FastQCFastQC Report
Fri 27 May 2016
SRR2139842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344532
Sequences flagged as poor quality0
Sequence length19-101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177200.050.2995381
CTTATAC136100.046.3553921
GTATCAA320950.037.5794941
TATACAC195550.036.6870353
TTATACA202500.033.8838772
ATCAACG370300.032.3514983
TCAACGC377350.031.8378834
TATCAAC378650.031.4910352
CAACGCA382150.031.4716035
AACGCAG399550.030.1603346
GTGGTAT82600.027.326641
TGGTATC77700.027.1757492
ACGCAGA455550.026.37767
CGCAGAG459050.026.2039538
ACACATC280900.024.6273966
TACACAT299300.024.3704515
ACATCTC287050.023.9501618
CACATCT302500.022.897187
GCAGAGT530500.022.706979
ATACACA358050.021.8669474
Produced by FastQC (version 0.11.5)