FastQCFastQC Report
Fri 27 May 2016
SRR2139840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11872294
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113250.043.0117151
GTATCAA191350.031.8151131
TCAACGC235900.025.0422364
ATCAACG239000.024.7517053
CAACGCA242250.024.4365485
AACGCAG249000.023.8070166
TATCAAC257250.023.2987212
GTACATG277650.020.3031711
ACGCAGA296300.019.9930827
TACATGG277050.019.9035262
CGCAGAG298200.019.8244828
ACATGGG280600.019.0819933
AGAGTAC322050.016.74714310-11
CATGGGG210350.016.4959264
GCAGAGT360950.016.0943099
GAGTACT216550.015.55107512-13
CAGAGTA353800.014.90845110-11
ATGGGGG126700.014.5502915
AGTACTT246650.014.30938412-13
GTACTTT249700.013.54454814-15
Produced by FastQC (version 0.11.5)