##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14947848
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.164139547043828	32.0	25.0	33.0	18.0	33.0
2	30.139121765219983	32.0	29.0	33.0	25.0	33.0
3	31.617162350058685	33.0	32.0	33.0	27.0	34.0
4	31.77539368877714	33.0	32.0	33.0	30.0	34.0
5	32.17239986652259	33.0	33.0	34.0	31.0	34.0
6	35.64326791388299	38.0	36.0	38.0	30.0	38.0
7	35.99711570521723	38.0	37.0	38.0	31.0	38.0
8	36.35506375232074	38.0	37.0	38.0	33.0	38.0
9	36.480403399874014	38.0	38.0	38.0	34.0	38.0
10-11	36.588983544654724	38.0	38.0	38.0	34.0	38.0
12-13	36.613503595969135	38.0	38.0	38.0	34.0	38.0
14-15	36.63507740378414	38.0	38.0	38.0	34.0	38.0
16-17	36.643517414680694	38.0	38.0	38.0	34.0	38.0
18-19	36.65926091836096	38.0	38.0	38.0	34.0	38.0
20-21	36.65428680864601	38.0	38.0	38.0	34.0	38.0
22-23	36.65290893955973	38.0	38.0	38.0	34.0	38.0
24-25	36.65626043898848	38.0	38.0	38.0	34.0	38.0
26-27	36.60294146408857	38.0	38.0	38.0	34.0	38.0
28-29	36.57991312662499	38.0	38.0	38.0	34.0	38.0
30-31	36.57713268749026	38.0	38.0	38.0	34.0	38.0
32-33	36.55132233374553	38.0	38.0	38.0	34.0	38.0
34-35	36.511923815032574	38.0	38.0	38.0	34.0	38.0
36-37	36.492505012849705	38.0	38.0	38.0	34.0	38.0
38-39	36.4650191596022	38.0	38.0	38.0	34.0	38.0
40-41	36.48174983265032	38.0	38.0	38.0	34.0	38.0
42-43	36.43366764549546	38.0	38.0	38.0	34.0	38.0
44-45	36.3811784160446	38.0	38.0	38.0	34.0	38.0
46-47	36.337719460142694	38.0	38.0	38.0	33.0	38.0
48-49	36.28780474295704	38.0	38.0	38.0	33.0	38.0
50-51	36.26297076070853	38.0	38.0	38.0	33.0	38.0
52-53	36.24803749436742	38.0	38.0	38.0	33.0	38.0
54-55	36.21657176139824	38.0	37.0	38.0	33.0	38.0
56-57	36.219258366925914	38.0	37.0	38.0	33.0	38.0
58-59	36.199687112670304	38.0	37.0	38.0	33.0	38.0
60-61	36.18812821379948	38.0	37.0	38.0	33.0	38.0
62-63	36.188763734411495	38.0	37.0	38.0	33.0	38.0
64-65	36.18327035797597	38.0	37.0	38.0	33.0	38.0
66-67	36.18581513410496	38.0	37.0	38.0	33.0	38.0
68-69	36.17014985126029	38.0	37.0	38.0	33.0	38.0
70-71	36.14247713810755	38.0	37.0	38.0	33.0	38.0
72-73	36.11777811585125	38.0	37.0	38.0	33.0	38.0
74-75	36.142470222147196	38.0	37.0	38.0	33.0	38.0
76-77	36.12992650767478	38.0	37.0	38.0	33.0	38.0
78-79	36.12129535251321	38.0	37.0	38.0	33.0	38.0
80-81	36.08629737896789	38.0	37.0	38.0	33.0	38.0
82-83	36.09257899579776	38.0	37.0	38.0	33.0	38.0
84-85	36.09340893054591	38.0	37.0	38.0	33.0	38.0
86-87	36.09362561535692	38.0	37.0	38.0	33.0	38.0
88-89	36.06827491247394	38.0	37.0	38.0	33.0	38.0
90-91	36.054757359424414	38.0	37.0	38.0	33.0	38.0
92-93	36.0094915298478	38.0	37.0	38.0	31.0	38.0
94-95	36.003410773911774	38.0	37.0	38.0	31.5	38.0
96-97	36.03362113180952	38.0	37.0	38.0	32.5	38.0
98-99	36.061978789231134	38.0	37.0	38.0	33.0	38.0
100-101	35.536897446304955	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	50.0
19	111.0
20	218.0
21	754.0
22	3216.0
23	10101.0
24	23535.0
25	45999.0
26	77670.0
27	120101.0
28	172006.0
29	232550.0
30	299096.0
31	378051.0
32	474760.0
33	610906.0
34	822659.0
35	1231270.0
36	2483123.0
37	7961665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.55766716386198	23.998491287842903	14.904071810203048	23.539769738092065
2	14.583925391802218	21.758115281878705	42.10488359260811	21.553075733710966
3	17.59847823589792	27.86922184202204	30.836435714625477	23.69586420745456
4	12.480478795342313	19.521887030159792	39.56050396016872	28.43713021432918
5	12.82328220934454	37.942493277058034	36.31300984317954	12.921214670417877
6	27.828112782522275	39.785312240263615	19.172940479458983	13.213634497755129
7	24.863170939388734	33.926274872476625	23.718885822226717	17.491668365907923
8	23.018718145916388	38.227790381598744	21.56886395954789	17.184627512936977
9	24.590041322336166	17.784660373854482	22.06450052208184	35.56079778172751
10-11	23.14366589759275	27.966540735495837	29.33142282420854	19.558370542702868
12-13	24.231916192886093	25.352291513801855	29.63334922859799	20.782443064714066
14-15	21.391141526640645	26.830951869889752	27.45037554406248	24.32753105940712
16-17	20.51874692995095	30.07270779287787	28.22362550862256	21.184919768548617
18-19	20.51513033849421	28.814378497827914	30.357523705084503	20.31296745859337
20-21	21.413581554579313	27.818132986572348	30.372230514037106	20.396054944811233
22-23	21.063043207655223	27.91040979953326	30.244291505524977	20.78225548728654
24-25	20.905885842711186	28.115866008686396	30.353093953295655	20.62515419530676
26-27	20.88362631570511	28.239297178281213	30.231439213680172	20.645637292333504
28-29	20.637171374377367	28.307805584681766	30.32202615255841	20.73299688838246
30-31	21.306428812763777	28.03210207312065	30.242394573018945	20.419074541096627
32-33	20.62316363584588	28.385219869389626	30.230942982815513	20.760673511948976
34-35	20.88704000120687	28.36640114404808	30.292166922239794	20.45439193250526
36-37	20.77074036100347	28.266178216638053	30.25180142658893	20.711279995769544
38-39	20.644633969994413	28.12845847543668	30.41974309692725	20.80716445764166
40-41	20.916416266608767	28.01887949084827	30.367456234499112	20.697248008043854
42-43	21.039364100366168	27.99185485429254	30.10381487981665	20.864966165524642
44-45	20.878559657986244	28.310402802442674	29.913985293575486	20.897052245995592
46-47	21.117716967649823	28.11864170795122	29.525024607758095	21.238616716640855
48-49	21.044401947724683	28.239525408348037	29.48059150109502	21.235481142832256
50-51	20.935497079856752	28.353320015239763	29.454674733855196	21.25650817104829
52-53	21.055117003053248	28.387002682057588	29.52814782480119	21.029732490087973
54-55	21.076976051962017	28.334727860626156	29.334994668746827	21.253301418664996
56-57	21.264131412779754	28.554194781715385	29.021898400382018	21.159775405122847
58-59	20.95890328791025	28.832409268800106	29.324051440740163	20.88463600254948
60-61	21.17369857123946	29.040189818704835	28.945678126994146	20.84043348306156
62-63	20.923717202876723	29.25222032752228	28.916568129403604	20.907494340197392
64-65	20.878939127582203	29.152342554830135	28.981188042965258	20.987530274622408
66-67	21.04054146888811	29.101605082784605	28.79520555339285	21.06264789493444
68-69	21.043929146886548	28.93599944196531	28.947960110178357	21.072111300969787
70-71	21.094674069613102	28.525073056378492	29.09411431680991	21.2861385571985
72-73	21.164897801377546	28.440276173491508	29.142430769859466	21.252395255271477
74-75	21.07031246819314	28.575839026975768	29.056584440146626	21.29726406468447
76-77	21.095140306488737	28.5706662788867	29.01845437506021	21.31573903956435
78-79	21.15536630237159	28.585201458827996	28.943799663822677	21.315632574977734
80-81	21.147011922665854	28.57891089540577	28.919519192030002	21.354557989898375
82-83	21.234186049876058	28.556347531581928	29.00007680611088	21.209389612431135
84-85	21.370594119892143	28.54582318366058	28.849947570718314	21.233635125728963
86-87	21.14942934459324	28.62702625076578	28.923313366971108	21.300231037669874
88-89	21.279352332822658	28.58055491554185	28.834895755416973	21.30519699621852
90-91	21.32960685984954	28.565963248341035	28.892660883907773	21.211769007901648
92-93	21.317109229906205	28.604780035356164	28.808584788242058	21.26952594649557
94-95	21.160657802318468	28.720849701297897	28.818285343448373	21.30020715293526
96-97	21.19887637456406	28.703747485866888	28.880678120264903	21.216698019304157
98-99	21.414648748052787	29.25457285369109	28.88416055875121	20.446617839504913
100-101	21.92056534455305	31.827251734988437	26.402639774573196	19.84954314588532
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3686.0
1	3608.5
2	4844.5
3	6508.0
4	8205.0
5	11675.0
6	14880.5
7	18694.5
8	22653.0
9	24558.0
10	24059.5
11	23026.0
12	25109.0
13	32113.5
14	47222.5
15	65855.5
16	79980.5
17	87553.5
18	88307.5
19	83594.0
20	77090.5
21	71340.5
22	70489.5
23	78896.0
24	96727.0
25	123560.0
26	158237.5
27	195986.0
28	234407.5
29	274587.5
30	312603.5
31	349841.0
32	391922.0
33	436454.5
34	478537.0
35	517896.0
36	558985.5
37	592972.5
38	611201.0
39	622865.5
40	635714.0
41	641456.5
42	638750.5
43	644177.0
44	670545.0
45	680352.0
46	653168.0
47	622542.0
48	595745.0
49	567696.0
50	536702.5
51	501752.5
52	462791.5
53	421914.0
54	383176.5
55	345009.0
56	305959.0
57	266598.5
58	230391.0
59	198325.5
60	165448.0
61	132047.5
62	100778.5
63	75537.0
64	57245.5
65	42881.0
66	31296.5
67	23251.0
68	17507.5
69	12740.5
70	9102.5
71	6518.0
72	4656.0
73	3205.5
74	2183.0
75	1480.0
76	965.5
77	586.0
78	383.0
79	276.5
80	195.0
81	132.5
82	96.5
83	72.5
84	47.5
85	25.0
86	10.5
87	5.5
88	5.5
89	6.0
90	6.0
91	3.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003070676126757511
4	0.0
5	0.053251812568605186
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.0034889303129118E-5
16-17	3.34496310104304E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.503541993525211E-4
28-29	2.2764533673381903E-4
30-31	0.0
32-33	0.0
34-35	7.744974294598686E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.0434033898715371E-5
46-47	1.264456424934912E-4
48-49	0.0
50-51	2.1468479799055028E-5
52-53	1.803645615092214E-5
54-55	0.0011636582404112833
56-57	0.00209463325612904
58-59	0.00871522351845643
60-61	0.007963792138821566
62-63	0.00421301422346793
64-65	0.005257757066724364
66-67	0.006911629012078457
68-69	0.003723208428907691
70-71	0.005616144490777198
72-73	0.001077494093536544
74-75	0.0015559296863282032
76-77	0.0013563968039869757
78-79	0.0011574858336709333
80-81	6.668945778886602E-5
82-83	0.0
84-85	0.0
86-87	3.754170041464161E-4
88-89	8.517266245811798E-4
90-91	0.0
92-93	0.0
94-95	1.09008617494575E-4
96-97	3.2260437219253546E-4
98-99	9.51629482299763E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	224.0
20-21	886.0
22-23	1787.0
24-25	2291.0
26-27	4834.0
28-29	11189.0
30-31	28629.0
32-33	38015.0
34-35	52816.0
36-37	82724.0
38-39	100252.0
40-41	99033.0
42-43	115454.0
44-45	138353.0
46-47	139993.0
48-49	128144.0
50-51	115527.0
52-53	108924.0
54-55	117834.0
56-57	124479.0
58-59	125250.0
60-61	124103.0
62-63	121838.0
64-65	138208.0
66-67	152247.0
68-69	149175.0
70-71	164882.0
72-73	125550.0
74-75	127040.0
76-77	134701.0
78-79	141170.0
80-81	143612.0
82-83	134753.0
84-85	132904.0
86-87	137893.0
88-89	145065.0
90-91	149131.0
92-93	144138.0
94-95	151142.0
96-97	228662.0
98-99	1169864.0
100-101	9495132.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.58227312409369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33448183964414	72.94862305556786
2	7.878272359334355	13.012112794490955
3	2.0050974480188586	4.967565152781499
4	0.7848059232779042	2.5924422842216956
5	0.3720325038079815	1.5361644920255575
6	0.21443573823272027	1.0625154421380698
7	0.124380805632536	0.7190154763498628
8	0.07861840604778439	0.5193989344655223
9	0.04704770706113936	0.3496775934946826
>10	0.15744705852563992	1.9175958394671428
>50	0.0022970780672809045	0.12692947693290516
>100	9.696924736745299E-4	0.14659226924707497
>500	7.092636808116057E-5	0.038440844698892446
>1k	4.2513507987837915E-5	0.06292634411828325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.68992620208608E-6	1.337985240417216E-5
2	0.0	2.006977860625824E-5	0.0	6.68992620208608E-6	1.337985240417216E-5
3	0.0	2.675970480834432E-5	0.0	1.337985240417216E-5	1.337985240417216E-5
4	0.0	6.020933581877472E-5	0.0	2.006977860625824E-5	1.337985240417216E-5
5	0.0	6.689926202086079E-5	0.0	2.006977860625824E-5	1.337985240417216E-5
6	3.3449631010430395E-5	7.358918822294688E-5	0.0	2.006977860625824E-5	1.337985240417216E-5
7	3.3449631010430395E-5	7.358918822294688E-5	0.0	2.006977860625824E-5	1.337985240417216E-5
8	3.3449631010430395E-5	7.358918822294688E-5	0.0	3.3449631010430395E-5	2.006977860625824E-5
9	3.3449631010430395E-5	1.2041867163754943E-4	0.0	4.013955721251648E-5	2.006977860625824E-5
10-11	4.013955721251648E-5	1.271085978396355E-4	0.0	5.351940961668864E-5	2.006977860625824E-5
12-13	4.682948341460256E-5	1.3379852404172158E-4	0.0	6.689926202086079E-5	2.006977860625824E-5
14-15	4.682948341460256E-5	1.6055822885006592E-4	0.0	6.689926202086079E-5	3.34496310104304E-5
16-17	9.031400372816207E-5	1.6055822885006592E-4	0.0	8.696904062711903E-5	4.013955721251648E-5
18-19	9.365896682920512E-5	1.67248155052152E-4	0.0	1.1707370853650638E-4	4.348452031355952E-5
20-21	9.365896682920512E-5	1.8731793365841024E-4	0.0	1.438334133448507E-4	5.0174446515645595E-5
22-23	1.0034889303129119E-4	2.1407763846675453E-4	0.0	1.6055822885006592E-4	6.020933581877472E-5
24-25	1.1038378233442031E-4	2.2745749087092672E-4	0.0	2.0069778606258238E-4	7.024422512190383E-5
26-27	1.1707370853650638E-4	2.341474170730128E-4	0.0	2.776319373865723E-4	7.358918822294688E-5
28-29	1.2041867163754943E-4	2.341474170730128E-4	0.0	6.054383212887902E-4	7.358918822294688E-5
30-31	1.2041867163754943E-4	2.8097690048761536E-4	0.0	0.0016958962922288211	7.358918822294688E-5
32-33	1.2376363473859246E-4	2.8097690048761536E-4	0.0	0.003910261865119313	7.358918822294688E-5
34-35	1.3045356094067856E-4	3.110815683970027E-4	0.0	0.007004352733584126	7.358918822294688E-5
36-37	1.3379852404172158E-4	3.4787616250847613E-4	0.0	0.012132181167483106	7.358918822294688E-5
38-39	1.3379852404172158E-4	3.5456608871056223E-4	0.0	0.01948775502667675	7.358918822294688E-5
40-41	1.3379852404172158E-4	3.813257935189065E-4	0.0	0.027843472853082262	7.358918822294688E-5
42-43	1.3379852404172158E-4	3.8467075661994957E-4	0.0	0.03529605064220616	7.358918822294688E-5
44-45	1.3714348714276463E-4	4.4488009243872425E-4	0.0	0.04389929573808886	7.358918822294688E-5
46-47	1.4048845024380768E-4	5.017444651564559E-4	0.0	0.05335216146163649	8.027911442503296E-5
48-49	1.4717837644589375E-4	5.017444651564559E-4	0.0	0.06343388024818021	8.027911442503296E-5
50-51	1.5386830264797982E-4	5.652987640762737E-4	0.0	0.0733349710272676	8.027911442503296E-5
52-53	1.6055822885006592E-4	5.753336533794028E-4	0.0	0.08286142593903818	9.031400372816207E-5
54-55	1.6055822885006592E-4	5.820235795814889E-4	0.0	0.0929431447255819	1.0034889303129119E-4
56-57	1.6055822885006592E-4	6.087832843898332E-4	0.0	0.10057635052216213	1.0369385613233424E-4
58-59	1.6055822885006592E-4	6.154732105919193E-4	0.0	0.10724955190874298	1.1038378233442031E-4
60-61	1.67248155052152E-4	6.154732105919193E-4	0.0	0.11379564469748421	1.1707370853650638E-4
62-63	1.67248155052152E-4	6.221631367940054E-4	0.0	0.11999386132371698	1.2376363473859246E-4
64-65	1.67248155052152E-4	7.158221036232106E-4	0.0	0.12620545780235387	1.271085978396355E-4
66-67	1.8062800745632414E-4	7.292019560273827E-4	0.0	0.13243377909649603	1.271085978396355E-4
68-69	1.8731793365841024E-4	7.49271734633641E-4	0.0	0.13882600358258929	1.271085978396355E-4
70-71	1.906628967594533E-4	7.65996550138856E-4	0.0	0.14466965412011146	1.271085978396355E-4
72-73	2.2076756466884063E-4	7.693415132398991E-4	0.0	0.1494295366128957	1.4048845024380768E-4
74-75	2.2076756466884063E-4	7.827213656440713E-4	0.0	0.15321603484327645	1.4048845024380768E-4
76-77	2.2411252776988368E-4	8.094810704524156E-4	0.0	0.15663124216944138	1.4048845024380768E-4
78-79	2.3080245397196975E-4	8.228609228565878E-4	0.0	0.15959822444006655	1.4048845024380768E-4
80-81	2.341474170730128E-4	8.228609228565878E-4	0.0	0.1609930740532015	1.4717837644589375E-4
82-83	2.341474170730128E-4	8.262058859576308E-4	0.0	0.16150485340766108	1.5386830264797982E-4
84-85	2.341474170730128E-4	8.295508490586738E-4	0.0	0.16203670254072694	1.5386830264797982E-4
86-87	2.341474170730128E-4	8.696904062711903E-4	0.0	0.16222402047438533	1.5386830264797982E-4
88-89	2.4083734327509887E-4	9.365896682920512E-4	0.0	0.16226416003159785	1.6055822885006592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25000	0.0	51.760532	1
GTATCAA	45750	0.0	35.98229	1
CTTATAC	14365	0.0	30.155611	1
ATCAACG	55880	0.0	29.85613	3
TCAACGC	55940	0.0	29.83835	4
CAACGCA	57175	0.0	29.193832	5
TATCAAC	57720	0.0	29.059498	2
AACGCAG	58985	0.0	28.351866	6
TGGTATC	11115	0.0	25.50893	2
GTGGTAT	11510	0.0	25.152115	1
ACGCAGA	67360	0.0	24.782549	7
CGCAGAG	68010	0.0	24.59581	8
TATACAC	22485	0.0	22.545975	3
GCAGAGT	77950	0.0	21.415682	9
TTATACA	22630	0.0	20.310204	2
GAGTACT	47515	0.0	20.189465	12-13
CAGAGTA	77545	0.0	19.34346	10-11
ACATCTC	23665	0.0	18.904552	8
ACACATC	23820	0.0	18.745764	6
GTACTTT	52490	0.0	18.138493	14-15
>>END_MODULE