##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32091135
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.939723135376795	31.0	30.0	33.0	28.0	34.0
2	31.368198881092862	31.0	31.0	34.0	28.0	34.0
3	31.501534489197716	31.0	31.0	34.0	28.0	34.0
4	35.15626427672315	37.0	35.0	37.0	32.0	37.0
5	35.19145165791113	37.0	35.0	37.0	33.0	37.0
6	35.05776408344548	37.0	35.0	37.0	32.0	37.0
7	35.07883357818289	37.0	35.0	37.0	32.0	37.0
8	35.18018923294549	37.0	35.0	37.0	32.0	37.0
9	36.89961623357977	39.0	37.0	39.0	33.0	39.0
10-11	36.88504410953368	39.0	37.0	39.0	33.0	39.0
12-13	36.8055048068571	39.0	37.0	39.0	32.0	39.0
14-15	37.9808457226583	40.0	37.0	41.0	33.0	41.0
16-17	38.02580479001443	40.0	37.0	41.0	33.0	41.0
18-19	38.08941614249542	40.0	37.5	41.0	33.0	41.0
20-21	38.10865286752396	40.0	38.0	41.0	33.0	41.0
22-23	38.05920254128431	40.0	38.0	41.0	33.0	41.0
24-25	38.08576122264317	40.0	37.5	41.0	33.0	41.0
26-27	37.97205534513821	40.0	37.0	41.0	33.0	41.0
28-29	37.93224304318484	40.0	37.0	41.0	33.0	41.0
30-31	37.85288455030624	40.0	37.0	41.0	33.0	41.0
32-33	37.76164006212598	40.0	37.0	41.0	32.5	41.0
34-35	37.682247830621534	40.0	37.0	41.0	32.0	41.0
36-37	37.58963254106604	40.0	37.0	41.0	32.0	41.0
38-39	37.56960190825927	40.0	37.0	41.0	32.0	41.0
40-41	37.50327970987342	40.0	36.5	41.0	31.0	41.0
42-43	37.4640758804142	40.0	36.0	41.0	31.0	41.0
44-45	37.40623828282368	40.0	36.0	41.0	31.0	41.0
46-47	37.28920115045858	39.5	36.0	41.0	31.0	41.0
48-49	37.23301661499147	39.0	36.0	41.0	31.0	41.0
50-51	37.18310429481289	39.0	36.0	41.0	31.0	41.0
52-53	37.11964022498127	39.0	36.0	41.0	31.0	41.0
54-55	36.99081437327797	39.0	35.5	41.0	31.0	41.0
56-57	36.85996082622721	39.0	35.0	40.5	30.0	41.0
58-59	36.70051980706279	39.0	35.0	40.0	30.0	41.0
60-61	36.550728903527755	38.5	35.0	40.0	30.0	41.0
62-63	36.38679282716551	38.0	35.0	40.0	30.0	41.0
64-65	36.22161950683133	38.0	34.5	40.0	29.5	41.0
66-67	35.98428293740235	37.5	34.0	40.0	29.0	41.0
68-69	35.69783649095402	37.0	34.0	40.0	29.0	41.0
70-71	35.41975208511782	36.5	34.0	39.0	29.0	41.0
72-73	35.152578871882156	36.0	34.0	39.0	29.0	40.5
74-75	34.84814576213944	36.0	34.0	39.0	29.0	40.0
76-77	34.53923408211057	35.0	33.0	38.0	29.0	40.0
78-79	34.487360320934044	35.0	33.0	37.0	29.0	39.0
80-81	34.22261494783746	35.0	33.0	37.0	29.0	39.0
82-83	33.99712768937345	35.0	33.0	36.5	29.0	39.0
84-85	33.75757141110509	35.0	33.0	36.0	29.0	37.5
86-87	33.52919031549347	35.0	33.0	36.0	29.0	37.0
88-89	33.35608391398579	35.0	33.0	35.0	29.0	37.0
90-91	33.2233279200451	35.0	33.0	35.0	29.0	36.0
92-93	33.14407858998004	35.0	33.0	35.0	29.0	36.0
94-95	33.09372668467863	35.0	33.0	35.0	29.0	36.0
96-97	33.12537970281039	35.0	33.0	35.0	29.0	36.0
98-99	33.22518734221517	35.0	33.0	35.0	29.0	35.0
100	32.786022100455575	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	4.0
16	7.0
17	364.0
18	3701.0
19	12653.0
20	26638.0
21	44667.0
22	68879.0
23	103817.0
24	152202.0
25	217114.0
26	297599.0
27	391789.0
28	499667.0
29	620825.0
30	765592.0
31	940724.0
32	1166981.0
33	1480094.0
34	1955025.0
35	2633144.0
36	3814776.0
37	5800596.0
38	7123069.0
39	3819494.0
40	151712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.618774156788156	19.844854349963004	9.821615844998938	28.714755648249895
2	13.79729012389247	23.795256852087032	37.73928531976198	24.66816770425851
3	17.042921666684585	28.575165696071515	29.71883044959301	24.66308218765089
4	11.429673646631693	20.261629886259865	39.569429376679885	28.739267090428555
5	12.252676088714223	39.71584313236703	35.238982349958704	12.79249842896005
6	26.772046740427236	41.6164584731266	18.961395307034934	12.650099479411233
7	24.190711858586493	35.402213103400676	23.69093209074718	16.716142947265656
8	23.964696169206853	37.64249223344702	21.321287015869025	17.071524581477096
9	25.613737251736346	17.15673814590852	21.683707977296535	35.5458166250586
10-11	22.724077848913726	28.283223700252424	29.546119200832255	19.446579250001598
12-13	24.022258795147007	26.17542040815945	29.760704942346226	20.041615854347313
14-15	21.448906372429644	27.109321624180634	27.81912512598884	23.622646877400875
16-17	20.26360862587129	30.153885177323893	28.470968695871928	21.11153750093289
18-19	20.215947803278816	29.133976238826957	30.6118236486778	20.03825230921643
20-21	21.30591567720789	28.151211551480827	30.468580159840027	20.074292611471265
22-23	20.929725969020524	28.091178541450606	30.37594789398252	20.603147595546346
24-25	20.58314454853285	28.2281794916251	30.510845986578378	20.677829973263677
26-27	20.569653506430928	28.41352206027953	30.325425925805227	20.691398507484312
28-29	20.55787289047438	28.429318013959854	30.40735778917124	20.605451306394528
30-31	20.864807845310782	28.283097228423674	30.31071057940391	20.54138434686163
32-33	20.64819258982675	28.447360405699396	30.280090147382865	20.62435685709099
34-35	20.766544594468012	28.490456406714987	30.30443554767046	20.43856345114654
36-37	20.75371777529453	28.36197871431455	30.257237454066665	20.62706605632425
38-39	20.596593697172143	28.51584842251549	30.290754965887285	20.59680291442508
40-41	20.854678515420805	28.36815684364753	30.123260797236796	20.653903843694874
42-43	20.775124834419564	28.491469600622437	29.8946652279248	20.838740337033197
44-45	20.832923613654224	28.72051078672889	29.67653522148351	20.770030378133374
46-47	20.922615428862517	28.76755363333955	29.291044020374297	21.01878691742364
48-49	20.939894255291264	28.86115293262031	29.174242432380755	21.024710379707674
50-51	20.967289013506786	28.996634304123543	28.924263466682724	21.111813215686944
52-53	21.071815197480838	29.024711681531162	28.71879715362046	21.184675967367536
54-55	21.057000056694818	28.970111918900628	28.806045220287096	21.166842804117458
56-57	21.09878817624324	29.06140822823688	28.708715042208656	21.13108855331123
58-59	21.07030358027888	29.070424438402636	28.75597081336352	21.103301167954967
60-61	21.19284926392745	29.063090195068884	28.686729938081278	21.057330602922384
62-63	21.081637611953095	29.148732286495456	28.7097153032539	21.05991479829755
64-65	21.031445950037476	29.03601452438866	28.725830132304043	21.206709393269826
66-67	21.14118679403437	29.139726569461523	28.555401812286256	21.163684824217853
68-69	21.07713436725015	29.050308866527054	28.684877141770315	21.187679624452485
70-71	21.099239411783785	28.83720795079114	28.7286791681016	21.33487346932348
72-73	21.109815337070927	28.931920900255818	28.698109285630135	21.260154477043116
74-75	20.997395304718413	29.064999999617147	28.698959033075123	21.238645662589317
76-77	21.06741995104554	29.050473953239447	28.640399370930837	21.241706724784173
78-79	21.035747978853603	29.048218784614505	28.664507849940808	21.251525386591087
80-81	21.03787643915687	29.08745754873972	28.616396730538813	21.258269281564594
82-83	21.092297214528994	29.018903018225085	28.726134015275445	21.162665751970472
84-85	21.174412942063505	29.057339438015017	28.616031776421192	21.152215843500283
86-87	20.96788389399439	29.137218522469148	28.759891147748817	21.135006435787638
88-89	21.05995406761408	29.100253297549138	28.625417524174672	21.21437511066211
90-91	21.1560800241092	29.06143153849851	28.677612417074265	21.104876020318024
92-93	21.099270097936635	29.113001375010022	28.6550313024503	21.132697224603042
94-95	20.90940544443629	29.175702840823558	28.680355433636013	21.234536281104138
96-97	20.988611199270725	29.369842270988535	28.7200899923023	20.921456537438438
98-99	21.82071094729578	31.311642774708616	29.028387768536472	17.839258509459132
100	21.105656258597595	32.21978556805523	22.858407181243745	23.816150992103434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24140.0
1	19774.0
2	11686.0
3	7686.0
4	8101.5
5	12314.0
6	18284.5
7	24310.0
8	31854.5
9	37928.0
10	39706.5
11	42941.5
12	52121.5
13	77907.5
14	127569.5
15	191200.0
16	244347.5
17	268726.5
18	274080.5
19	255815.5
20	230121.0
21	216047.0
22	212316.0
23	231180.0
24	271082.5
25	329650.5
26	406677.0
27	488838.0
28	563944.0
29	643993.5
30	728468.5
31	812344.5
32	898302.5
33	977317.5
34	1054750.0
35	1126345.5
36	1197355.0
37	1263079.5
38	1303074.5
39	1325978.0
40	1341462.0
41	1343292.5
42	1337037.0
43	1339434.5
44	1339056.5
45	1335250.5
46	1324242.5
47	1304525.0
48	1275886.0
49	1222328.5
50	1163869.5
51	1099057.5
52	1016755.0
53	929587.5
54	844569.0
55	761451.5
56	683381.5
57	606797.5
58	534280.0
59	466899.5
60	391678.5
61	312299.5
62	238802.0
63	180907.0
64	137967.0
65	103782.0
66	76687.0
67	57973.0
68	43793.0
69	31648.5
70	22886.0
71	16787.5
72	12395.5
73	9049.0
74	6411.0
75	4592.0
76	3334.5
77	2317.5
78	1627.5
79	1164.5
80	806.5
81	563.0
82	437.5
83	335.5
84	249.0
85	187.0
86	144.5
87	107.5
88	79.5
89	65.5
90	61.0
91	60.5
92	50.0
93	40.5
94	36.0
95	48.0
96	49.0
97	26.5
98	13.0
99	13.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.14668848577652363
6	0.006490889150539549
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.5580626861592775E-6
20-21	3.117673856147286E-6
22-23	4.914213204122703E-4
24-25	3.8722802071894754E-4
26-27	0.0032946901707279373
28-29	6.620242334674941E-4
30-31	9.410005809737587E-6
32-33	3.1453855880225224E-6
34-35	3.1565633150493083E-6
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0024420377525091524
48-49	0.0
50-51	3.907711077774749E-5
52-53	0.0
54-55	5.198180006285293E-6
56-57	2.1160462127704405E-4
58-59	7.240180430240835E-5
60-61	1.605394009711278E-4
62-63	0.0
64-65	8.289111408711217E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.928809204944879E-4
76-77	4.447766474778418E-5
78-79	2.854242086999918E-4
80-81	0.0
82-83	0.0
84-85	1.4184742368299581E-5
86-87	0.0
88-89	7.064041729704582E-4
90-91	0.0
92-93	8.789834457831863E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	9881.0
20-21	24520.0
22-23	27545.0
24-25	30045.0
26-27	38056.0
28-29	59264.0
30-31	85374.0
32-33	104231.0
34-35	141634.0
36-37	199843.0
38-39	258703.0
40-41	259747.0
42-43	293597.0
44-45	352164.0
46-47	339800.0
48-49	311467.0
50-51	386650.0
52-53	249123.0
54-55	259871.0
56-57	276030.0
58-59	280783.0
60-61	279824.0
62-63	253718.0
64-65	258843.0
66-67	277581.0
68-69	288097.0
70-71	291518.0
72-73	268715.0
74-75	261473.0
76-77	274231.0
78-79	295890.0
80-81	310954.0
82-83	295805.0
84-85	289210.0
86-87	307686.0
88-89	345667.0
90-91	382339.0
92-93	408272.0
94-95	482187.0
96-97	1056713.0
98-99	1962812.0
100-101	1.9511272E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.90564763580387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.91822437137206	70.251626573844
2	7.654157281015034	12.232207892916199
3	2.1247555612005793	5.093399075565245
4	0.9125480757167033	2.9167097995579905
5	0.47841806416993327	1.9114152629083045
6	0.266379706715081	1.2771145789262424
7	0.1723501055357253	0.9640222762051894
8	0.10307073975834355	0.6588747370153472
9	0.07929504662242119	0.5702509849208244
>10	0.2835242819189346	3.5238833753195746
>50	0.0055419275219325565	0.2973452534168553
>100	0.0016284673772114912	0.19639087886935552
>500	3.56629431959779E-5	0.020674167655403027
>1k	7.070813284483595E-5	0.08608514287943195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.1161253723185545E-6	3.1161253723185545E-6
2	3.1161253723185545E-6	3.1161253723185543E-5	0.0	3.1161253723185545E-6	3.1161253723185545E-6
3	3.1161253723185545E-6	3.1161253723185543E-5	0.0	1.5580626861592772E-5	3.1161253723185545E-6
4	3.1161253723185545E-6	6.855475819100821E-5	0.0	1.869675223391133E-5	3.1161253723185545E-6
5	3.1161253723185545E-6	6.855475819100821E-5	0.0	1.869675223391133E-5	3.1161253723185545E-6
6	3.1161253723185545E-6	6.855475819100821E-5	0.0	2.1812877606229882E-5	3.1161253723185545E-6
7	6.232250744637109E-6	6.855475819100821E-5	0.0	2.1812877606229882E-5	3.1161253723185545E-6
8	6.232250744637109E-6	6.855475819100821E-5	0.0	3.1161253723185543E-5	3.1161253723185545E-6
9	9.348376116955664E-6	1.2776114026506074E-4	0.0	6.543863281868965E-5	6.232250744637109E-6
10-11	9.348376116955664E-6	1.2776114026506074E-4	0.0	7.634507162180459E-5	1.5580626861592772E-5
12-13	9.348376116955664E-6	1.2776114026506074E-4	0.0	9.504182385571591E-5	1.869675223391133E-5
14-15	9.348376116955664E-6	1.8852558502527257E-4	0.0	1.0594826265883085E-4	2.4929002978548436E-5
16-17	9.348376116955664E-6	1.9475783576990965E-4	0.0	1.3555145369585712E-4	2.4929002978548436E-5
18-19	1.0906438803114941E-5	2.1657071337613954E-4	0.0	1.6359658204672414E-4	3.116125372318555E-5
20-21	1.4022564175433495E-5	2.3682552829621013E-4	0.0	2.1033846263150246E-4	4.050962984014121E-5
22-23	1.5580626861592772E-5	2.8200934619482915E-4	0.0	2.6331259396091786E-4	4.050962984014121E-5
24-25	1.7138689547752052E-5	3.0538028648721834E-4	0.0	3.14728662604174E-4	4.3625755212459765E-5
26-27	1.869675223391133E-5	3.1784478797649256E-4	0.0	4.0198017302909357E-4	4.3625755212459765E-5
28-29	1.869675223391133E-5	3.240770387211297E-4	0.0	7.805894057657979E-4	4.674188058477832E-5
30-31	1.869675223391133E-5	3.583544178166338E-4	0.0	0.0017777495249077355	4.985800595709687E-5
32-33	1.869675223391133E-5	3.583544178166338E-4	0.0	0.0034542249752151175	4.985800595709687E-5
34-35	1.869675223391133E-5	4.1911886257684557E-4	0.0	0.005585654729881009	6.232250744637109E-5
36-37	1.869675223391133E-5	4.7988330733705746E-4	0.0	0.008683083349965651	6.232250744637109E-5
38-39	1.869675223391133E-5	5.00138122257128E-4	0.0	0.01386364178144525	6.855475819100821E-5
40-41	1.869675223391133E-5	5.593445043311806E-4	0.0	0.019394764317310687	6.855475819100821E-5
42-43	1.869675223391133E-5	5.811573819374104E-4	0.0	0.024006629868342143	6.855475819100821E-5
44-45	1.869675223391133E-5	6.590605162453743E-4	0.0	0.029048520720753566	7.167088356332676E-5
46-47	1.869675223391133E-5	7.151507729471083E-4	0.0	0.03441293054919996	7.167088356332676E-5
48-49	1.869675223391133E-5	7.213830236917454E-4	0.0	0.03941119564639892	7.167088356332676E-5
50-51	1.869675223391133E-5	8.024022833720279E-4	0.0	0.044372067239130064	8.101925968028242E-5
52-53	1.869675223391133E-5	8.101925968028242E-4	0.0	0.049974860658558815	8.569344773876026E-5
54-55	1.869675223391133E-5	8.25773223664417E-4	0.0	0.05511490946019828	8.725151042491953E-5
56-57	1.869675223391133E-5	8.444699758983283E-4	0.0	0.060008784357424565	1.0127407460035303E-4
58-59	1.869675223391133E-5	8.475861012706468E-4	0.0	0.06423580842497469	1.1373857608962726E-4
60-61	1.869675223391133E-5	8.507022266429654E-4	0.0	0.06826651659406874	1.1529663877578653E-4
62-63	2.1812877606229882E-5	8.600506027599211E-4	0.0	0.07207753792441432	1.1841276414810508E-4
64-65	2.1812877606229882E-5	8.865376684246288E-4	0.0	0.07518431492061592	1.1841276414810508E-4
66-67	2.1812877606229882E-5	8.896537937969474E-4	0.0	0.07791871493482547	1.1997082683426436E-4
68-69	2.1812877606229882E-5	9.036763579723809E-4	0.0	0.0804427764864035	1.2931920295122004E-4
70-71	2.1812877606229882E-5	9.130247340893365E-4	0.0	0.08241528384708113	1.3866757906817569E-4
72-73	2.1812877606229882E-5	9.176989221478143E-4	0.0	0.08427561069435531	1.4645789249897206E-4
74-75	2.1812877606229882E-5	9.457440504986813E-4	0.0	0.0857947218133606	1.4957401787129063E-4
76-77	2.1812877606229882E-5	0.0010127407460035303	0.0	0.08714088797420222	1.4957401787129063E-4
78-79	2.1812877606229882E-5	0.0010376697489820788	0.0	0.08801340307845142	1.4957401787129063E-4
80-81	2.1812877606229882E-5	0.0010376697489820788	0.0	0.08837331555895421	1.5269014324360917E-4
82-83	2.1812877606229882E-5	0.0010439019997267158	0.0	0.08882359567525425	1.5580626861592774E-4
84-85	2.1812877606229882E-5	0.0010563665012159901	0.0	0.08908379214384285	1.57364331302087E-4
86-87	2.1812877606229882E-5	0.0010968761310561313	0.0	0.08920376297067711	1.6359658204672412E-4
88	2.1812877606229882E-5	0.0012184050205765549	0.0	0.08921466940948022	1.6827077010520195E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	53255	0.0	53.755478	1
GTATCAA	100150	0.0	37.090385	1
ATCAACG	123915	0.0	29.461683	3
TCAACGC	124645	0.0	29.309227	4
CAACGCA	126155	0.0	28.882322	5
TATCAAC	128415	0.0	28.63403	2
AACGCAG	130480	0.0	28.023638	6
ACGCAGA	150180	0.0	24.237776	7
CGCAGAG	150880	0.0	24.125326	8
GCAGAGT	171385	0.0	21.076012	9
GAGTACT	101425	0.0	19.608242	12-13
CAGAGTA	170305	0.0	18.704338	10-11
GTACATG	120055	0.0	18.435871	1
AGAGTAC	160935	0.0	18.241207	10-11
TACATGG	118530	0.0	18.215305	2
ACATGGG	118425	0.0	17.815584	3
GTACTTT	111560	0.0	17.745682	14-15
ACCGAAC	3180	0.0	17.295736	8
AGTACTT	110000	0.0	17.144077	12-13
TGGTATC	19995	0.0	16.99125	2
>>END_MODULE