FastQCFastQC Report
Fri 27 May 2016
SRR2139843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7802020
Sequences flagged as poor quality0
Sequence length19-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81300.039.7594071
GTATCAA135150.031.6317211
ATCAACG149350.027.417293
TCAACGC151450.027.1200584
CAACGCA153500.026.7578685
AACGCAG159000.025.9639436
TATCAAC170500.024.459532
ACGCAGA178300.023.0360777
CGCAGAG179600.022.8926498
TGCGCGA1850.005400952419.36790794-95
GCAGAGT223150.018.33119
TACATGG167750.017.5221632
GTACATG175000.017.418331
AGAGTAC204300.017.13281810-11
ACATGGG165300.016.7931253
GAGTACT140250.016.67300812-13
CAGAGTA224750.016.1048310-11
CATGGGG107150.014.3795914
AGTACTT162000.014.36988912-13
GTACTTT168850.013.94872814-15
Produced by FastQC (version 0.11.5)