##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139842_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10344532
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.420382961742494	30.0	18.0	32.0	18.0	33.0
2	27.916009250104306	30.0	25.0	32.0	18.0	33.0
3	27.57433096054998	29.0	25.0	31.0	18.0	33.0
4	30.81635602267942	32.0	30.0	33.0	27.0	33.0
5	31.3144004001341	33.0	32.0	33.0	28.0	33.0
6	35.157989070941056	37.0	35.0	38.0	29.0	38.0
7	35.64347173946584	38.0	36.0	38.0	31.0	38.0
8	36.122257149960966	38.0	37.0	38.0	33.0	38.0
9	36.60211423774415	38.0	38.0	38.0	34.0	38.0
10-11	36.69495642722165	38.0	38.0	38.0	34.0	38.0
12-13	36.79310103154014	38.0	38.0	38.0	34.5	38.0
14-15	36.80021184138634	38.0	38.0	38.0	35.0	38.0
16-17	36.820376794232935	38.0	38.0	38.0	35.0	38.0
18-19	36.83713666311826	38.0	38.0	38.0	35.0	38.0
20-21	36.87831271058511	38.0	38.0	38.0	35.0	38.0
22-23	36.8666745267834	38.0	38.0	38.0	35.0	38.0
24-25	36.87257158032227	38.0	38.0	38.0	35.0	38.0
26-27	36.82760335291162	38.0	38.0	38.0	35.0	38.0
28-29	36.729056406291505	38.0	38.0	38.0	34.5	38.0
30-31	36.765340346023606	38.0	38.0	38.0	35.0	38.0
32-33	36.74765093801082	38.0	38.0	38.0	35.0	38.0
34-35	36.710884969689864	38.0	38.0	38.0	34.5	38.0
36-37	36.682369452242256	38.0	38.0	38.0	34.0	38.0
38-39	36.633062716151585	38.0	38.0	38.0	34.0	38.0
40-41	36.70887339132742	38.0	38.0	38.0	34.0	38.0
42-43	36.68887992369821	38.0	38.0	38.0	34.0	38.0
44-45	36.620260502844545	38.0	38.0	38.0	34.0	38.0
46-47	36.496518479442365	38.0	38.0	38.0	34.0	38.0
48-49	36.442209845309414	38.0	38.0	38.0	34.0	38.0
50-51	36.45204507106983	38.0	38.0	38.0	34.0	38.0
52-53	36.461282500263465	38.0	38.0	38.0	34.0	38.0
54-55	36.388578076354335	38.0	38.0	38.0	34.0	38.0
56-57	36.40081118575178	38.0	38.0	38.0	34.0	38.0
58-59	36.340436788859236	38.0	38.0	38.0	33.5	38.0
60-61	36.35061981013982	38.0	38.0	38.0	34.0	38.0
62-63	36.36668998719051	38.0	38.0	38.0	34.0	38.0
64-65	36.35772290410729	38.0	38.0	38.0	34.0	38.0
66-67	36.33059841635442	38.0	38.0	38.0	33.5	38.0
68-69	36.30225903588927	38.0	38.0	38.0	33.0	38.0
70-71	36.30060945219847	38.0	38.0	38.0	33.0	38.0
72-73	36.29248908569829	38.0	38.0	38.0	33.0	38.0
74-75	36.26809764913345	38.0	38.0	38.0	33.0	38.0
76-77	36.268534062202605	38.0	38.0	38.0	33.0	38.0
78-79	36.26530911638467	38.0	37.5	38.0	33.0	38.0
80-81	36.279518068817026	38.0	37.5	38.0	33.0	38.0
82-83	36.255843018300126	38.0	37.0	38.0	33.0	38.0
84-85	36.235384804845594	38.0	37.0	38.0	33.0	38.0
86-87	36.24359485337136	38.0	37.0	38.0	33.0	38.0
88-89	36.25549782737016	38.0	37.0	38.0	33.0	38.0
90-91	36.20942932480148	38.0	37.0	38.0	33.0	38.0
92-93	36.234568390761595	38.0	37.0	38.0	33.0	38.0
94-95	36.19715512207807	38.0	37.0	38.0	33.0	38.0
96-97	36.23046412370998	38.0	37.0	38.0	33.0	38.0
98-99	36.27533805483238	38.0	37.0	38.0	33.0	38.0
100-101	35.714128039969935	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	58.0
19	222.0
20	489.0
21	1103.0
22	2867.0
23	6884.0
24	15210.0
25	28426.0
26	48194.0
27	73894.0
28	105561.0
29	142167.0
30	182516.0
31	230532.0
32	290692.0
33	384370.0
34	548269.0
35	913734.0
36	2161539.0
37	5207802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.814603502604086	20.402788642347474	13.430428752117543	21.3521791029309
2	13.85424686201367	21.331955858418727	41.97200027995466	22.841796999612935
3	19.208070505267905	27.625986366517115	30.120183300704177	23.045759827510807
4	11.930167551320833	19.608542948100506	40.1454700898987	28.31581941067996
5	12.639141126390541	39.31982627705571	35.252525174371904	12.788507422181844
6	27.41873677803887	39.200932434642766	19.757955217307078	13.622375570011286
7	24.775736265809925	34.1705775183884	23.18314812011337	17.87053809568831
8	22.92174261725905	37.62715413321743	22.15858581132525	17.292517438198267
9	24.591416992088188	17.920801057022203	21.89378891186184	35.593993039027765
10-11	23.002084579563387	27.84076167003012	29.256538623496937	19.90061512690956
12-13	24.222415281812655	25.277455761169282	29.37037654289242	21.129752414125647
14-15	21.527513279479436	26.700855099099698	27.2800983166759	24.491533304744962
16-17	20.589075465182958	30.213638471029913	27.9241438858713	21.27314217791583
18-19	20.449939156261493	28.73746245842731	30.11782940011206	20.69476898519914
20-21	21.51300939165314	27.67914718903668	30.18592427079093	20.621919148519247
22-23	20.917818990264532	28.255919816859272	29.953876441107436	20.872384751768763
24-25	20.832984240570834	28.3470059250449	30.345553731383042	20.47445610300123
26-27	20.808506337732247	28.50124104755729	30.27581091203093	20.414441702679532
28-29	20.06458882966999	28.158052403725858	30.71319703289258	21.064161733711572
30-31	20.940627538100053	27.77715586312101	30.974907011110613	20.30730958766832
32-33	20.418854751437877	28.505485866248513	30.49542392126478	20.58023546104883
34-35	20.361842995979824	28.580238592313535	31.023141962212268	20.034776449494377
36-37	20.834784719880684	28.179592981813624	30.412737652014094	20.572884646291595
38-39	20.386194650982134	28.017685209085425	30.74529504589242	20.850825094040026
40-41	20.687408579334694	27.86801917015072	30.811056616382277	20.63351563413231
42-43	20.907467601312163	27.69179649009018	30.720796278048716	20.679939630548937
44-45	20.573764711835086	28.220921287979223	30.445698904774854	20.75961509541084
46-47	20.985074325283787	27.796872773311733	29.81604480205317	21.40200809935131
48-49	20.878078406283922	27.814240790045957	30.043758517986518	21.263922285683602
50-51	20.631697200361476	28.159016810704866	30.106953730873638	21.102332258060017
52-53	20.689863538099743	28.40835705268277	30.281288722636003	20.620490686581483
54-55	20.701161823007112	28.52665115821893	29.77352511385603	20.998661904917924
56-57	21.07058734532103	29.055620720119734	29.061885956636115	20.81190597792312
58-59	20.401827511154348	29.669114144466214	29.720455847865818	20.208602496513624
60-61	20.869321081828144	30.187262141469905	28.78793261278027	20.155484163921674
62-63	20.377302704478314	30.420526942384953	28.835077628669755	20.367092724466982
64-65	20.339181752140448	30.25057635370362	28.934404232637124	20.475837661518803
66-67	20.517064509306238	29.983189154639277	28.9082611793562	20.591485156698287
68-69	20.69156250534847	29.60729249407214	29.088469104287952	20.61267589629144
70-71	20.752883192064957	28.899068291615706	29.387036652962312	20.961011863357022
72-73	20.868194577374886	28.656256490137988	29.58929283744484	20.886256095042285
74-75	20.846519361820324	28.722209009332367	29.419228152341425	21.012043476505884
76-77	20.76512496693512	28.760805954051023	29.388381023410375	21.085688055603484
78-79	20.850628841282127	28.829154057406864	29.30492207434571	21.01529502696529
80-81	20.820035875517043	28.783264844035617	29.326861694853633	21.069837585593707
82-83	20.975806874772697	28.705731429953445	29.47634335384157	20.842118341432286
84-85	21.215801636645747	28.663341030047874	29.242427268239474	20.878430065066908
86-87	20.78044759366439	28.845065760412037	29.342331939960896	21.032154705962675
88-89	21.009922866864354	28.785468622057575	29.27793517318684	20.92667333789123
90-91	21.07767059407583	28.782523667473026	29.29976723317601	20.840038505275142
92-93	21.069282123809018	28.795589576819715	29.23773879113597	20.897389508235303
94-95	20.92731238596994	28.971043463838097	29.17796340194626	20.923680748245697
96-97	20.911554691722447	28.970353230366275	29.279722124059372	20.83836995385191
98-99	21.119229872792445	29.50046932469076	29.270003648259618	20.11029715425718
100-101	21.696217088229584	32.139356484432156	26.761779862753404	19.402646564584856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3321.0
1	2982.5
2	3598.0
3	4924.5
4	6358.0
5	9428.5
6	12429.5
7	15311.0
8	19045.0
9	21169.5
10	19829.5
11	17667.5
12	18276.0
13	22629.0
14	31899.5
15	43047.0
16	52495.0
17	59377.0
18	60723.5
19	56639.0
20	50755.0
21	45264.5
22	43541.0
23	48172.5
24	58593.5
25	74528.0
26	97259.0
27	123850.5
28	149821.5
29	176500.0
30	202114.0
31	226617.5
32	256839.5
33	291578.0
34	325476.0
35	356530.5
36	386068.5
37	411604.0
38	427265.0
39	437294.0
40	449343.5
41	455659.0
42	455183.0
43	465253.5
44	512576.0
45	541507.0
46	516889.0
47	486458.5
48	444971.5
49	403991.5
50	377119.5
51	348054.5
52	315702.0
53	283637.5
54	251985.5
55	220022.0
56	190650.5
57	163425.5
58	138229.5
59	117114.5
60	95379.5
61	72257.5
62	51520.5
63	36919.5
64	27566.5
65	19942.5
66	13873.0
67	9915.0
68	7116.5
69	4865.0
70	3338.5
71	2361.5
72	1702.0
73	1210.0
74	836.5
75	552.5
76	361.5
77	215.0
78	149.5
79	110.0
80	80.0
81	61.5
82	47.5
83	35.5
84	24.0
85	16.0
86	11.5
87	9.0
88	6.5
89	4.0
90	5.0
91	5.5
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.04688467298472275
6	0.0
7	0.0023007324062606217
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.8026736979598086E-5
28-29	4.83796939007091E-6
30-31	1.4531971694044257E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	3.0177856213379174E-5
46-47	5.07688072792722E-6
48-49	0.0
50-51	0.0
52-53	8.171242171936987E-4
54-55	1.6251079386208267E-4
56-57	0.003048002768452844
58-59	0.007705188312778887
60-61	0.003278244318520452
62-63	0.005652602445689042
64-65	0.006521079238066371
66-67	0.0050741293741238674
68-69	0.0038998474289905116
70-71	0.005445029609695498
72-73	0.0033749018341266853
74-75	0.0028573872782497595
76-77	0.0024137145385349818
78-79	6.504567329781682E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.9184336190676855E-5
88-89	6.210797060777556E-6
90-91	2.3227661910457613E-4
92-93	0.0
94-95	8.982841425451175E-5
96-97	1.2992140664347915E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	173.0
20-21	589.0
22-23	1181.0
24-25	1848.0
26-27	3938.0
28-29	9199.0
30-31	22993.0
32-33	29241.0
34-35	38815.0
36-37	58784.0
38-39	70711.0
40-41	67548.0
42-43	76591.0
44-45	90736.0
46-47	91490.0
48-49	83348.0
50-51	73653.0
52-53	68249.0
54-55	71591.0
56-57	74824.0
58-59	79947.0
60-61	89460.0
62-63	85202.0
64-65	105589.0
66-67	114342.0
68-69	103155.0
70-71	131066.0
72-73	73037.0
74-75	73373.0
76-77	78109.0
78-79	81603.0
80-81	83929.0
82-83	78719.0
84-85	78802.0
86-87	81236.0
88-89	85060.0
90-91	87558.0
92-93	84238.0
94-95	90601.0
96-97	143279.0
98-99	812787.0
100-101	6767938.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.81282004251827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40988973392288	74.93694998290678
2	7.160965794237553	12.003614748861217
3	1.882645641593555	4.7336952108813595
4	0.7216472991400626	2.4193318086798166
5	0.31609722632197423	1.3246499972831398
6	0.1754489693355787	0.8822923736140896
7	0.10292918038240355	0.6038749410760001
8	0.061117953766187845	0.4097974448297962
9	0.03992964959085789	0.30119548819674463
>10	0.12380086795606604	1.5928254221511697
>50	0.003399247324870985	0.19469757310874994
>100	0.001809698043371383	0.29673904624910685
>500	1.7929065943838478E-4	0.12028156739534673
>1k	1.3944772520713567E-4	0.1800543947665611
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.66694288344799E-6	0.0
3	0.0	9.66694288344799E-6	0.0	9.66694288344799E-6	0.0
4	0.0	9.66694288344799E-6	0.0	9.66694288344799E-6	0.0
5	0.0	9.66694288344799E-6	0.0	2.900082865034397E-5	0.0
6	0.0	9.66694288344799E-6	0.0	2.900082865034397E-5	0.0
7	0.0	9.66694288344799E-6	0.0	2.900082865034397E-5	0.0
8	0.0	9.66694288344799E-6	0.0	2.900082865034397E-5	9.66694288344799E-6
9	0.0	9.66694288344799E-6	0.0	9.66694288344799E-5	9.66694288344799E-6
10-11	9.66694288344799E-6	9.66694288344799E-6	0.0	1.0633637171792789E-4	9.66694288344799E-6
12-13	9.66694288344799E-6	9.66694288344799E-6	0.0	1.2567025748482386E-4	9.66694288344799E-6
14-15	9.66694288344799E-6	2.4167357208619977E-5	0.0	1.3533720036827186E-4	2.4167357208619977E-5
16-17	9.66694288344799E-6	3.866777153379196E-5	0.0	1.3533720036827186E-4	3.866777153379196E-5
18-19	1.933388576689598E-5	3.866777153379196E-5	0.0	1.740049719020638E-4	3.866777153379196E-5
20-21	1.933388576689598E-5	3.866777153379196E-5	0.0	2.1267274343585578E-4	3.866777153379196E-5
22-23	1.933388576689598E-5	3.866777153379196E-5	0.0	2.3684010064447576E-4	5.3168185858963944E-5
24-25	1.933388576689598E-5	3.866777153379196E-5	0.0	2.610074578530957E-4	6.766860018413593E-5
26-27	1.933388576689598E-5	4.350124297551596E-5	0.0	3.093421722703357E-4	6.766860018413593E-5
28-29	1.933388576689598E-5	8.216901450930792E-5	0.0	7.395211305837712E-4	6.766860018413593E-5
30-31	1.933388576689598E-5	1.2083678604309987E-4	0.0	0.0017062154189285703	7.250207162585992E-5
32-33	1.933388576689598E-5	1.2567025748482386E-4	0.0	0.003707272595802304	7.733554306758392E-5
34-35	1.933388576689598E-5	1.4500414325171985E-4	0.0	0.006414016603167741	8.70024859510319E-5
36-37	1.933388576689598E-5	1.4500414325171985E-4	0.0	0.010459632199890725	8.70024859510319E-5
38-39	1.933388576689598E-5	1.4500414325171985E-4	0.0	0.016617474816647094	8.70024859510319E-5
40-41	1.933388576689598E-5	1.5467108613516784E-4	0.0	0.02339883524938586	8.70024859510319E-5
42-43	1.933388576689598E-5	1.6433802901861584E-4	0.0	0.029334338179822922	8.70024859510319E-5
44-45	1.933388576689598E-5	1.6433802901861584E-4	0.0	0.036197867627071	8.70024859510319E-5
46-47	1.933388576689598E-5	1.788384433437878E-4	0.0	0.04367524794741802	1.0150290027620389E-4
48-49	1.933388576689598E-5	2.0300580055240778E-4	0.0	0.051070459253255734	1.1600331460137588E-4
50-51	1.933388576689598E-5	2.1750621487757977E-4	0.0	0.057909821343295184	1.1600331460137588E-4
52-53	1.933388576689598E-5	2.2233968631930377E-4	0.0	0.06480718509063532	1.4017067180999585E-4
54-55	1.933388576689598E-5	2.2233968631930377E-4	0.0	0.07159337899481581	1.6433802901861584E-4
56-57	1.933388576689598E-5	2.2233968631930377E-4	0.0	0.07849074274215595	1.691715004603398E-4
58-59	1.933388576689598E-5	2.561739864113717E-4	0.0	0.08441657872970956	1.740049719020638E-4
60-61	1.933388576689598E-5	2.900082865034397E-4	0.0	0.09031824736005456	1.740049719020638E-4
62-63	1.933388576689598E-5	2.900082865034397E-4	0.0	0.09604591101849749	1.836719147855118E-4
64-65	1.933388576689598E-5	2.900082865034397E-4	0.0	0.101483566390437	1.836719147855118E-4
66-67	1.933388576689598E-5	2.900082865034397E-4	0.0	0.10728373212050579	1.981723291106838E-4
68-69	1.933388576689598E-5	2.900082865034397E-4	0.0	0.112866391635697	2.1267274343585578E-4
70-71	1.933388576689598E-5	2.900082865034397E-4	0.0	0.11807687384987547	2.2233968631930377E-4
72-73	1.933388576689598E-5	2.900082865034397E-4	0.0	0.12168264354540156	2.706744007365437E-4
74-75	1.933388576689598E-5	2.9484175794516367E-4	0.0	0.12473739749657113	2.706744007365437E-4
76-77	1.933388576689598E-5	3.045087008286117E-4	0.0	0.12727497000347623	2.706744007365437E-4
78-79	1.933388576689598E-5	3.093421722703357E-4	0.0	0.12978354168173098	2.706744007365437E-4
80-81	1.933388576689598E-5	3.093421722703357E-4	0.0	0.13095807524206993	2.755078721782677E-4
82-83	1.933388576689598E-5	3.238425865955077E-4	0.0	0.13147042321489266	2.803413436199917E-4
84-85	1.933388576689598E-5	3.5284341524585163E-4	0.0	0.1320021050734823	2.803413436199917E-4
86-87	1.933388576689598E-5	4.2051201542998753E-4	0.0	0.13224861211701022	2.803413436199917E-4
88-89	1.933388576689598E-5	4.2534548687171155E-4	0.0	0.13238878278882021	2.803413436199917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17720	0.0	50.299538	1
CTTATAC	13610	0.0	46.355392	1
GTATCAA	32095	0.0	37.579494	1
TATACAC	19555	0.0	36.687035	3
TTATACA	20250	0.0	33.883877	2
ATCAACG	37030	0.0	32.351498	3
TCAACGC	37735	0.0	31.837883	4
TATCAAC	37865	0.0	31.491035	2
CAACGCA	38215	0.0	31.471603	5
AACGCAG	39955	0.0	30.160334	6
GTGGTAT	8260	0.0	27.32664	1
TGGTATC	7770	0.0	27.175749	2
ACGCAGA	45555	0.0	26.3776	7
CGCAGAG	45905	0.0	26.203953	8
ACACATC	28090	0.0	24.627396	6
TACACAT	29930	0.0	24.370451	5
ACATCTC	28705	0.0	23.950161	8
CACATCT	30250	0.0	22.89718	7
GCAGAGT	53050	0.0	22.70697	9
ATACACA	35805	0.0	21.866947	4
>>END_MODULE