FastQCFastQC Report
Fri 27 May 2016
SRR2139848_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139848_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7592883
Sequences flagged as poor quality0
Sequence length19-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.049.5569231
GTATCAA171350.035.540391
ATCAACG216200.027.4801943
TCAACGC216850.027.321774
CAACGCA220700.026.8451565
TATCAAC223500.026.8417782
AACGCAG227500.026.1333686
ACGCAGA262250.022.5290157
CGCAGAG263150.022.3892928
GTACATG240800.020.3758751
TACATGG240500.020.1955642
ACATGGG240050.019.6831423
GCAGAGT304700.019.065589
AGAGTAC283500.018.3389110-11
CATGGGG157600.018.1819344
GAGTACT176150.017.6249612-13
TGGTATC41250.017.4920182
GTGGTAT44600.017.1950661
CAGAGTA305850.016.76288810-11
ATGGGGG94000.016.228765
Produced by FastQC (version 0.11.5)