705940212E-4 0.0 0.03890259686824289 9.70865906369925E-5 38-39 5.43684907567158E-5 7.941683114105986E-4 0.0 0.07770034021859784 1.009700542624722E-4 40-41 5.43684907567158E-5 9.009635611112904E-4 0.0 0.12169221616803189 1.048535178879519E-4 42-43 5.43684907567158E-5 9.495068564297866E-4 0.0 0.14957742673078886 1.0679524970069176E-4 44-45 5.43684907567158E-5 0.0010757194242578768 0.0 0.18334220122252212 1.126204451389113E-4 46-47 5.631022256945565E-5 0.00116503908764391 0.0 0.21828172346096297 1.1844564057713084E-4 48-49 5.82519543821955E-5 0.001178631210333089 0.0 0.25185814996686046 1.281542996408301E-4 50-51 5.82519543821955E-5 0.0013630957325433746 0.0 0.28532195602761906 1.3397949507904964E-4 52-53 5.82519543821955E-5 0.001370862659794334 0.0 0.3615815312411639 1.514550813937083E-4 54-55 5.82519543821955E-5 0.0013922217097344725 0.0 0.4486196597472277 1.708723995211068E-4 56-57 5.82519543821955E-5 0.0014291146141765295 0.0 0.5330364503060927 1.7281413133384666E-4 58-59 5.82519543821955E-5 0.0014485319323039281 0.0 0.5792826768901177 1.8058105858480603E-4 60-61 5.82519543821955E-5 0.0014582405913676274 0.0 0.6076183692334303 1.8640625402302559E-4 62-63 5.82519543821955E-5 0.001464065786805847 0.0 0.6287793625286692 1.8640625402302559E-4 64-65 5.82519543821955E-5 0.0014990169594351643 0.0 0.6370220140737499 1.8834798583576546E-4 66-67 5.82519543821955E-5 0.001502900423060644 0.0 0.6435947762598743 1.9223144946124514E-4 68-69 5.82519543821955E-5 0.0015339681320644814 0.0 0.6499306471648443 2.019401085249444E-4 70-71 5.82519543821955E-5 0.0015475602547536603 0.0 0.6554626410993402 2.058235721504241E-4 72-73 5.82519543821955E-5 0.00155144371837914 0.0 0.659897556559638 2.213574266523429E-4 74-75 5.82519543821955E-5 0.0015922200864466772 0.0 0.6636062643219711 2.213574266523429E-4 76-77 5.82519543821955E-5 0.0017087239952110682 0.0 0.6669693438216365 2.252408902778226E-4 78-79 5.82519543821955E-5 0.001747558631465865 0.0 0.6689324346843166 2.252408902778226E-4 80-81 5.82519543821955E-5 0.0017514420950913447 0.0 0.6695867983052098 2.3494954934152185E-4 82-83 5.82519543821955E-5 0.001759209022342304 0.0 0.670347957175804 2.524251356561805E-4 84-85 5.82519543821955E-5 0.0017747428768442228 0.0 0.6706935854384716 2.563085992816602E-4 86-87 5.82519543821955E-5 0.0018271696357881988 0.0 0.6708372735926144 2.640755265326196E-4 88 5.82519543821955E-5 0.002019401085249444 0.0 0.6708528074471163 2.71842453783579E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 39300 0.0 54.1446 1 GTATCAA 73825 0.0 36.865875 1 TCAACGC 92335 0.0 29.03782 4 ATCAACG 92205 0.0 29.029055 3 CAACGCA 93885 0.0 28.500727 5 TATCAAC 94645 0.0 28.469973 2 AACGCAG 96880 0.0 27.703323 6 ACGCAGA 112775 0.0 23.686531 7 CGCAGAG 113750 0.0 23.47253 8 GCAGAGT 129775 0.0 20.423386 9 GAGTACT 74000 0.0 19.294415 12-13 GTACATG 94580 0.0 19.150148 1 TACATGG 93465 0.0 18.964016 2 GTGGTAT 15535 0.0 18.291737 1 CAGAGTA 128540 0.0 18.290598 10-11 ACATGGG 94365 0.0 18.288622 3 AGAGTAC 120380 0.0 17.876541 10-11 GTACTTT 81075 0.0 17.565262 14-15 TGGTATC 14740 0.0 17.553993 2 AGTACTT 80565 0.0 16.7125 12-13 >>END_MODULE