FastQCFastQC Report
Fri 27 May 2016
SRR2139845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7083230
Sequences flagged as poor quality0
Sequence length19-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86600.048.904891
GTATCAA157200.034.866681
ATCAACG194000.027.8059333
TCAACGC195250.027.5858024
CAACGCA198850.027.065715
TATCAAC205050.026.4896352
AACGCAG204350.026.4579686
ACGCAGA236550.022.8216557
CGCAGAG238700.022.61618
GCAGAGT279500.019.0646389
GTACATG203650.018.7166581
TACATGG199550.018.3594192
AGAGTAC259050.018.0539910-11
ACATGGG198250.017.9186763
GAGTACT171900.017.50816212-13
CAGAGTA276950.017.03557210-11
TATACTG47050.016.0791685
CATGGGG132700.016.0186164
AGTACTT189700.016.0170512-13
GTACTTT197700.015.23453814-15
Produced by FastQC (version 0.11.5)