FastQCFastQC Report
Fri 27 May 2016
SRR2139846_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139846_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9778078
Sequences flagged as poor quality0
Sequence length19-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120700.047.596361
GTATCAA222900.035.101911
ATCAACG276950.027.582673
TCAACGC280850.027.1703854
CAACGCA284200.026.8501175
AACGCAG294700.025.9631776
TATCAAC298450.025.899482
ACGCAGA337700.022.63297
CGCAGAG340650.022.4127738
GTACATG328150.020.1617071
TACATGG324700.020.09752
ACATGGG325500.019.6181623
GCAGAGT399350.018.8919729
AGAGTAC369400.018.1098910-11
CATGGGG211450.017.5873014
GAGTACT223350.017.43226212-13
GTGGTAT59200.016.728971
CAGAGTA397300.016.5071710-11
ACCGAAC9550.016.350788
AGTACTT251100.016.19308512-13
Produced by FastQC (version 0.11.5)