FastQCFastQC Report
Fri 27 May 2016
SRR2139849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32091135
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA532550.053.7554781
GTATCAA1001500.037.0903851
ATCAACG1239150.029.4616833
TCAACGC1246450.029.3092274
CAACGCA1261550.028.8823225
TATCAAC1284150.028.634032
AACGCAG1304800.028.0236386
ACGCAGA1501800.024.2377767
CGCAGAG1508800.024.1253268
GCAGAGT1713850.021.0760129
GAGTACT1014250.019.60824212-13
CAGAGTA1703050.018.70433810-11
GTACATG1200550.018.4358711
AGAGTAC1609350.018.24120710-11
TACATGG1185300.018.2153052
ACATGGG1184250.017.8155843
GTACTTT1115600.017.74568214-15
ACCGAAC31800.017.2957368
AGTACTT1100000.017.14407712-13
TGGTATC199950.016.991252
Produced by FastQC (version 0.11.5)