FastQCFastQC Report
Fri 27 May 2016
SRR2139841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14443352
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155750.046.3242451
GTATCAA252400.029.0110151
TCAACGC324300.023.8650933
ATCAACG326350.023.6900392
CAACGCA333700.023.2175884
AACGCAG346200.022.4627935
TATCAAC347900.022.1513791
ACGCAGA411750.018.93696
CGCAGAG418250.018.6131737
GTACATG334200.017.350191
TACATGG339350.016.9043272
AGAGTAC443850.016.25555210-11
CAGAGTA488500.016.1393629
ACATGGG344750.016.0883773
GCAGAGT493350.015.9137318
GAGTACT302850.015.64185912-13
AGTACTT338800.014.3721912-13
TGGTATC73700.014.34551052
GTGGTAT84550.014.165371
CATGGGG241150.013.9062264
Produced by FastQC (version 0.11.5)