##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30176738
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.770283620449632	31.0	30.0	33.0	28.0	34.0
2	31.232746826379977	31.0	31.0	34.0	28.0	34.0
3	31.392778802003054	31.0	31.0	34.0	28.0	34.0
4	35.07952426799742	37.0	35.0	37.0	32.0	37.0
5	35.13782394240226	37.0	35.0	37.0	33.0	37.0
6	35.00101999096125	37.0	35.0	37.0	32.0	37.0
7	35.01786813405743	37.0	35.0	37.0	32.0	37.0
8	35.1180645833887	37.0	35.0	37.0	32.0	37.0
9	36.822750855311135	39.0	37.0	39.0	32.0	39.0
10-11	36.804057632074084	39.0	37.0	39.0	32.0	39.0
12-13	36.71842388995126	39.0	37.0	39.0	32.0	39.0
14-15	37.876232513931754	40.0	37.0	41.0	32.0	41.0
16-17	37.91850048537387	40.0	37.0	41.0	32.5	41.0
18-19	37.97680115392194	40.0	37.0	41.0	32.5	41.0
20-21	37.9898093137207	40.0	37.0	41.0	32.5	41.0
22-23	37.9384191248315	40.0	37.0	41.0	32.0	41.0
24-25	37.96441100064338	40.0	37.0	41.0	32.0	41.0
26-27	37.84784979047019	40.0	37.0	41.0	32.0	41.0
28-29	37.805337186284746	40.0	37.0	41.0	32.0	41.0
30-31	37.728653874836844	40.0	37.0	41.0	32.0	41.0
32-33	37.636148064746216	40.0	37.0	41.0	32.0	41.0
34-35	37.55447396028519	40.0	37.0	41.0	32.0	41.0
36-37	37.45879208587429	40.0	36.0	41.0	31.0	41.0
38-39	37.43481750642352	40.0	36.0	41.0	31.0	41.0
40-41	37.3624162379282	40.0	36.0	41.0	31.0	41.0
42-43	37.31543295399527	39.0	36.0	41.0	31.0	41.0
44-45	37.252278126480995	39.0	36.0	41.0	31.0	41.0
46-47	37.12532513290005	39.0	36.0	41.0	30.5	41.0
48-49	37.05719782381267	39.0	36.0	41.0	30.0	41.0
50-51	36.9890832075499	39.0	35.5	41.0	30.0	41.0
52-53	36.9085030039095	39.0	35.0	41.0	30.0	41.0
54-55	36.773673633112196	39.0	35.0	40.5	30.0	41.0
56-57	36.63168986963166	39.0	35.0	40.0	30.0	41.0
58-59	36.46330852168662	38.0	35.0	40.0	30.0	41.0
60-61	36.30646403166345	38.0	35.0	40.0	29.0	41.0
62-63	36.13523712447029	38.0	34.0	40.0	29.0	41.0
64-65	35.96605999085915	38.0	34.0	40.0	29.0	41.0
66-67	35.71939652625982	37.0	34.0	40.0	29.0	41.0
68-69	35.4283458655146	37.0	34.0	39.5	29.0	41.0
70-71	35.14652041278949	36.0	34.0	39.0	28.0	41.0
72-73	34.87893252139308	36.0	33.5	39.0	28.0	40.0
74-75	34.5744275558733	35.5	33.0	38.5	28.0	40.0
76-77	34.27038072565391	35.0	33.0	37.5	28.0	39.5
78-79	34.22628219707401	35.0	33.0	37.0	29.0	39.0
80-81	33.97040419434432	35.0	33.0	37.0	29.0	39.0
82-83	33.75868964778178	35.0	33.0	36.0	29.0	38.5
84-85	33.533795013911856	35.0	33.0	36.0	29.0	37.0
86-87	33.31845451105371	35.0	33.0	35.5	29.0	37.0
88-89	33.16308349187145	35.0	32.5	35.0	29.0	36.5
90-91	33.047670488066196	35.0	32.0	35.0	29.0	36.0
92-93	32.98692554082903	35.0	32.0	35.0	29.0	36.0
94-95	32.948271277877275	34.5	32.0	35.0	29.0	36.0
96-97	32.99619380266585	34.0	32.0	35.0	29.0	35.5
98-99	33.1124743663367	34.0	32.0	35.0	29.0	35.0
100	32.67044412282482	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	8.0
17	356.0
18	3257.0
19	11479.0
20	24277.0
21	41899.0
22	67035.0
23	103241.0
24	157017.0
25	226756.0
26	312355.0
27	410161.0
28	517545.0
29	639205.0
30	775913.0
31	946860.0
32	1165987.0
33	1469858.0
34	1919841.0
35	2558929.0
36	3621389.0
37	5435950.0
38	6447599.0
39	3205202.0
40	114615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.21663845840462	19.48578073614186	9.793228810880752	29.504351994572776
2	13.847139475446285	23.389479671394568	37.44341750920858	25.319963343950562
3	17.20873210351629	27.99902030497796	29.287598944591032	25.504648646914717
4	11.737070454732383	20.164800449935974	38.4402383054126	29.657890789919044
5	12.658222597150385	39.44654696251773	34.7299986260992	13.165231814232683
6	27.57862168797357	40.65345744539829	18.783793009284544	12.98412785734359
7	25.07942707392694	34.9844671746827	22.94281774259365	16.993288008796707
8	24.546145444878768	37.35153879123715	20.802278231663077	17.300037532221012
9	26.094271687019322	17.138051833170305	21.052974645569712	35.714701834240664
10-11	23.31389164726817	27.997713006621193	28.904338500735232	19.7840568453754
12-13	24.522108718311436	25.83193219890102	29.10618602978228	20.539773053005263
14-15	21.858964676698985	26.6688931056763	27.22800754674014	24.244134670884574
16-17	20.772279959484024	29.662747179632206	27.93928555167228	21.625687309211486
18-19	20.75903300084986	28.770409180740476	29.91096320616231	20.559594612247352
20-21	21.947084944534513	27.77871090258401	29.73116640260054	20.54303775028094
22-23	21.534107176125843	27.708613308115815	29.63487409435227	21.122405421406075
24-25	21.092683340211536	27.855213835536507	29.840222561579406	21.21188026267255
26-27	21.05985276725716	28.03261076743892	29.692863345168714	21.21467312013521
28-29	21.047145884492355	28.013690384682782	29.793209744131367	21.145953986693495
30-31	21.39085745120333	27.861552037555576	29.68687938639204	21.060711124849053
32-33	21.15619491986765	27.99643026150271	29.655359786053786	21.192015032575853
34-35	21.283200554019068	28.039861427451072	29.712931624210825	20.96400639431904
36-37	21.255352687113625	27.916605170592707	29.68916042033729	21.13888172195638
38-39	21.09973773905027	28.07262386369287	29.68631363748453	21.14132475977233
40-41	21.364695295921184	27.956797777572323	29.537271165781544	21.141235760724953
42-43	21.238154512731988	28.02915116350142	29.341867162443624	21.390827161322964
44-45	21.280687550600984	28.257240921873166	29.13906620252762	21.323005324998228
46-47	21.378005829903646	28.235884658906134	28.81634866260639	21.56976084858383
48-49	21.3538439919465	28.378988684655027	28.711077783377114	21.55608954002136
50-51	21.37049347762956	28.497235780836842	28.553919131417516	21.57835161011608
52-53	21.50068896550247	28.405936228976614	28.472679280324787	21.620695525196126
54-55	21.420089204929727	28.317423833317807	28.627813598473324	21.634673363279145
56-57	21.4217016534509	28.39563238911873	28.5603766157559	21.622289341674474
58-59	21.417772971091868	28.445493303713754	28.52202861536876	21.614705109825614
60-61	21.548999743169546	28.42570896733244	28.50451865840668	21.520772631091337
62-63	21.43793121822728	28.479923304709875	28.53273687005463	21.54940860700821
64-65	21.368179397183475	28.415438491047734	28.52592753290466	21.69045457886413
66-67	21.462911891402754	28.56224325779365	28.30481475587287	21.670030094930723
68-69	21.440240092240142	28.500074224941287	28.364357548506568	21.695328134312
70-71	21.438796920466963	28.265406409435094	28.441350044588283	21.854446625509663
72-73	21.45244966300053	28.323144559366	28.41117594976185	21.81322982787162
74-75	21.37116641194515	28.434374078024998	28.37520228107519	21.819257228954655
76-77	21.475929765536083	28.36223099463961	28.31769728350476	21.844141956319547
78-79	21.464838209378776	28.423725571352726	28.314304451234516	21.797131768033985
80-81	21.451522046567415	28.468537041381225	28.243006111063167	21.836934800988196
82-83	21.51314128517522	28.416867133150557	28.34049003606286	21.729501545611367
84-85	21.563924730236405	28.448694202186957	28.280522948042385	21.706858119534253
86-87	21.352403355215802	28.563740468117267	28.38038562942991	21.703470547237018
88-89	21.438023231424296	28.48195344508268	28.339810024224683	21.740213299268344
90-91	21.483387282659585	28.401192543923067	28.43806037536029	21.677359798057058
92-93	21.46939605660856	28.452922259411118	28.413488073746944	21.664193610233376
94-95	21.270610516934045	28.54607465638397	28.500238468790972	21.683076357891014
96-97	21.307372579597754	28.668004772827675	28.601442347108197	21.42318030046637
98-99	22.242443678262845	30.640734304042	28.84802246421751	18.268799553477642
100	21.457072629464115	31.55595057655367	22.628942921420247	24.35803387256197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45274.0
1	38367.0
2	22525.0
3	12957.5
4	12643.0
5	15472.0
6	20688.0
7	29318.5
8	41670.0
9	49492.0
10	47094.5
11	42593.0
12	46172.5
13	60673.0
14	95419.5
15	143205.5
16	182404.5
17	205931.0
18	212629.0
19	204076.5
20	185911.0
21	166532.0
22	156782.0
23	166509.0
24	196464.0
25	242399.0
26	304452.5
27	375768.5
28	443910.5
29	515704.5
30	590704.0
31	665013.5
32	746338.0
33	826382.5
34	905424.0
35	977414.0
36	1047156.0
37	1111961.5
38	1157589.5
39	1191338.5
40	1218067.0
41	1235861.5
42	1248370.5
43	1262316.0
44	1270493.0
45	1276429.0
46	1271714.5
47	1256876.5
48	1238782.0
49	1205207.5
50	1164633.5
51	1112462.0
52	1042241.5
53	964860.0
54	886284.5
55	809530.0
56	735775.0
57	660107.5
58	585246.0
59	514324.5
60	437225.5
61	352438.0
62	271290.5
63	207524.0
64	160088.0
65	122208.0
66	91579.0
67	69728.5
68	53270.5
69	39352.5
70	28938.0
71	21504.0
72	16112.0
73	11807.5
74	8383.5
75	5946.5
76	4176.0
77	2892.0
78	2093.5
79	1570.0
80	1134.0
81	796.5
82	588.5
83	412.5
84	290.0
85	214.5
86	165.5
87	145.5
88	108.0
89	79.0
90	64.5
91	51.0
92	45.0
93	39.5
94	34.5
95	46.0
96	49.0
97	30.0
98	18.5
99	24.5
100	41.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.14434297040322913
6	0.006157060448349322
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.315157595050085E-6
22-23	4.943329455473847E-4
24-25	4.4828773510989875E-4
26-27	0.003240900739055005
28-29	6.889477471408668E-4
30-31	5.00221681575218E-6
32-33	3.344079868398418E-6
34-35	1.6777881088512684E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.002579147091912117
48-49	0.0
50-51	2.517421274482641E-5
52-53	0.0
54-55	7.312148661539522E-6
56-57	1.751516324556196E-4
58-59	9.116875779888253E-5
60-61	1.9916580716913105E-4
62-63	0.0
64-65	8.098647732602641E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	3.025546321386479E-4
76-77	3.668343887838021E-5
78-79	3.4409038871273086E-4
80-81	0.0
82-83	0.0
84-85	8.545069473551087E-6
86-87	0.0
88-89	6.288328953486655E-4
90-91	0.0
92-93	1.0849701124639333E-4
94-95	0.0
96-97	2.3592820383894826E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	7800.0
20-21	20241.0
22-23	27584.0
24-25	29237.0
26-27	34192.0
28-29	51018.0
30-31	81193.0
32-33	95549.0
34-35	128414.0
36-37	184524.0
38-39	232589.0
40-41	239061.0
42-43	266289.0
44-45	317684.0
46-47	309023.0
48-49	281661.0
50-51	245161.0
52-53	218071.0
54-55	227645.0
56-57	244016.0
58-59	257468.0
60-61	259212.0
62-63	242783.0
64-65	247645.0
66-67	266165.0
68-69	275486.0
70-71	277217.0
72-73	258499.0
74-75	252561.0
76-77	262630.0
78-79	281699.0
80-81	296132.0
82-83	283749.0
84-85	279797.0
86-87	295312.0
88-89	326215.0
90-91	358933.0
92-93	385163.0
94-95	459862.0
96-97	1045450.0
98-99	1904534.0
100-101	1.8419274E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.89744309032639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.15772115575442	72.19891951326261
2	8.008326482158811	13.117229246427097
3	2.004818681736388	4.9256857168178705
4	0.7702104687952145	2.5231307214291876
5	0.392269959930913	1.6062953359743282
6	0.2084204560958901	1.0241461465183819
7	0.12968863942281914	0.7434817576614534
8	0.07781369433056089	0.509819408246813
9	0.05166368246324692	0.38080111449334125
>10	0.19346313473064816	2.432414676646523
>50	0.004000368321083409	0.22091186193403772
>100	0.0014993770008700585	0.1934147458094703
>500	4.8315210527476075E-5	0.028292128595695173
>1k	5.558404866108204E-5	0.0954576261831517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.627621580569775E-6	0.0
2	0.0	1.9882864741709327E-5	0.0	6.627621580569775E-6	0.0
3	0.0	1.9882864741709327E-5	0.0	1.9882864741709327E-5	0.0
4	0.0	8.28452697571222E-5	0.0	3.3138107902848874E-5	0.0
5	0.0	8.947289133769197E-5	0.0	3.9765729483418654E-5	0.0
6	0.0	8.947289133769197E-5	0.0	4.639335106398843E-5	0.0
7	3.3138107902848876E-6	9.278670212797686E-5	0.0	4.639335106398843E-5	0.0
8	3.3138107902848876E-6	1.0272813449883152E-4	0.0	4.639335106398843E-5	0.0
9	3.3138107902848876E-6	1.7563197188509905E-4	0.0	6.959002659598263E-5	0.0
10-11	3.3138107902848876E-6	1.7894578267538394E-4	0.0	9.278670212797686E-5	0.0
12-13	3.3138107902848876E-6	1.8391649886081128E-4	0.0	1.1432647226482862E-4	0.0
14-15	3.3138107902848876E-6	2.3196675531994213E-4	0.0	1.2758171542596818E-4	0.0
16-17	4.970716185427332E-6	2.4356509308593924E-4	0.0	1.6237672872395948E-4	0.0
18-19	8.28452697571222E-6	2.684186740130759E-4	0.0	2.1705460676366015E-4	0.0
20-21	9.941432370854664E-6	2.9824297112563986E-4	0.0	2.8001701177907297E-4	0.0
22-23	1.1598337765997107E-5	3.2641036284306144E-4	0.0	3.578915653507679E-4	0.0
24-25	1.325524316113955E-5	3.6948990311676494E-4	0.0	4.70561132220454E-4	0.0
26-27	1.325524316113955E-5	3.860589570681894E-4	0.0	6.478500095006955E-4	0.0
28-29	1.325524316113955E-5	3.9102967325361674E-4	0.0	0.0017331230433189964	0.0
30-31	1.325524316113955E-5	4.2913849734189295E-4	0.0	0.004969059280032189	0.0
32-33	1.325524316113955E-5	4.307954027370354E-4	0.0	0.01065887240695134	0.0
34-35	1.325524316113955E-5	4.838163753815936E-4	0.0	0.0176410717420816	0.0
36-37	1.325524316113955E-5	5.33523537235867E-4	0.0	0.02940344314219781	3.3138107902848876E-6
38-39	1.325524316113955E-5	5.368373480261518E-4	0.0	0.05714832398385803	3.3138107902848876E-6
40-41	1.325524316113955E-5	5.799168882998553E-4	0.0	0.08716979283844398	3.3138107902848876E-6
42-43	1.325524316113955E-5	5.8985832067071E-4	0.0	0.10761434850910659	3.3138107902848876E-6
44-45	1.325524316113955E-5	6.49506914895838E-4	0.0	0.13205204618206248	3.3138107902848876E-6
46-47	1.325524316113955E-5	7.041847929355387E-4	0.0	0.15722209603967136	3.3138107902848876E-6
48-49	1.325524316113955E-5	7.157831307015357E-4	0.0	0.18112792708078654	3.3138107902848876E-6
50-51	1.325524316113955E-5	8.499924677080737E-4	0.0	0.20464438535404322	3.3138107902848876E-6
52-53	1.325524316113955E-5	8.615908054740708E-4	0.0	0.25178831456203116	4.970716185427332E-6
54-55	1.6569053951424437E-5	8.682184270546406E-4	0.0	0.30402391404929185	6.627621580569775E-6
56-57	1.6569053951424437E-5	8.781598594254953E-4	0.0	0.35676984039825643	8.28452697571222E-6
58-59	1.6569053951424437E-5	8.930720079817772E-4	0.0	0.3931240016730768	9.941432370854664E-6
60-61	1.6569053951424437E-5	9.079841565380592E-4	0.0	0.4104171232821785	9.941432370854664E-6
62-63	1.6569053951424437E-5	9.179255889089139E-4	0.0	0.42943508340762343	9.941432370854664E-6
64-65	1.6569053951424437E-5	9.378084536506232E-4	0.0	0.43778920040993163	9.941432370854664E-6
66-67	1.6569053951424437E-5	9.411222644409082E-4	0.0	0.44346575829368967	1.1598337765997107E-5
68-69	1.9882864741709327E-5	9.709465615534721E-4	0.0	0.4491091780695448	1.325524316113955E-5
70-71	1.9882864741709327E-5	9.77574183134042E-4	0.0	0.45384295678346676	1.6569053951424437E-5
72-73	1.9882864741709327E-5	9.808879939243267E-4	0.0	0.4578062744886475	2.3196675531994214E-5
74-75	1.9882864741709327E-5	0.0010024277640611787	0.0	0.460975934509555	2.3196675531994214E-5
76-77	1.9882864741709327E-5	0.0010620763582863063	0.0	0.46409257355781797	2.3196675531994214E-5
78-79	1.9882864741709327E-5	0.001090243750003728	0.0	0.46603115287013464	2.3196675531994214E-5
80-81	2.3196675531994214E-5	0.0010919006553988704	0.0	0.4667833879195293	2.65104863222791E-5
82-83	2.3196675531994214E-5	0.0010985282769794403	0.0	0.46749254342865026	2.9824297112563987E-5
84-85	2.3196675531994214E-5	0.0011101266147454374	0.0	0.4677825018728002	2.9824297112563987E-5
86-87	2.3196675531994214E-5	0.0011416078172531438	0.0	0.46788688691269414	2.9824297112563987E-5
88	2.3196675531994214E-5	0.001265875721888827	0.0	0.467896828345065	2.9824297112563987E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	47615	0.0	55.97006	1
GTATCAA	89890	0.0	37.272614	1
ATCAACG	110585	0.0	29.865477	3
TCAACGC	111040	0.0	29.728004	4
CAACGCA	112905	0.0	29.18871	5
TATCAAC	114390	0.0	29.022215	2
AACGCAG	116740	0.0	28.36333	6
ACGCAGA	136160	0.0	24.193354	7
CGCAGAG	137715	0.0	23.9141	8
GCAGAGT	158000	0.0	20.605587	9
GAGTACT	86255	0.0	19.718693	12-13
GTGGTAT	18480	0.0	19.587812	1
GTACATG	122700	0.0	19.363739	1
TACATGG	120885	0.0	19.231646	2
TGGTATC	17340	0.0	19.135958	2
CAGAGTA	155175	0.0	18.770233	10-11
ACATGGG	123530	0.0	18.484095	3
GTACTTT	93630	0.0	18.125889	14-15
AGAGTAC	146555	0.0	17.73498	10-11
AGTACTT	93345	0.0	16.547197	12-13
>>END_MODULE