FastQCFastQC Report
Fri 27 May 2016
SRR2139850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30176738
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA476150.055.970061
GTATCAA898900.037.2726141
ATCAACG1105850.029.8654773
TCAACGC1110400.029.7280044
CAACGCA1129050.029.188715
TATCAAC1143900.029.0222152
AACGCAG1167400.028.363336
ACGCAGA1361600.024.1933547
CGCAGAG1377150.023.91418
GCAGAGT1580000.020.6055879
GAGTACT862550.019.71869312-13
GTGGTAT184800.019.5878121
GTACATG1227000.019.3637391
TACATGG1208850.019.2316462
TGGTATC173400.019.1359582
CAGAGTA1551750.018.77023310-11
ACATGGG1235300.018.4840953
GTACTTT936300.018.12588914-15
AGAGTAC1465550.017.7349810-11
AGTACTT933450.016.54719712-13
Produced by FastQC (version 0.11.5)