FastQCFastQC Report
Fri 27 May 2016
SRR2139847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14947848
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250000.051.7605321
GTATCAA457500.035.982291
CTTATAC143650.030.1556111
ATCAACG558800.029.856133
TCAACGC559400.029.838354
CAACGCA571750.029.1938325
TATCAAC577200.029.0594982
AACGCAG589850.028.3518666
TGGTATC111150.025.508932
GTGGTAT115100.025.1521151
ACGCAGA673600.024.7825497
CGCAGAG680100.024.595818
TATACAC224850.022.5459753
GCAGAGT779500.021.4156829
TTATACA226300.020.3102042
GAGTACT475150.020.18946512-13
CAGAGTA775450.019.3434610-11
ACATCTC236650.018.9045528
ACACATC238200.018.7457646
GTACTTT524900.018.13849314-15
Produced by FastQC (version 0.11.5)