##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7592883
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824969119107987	31.0	31.0	34.0	30.0	34.0
2	31.99008426706957	31.0	31.0	34.0	30.0	34.0
3	32.015510050661916	31.0	31.0	34.0	30.0	34.0
4	35.73012214201114	37.0	35.0	37.0	35.0	37.0
5	35.545678235790014	37.0	35.0	37.0	33.0	37.0
6	35.55116692829324	37.0	35.0	37.0	33.0	37.0
7	35.44537470154617	37.0	35.0	37.0	33.0	37.0
8	35.42773344459542	37.0	35.0	37.0	33.0	37.0
9	36.96272311847819	39.0	37.0	39.0	33.0	39.0
10-11	36.85336854262077	39.0	37.0	39.0	33.0	39.0
12-13	36.798035344413975	39.0	37.0	39.0	32.0	39.0
14-15	37.90904159856012	40.0	37.0	41.0	33.0	41.0
16-17	37.8942603224625	40.0	37.0	41.0	33.0	41.0
18-19	37.9399699692462	40.0	37.0	41.0	33.0	41.0
20-21	37.92413263229362	40.0	37.0	41.0	32.5	41.0
22-23	37.85823281506589	40.0	37.0	41.0	32.0	41.0
24-25	37.77114230802178	40.0	37.0	41.0	32.0	41.0
26-27	37.58370450353377	39.0	36.5	41.0	32.0	41.0
28-29	37.47932042200949	39.0	36.0	41.0	32.0	41.0
30-31	37.33004525179547	39.0	36.0	40.5	31.5	41.0
32-33	37.491782119209965	39.0	36.0	41.0	32.0	41.0
34-35	37.4481824290046	39.0	36.0	41.0	31.5	41.0
36-37	37.361557697669625	39.0	36.0	41.0	31.0	41.0
38-39	37.26113449185243	39.0	36.0	41.0	31.0	41.0
40-41	37.174460175470884	39.0	36.0	41.0	31.0	41.0
42-43	37.140504288995245	39.0	36.0	40.0	31.0	41.0
44-45	37.17502153495401	39.0	36.0	40.5	31.0	41.0
46-47	37.28685351410196	39.0	36.0	41.0	31.0	41.0
48-49	37.240233099164726	39.0	36.0	41.0	31.0	41.0
50-51	37.14826992685907	39.0	36.0	41.0	31.0	41.0
52-53	37.06767306640188	39.0	35.5	41.0	31.0	41.0
54-55	36.96066452360655	39.0	35.0	40.0	31.0	41.0
56-57	36.85647601516722	39.0	35.0	40.0	31.0	41.0
58-59	36.73378053915645	39.0	35.0	40.0	30.0	41.0
60-61	36.5401428671408	38.0	35.0	40.0	30.0	41.0
62-63	36.34907992709315	38.0	35.0	40.0	30.0	41.0
64-65	36.166378170075795	38.0	34.0	40.0	29.0	41.0
66-67	35.933808638885694	37.5	34.0	40.0	29.0	41.0
68-69	35.67172579224426	37.0	34.0	40.0	29.0	41.0
70-71	35.39136001468949	36.5	34.0	39.0	29.0	41.0
72-73	35.09089334534124	36.0	34.0	39.0	29.0	40.0
74-75	34.74636412583311	36.0	33.0	39.0	28.5	40.0
76-77	33.677930848437285	35.0	31.5	37.0	27.5	39.0
78-79	34.087043102620214	35.0	33.0	37.0	28.5	39.0
80-81	33.9658221171413	35.0	33.0	37.0	29.0	39.0
82-83	33.71312946234538	35.0	33.0	36.0	29.0	38.5
84-85	33.45980310637526	35.0	32.5	36.0	28.0	37.0
86-87	33.2240188650123	35.0	32.0	36.0	28.0	37.0
88-89	32.99483778501235	34.0	32.0	35.0	28.0	36.5
90-91	32.8493740636777	34.0	32.0	35.0	27.5	36.0
92-93	32.75611998096332	34.0	32.0	35.0	27.5	36.0
94-95	32.70512425646663	34.0	32.0	35.0	28.0	36.0
96-97	32.7051582833296	34.0	32.0	35.0	28.5	35.0
98-99	32.80963271898057	34.0	32.0	35.0	29.0	35.0
100-101	32.161436298140046	33.5	30.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	15.0
20	65.0
21	469.0
22	1922.0
23	6232.0
24	15708.0
25	32272.0
26	56923.0
27	88953.0
28	126736.0
29	168345.0
30	214173.0
31	264975.0
32	327351.0
33	408237.0
34	519972.0
35	675086.0
36	932191.0
37	1369038.0
38	1589562.0
39	767902.0
40	26753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.09384657894064	23.222815789841064	15.953059720933368	23.730277910284922
2	13.193134414026932	23.43279825482546	42.13231237338812	21.24175495775949
3	16.133134146805634	29.024337659358114	32.40487177268503	22.437656421151228
4	11.410343080487346	20.346790540562786	40.895190930770305	27.347675448179565
5	11.646394610109837	39.328413111121705	37.01415351904587	12.011038759722593
6	26.837319478954104	41.06722585705928	19.885887861815842	12.20956680217077
7	23.655862996967027	34.098207492463665	25.660029266880578	16.585900243688727
8	22.76546639339318	38.24421346202931	22.592975895055396	16.39734424952212
9	24.839566846949552	16.87178860411633	22.632078307024923	35.656566241909196
10-11	22.725922778728815	27.996372128527746	30.592428540309204	18.685276552434242
12-13	23.710848698709043	25.748664901065904	30.45727163186895	20.083214768356104
14-15	20.937642650050403	27.021314172931966	28.16142803220174	23.879615144815894
16-17	20.22567978799091	30.048873405381514	28.86626579999531	20.859181006632262
18-19	20.26563148224975	29.270056698077795	30.54895724266909	19.915354577003367
20-21	21.230901609083432	28.476116894407994	30.250943732458747	20.042037764049827
22-23	20.884064192734698	28.418941253786663	30.124208138865676	20.572786414612963
24-25	20.65092124910595	28.589707741844688	30.17799738675912	20.58137362229024
26-27	20.604964541812183	28.75377554881417	30.040191219764296	20.60106868960935
28-29	20.52975392809264	28.80952625953445	30.09692958258566	20.56379022978725
30-31	20.848301303539404	28.639536842244322	29.99805146123894	20.514110392977333
32-33	20.652991143434292	28.857503619666105	29.872003678517352	20.617501558382255
34-35	20.78955451310772	28.919251511815297	29.914835734686392	20.376358240390598
36-37	20.770110540541463	28.840975036814413	29.85659881954474	20.532315603099384
38-39	20.602344866057436	28.942619654669393	29.86562537872441	20.58941010054876
40-41	20.822652088605103	28.800344202594264	29.801045106683695	20.57595860211694
42-43	20.742231248836436	28.910300754650354	29.53424102546754	20.813226971045673
44-45	20.838347524265263	29.055429422929073	29.35354476220927	20.752678290596393
46-47	20.899137908393094	29.003369237314164	29.135391542342155	20.96210131195059
48-49	20.807398577927103	29.13120393944788	29.134779594624487	20.92661788800053
50-51	20.8569557154069	29.17866971598217	28.986182393725613	20.97819217488532
52-53	20.93403916592069	29.106157199828026	28.929360027071706	21.03044360717958
54-55	20.91249597042603	29.0357059714487	28.995151443338152	21.056646614787116
56-57	20.944033109133386	29.19091323173868	28.854115629411364	21.01093802971657
58-59	20.90038295019392	29.232243243936185	28.92512670709374	20.942247098776157
60-61	21.01816564180036	29.224241337328362	28.84553990188626	20.91205311898502
62-63	20.92785001277152	29.2561969593003	28.881131830720847	20.934821197207334
64-65	20.871003426202577	29.19952228341171	28.882639856504188	21.04683443388152
66-67	20.968840898608214	29.278839921180477	28.722945660860365	21.029373519350937
68-69	20.919886103408846	29.215649047123776	28.82764536070856	21.036819488758816
70-71	20.96653757279439	28.947439980927737	28.897234854225335	21.18878759205254
72-73	20.990100965867843	29.055665398488394	28.840003753500387	21.11422988214338
74-75	20.85215319840973	29.137252738788465	28.805027268758813	21.20556679404299
76-77	20.94975241661742	29.083661798552185	28.77835987613257	21.188225908697824
78-79	20.94856181271175	29.051502598923605	28.76864104932909	21.231294539035556
80-81	20.950578004187467	29.059113507279015	28.77246992860572	21.217838559927795
82-83	20.975176458753193	28.990190910602216	28.893185834757283	21.141446795887305
84-85	21.05224346477775	28.999912104951196	28.81062673677236	21.137217693498698
86-87	20.904784173169613	29.046124070157582	28.876640188037193	21.172451568635612
88-89	20.98381596072999	28.94975251724899	28.875882644586266	21.190548877434754
90-91	21.01547302825515	28.91642174350526	28.938717474924204	21.129387753315385
92-93	21.003813349815786	28.84713683787693	28.951419210471517	21.197630601835765
94-95	20.84200528013228	28.836182866146864	29.05765270428921	21.264159149431645
96-97	20.732379576029018	28.916501543424683	29.172855376603813	21.17826350394249
98-99	20.876173052940715	29.518972720857832	29.20604763699144	20.39880658921001
100-101	24.19218021548474	36.65792131609283	30.25897268697923	8.890925781443205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2457.0
1	2071.5
2	2456.5
3	3389.0
4	4364.5
5	6549.0
6	8457.5
7	10117.5
8	11370.0
9	11492.0
10	10908.0
11	10324.0
12	11403.5
13	14839.0
14	22640.5
15	33437.0
16	42205.0
17	47078.5
18	48637.0
19	48126.5
20	46680.0
21	45983.0
22	47560.0
23	53355.5
24	64116.0
25	79741.5
26	99173.0
27	120308.5
28	143398.0
29	167215.5
30	190277.5
31	213419.5
32	236552.5
33	257556.5
34	276571.0
35	294061.0
36	312299.5
37	326418.5
38	333789.0
39	338419.5
40	340671.5
41	340846.0
42	338166.5
43	334323.5
44	329316.5
45	322923.5
46	313675.0
47	303224.5
48	291428.0
49	276495.5
50	261533.5
51	244349.5
52	224636.0
53	204310.5
54	184759.0
55	166799.0
56	149544.5
57	132302.5
58	115422.5
59	99175.0
60	82613.0
61	66633.0
62	51720.5
63	39128.5
64	29393.0
65	22339.0
66	17137.5
67	13160.0
68	10194.5
69	7197.0
70	4780.5
71	3353.0
72	2318.5
73	1606.5
74	1121.5
75	797.5
76	569.5
77	376.0
78	255.5
79	174.5
80	130.0
81	104.5
82	84.5
83	59.0
84	36.0
85	23.5
86	18.0
87	15.5
88	14.0
89	13.5
90	9.0
91	5.0
92	5.5
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3170227962158774E-5
2	0.0038193661090260445
3	0.0
4	0.0
5	1.3170227962158774E-5
6	1.3170227962158774E-5
7	0.0
8	1.3170227962158774E-5
9	1.3170227962158774E-5
10-11	1.3170227962158774E-5
12-13	0.0
14-15	1.3170227962158774E-5
16-17	1.3170227962158774E-5
18-19	4.346175227512396E-4
20-21	5.268357625071798E-4
22-23	2.107629526677782E-4
24-25	1.1200344179988214E-4
26-27	0.0023994182333637263
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03317061548738845
38-39	0.01717824569608792
40-41	0.02175613776339969
42-43	0.01067635314713984
44-45	0.004091870112946018
46-47	0.005280613337621491
48-49	0.009482051131143306
50-51	0.007095107653701813
52-53	0.011513447379659232
54-55	0.0037073275586139496
56-57	0.017586653631153807
58-59	0.013847536517991085
60-61	0.014240190689168148
62-63	0.005260849153430778
64-65	0.006991284121188181
66-67	3.9475923949790415E-4
68-69	7.28377066239651E-4
70-71	0.0011039323938814545
72-73	9.054488346914655E-5
74-75	0.0038218947001252127
76-77	0.004578007556335725
78-79	0.0037099725522146436
80-81	5.847163183328289E-4
82-83	0.003548299362210931
84-85	0.004948922432987625
86-87	0.0016131459664293325
88-89	0.07485973597898701
90-91	0.0010372213050584903
92-93	9.721484334557106E-6
94-95	5.17403719874044E-4
96-97	0.011697135675414411
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	219.0
20-21	834.0
22-23	1682.0
24-25	3877.0
26-27	4605.0
28-29	7516.0
30-31	19683.0
32-33	25543.0
34-35	36610.0
36-37	56606.0
38-39	65362.0
40-41	65664.0
42-43	74446.0
44-45	87025.0
46-47	88480.0
48-49	80530.0
50-51	73498.0
52-53	70978.0
54-55	77135.0
56-57	81287.0
58-59	81551.0
60-61	79538.0
62-63	75560.0
64-65	80486.0
66-67	85751.0
68-69	86804.0
70-71	86567.0
72-73	82990.0
74-75	82692.0
76-77	84473.0
78-79	92068.0
80-81	93651.0
82-83	90118.0
84-85	90552.0
86-87	95667.0
88-89	101527.0
90-91	110329.0
92-93	114470.0
94-95	133024.0
96-97	178410.0
98-99	593990.0
100-101	4151085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.1802667045699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.35741019030961	82.36458472915173
2	5.597168223593332	9.983139099407943
3	1.207064737407239	3.229390656349776
4	0.4067477763830393	1.4509550071732082
5	0.17840542625197964	0.7955121747347009
6	0.08930872687242948	0.47787456489173097
7	0.04661340702056413	0.2909897249071806
8	0.03223913639858809	0.23000758258808746
9	0.020252896769559253	0.16255428619044746
>10	0.06338825497473166	0.8605406465066939
>50	9.743415410268894E-4	0.05979352548942487
>100	3.863996041093814E-4	0.07149811225987997
>500	4.0482873752753285E-5	0.02315989034914846
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3170227962158774E-5
2	0.0	3.9510683886476325E-5	0.0	0.0	1.3170227962158774E-5
3	0.0	3.9510683886476325E-5	0.0	0.0	1.3170227962158774E-5
4	0.0	5.2680911848635096E-5	0.0	0.0	1.3170227962158774E-5
5	0.0	5.2680911848635096E-5	0.0	0.0	1.3170227962158774E-5
6	0.0	5.2680911848635096E-5	0.0	0.0	1.3170227962158774E-5
7	0.0	5.2680911848635096E-5	0.0	0.0	2.6340455924317548E-5
8	0.0	7.902136777295265E-5	0.0	0.0	2.6340455924317548E-5
9	0.0	1.9755341943238162E-4	0.0	1.3170227962158774E-5	2.6340455924317548E-5
10-11	1.3170227962158774E-5	1.9755341943238162E-4	0.0	1.3170227962158774E-5	2.6340455924317548E-5
12-13	1.3170227962158774E-5	1.9755341943238162E-4	0.0	1.3170227962158774E-5	2.6340455924317548E-5
14-15	1.3170227962158774E-5	2.4364921729993735E-4	0.0	1.3170227962158774E-5	3.9510683886476325E-5
16-17	1.3170227962158774E-5	2.5023433128101673E-4	0.0	1.3170227962158774E-5	3.9510683886476325E-5
18-19	1.3170227962158774E-5	2.634045592431755E-4	0.0	1.3170227962158774E-5	3.9510683886476325E-5
20-21	1.3170227962158774E-5	2.7657478720533426E-4	0.0	1.975534194323816E-5	5.2680911848635096E-5
22-23	1.3170227962158774E-5	3.2925569905396936E-4	0.0	2.6340455924317548E-5	5.2680911848635096E-5
24-25	1.3170227962158774E-5	3.490110409972075E-4	0.0	3.9510683886476325E-5	5.2680911848635096E-5
26-27	1.3170227962158774E-5	3.6876638294044566E-4	0.0	1.448725075837465E-4	5.2680911848635096E-5
28-29	1.3170227962158774E-5	3.6876638294044566E-4	0.0	4.543728646944777E-4	5.2680911848635096E-5
30-31	1.3170227962158774E-5	3.819366109026045E-4	0.0	0.0014092143919509888	5.2680911848635096E-5
32-33	1.3170227962158774E-5	3.819366109026045E-4	0.0	0.003918142818742236	7.243625379187325E-5
34-35	2.6340455924317548E-5	4.2144729478908077E-4	0.0	0.0071975295813197695	7.902136777295265E-5
36-37	3.9510683886476325E-5	4.609579786755571E-4	0.0	0.012077099041299597	7.902136777295265E-5
38-39	5.2680911848635096E-5	4.609579786755571E-4	0.0	0.019702661031389526	8.560648175403203E-5
40-41	5.2680911848635096E-5	5.136388905241922E-4	0.0	0.028665001159638572	9.219159573511141E-5
42-43	5.2680911848635096E-5	5.333942324674304E-4	0.0	0.03663957419072571	9.219159573511141E-5
44-45	5.2680911848635096E-5	5.992453722782243E-4	0.0	0.04537143532963697	9.219159573511141E-5
46-47	5.2680911848635096E-5	6.4534117014578E-4	0.0	0.05583518144557212	9.219159573511141E-5
48-49	5.2680911848635096E-5	6.519262841268593E-4	0.0	0.06550871388377774	9.219159573511141E-5
50-51	5.2680911848635096E-5	7.441178798619707E-4	0.0	0.07496493756060774	9.219159573511141E-5
52-53	5.2680911848635096E-5	7.572881078241295E-4	0.0	0.08475700205047279	1.0536182369727019E-4
54-55	5.2680911848635096E-5	7.704583357862883E-4	0.0	0.09514831191261607	1.1853205165942897E-4
56-57	5.2680911848635096E-5	7.770434497673677E-4	0.0	0.10550669620485394	1.2511716564050836E-4
58-59	5.2680911848635096E-5	7.770434497673677E-4	0.0	0.1148180473741002	1.3170227962158775E-4
60-61	5.2680911848635096E-5	8.16554133653844E-4	0.0	0.1237935577303114	1.3170227962158775E-4
62-63	5.2680911848635096E-5	8.297243616160028E-4	0.0	0.13304564287372794	1.448725075837465E-4
64-65	5.2680911848635096E-5	9.35086185313273E-4	0.0	0.14189603606429863	1.448725075837465E-4
66-67	5.2680911848635096E-5	9.416712992943524E-4	0.0	0.15099007847216928	1.448725075837465E-4
68-69	6.585113981079387E-5	9.745968691997494E-4	0.0	0.1597087693831184	1.514576215648259E-4
70-71	6.585113981079387E-5	9.943522111429876E-4	0.0	0.16767017218624336	1.580427355459053E-4
72-73	6.585113981079387E-5	0.0010075224391051462	0.0	0.1739787113801174	1.8438319147022283E-4
74-75	6.585113981079387E-5	0.0010338628950294637	0.0	0.1798262925953159	1.8438319147022283E-4
76-77	6.585113981079387E-5	0.0010470331229916227	0.0	0.18467293648539035	1.9755341943238162E-4
78-79	6.585113981079387E-5	0.001053618236972702	0.0	0.18930227161408913	1.9755341943238162E-4
80-81	6.585113981079387E-5	0.001053618236972702	0.0	0.1918968065226344	2.1730876137561976E-4
82-83	6.585113981079387E-5	0.001053618236972702	0.0	0.1936286914996583	2.3706410331885794E-4
84-85	6.585113981079387E-5	0.0010667884649348607	0.0	0.1951300974873444	2.3706410331885794E-4
86-87	6.585113981079387E-5	0.0010799586928970195	0.0	0.19564373637786858	2.3706410331885794E-4
88-89	6.585113981079387E-5	0.001106299148821337	0.0	0.19569641728971723	2.3706410331885794E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9335	0.0	49.556923	1
GTATCAA	17135	0.0	35.54039	1
ATCAACG	21620	0.0	27.480194	3
TCAACGC	21685	0.0	27.32177	4
CAACGCA	22070	0.0	26.845156	5
TATCAAC	22350	0.0	26.841778	2
AACGCAG	22750	0.0	26.133368	6
ACGCAGA	26225	0.0	22.529015	7
CGCAGAG	26315	0.0	22.389292	8
GTACATG	24080	0.0	20.375875	1
TACATGG	24050	0.0	20.195564	2
ACATGGG	24005	0.0	19.683142	3
GCAGAGT	30470	0.0	19.06558	9
AGAGTAC	28350	0.0	18.33891	10-11
CATGGGG	15760	0.0	18.181934	4
GAGTACT	17615	0.0	17.62496	12-13
TGGTATC	4125	0.0	17.492018	2
GTGGTAT	4460	0.0	17.195066	1
CAGAGTA	30585	0.0	16.762888	10-11
ATGGGGG	9400	0.0	16.22876	5
>>END_MODULE