##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139844_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6069472
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.762602908457275	31.0	31.0	34.0	30.0	34.0
2	31.936270733269716	31.0	31.0	34.0	30.0	34.0
3	31.959918095017162	31.0	31.0	34.0	30.0	34.0
4	35.68867473150877	37.0	35.0	37.0	33.0	37.0
5	35.49700188088848	37.0	35.0	37.0	33.0	37.0
6	35.5058098958196	37.0	35.0	37.0	33.0	37.0
7	35.396534657380414	37.0	35.0	37.0	33.0	37.0
8	35.380484496839266	37.0	35.0	37.0	33.0	37.0
9	36.90568981947688	39.0	37.0	39.0	33.0	39.0
10-11	36.792134142805175	39.0	37.0	39.0	32.0	39.0
12-13	36.73615736261738	39.0	35.0	39.0	32.0	39.0
14-15	37.82944974455768	40.0	37.0	41.0	32.0	41.0
16-17	37.814278490781405	40.0	37.0	41.0	32.0	41.0
18-19	37.860968878347244	40.0	37.0	41.0	32.5	41.0
20-21	37.84449625102157	40.0	37.0	41.0	32.0	41.0
22-23	37.778060509315495	39.5	37.0	41.0	32.0	41.0
24-25	37.69182628614537	39.0	37.0	41.0	32.0	41.0
26-27	37.4987796688786	39.0	36.0	41.0	32.0	41.0
28-29	37.39259773606119	39.0	36.0	41.0	32.0	41.0
30-31	37.24191787661577	39.0	36.0	40.0	31.0	41.0
32-33	37.4040735064864	39.0	36.0	40.5	32.0	41.0
34-35	37.360524517496884	39.0	36.0	41.0	31.5	41.0
36-37	37.27340775843428	39.0	36.0	41.0	31.0	41.0
38-39	37.169795973395125	39.0	36.0	40.5	31.0	41.0
40-41	37.076517294618995	39.0	36.0	40.0	31.0	41.0
42-43	37.04034220413901	39.0	35.5	40.0	31.0	41.0
44-45	37.073001455213614	39.0	35.5	40.0	31.0	41.0
46-47	37.187735371509774	39.0	36.0	41.0	31.0	41.0
48-49	37.14048626812442	39.0	36.0	41.0	31.0	41.0
50-51	37.04458122757514	39.0	36.0	40.5	31.0	41.0
52-53	36.961567421886144	39.0	35.0	40.0	31.0	41.0
54-55	36.85215253927015	39.0	35.0	40.0	30.0	41.0
56-57	36.739689931928865	39.0	35.0	40.0	30.0	41.0
58-59	36.61702396393139	38.5	35.0	40.0	30.0	41.0
60-61	36.42668799113876	38.0	35.0	40.0	30.0	41.0
62-63	36.23484773248572	38.0	34.0	40.0	29.0	41.0
64-65	36.062736933012026	38.0	34.0	40.0	29.0	41.0
66-67	35.84041888584575	37.5	34.0	40.0	29.0	41.0
68-69	35.57960381378608	37.0	34.0	40.0	29.0	41.0
70-71	35.30563890103329	36.5	34.0	39.0	29.0	41.0
72-73	35.0102412724359	36.0	33.5	39.0	28.0	40.0
74-75	34.6673494696579	36.0	33.0	39.0	28.0	40.0
76-77	33.602359482513876	35.0	31.5	37.0	27.0	39.0
78-79	34.015568828418196	35.0	32.5	37.0	28.0	39.0
80-81	33.88360484432165	35.0	33.0	37.0	28.0	39.0
82-83	33.616259199193614	35.0	32.5	36.0	28.0	38.5
84-85	33.36979151987938	35.0	32.0	36.0	28.0	37.0
86-87	33.13599438406233	35.0	32.0	35.5	28.0	37.0
88-89	32.90751346555293	34.0	32.0	35.0	27.0	36.5
90-91	32.75240390154856	34.0	32.0	35.0	27.0	36.0
92-93	32.66361756985198	34.0	32.0	35.0	27.0	36.0
94-95	32.604588026001956	34.0	32.0	35.0	27.0	36.0
96-97	32.60282419442576	34.0	32.0	35.0	27.5	35.0
98-99	32.719392068202055	34.0	32.0	35.0	29.0	35.0
100-101	32.08246347933972	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	61.0
21	351.0
22	1544.0
23	4983.0
24	12749.0
25	26616.0
26	47050.0
27	73747.0
28	105143.0
29	139993.0
30	177680.0
31	219725.0
32	269253.0
33	334364.0
34	427024.0
35	551057.0
36	754951.0
37	1092219.0
38	1237424.0
39	574911.0
40	18617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.59145912386763	23.498555310668756	16.068352579656448	23.841632985807166
2	13.14516489123932	23.743669515292105	41.97002822263008	21.141137370838507
3	16.113279705384585	29.05796418535253	32.50345334816603	22.325302761096847
4	11.32642367020124	20.55121443038446	41.00841737278257	27.113944526631727
5	11.508169328101246	39.388918737728545	37.17007627188597	11.93283566228424
6	26.651395706249243	41.38552743961913	19.9050922386659	12.057984615465728
7	23.44164368828129	34.11814075425342	25.76054391551687	16.679671641948428
8	22.545140664624533	38.53036969278382	22.629068887705554	16.29542075488609
9	24.681076047471677	16.833128153486825	22.72111972837176	35.764676070669736
10-11	22.50743557264948	28.13488553864323	30.765007236214288	18.592671652493
12-13	23.463828484586468	25.892861850256498	30.607884837429022	20.03542482772801
14-15	20.69875270863759	27.25384514501426	28.299768085263434	23.74763406108472
16-17	20.040664163208923	30.194405707778206	29.01949296413263	20.74543716488024
18-19	20.04858266376404	29.43955868152117	30.731076247613252	19.780782407101537
20-21	20.983876130057013	28.660869823116496	30.459041959629158	19.896212087197334
22-23	20.657020833973615	28.58415280559888	30.313471572819044	20.445354787608462
24-25	20.402982081580376	28.753229213295516	30.38780066826572	20.455988036858386
26-27	20.415717495247993	28.909344415482625	30.23040630213261	20.444531787136768
28-29	20.305326680470138	29.014022305377512	30.270684327978632	20.40996668617372
30-31	20.69064645240381	28.825701230581647	30.160388931425185	20.323263385589353
32-33	20.497136735308118	28.999968841528013	30.028488456117604	20.47440596704627
34-35	20.525485613870288	29.10793259650115	30.12002659696817	20.246555192660395
36-37	20.54243380527894	29.025169530439744	30.055511903639108	20.37688476064221
38-39	20.349332962867273	29.114237747944237	30.105529686756267	20.43089960243222
40-41	20.62238313494617	28.974089798180746	29.96395711125064	20.439569955622446
42-43	20.530585325667218	29.079380405500356	29.70853937825431	20.68149489057812
44-45	20.584934487434587	29.22736613838039	29.536820210169157	20.650879164015866
46-47	20.635408454711033	29.148219830323807	29.31583487765323	20.90053683731193
48-49	20.58633945552834	29.297985618139165	29.33095301661034	20.784721909722155
50-51	20.626802236749885	29.38482183335823	29.148779013971275	20.839596915920605
52-53	20.687445878005274	29.27463578680318	29.097247399645443	20.9406709355461
54-55	20.690612340629635	29.226916938426236	29.12961295790979	20.952857763034345
56-57	20.726154415944425	29.29205297650741	29.02969952746134	20.95209308008683
58-59	20.666790419921448	29.364817337568223	29.085696814035643	20.882695428474683
60-61	20.684967140852436	29.153284525263157	29.167954547130186	20.993793786754217
62-63	20.839024601203022	29.194702021224522	29.20286338546018	20.763409992112276
64-65	20.489934202715137	29.198210955341867	29.17336096079415	21.138493881148847
66-67	20.67062091963759	29.30351282577645	28.911878498469807	21.113987756116153
68-69	20.61518119442933	29.550118235942236	28.976240253284963	20.85846031634347
70-71	20.62128090587729	29.26232864711695	29.074950681372126	21.041439765633637
72-73	20.63238749129678	29.31459407447538	28.787657431453056	21.265361002774785
74-75	20.581233548204732	29.332232476792118	28.74842171881187	21.33811225619128
76-77	20.663157853672608	29.06141905861343	28.907589109345682	21.367833978368285
78-79	20.72561754245954	29.029134186291046	28.890356630054388	21.354891641195028
80-81	20.980913943644325	29.01321347387038	28.65532882706513	21.350543755420166
82-83	20.988378844938822	29.256900804638587	28.761957459028814	20.992762891393774
84-85	20.822315020650052	28.97456086565447	28.655727586793688	21.547396526901792
86-87	20.623586360555237	29.33970494982879	28.752217089225873	21.28449160039011
88-89	21.056658559013467	28.91964645682949	28.742027454899578	21.28166752925747
90-91	21.117674062593395	29.140738432614523	28.82120269077969	20.920384814012394
92-93	20.810133791209292	29.043078591155265	28.855044413521863	21.291743204113583
94-95	20.933762992446223	28.784662835376107	28.95694373827865	21.324630433899017
96-97	20.763957923672262	29.180815676336575	29.11859127867157	20.9366351213196
98-99	20.948617189047482	29.5727714343545	29.265764239689396	20.21284713690862
100-101	23.979827510954724	36.84316344888113	30.37706497991801	8.799944060246139
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2129.0
1	1754.5
2	2120.0
3	2986.5
4	3862.0
5	5650.0
6	7154.5
7	8599.5
8	9700.5
9	9780.5
10	9187.5
11	8600.0
12	9451.0
13	12205.5
14	19160.5
15	28957.0
16	36397.0
17	39997.0
18	40770.5
19	40157.0
20	39399.5
21	39568.0
22	41471.5
23	47005.0
24	56918.0
25	70196.5
26	86937.5
27	104971.5
28	122830.0
29	140747.0
30	157652.0
31	173501.5
32	189322.5
33	205958.0
34	221913.5
35	235879.5
36	249959.5
37	260992.0
38	266074.5
39	268135.5
40	269477.5
41	269416.5
42	266896.0
43	263989.5
44	260503.0
45	255397.5
46	248367.0
47	240062.0
48	230704.5
49	218310.0
50	205124.0
51	191219.5
52	174367.5
53	158085.5
54	143166.0
55	128535.5
56	114917.0
57	101593.0
58	88281.0
59	75200.5
60	62150.5
61	49459.5
62	37978.0
63	28748.0
64	21741.5
65	16629.5
66	12779.0
67	9643.0
68	7284.5
69	5158.5
70	3448.0
71	2497.0
72	1780.0
73	1229.0
74	848.5
75	574.5
76	385.5
77	267.5
78	192.0
79	139.5
80	100.0
81	74.5
82	66.0
83	51.0
84	33.5
85	23.5
86	17.5
87	14.0
88	10.0
89	6.0
90	3.5
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.6475897738715988E-5
2	0.003772980582165961
3	0.0
4	1.6475897738715988E-5
5	1.6475897738715988E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.789456479904677E-4
20-21	5.354959681272799E-4
22-23	2.6366521175653516E-4
24-25	1.7308709907670397E-4
26-27	0.002622411142113655
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03249335555218501
38-39	0.016369692943017784
40-41	0.021635852444506887
42-43	0.009930888909832163
44-45	0.003930495307483168
46-47	0.0050076419251378615
48-49	0.009150350394394331
50-51	0.006852560505995945
52-53	0.0109299715902509
54-55	0.0036336265712796872
56-57	0.016854652783948952
58-59	0.01336206427378119
60-61	0.013061418912405323
62-63	0.005022861674503447
64-65	0.006739840416847509
66-67	3.6306826438945014E-4
68-69	5.473625881054725E-4
70-71	0.0010704312454437245
72-73	1.0245097567137661E-4
74-75	0.0028888855635529885
76-77	0.003890407330919102
78-79	0.003094080606259935
80-81	6.748798088043731E-4
82-83	0.0035978884712140136
84-85	0.0049292983312637035
86-87	0.0015683803530710375
88-89	0.07876167473002871
90-91	9.759628677074001E-4
92-93	0.0
94-95	4.7377647226038756E-4
96-97	0.011709039609678137
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	198.0
20-21	689.0
22-23	1377.0
24-25	3161.0
26-27	3675.0
28-29	5689.0
30-31	16282.0
32-33	21189.0
34-35	30169.0
36-37	45339.0
38-39	49853.0
40-41	52514.0
42-43	59813.0
44-45	69942.0
46-47	70575.0
48-49	64050.0
50-51	58824.0
52-53	52801.0
54-55	57827.0
56-57	61244.0
58-59	37149.0
60-61	65495.0
62-63	62341.0
64-65	66296.0
66-67	70830.0
68-69	72691.0
70-71	71952.0
72-73	68864.0
74-75	69049.0
76-77	70641.0
78-79	75953.0
80-81	77688.0
82-83	74354.0
84-85	74098.0
86-87	79201.0
88-89	84258.0
90-91	91460.0
92-93	95195.0
94-95	111034.0
96-97	146156.0
98-99	500338.0
100-101	3279218.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.5240028660743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.63963898698678	82.93471306183092
2	5.447756004030273	9.754098482369592
3	1.1429520113503393	3.069649174197395
4	0.3785981509772071	1.3557448781269568
5	0.15910716465491445	0.7121955132289758
6	0.08340331912296645	0.4479959388122734
7	0.0453183782449318	0.28399578367195794
8	0.02838895802613532	0.2033194527757281
9	0.017283907946372683	0.1392592162075229
>10	0.054762941589482333	0.7626998708695246
>50	0.0017661708670130738	0.10143235098957296
>100	9.473120604284081E-4	0.1892375991607942
>500	7.669414318021364E-5	0.045658677758732086
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6475897738715988E-5
2	0.0	3.2951795477431976E-5	0.0	0.0	1.6475897738715988E-5
3	0.0	3.2951795477431976E-5	0.0	0.0	1.6475897738715988E-5
4	0.0	6.590359095486395E-5	0.0	1.6475897738715988E-5	1.6475897738715988E-5
5	0.0	6.590359095486395E-5	0.0	4.942769321614796E-5	1.6475897738715988E-5
6	0.0	6.590359095486395E-5	0.0	4.942769321614796E-5	3.2951795477431976E-5
7	0.0	6.590359095486395E-5	0.0	6.590359095486395E-5	3.2951795477431976E-5
8	0.0	6.590359095486395E-5	0.0	6.590359095486395E-5	3.2951795477431976E-5
9	0.0	1.8123487512587585E-4	0.0	9.885538643229593E-5	3.2951795477431976E-5
10-11	0.0	1.8123487512587585E-4	0.0	1.318071819097279E-4	3.2951795477431976E-5
12-13	0.0	1.8123487512587585E-4	0.0	1.4828307964844388E-4	4.118974434678997E-5
14-15	0.0	2.389005172113818E-4	0.0	1.6475897738715985E-4	7.414153982422195E-5
16-17	0.0	2.471384660807398E-4	0.0	2.0594872173394985E-4	8.237948869357994E-5
18-19	0.0	2.471384660807398E-4	0.0	2.306625683420238E-4	8.237948869357994E-5
20-21	0.0	2.471384660807398E-4	0.0	2.965661592968878E-4	8.237948869357994E-5
22-23	0.0	2.800902615581718E-4	0.0	4.778010344227636E-4	8.237948869357994E-5
24-25	0.0	3.2951795477431976E-4	0.0	0.001194502586056909	8.237948869357994E-5
26-27	0.0	3.2951795477431976E-4	0.0	0.0031386585192253952	8.237948869357994E-5
28-29	0.0	3.2951795477431976E-4	0.0	0.015166063868488067	9.061743756293793E-5
30-31	0.0	3.954215457291837E-4	0.0	0.023181588118373394	9.885538643229593E-5
32-33	0.0	4.1189744346789965E-4	0.0	0.03196324161310902	9.885538643229593E-5
34-35	0.0	4.613251366840476E-4	0.0	0.04288676181387771	1.318071819097279E-4
36-37	0.0	5.107528299001956E-4	0.0	0.057336124130731636	1.318071819097279E-4
38-39	0.0	5.272287276389116E-4	0.0	0.08602066209383617	1.6475897738715988E-4
40-41	0.0	5.601805231163436E-4	0.0	0.14368630417934214	1.6475897738715988E-4
42-43	0.0	5.684184719857016E-4	0.0	0.16731274153666087	1.6475897738715988E-4
44-45	0.0	5.848943697244175E-4	0.0	0.20367504784600704	1.6475897738715988E-4
46-47	0.0	5.931323185937755E-4	0.0	0.245647397335386	1.7299692625651785E-4
48-49	0.0	5.931323185937755E-4	0.0	0.2808975805473689	1.9771077286459185E-4
50-51	0.0	6.425600118099235E-4	0.0	0.31517568579276745	1.9771077286459185E-4
52-53	0.0	6.425600118099235E-4	0.0	0.35820249273742427	2.0594872173394985E-4
54-55	0.0	6.507979606792815E-4	0.0	0.4427567999325147	2.1418667060330783E-4
56-57	0.0	6.755118072873555E-4	0.0	0.5334730928818849	2.1418667060330783E-4
58-59	0.0	7.084636027647874E-4	0.0	0.6105967701968145	2.1418667060330783E-4
60-61	0.0	7.084636027647874E-4	0.0	1.085563950208519	2.1418667060330783E-4
62-63	0.0	7.084636027647874E-4	0.0	1.0970806027278814	2.1418667060330783E-4
64-65	0.0	7.578912959809354E-4	0.0	1.1086054931961131	2.1418667060330783E-4
66-67	0.0	7.661292448502934E-4	0.0	1.1172635774578086	2.1418667060330783E-4
68-69	0.0	8.155569380664414E-4	0.0	1.125929899668373	2.1418667060330783E-4
70-71	0.0	8.320328358051573E-4	0.0	1.1342667039241634	2.1418667060330783E-4
72-73	0.0	8.402707846745153E-4	0.0	1.1405357830137448	2.471384660807398E-4
74-75	0.0	8.485087335438733E-4	0.0	1.146294109273426	2.471384660807398E-4
76-77	0.0	9.308882222374532E-4	0.0	1.151352209879212	2.553764149500978E-4
78-79	0.0	9.556020688455272E-4	0.0	1.1560396027858766	2.636143638194558E-4
80-81	0.0	9.556020688455272E-4	0.0	1.1585027494978146	2.718523126888138E-4
82-83	0.0	9.556020688455272E-4	0.0	1.1599279146522137	2.800902615581718E-4
84-85	0.0	9.720779665842432E-4	0.0	1.1612542244201802	2.883282104275298E-4
86-87	0.0	9.720779665842432E-4	0.0	1.1616743598125174	2.9656615929688775E-4
88-89	0.0	9.720779665842432E-4	0.0	1.1617649772500804	2.9656615929688775E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7565	0.0	46.6561	1
GTATCAA	13525	0.0	33.757412	1
ACCGAGG	5395	0.0	28.912737	92-93
GACCGAG	5490	0.0	28.196117	90-91
AGACCGA	5540	0.0	27.475948	90-91
GAGACCG	5625	0.0	27.340815	88-89
CGAGACC	5720	0.0	27.105764	88-89
ATCAACG	16785	0.0	26.511288	3
TCAACGC	16895	0.0	26.338678	4
ACGAGAC	6145	0.0	26.074743	86-87
CAACGCA	17215	0.0	25.825106	5
AACGCAG	17700	0.0	25.187431	6
TATCAAC	17920	0.0	25.017452	2
GCCCACG	6275	0.0	24.933935	82-83
CACGAGA	6335	0.0	24.665216	86-87
CCCACGA	6275	0.0	24.438377	84-85
CCACGAG	6540	0.0	24.179464	84-85
CGAGCCC	6445	0.0	23.21105	80-81
CCGAGCC	6610	0.0	23.184372	78-79
CTCCGAG	6645	0.0	22.795261	76-77
>>END_MODULE