FastQCFastQC Report
Fri 27 May 2016
SRR2139844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6069472
Sequences flagged as poor quality0
Sequence length19-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75650.046.65611
GTATCAA135250.033.7574121
ACCGAGG53950.028.91273792-93
GACCGAG54900.028.19611790-91
AGACCGA55400.027.47594890-91
GAGACCG56250.027.34081588-89
CGAGACC57200.027.10576488-89
ATCAACG167850.026.5112883
TCAACGC168950.026.3386784
ACGAGAC61450.026.07474386-87
CAACGCA172150.025.8251065
AACGCAG177000.025.1874316
TATCAAC179200.025.0174522
GCCCACG62750.024.93393582-83
CACGAGA63350.024.66521686-87
CCCACGA62750.024.43837784-85
CCACGAG65400.024.17946484-85
CGAGCCC64450.023.2110580-81
CCGAGCC66100.023.18437278-79
CTCCGAG66450.022.79526176-77
Produced by FastQC (version 0.11.5)