##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14443352
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259868069406604	31.0	31.0	34.0	30.0	34.0
2	31.535162197805608	31.0	31.0	34.0	30.0	34.0
3	31.529279699061547	31.0	31.0	34.0	30.0	34.0
4	35.33916067405959	37.0	35.0	37.0	33.0	37.0
5	35.124412255548435	37.0	35.0	37.0	32.0	37.0
6	35.12985752891711	37.0	35.0	37.0	32.0	37.0
7	34.9962966352963	36.0	35.0	37.0	32.0	37.0
8	34.975188515796056	36.0	35.0	37.0	32.0	37.0
9	36.455768716292454	38.0	35.0	39.0	32.0	39.0
10-11	36.332993961512535	38.0	35.0	39.0	32.0	39.0
12-13	36.26763887634948	38.0	35.0	39.0	32.0	39.0
14-15	37.236235155108034	39.0	36.0	40.0	32.0	41.0
16-17	37.262834555302675	39.0	36.0	40.0	32.0	41.0
18-19	37.28599403379493	39.0	36.0	40.0	32.0	41.0
20-21	37.26221142999015	39.0	36.0	40.0	31.0	41.0
22-23	37.18599611917749	39.0	36.0	40.0	31.0	41.0
24-25	37.07911040925693	39.0	36.0	40.0	31.0	41.0
26-27	36.877918263770546	39.0	36.0	40.0	31.0	41.0
28-29	36.71481024533831	38.5	35.5	40.0	30.0	41.0
30-31	36.55332015153208	38.0	35.0	40.0	30.0	41.0
32-33	36.78786910280138	38.0	35.0	40.0	30.0	41.0
34-35	36.78158925506446	38.5	35.0	40.0	30.0	41.0
36-37	36.648782164632635	38.0	35.0	40.0	30.0	41.0
38-39	36.51492133654128	38.0	35.0	40.0	30.0	41.0
40-41	36.47861473292191	38.0	35.0	40.0	30.0	41.0
42-43	36.394746025791	38.0	35.0	40.0	30.0	41.0
44-45	36.370413619195375	38.0	35.0	40.0	30.0	41.0
46-47	36.390283736409756	38.0	35.0	40.0	30.0	41.0
48-49	36.31163244065279	38.0	35.0	40.0	30.0	41.0
50-51	36.22246264825207	38.0	35.0	40.0	29.5	41.0
52-53	36.0900626574298	38.0	34.0	40.0	29.0	41.0
54-55	35.970623878566414	38.0	34.0	40.0	28.5	41.0
56-57	35.78290700424592	38.0	34.0	40.0	28.0	41.0
58-59	35.63117991626011	38.0	34.0	40.0	28.0	41.0
60-61	35.4714170539324	37.5	34.0	40.0	28.0	41.0
62-63	35.25238031428038	37.0	33.0	40.0	28.0	41.0
64-65	35.04236157925622	37.0	33.0	39.0	28.0	41.0
66-67	34.79759424117579	36.0	33.0	39.0	28.0	40.0
68-69	34.536625315491534	36.0	32.5	39.0	27.0	40.0
70-71	34.264394306584855	35.5	32.0	38.5	26.5	40.0
72-73	33.962141389721836	35.0	32.0	38.0	26.0	40.0
74-75	33.644400144017126	35.0	32.0	37.5	26.0	39.5
76-77	32.68396592272449	34.0	30.5	36.0	26.0	39.0
78-79	33.06466432740706	34.0	31.0	36.0	26.0	39.0
80-81	33.01018379380335	34.0	31.0	36.0	26.0	38.5
82-83	32.82334158759318	34.0	31.0	36.0	26.0	37.0
84-85	32.5777938856459	34.0	31.0	35.0	26.0	37.0
86-87	32.410306848632075	34.0	31.0	35.0	26.0	36.5
88-89	32.21470887847117	34.0	31.0	35.0	26.0	36.0
90-91	32.12227517422395	34.0	31.0	35.0	26.0	36.0
92-93	32.09283865953042	34.0	31.0	35.0	26.0	35.5
94-95	32.09453133639508	34.0	31.0	35.0	26.0	35.0
96-97	32.009855553041106	34.0	31.0	35.0	26.0	35.0
98-99	32.19529560862129	34.0	31.0	35.0	27.0	35.0
100-101	31.76646235078054	33.5	30.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	1.0
17	1.0
18	8.0
19	38.0
20	248.0
21	1586.0
22	6772.0
23	20769.0
24	50554.0
25	100868.0
26	170111.0
27	253220.0
28	344277.0
29	438524.0
30	537410.0
31	644829.0
32	774657.0
33	945170.0
34	1182481.0
35	1519701.0
36	2049266.0
37	2590408.0
38	2119294.0
39	686743.0
40	6414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.958342910980775	25.06614115615267	16.7693690495115	24.206146883355053
2	14.661673006366424	25.0477788271822	38.49931797944113	21.79123018701024
3	17.269315322371153	28.135677922964142	32.136612055151744	22.458394699512965
4	12.922166544165096	20.803668012799246	37.92207653735781	28.352088905677853
5	12.40514667232371	38.72620427723426	36.40182002072649	12.466829029715539
6	29.302422318586434	40.16972652885563	18.051121374041152	12.476729778516788
7	24.846254525957686	33.210040162422125	24.49697272489101	17.446732586729176
8	23.48274832601186	38.37135590131709	21.461396218827876	16.684499553843178
9	24.918536915807355	15.937754615410604	21.656579442223663	37.48712902655838
10-11	23.57520954969456	27.546306425267485	29.83435908783501	19.044124937202945
12-13	24.054993762479025	25.160528407826554	29.872674673089232	20.91180315660519
14-15	21.226149580789833	26.682334543947974	27.192320037620078	24.89919583764212
16-17	21.007692674110555	29.551682324158545	28.075657229706792	21.364967772024112
18-19	21.019791713004494	28.813378251530946	29.632903993324238	20.533926042140322
20-21	21.68470472236835	28.073951787386882	29.468458545534297	20.772884944710473
22-23	21.406452321069292	28.06538921736854	29.315821831159166	21.212336630402998
24-25	21.2680048436749	28.173344984513605	29.3970722028084	21.161577969003098
26-27	21.325305509563467	28.269238839863327	29.292436700869256	21.113018949703953
28-29	21.300128744363793	28.34832091384364	29.296260587913174	21.055289753879393
30-31	21.4632569826552	28.27208015668458	29.20390869806636	21.060754162593863
32-33	21.360250897425985	28.36391376899491	29.136406556919177	21.139428776659933
34-35	21.45577027804969	28.38026183927483	29.152100215252712	21.011867667422766
36-37	21.41855373110758	28.346704781687254	29.106395393601375	21.128346093603792
38-39	21.33799670680463	28.370538914200527	29.174246098743446	21.1172182802514
40-41	21.51154092771995	28.28725925203326	29.031753633070945	21.16944618717585
42-43	21.435605784103142	28.433718501462522	28.808401727092093	21.322273987342243
44-45	21.555894898209722	28.48221130563991	28.58328844221149	21.378605353938877
46-47	21.60637516461695	28.456524702955516	28.403384874929653	21.53371525749788
48-49	21.524980103895405	28.576309493212094	28.41171157858659	21.48699882430591
50-51	21.580625683768748	28.618410383449692	28.27598653087351	21.524977401908043
52-53	21.629300011542547	28.569313996615676	28.188023709131983	21.613362282709794
54-55	21.607443385869757	28.48367988894056	28.245937252653995	21.662939472535687
56-57	21.64883596211501	28.45668189704002	28.214321680921454	21.680160459923513
58-59	21.630295099859865	28.49807237806613	28.202435700223965	21.66919682185004
60-61	21.688216845561094	28.448256266075266	28.24614884434396	21.61737804401968
62-63	21.63468834870467	28.543217870896765	28.189789515041447	21.632304265357117
64-65	21.54635107299012	28.540090210623347	28.17953445758505	21.734024258801483
66-67	21.638832220942422	28.60651823444913	28.002705004426552	21.751944540181896
68-69	21.64565481104332	28.62253128233508	27.94934484932764	21.78246905729396
70-71	21.646857495574324	28.42577194752604	28.036513675026125	21.89085688187351
72-73	21.67657308692006	28.44508714278607	27.974506044516033	21.903833725777833
74-75	21.67223399998665	28.432308656920814	27.948401568233656	21.94705577485888
76-77	21.81236484439708	28.41735895018418	27.888783865457228	21.881492339961515
78-79	21.780541862293727	28.409199160922377	27.85130334159971	21.958955635184186
80-81	21.81224464388241	28.342893070362408	27.859353863780417	21.985508421974767
82-83	21.786878234487876	28.409241156159993	27.93721374320609	21.866666866146037
84-85	21.87276102425298	28.40468926771814	27.902577867020867	21.819971841008005
86-87	21.827954886620457	28.431272317463403	27.88197722554278	21.85879557037336
88-89	21.83006553436087	28.360054374621	27.968462561257912	21.841417529760214
90-91	21.84934670618718	28.332542203592464	28.085343925098886	21.73276716512147
92-93	21.919416377325295	28.261347409092224	28.05373751578102	21.765498697801466
94-95	21.73901755461501	28.314550007636484	28.177125371493915	21.769307066254594
96-97	21.57346041768359	28.418269475078468	28.265045533749067	21.74322457348887
98-99	21.69784772611331	29.012321183290595	28.385006776557166	20.904824314038933
100-101	25.396482764154854	36.14954706862377	29.202071089527614	9.25189907769377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4493.0
1	4033.5
2	4925.0
3	6490.0
4	8040.0
5	11242.0
6	14024.5
7	16955.0
8	19260.5
9	19721.5
10	18960.5
11	18052.0
12	20289.0
13	27445.5
14	44073.5
15	66830.0
16	83308.0
17	89084.5
18	89734.5
19	88913.0
20	86880.5
21	85572.5
22	87333.5
23	96395.0
24	113889.5
25	139171.5
26	171469.0
27	205756.5
28	241789.5
29	279422.5
30	313283.0
31	347227.0
32	382755.5
33	415057.5
34	445658.0
35	475609.0
36	505721.5
37	531880.5
38	550841.0
39	567159.5
40	584560.5
41	599522.0
42	607049.5
43	612869.0
44	619263.5
45	621579.0
46	617030.0
47	608973.5
48	597042.0
49	579055.0
50	558911.0
51	532717.0
52	499549.0
53	465430.0
54	429628.0
55	390724.0
56	353104.5
57	314637.5
58	273636.0
59	233066.5
60	193817.5
61	157647.0
62	125232.5
63	96665.5
64	72113.5
65	53430.0
66	39910.5
67	30295.0
68	22935.5
69	16621.0
70	12120.5
71	8915.0
72	6435.0
73	4529.0
74	3137.0
75	2193.0
76	1530.5
77	1032.0
78	714.0
79	475.0
80	333.5
81	258.5
82	200.0
83	145.5
84	108.5
85	78.5
86	60.0
87	44.5
88	27.0
89	18.0
90	12.0
91	9.5
92	7.5
93	4.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016547405339148418
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.892904638756986E-4
14-15	0.0
16-17	0.0
18-19	4.119542333386322E-4
20-21	0.0
22-23	0.0014785261220593604
24-25	2.1128081617294384E-4
26-27	0.005146013007734752
28-29	3.953027217840719E-4
30-31	0.0
32-33	3.4808574075813214E-6
34-35	2.1659021672471224E-4
36-37	0.016723806925650845
38-39	0.006440532872925422
40-41	0.012696406443176904
42-43	0.0015806137436951472
44-45	0.0027646869367562625
46-47	0.0
48-49	6.382743910435556E-4
50-51	1.4998523957761007E-5
52-53	3.785683664941556E-6
54-55	0.0041467463596153225
56-57	0.007860444243725222
58-59	0.011125818790726629
60-61	0.00580307639738218
62-63	0.006259826978382317
64-65	7.735449882020213E-4
66-67	9.034194137358269E-5
68-69	0.0
70-71	9.308746057746045E-5
72-73	4.296367004949716E-6
74-75	2.617765069982677E-5
76-77	8.866477193004544E-6
78-79	9.0167158641226E-6
80-81	0.0018729379607203595
82-83	0.0020477384878994083
84-85	0.008535617470281007
86-87	0.010057631733870698
88-89	0.04549133339332807
90-91	3.8505045655584625E-4
92-93	4.2080803246684627E-4
94-95	1.6026194066312332E-5
96-97	0.008064641561553723
98-99	5.852956638604861E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	591.0
20-21	1974.0
22-23	3609.0
24-25	6469.0
26-27	8682.0
28-29	14002.0
30-31	33164.0
32-33	46779.0
34-35	68226.0
36-37	96298.0
38-39	100838.0
40-41	113457.0
42-43	130850.0
44-45	154524.0
46-47	153948.0
48-49	141943.0
50-51	131209.0
52-53	120900.0
54-55	135606.0
56-57	144084.0
58-59	150014.0
60-61	149440.0
62-63	150677.0
64-65	166294.0
66-67	176614.0
68-69	180961.0
70-71	180457.0
72-73	176778.0
74-75	181739.0
76-77	183467.0
78-79	198107.0
80-81	198784.0
82-83	191731.0
84-85	194739.0
86-87	206398.0
88-89	222518.0
90-91	241462.0
92-93	254420.0
94-95	311702.0
96-97	392806.0
98-99	1158171.0
100-101	7568920.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.854948542525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22368278625548	77.46149642931063
2	6.858686502283838	11.777043534457793
3	1.6428939000119853	4.231517137390715
4	0.6108226613996929	2.0976859265231527
5	0.26750625705827613	1.148336796727088
6	0.1295675158402183	0.6674407443146841
7	0.07930181821215886	0.4765917465353499
8	0.050785061705880576	0.34881190875898666
9	0.03178753937694005	0.24562058017506078
>10	0.10278811702307093	1.3650958171536376
>50	0.0017531891583390249	0.10104902651994646
>100	3.988066346460969E-4	0.06413755734313785
>500	2.584503946356844E-5	0.015172794789791388
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.923600560313146E-6
2	0.0	4.154160336187888E-5	0.0	0.0	1.3847201120626292E-5
3	0.0	4.154160336187888E-5	0.0	0.0	1.3847201120626292E-5
4	0.0	4.8465203922192023E-5	0.0	0.0	1.3847201120626292E-5
5	0.0	4.8465203922192023E-5	0.0	6.923600560313146E-6	1.3847201120626292E-5
6	4.154160336187888E-5	4.8465203922192023E-5	0.0	6.923600560313146E-6	2.7694402241252584E-5
7	4.154160336187888E-5	4.8465203922192023E-5	0.0	6.923600560313146E-6	2.7694402241252584E-5
8	4.154160336187888E-5	4.8465203922192023E-5	0.0	6.923600560313146E-6	2.7694402241252584E-5
9	4.154160336187888E-5	6.923600560313146E-5	0.0	1.3847201120626292E-5	4.154160336187888E-5
10-11	4.8465203922192023E-5	6.923600560313146E-5	0.0	2.077080168093944E-5	4.8465203922192023E-5
12-13	4.8465203922192023E-5	7.269780588328804E-5	0.0	2.4232601961096012E-5	5.538880448250517E-5
14-15	4.8465203922192023E-5	1.3501021092610636E-4	0.0	2.7694402241252584E-5	6.577420532297489E-5
16-17	9.00068072840709E-5	1.5231921232688921E-4	0.0	4.154160336187888E-5	6.923600560313146E-5
18-19	9.693040784438405E-5	1.557810126070458E-4	0.0	9.00068072840709E-5	7.615960616344461E-5
20-21	9.693040784438405E-5	1.5924281288720235E-4	0.0	1.384720112062629E-4	7.962140644360118E-5
22-23	1.038540084046972E-4	1.800136145681418E-4	0.0	2.769440224125258E-4	1.038540084046972E-4
24-25	1.0731580868485377E-4	1.9732261596892468E-4	0.0	7.096690574320975E-4	1.1770120952532349E-4
26-27	1.1077760896501034E-4	2.1463161736970753E-4	0.0	0.0022432465815414595	1.1770120952532349E-4
28-29	1.1077760896501034E-4	2.1463161736970753E-4	0.0	0.011763197351972034	1.1770120952532349E-4
30-31	1.1077760896501034E-4	2.5271142045142983E-4	0.0	0.021058131104192433	1.1770120952532349E-4
32-33	1.1423940924516692E-4	2.561732207315864E-4	0.0	0.034566075797363385	1.2462481008563664E-4
34-35	1.280866103657932E-4	3.1502382549424815E-4	0.0	0.05376522015111174	1.280866103657932E-4
36-37	1.3154841064594977E-4	3.80798030817223E-4	0.0	0.08078803313801394	1.3501021092610636E-4
38-39	1.3154841064594977E-4	3.9464523193784935E-4	0.0	0.12559411416408048	1.384720112062629E-4
40-41	1.3154841064594977E-4	4.3618683529972823E-4	0.0	0.20152524150903472	1.4539561176657608E-4
42-43	1.3154841064594977E-4	4.4311043586004134E-4	0.0	0.2410278445059014	1.4885741204673263E-4
44-45	1.3154841064594977E-4	4.984992403425465E-4	0.0	0.29859412136462504	1.557810126070458E-4
46-47	1.3154841064594977E-4	5.469644442647385E-4	0.0	0.3606503531867118	1.6962821372767207E-4
48-49	1.384720112062629E-4	5.538880448250516E-4	0.0	0.41264313159438337	1.7309001400782865E-4
50-51	1.6962821372767207E-4	5.781206467861478E-4	0.0	0.45930127577033364	1.7309001400782865E-4
52-53	1.7309001400782865E-4	5.815824470663043E-4	0.0	0.5188546259898672	1.8347541484829837E-4
54-55	1.7655181428798524E-4	5.815824470663043E-4	0.0	0.634596456556622	1.9732261596892468E-4
56-57	1.800136145681418E-4	5.885060476266174E-4	0.0	0.7569987908623981	2.0078441624908123E-4
58-59	1.800136145681418E-4	5.954296481869305E-4	0.0	0.8577302554143942	2.0078441624908123E-4
60-61	1.9732261596892468E-4	6.231240504281832E-4	0.0	0.9245395390211357	2.0078441624908123E-4
62-63	2.2155521793002067E-4	6.369712515488094E-4	0.0	0.9812922928140226	2.077080168093944E-4
64-65	2.2847881849033384E-4	7.062072571519409E-4	0.0	1.0060337794163017	2.077080168093944E-4
66-67	2.7348222213236927E-4	7.096690574320975E-4	0.0	1.0171738527178456	2.1116981708955095E-4
68-69	2.769440224125258E-4	7.200544582725672E-4	0.0	1.027552329957755	2.1463161736970753E-4
70-71	2.942530238133087E-4	7.339016593931935E-4	0.0	1.0376227069727304	2.1463161736970753E-4
72-73	3.184856257744047E-4	7.408252599535066E-4	0.0	1.045242129389355	2.3540241905064697E-4
74-75	3.184856257744047E-4	7.61596061634446E-4	0.0	1.051940712931458	2.3540241905064697E-4
76-77	3.288710266148744E-4	8.031376649963249E-4	0.0	1.0579850162206115	2.3886421933080356E-4
78-79	3.3925642745534414E-4	8.169848661169513E-4	0.0	1.0632711852484105	2.423260196109601E-4
80-81	3.3925642745534414E-4	8.204466663971078E-4	0.0	1.0663487256974697	2.492496201712733E-4
82-83	3.3925642745534414E-4	8.27370266957421E-4	0.0	1.068169632644832	2.700204218522127E-4
84-85	3.427182277355007E-4	8.377556677978907E-4	0.0	1.0698797619832292	2.700204218522127E-4
86-87	3.496418282958139E-4	8.654500700391432E-4	0.0	1.0701428588045214	2.700204218522127E-4
88-89	3.5310362857597047E-4	8.723736705994565E-4	0.0	1.0701878622081633	2.769440224125258E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15575	0.0	46.324245	1
GTATCAA	25240	0.0	29.011015	1
TCAACGC	32430	0.0	23.865093	3
ATCAACG	32635	0.0	23.690039	2
CAACGCA	33370	0.0	23.217588	4
AACGCAG	34620	0.0	22.462793	5
TATCAAC	34790	0.0	22.151379	1
ACGCAGA	41175	0.0	18.9369	6
CGCAGAG	41825	0.0	18.613173	7
GTACATG	33420	0.0	17.35019	1
TACATGG	33935	0.0	16.904327	2
AGAGTAC	44385	0.0	16.255552	10-11
CAGAGTA	48850	0.0	16.139362	9
ACATGGG	34475	0.0	16.088377	3
GCAGAGT	49335	0.0	15.913731	8
GAGTACT	30285	0.0	15.641859	12-13
AGTACTT	33880	0.0	14.37219	12-13
TGGTATC	7370	0.0	14.3455105	2
GTGGTAT	8455	0.0	14.16537	1
CATGGGG	24115	0.0	13.906226	4
>>END_MODULE