##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9778078
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84822068304221	31.0	31.0	34.0	30.0	34.0
2	32.010471076217634	31.0	31.0	34.0	30.0	34.0
3	32.03448550931993	33.0	31.0	34.0	30.0	34.0
4	35.745778669386766	37.0	35.0	37.0	35.0	37.0
5	35.561194541503966	37.0	35.0	37.0	33.0	37.0
6	35.57092692449375	37.0	35.0	37.0	33.0	37.0
7	35.46478725164598	37.0	35.0	37.0	33.0	37.0
8	35.44665904690063	37.0	35.0	37.0	33.0	37.0
9	36.98842758259855	39.0	37.0	39.0	33.0	39.0
10-11	36.8799439930833	39.0	37.0	39.0	33.0	39.0
12-13	36.821732655435966	39.0	37.0	39.0	32.0	39.0
14-15	37.940281362042725	40.0	37.0	41.0	33.0	41.0
16-17	37.92757078640608	40.0	37.0	41.0	33.0	41.0
18-19	37.97299556211354	40.0	37.0	41.0	33.0	41.0
20-21	37.95604954142267	40.0	37.0	41.0	33.0	41.0
22-23	37.889526032281026	40.0	37.0	41.0	32.5	41.0
24-25	37.80374865322257	40.0	37.0	41.0	32.0	41.0
26-27	37.61681324721534	39.0	37.0	41.0	32.0	41.0
28-29	37.51591468694514	39.0	36.0	41.0	32.0	41.0
30-31	37.369717547758356	39.0	36.0	41.0	31.5	41.0
32-33	37.52766622442661	39.0	36.0	41.0	32.0	41.0
34-35	37.48743962017406	39.0	36.0	41.0	32.0	41.0
36-37	37.40471055623796	39.0	36.0	41.0	31.5	41.0
38-39	37.30294691621589	39.0	36.0	41.0	31.0	41.0
40-41	37.222539810285724	39.0	36.0	41.0	31.0	41.0
42-43	37.18798081573908	39.0	36.0	40.5	31.0	41.0
44-45	37.21871377431431	39.0	36.0	40.5	31.0	41.0
46-47	37.331123447840206	39.0	36.0	41.0	31.0	41.0
48-49	37.28463364011668	39.0	36.0	41.0	31.0	41.0
50-51	37.18977504444318	39.0	36.0	41.0	31.0	41.0
52-53	37.10962851021657	39.0	36.0	41.0	31.0	41.0
54-55	37.00205148613057	39.0	35.0	40.0	31.0	41.0
56-57	36.89244902336331	39.0	35.0	40.0	31.0	41.0
58-59	36.77421012661169	39.0	35.0	40.0	30.5	41.0
60-61	36.581310755166754	38.0	35.0	40.0	30.0	41.0
62-63	36.3887873942731	38.0	35.0	40.0	30.0	41.0
64-65	36.21043191694331	38.0	34.0	40.0	30.0	41.0
66-67	35.97411210232637	37.5	34.0	40.0	29.0	41.0
68-69	35.71455952032747	37.0	34.0	40.0	29.0	41.0
70-71	35.43709642404829	36.5	34.0	39.0	29.0	41.0
72-73	35.141191079799924	36.0	34.0	39.0	29.0	40.0
74-75	34.792576985131845	36.0	33.5	39.0	29.0	40.0
76-77	33.72638896100821	35.0	31.5	37.0	27.5	39.0
78-79	34.13575688074074	35.0	33.0	37.0	28.5	39.0
80-81	34.0138979995299	35.0	33.0	37.0	29.0	39.0
82-83	33.75532387767885	35.0	33.0	36.5	29.0	39.0
84-85	33.50421692132812	35.0	33.0	36.0	29.0	37.5
86-87	33.268480262672625	35.0	32.0	36.0	28.0	37.0
88-89	33.03969505164821	34.5	32.0	35.0	28.0	37.0
90-91	32.89066528327879	34.0	32.0	35.0	28.0	36.0
92-93	32.796025151308754	34.0	32.0	35.0	28.0	36.0
94-95	32.74219772968628	34.0	32.0	35.0	28.0	36.0
96-97	32.73921816747558	34.0	32.0	35.0	29.0	35.0
98-99	32.84064494329641	34.0	32.0	35.0	29.0	35.0
100-101	32.19107114749467	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	24.0
20	112.0
21	510.0
22	2451.0
23	7634.0
24	19304.0
25	40479.0
26	71307.0
27	111615.0
28	158075.0
29	211456.0
30	270309.0
31	337251.0
32	413433.0
33	517827.0
34	662651.0
35	864533.0
36	1197972.0
37	1766807.0
38	2065039.0
39	1023319.0
40	35965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.239117953446474	23.083145787955463	15.78382786473988	23.89390839385818
2	13.228735209804945	23.364077172931772	42.18322000205161	21.22396761521167
3	16.24577958981305	29.01630565843308	32.2401293996632	22.497785352090666
4	11.385223149171035	20.284835117903537	40.80752884155762	27.522412891367814
5	11.68507452184848	39.36014175391631	36.88377422461197	12.071009499623239
6	26.846605232643878	40.92594679649723	19.964731310181815	12.262716660677079
7	23.735022363290618	33.99003362419486	25.581796340753264	16.69314767176126
8	22.782462974830022	38.28662442659999	22.569005892569074	16.361906706000916
9	24.906755703932816	16.82773444842637	22.590707498958384	35.674802348682434
10-11	22.79535405628795	28.03047285979924	30.4789857475058	18.695187336407013
12-13	23.75269454794695	25.74704353964041	30.363364865774233	20.136897046638406
14-15	20.96587386600925	26.98792134814224	28.107635263289982	23.938569522558524
16-17	20.241595536464324	29.983515165250267	28.82274512434857	20.95214417393684
18-19	20.298488061929756	29.22456593065731	30.499692883424768	19.977253123988167
20-21	21.32948239661031	28.396534934241345	30.147487590046346	20.126495079101996
22-23	20.974242623857656	28.39854183305402	29.97432895434549	20.65288658874283
24-25	20.701466046058954	28.58164139082785	30.117893091996045	20.598999471117153
26-27	20.65960008713876	28.702460646284607	29.994104415822235	20.6438348507544
28-29	20.561633544297056	28.79576947125766	30.051308190872046	20.59128879357324
30-31	20.900947087483658	28.59055152759669	29.90526355631858	20.603237828601078
32-33	20.759981123656765	28.75921475440823	29.835973835433776	20.64483028650123
34-35	20.82561858381645	28.85672123227277	29.87793611152256	20.439724072388223
36-37	20.754227640140012	28.790350615799582	29.836789767725826	20.618631976334584
38-39	20.59445923423617	28.862439092012654	29.91523714773149	20.62786452601969
40-41	20.82522452163822	28.744485479785975	29.79863100015087	20.631658998424935
42-43	20.796266868839545	28.842562225562503	29.549307020571586	20.81186388502637
44-45	20.877094610623477	29.01154327244495	29.308228557872717	20.803133559058853
46-47	20.915404755463143	28.942172517017383	29.087544294456734	21.054878433062736
48-49	20.881449361730557	29.044146518269876	29.086475129424677	20.98792899057489
50-51	20.858863168734928	29.113134035628864	28.95043194682898	21.077570848807227
52-53	20.94828739657202	29.062921059331416	28.91351833657014	21.075273207526426
54-55	20.963093888136953	29.025198306667203	28.90732563316474	21.104382172031105
56-57	21.033936071664357	29.10502875982408	28.791283120375823	21.069752048135744
58-59	20.925837302071837	29.17879396440826	28.91842276656365	20.976945966956254
60-61	21.055504460246606	29.19904038143568	28.785174313337798	20.960280844979916
62-63	21.004337515619355	29.17814940077869	28.79950534836076	21.018007735241195
64-65	20.90512318046658	29.163668166965955	28.836006598859054	21.095202053708416
66-67	21.08727718168292	29.162869742333626	28.65549789299986	21.094355182983595
68-69	20.997972072208558	29.13193023048022	28.77040097046408	21.099696726847135
70-71	20.99169851620167	28.89424195209988	28.86980175658593	21.244257775112523
72-73	21.059431474668077	28.95734322699704	28.832906726996395	21.150318571338488
74-75	20.90116888041083	29.032050073059107	28.819705531673844	21.24707551485622
76-77	20.968190524374744	28.990746133342622	28.769960740068345	21.271102602214288
78-79	21.021587933710983	29.00103860976172	28.734614672955544	21.24275878357175
80-81	21.01460017165421	28.961135431675366	28.765046043605413	21.259218353065005
82-83	21.058487834232892	28.875003013039326	28.913896401323086	21.1526127514047
84-85	21.16434618314016	28.84437032529134	28.78832201105136	21.20296148051714
86-87	20.92095525993674	28.97699604638061	28.864566902557698	21.23748179112495
88-89	21.046325492780323	28.85064081714982	28.836413121916415	21.266620568153442
90-91	21.06354579322316	28.810735588201545	28.94604051254881	21.179678106026483
92-93	21.056059843431782	28.75965459158094	28.945049277360162	21.239236287627115
94-95	20.888833780125292	28.786626237157492	29.021435869760133	21.30310411295708
96-97	20.814105132208923	28.856039795001575	29.105581316236805	21.22427375655269
98-99	20.916700363147132	29.487299451897726	29.191270150825193	20.40473003412995
100-101	24.231086922732874	36.63652991312201	30.190278113747492	8.942105050397618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2989.0
1	2593.5
2	3244.5
3	4501.0
4	5843.5
5	8872.0
6	11522.0
7	13701.5
8	15103.5
9	14984.5
10	14009.0
11	13007.5
12	14206.0
13	18641.5
14	29294.5
15	43847.5
16	55391.0
17	61343.0
18	62843.5
19	61793.5
20	59114.0
21	57433.0
22	59300.0
23	66894.0
24	80933.5
25	100936.0
26	126848.5
27	155146.0
28	184710.0
29	215352.5
30	244270.5
31	272232.0
32	300721.5
33	327511.0
34	353636.5
35	378399.5
36	400933.5
37	418319.5
38	427503.5
39	431934.0
40	435751.0
41	436425.5
42	431959.5
43	427570.0
44	422281.5
45	415640.5
46	405850.0
47	393194.5
48	380921.5
49	364041.0
50	342724.5
51	320060.0
52	295893.0
53	271559.5
54	247379.0
55	223395.5
56	199716.0
57	175628.0
58	152837.0
59	131792.5
60	110656.5
61	89039.0
62	68588.0
63	52173.5
64	38990.5
65	29259.5
66	22090.5
67	16524.5
68	12559.5
69	8795.5
70	5878.0
71	4156.0
72	2848.0
73	1953.0
74	1361.5
75	959.0
76	665.0
77	439.0
78	289.0
79	197.0
80	142.0
81	111.0
82	95.0
83	71.5
84	44.5
85	31.5
86	28.5
87	22.0
88	21.0
89	21.0
90	15.0
91	10.5
92	9.5
93	6.0
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0041010104439747775
3	0.0
4	0.0
5	6.136175227892435E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.2726267882092984E-4
20-21	5.522834183500359E-4
22-23	2.9151797758124467E-4
24-25	1.8928833673310534E-4
26-27	0.0024517412609371845
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03403014577725346
38-39	0.01727814063966807
40-41	0.021696267882784382
42-43	0.010882066972110985
44-45	0.004485554358449275
46-47	0.004953066283877103
48-49	0.008504816723411525
50-51	0.006181435710886924
52-53	0.010285612633197693
54-55	0.0038586247895548917
56-57	0.015244056770371571
58-59	0.012120945832143354
60-61	0.011932683525236321
62-63	0.004392304010407898
64-65	0.0058947500589475
66-67	4.0692176386527336E-4
68-69	7.94082522056111E-4
70-71	0.001052953474235846
72-73	5.791032894418081E-5
74-75	0.004137343084411457
76-77	0.00530931914602051
78-79	0.0042408268093376686
80-81	7.650877350697911E-4
82-83	0.0033342032362193384
84-85	0.004441020912336789
86-87	0.0015726619364251054
88-89	0.07239608667667004
90-91	9.772714268438854E-4
92-93	7.622822731257385E-6
94-95	3.979676183893189E-4
96-97	0.011950685804029942
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	296.0
20-21	979.0
22-23	2039.0
24-25	4755.0
26-27	5876.0
28-29	9527.0
30-31	25858.0
32-33	33578.0
34-35	47484.0
36-37	73565.0
38-39	85919.0
40-41	87049.0
42-43	99105.0
44-45	115868.0
46-47	116980.0
48-49	106326.0
50-51	97238.0
52-53	93939.0
54-55	102171.0
56-57	107722.0
58-59	108217.0
60-61	107136.0
62-63	101257.0
64-65	107255.0
66-67	113099.0
68-69	113261.0
70-71	113781.0
72-73	108729.0
74-75	109253.0
76-77	110779.0
78-79	118920.0
80-81	122632.0
82-83	116843.0
84-85	117654.0
86-87	124140.0
88-89	131676.0
90-91	142091.0
92-93	147191.0
94-95	170253.0
96-97	227071.0
98-99	757342.0
100-101	5293224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.85019673517105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.35100255938325	80.25203546796932
2	6.255267513229694	10.990529633367055
3	1.3977194313664443	3.6836978107834066
4	0.49207367328484236	1.7291507602508676
5	0.20935506607657464	0.9195941871165909
6	0.10814471515829716	0.5700320701515265
7	0.057172175390211334	0.35158107990654397
8	0.034139116874414024	0.23993025070258156
9	0.020288070410262236	0.16040798792266858
>10	0.07310098229404581	0.9589326505370482
>50	0.0014421830530049185	0.08207918372342082
>100	2.613542614962644E-4	0.040473162260041946
>500	3.315921750877877E-5	0.021555755309017464
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0226958713154058E-5
2	0.0	0.0	0.0	0.0	1.0226958713154058E-5
3	0.0	0.0	0.0	0.0	1.0226958713154058E-5
4	0.0	3.068087613946217E-5	0.0	1.0226958713154058E-5	1.0226958713154058E-5
5	0.0	3.068087613946217E-5	0.0	1.0226958713154058E-5	1.0226958713154058E-5
6	0.0	3.068087613946217E-5	0.0	1.0226958713154058E-5	2.0453917426308115E-5
7	0.0	3.068087613946217E-5	0.0	2.0453917426308115E-5	2.0453917426308115E-5
8	0.0	4.090783485261623E-5	0.0	5.113479356577029E-5	2.0453917426308115E-5
9	1.0226958713154058E-5	7.15887109920784E-5	0.0	1.3295046327100276E-4	2.0453917426308115E-5
10-11	1.0226958713154058E-5	7.15887109920784E-5	0.0	1.380639426275798E-4	2.0453917426308115E-5
12-13	1.0226958713154058E-5	7.15887109920784E-5	0.0	1.5340438069731086E-4	2.5567396782885144E-5
14-15	1.0226958713154058E-5	1.073830664881176E-4	0.0	1.9942569490650412E-4	4.602131420919326E-5
16-17	2.5567396782885144E-5	1.1249654584469463E-4	0.0	2.3522005040254332E-4	5.113479356577029E-5
18-19	4.090783485261623E-5	1.227235045578487E-4	0.0	2.710144058985825E-4	5.113479356577029E-5
20-21	4.090783485261623E-5	1.3295046327100276E-4	0.0	3.426031168906609E-4	5.113479356577029E-5
22-23	4.090783485261623E-5	1.4829090134073383E-4	0.0	3.937379104564312E-4	5.6248272922347314E-5
24-25	4.090783485261623E-5	1.73858298123619E-4	0.0	4.3464574530904744E-4	6.136175227892435E-5
26-27	5.113479356577029E-5	1.943122155499271E-4	0.0	5.21574894370857E-4	6.136175227892435E-5
28-29	7.15887109920784E-5	1.943122155499271E-4	0.0	0.0010124689126022517	7.15887109920784E-5
30-31	1.1249654584469463E-4	2.2499309168938925E-4	0.0	0.0023726544214517416	7.15887109920784E-5
32-33	1.1249654584469463E-4	2.2499309168938925E-4	0.0	0.005998111285264855	7.15887109920784E-5
34-35	1.227235045578487E-4	2.6590092654200546E-4	0.0	0.010636037061680221	8.181566970523246E-5
36-37	1.380639426275798E-4	3.170357201077758E-4	0.0	0.016669942702441112	8.181566970523246E-5
38-39	1.4829090134073383E-4	3.374896375340839E-4	0.0	0.025807730312644266	1.0226958713154058E-4
40-41	1.5340438069731086E-4	3.7328399303012305E-4	0.0	0.0361062777367904	1.1249654584469463E-4
42-43	1.5340438069731086E-4	4.0907834852616225E-4	0.0	0.044973050941094966	1.1249654584469463E-4
44-45	1.6363133941046492E-4	4.653266214485096E-4	0.0	0.05553749929178311	1.1761002520127166E-4
46-47	1.6363133941046492E-4	5.011209769445488E-4	0.0	0.06684340214917492	1.227235045578487E-4
48-49	1.6363133941046492E-4	5.062344563011258E-4	0.0	0.07772999969932742	1.431774219841568E-4
50-51	1.6363133941046492E-4	5.727096879366272E-4	0.0	0.08846830634813918	1.431774219841568E-4
52-53	1.73858298123619E-4	5.931636053629353E-4	0.0	0.0984140236966815	1.585178600538879E-4
54-55	1.73858298123619E-4	5.982770847195124E-4	0.0	0.10854893978141716	1.73858298123619E-4
56-57	1.73858298123619E-4	6.136175227892434E-4	0.0	0.11897021071012115	1.78971777480196E-4
58-59	1.73858298123619E-4	6.187310021458205E-4	0.0	0.12931989292783305	1.8919873619335006E-4
60-61	1.73858298123619E-4	6.391849195721286E-4	0.0	0.13988434127852117	2.1476613297623522E-4
62-63	1.73858298123619E-4	6.442983989287056E-4	0.0	0.14983517210642008	2.1476613297623522E-4
64-65	1.73858298123619E-4	6.852062337813218E-4	0.0	0.15907522930375478	2.1476613297623522E-4
66-67	1.73858298123619E-4	6.852062337813218E-4	0.0	0.1684431234850039	2.1476613297623522E-4
68-69	1.73858298123619E-4	6.95433192494476E-4	0.0	0.17768829416169518	2.1476613297623522E-4
70-71	1.73858298123619E-4	7.0566015120763E-4	0.0	0.18591076896707104	2.3522005040254332E-4
72-73	1.73858298123619E-4	7.15887109920784E-4	0.0	0.19246624950220279	2.659009265420055E-4
74-75	1.73858298123619E-4	7.465679860602461E-4	0.0	0.19843367991132818	2.659009265420055E-4
76-77	1.73858298123619E-4	7.721353828431313E-4	0.0	0.20373124452474198	2.659009265420055E-4
78-79	1.73858298123619E-4	7.874758209128625E-4	0.0	0.208445872491506	2.659009265420055E-4
80-81	1.73858298123619E-4	7.874758209128625E-4	0.0	0.21110999523628263	2.710144058985825E-4
82-83	1.78971777480196E-4	7.977027796260165E-4	0.0	0.21252642901805446	2.761278852551596E-4
84-85	1.8408525683677302E-4	8.079297383391705E-4	0.0	0.21388661452690394	2.761278852551596E-4
86-87	1.8408525683677302E-4	8.079297383391705E-4	0.0	0.21437239506577876	2.761278852551596E-4
88-89	1.8408525683677302E-4	8.283836557654786E-4	0.0	0.21446955117355374	3.068087613946217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12070	0.0	47.59636	1
GTATCAA	22290	0.0	35.10191	1
ATCAACG	27695	0.0	27.58267	3
TCAACGC	28085	0.0	27.170385	4
CAACGCA	28420	0.0	26.850117	5
AACGCAG	29470	0.0	25.963177	6
TATCAAC	29845	0.0	25.89948	2
ACGCAGA	33770	0.0	22.6329	7
CGCAGAG	34065	0.0	22.412773	8
GTACATG	32815	0.0	20.161707	1
TACATGG	32470	0.0	20.0975	2
ACATGGG	32550	0.0	19.618162	3
GCAGAGT	39935	0.0	18.891972	9
AGAGTAC	36940	0.0	18.10989	10-11
CATGGGG	21145	0.0	17.587301	4
GAGTACT	22335	0.0	17.432262	12-13
GTGGTAT	5920	0.0	16.72897	1
CAGAGTA	39730	0.0	16.50717	10-11
ACCGAAC	955	0.0	16.35078	8
AGTACTT	25110	0.0	16.193085	12-13
>>END_MODULE