##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139840_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11872294
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.541336914331804	31.0	31.0	34.0	30.0	34.0
2	31.78961049987475	31.0	31.0	34.0	30.0	34.0
3	31.803288058735742	31.0	31.0	34.0	30.0	34.0
4	35.57199964893053	37.0	35.0	37.0	33.0	37.0
5	35.392396953781635	37.0	35.0	37.0	33.0	37.0
6	35.37996995357426	37.0	35.0	37.0	33.0	37.0
7	35.243829793972424	37.0	35.0	37.0	32.0	37.0
8	35.222643492487634	37.0	35.0	37.0	32.0	37.0
9	36.73142646231638	39.0	35.0	39.0	32.0	39.0
10-11	36.64624132454941	38.5	35.0	39.0	32.0	39.0
12-13	36.57556799890568	38.0	35.0	39.0	32.0	39.0
14-15	37.61892015140461	39.0	36.0	41.0	32.0	41.0
16-17	37.65799010705092	39.0	36.0	41.0	32.0	41.0
18-19	37.68113719218881	39.0	36.5	41.0	32.0	41.0
20-21	37.65509879876525	39.0	37.0	41.0	32.0	41.0
22-23	37.58824963391166	39.0	36.0	41.0	32.0	41.0
24-25	37.48907520662584	39.0	36.0	41.0	32.0	41.0
26-27	37.3137727630499	39.0	36.0	40.5	31.5	41.0
28-29	37.16507305186757	39.0	36.0	40.0	31.0	41.0
30-31	37.01816829051346	39.0	36.0	40.0	31.0	41.0
32-33	37.25161653262185	39.0	36.0	40.0	31.0	41.0
34-35	37.256630757161886	39.0	36.0	41.0	31.0	41.0
36-37	37.142208352149666	39.0	36.0	40.5	31.0	41.0
38-39	37.02394005354269	39.0	36.0	40.0	31.0	41.0
40-41	36.99786194772628	39.0	35.5	40.0	31.0	41.0
42-43	36.921826032400816	39.0	35.0	40.0	30.0	41.0
44-45	36.897064820911964	39.0	35.0	40.0	30.5	41.0
46-47	36.91114038374485	39.0	35.0	40.0	30.5	41.0
48-49	36.837594360833556	39.0	35.0	40.0	30.0	41.0
50-51	36.75159546800012	39.0	35.0	40.0	30.0	41.0
52-53	36.62700382514995	39.0	35.0	40.0	30.0	41.0
54-55	36.51326597889395	38.0	35.0	40.0	30.0	41.0
56-57	36.33547410222616	38.0	35.0	40.0	29.5	41.0
58-59	36.184751100580215	38.0	34.0	40.0	29.0	41.0
60-61	36.02486910148685	38.0	34.0	40.0	29.0	41.0
62-63	35.80368785942039	38.0	34.0	40.0	28.5	41.0
64-65	35.59333617944102	37.0	34.0	40.0	28.0	41.0
66-67	35.34533507824628	37.0	34.0	39.5	28.0	41.0
68-69	35.075654774206946	36.0	33.5	39.0	28.0	40.5
70-71	34.79939561639291	36.0	33.0	39.0	28.0	40.0
72-73	34.49679123949801	35.5	33.0	38.5	28.0	40.0
74-75	34.17507829915719	35.0	32.5	38.0	28.0	40.0
76-77	33.14018540786988	34.5	31.0	36.5	26.0	39.0
78-79	33.51179730028812	35.0	32.0	37.0	27.0	39.0
80-81	33.44620390735032	35.0	32.0	36.0	27.0	39.0
82-83	33.25543456427344	35.0	32.0	36.0	27.0	37.5
84-85	33.01765822329146	34.5	32.0	35.5	27.0	37.0
86-87	32.84751576046346	34.0	32.0	35.0	27.0	37.0
88-89	32.65336571352213	34.0	32.0	35.0	27.0	36.0
90-91	32.56013511550437	34.0	32.0	35.0	27.0	36.0
92-93	32.521194300837756	34.0	32.0	35.0	27.0	36.0
94-95	32.51273702231214	34.0	32.0	35.0	27.0	35.0
96-97	32.42139176943655	34.0	31.5	35.0	27.0	35.0
98-99	32.57061031572569	34.0	32.0	35.0	28.0	35.0
100-101	32.12553179696245	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	21.0
20	150.0
21	845.0
22	3791.0
23	12368.0
24	30699.0
25	62181.0
26	108361.0
27	166140.0
28	231334.0
29	302009.0
30	376540.0
31	460218.0
32	560307.0
33	690505.0
34	873949.0
35	1131792.0
36	1558178.0
37	2168212.0
38	2157301.0
39	952668.0
40	24722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.03336524516661	23.049917732832427	15.004269604509457	24.912447417491514
2	14.554882516652807	23.72449918602752	39.19207366764704	22.528544629672634
3	17.147587483935286	27.533389924474577	31.704791003322523	23.614231588267607
4	12.3508818093622	19.42624567754134	38.707801541976636	29.515070971119815
5	12.517176545661691	38.4107907031278	36.37219563464315	12.69983711656736
6	29.574798265608987	39.3985357842385	18.381131734102947	12.645534216049569
7	25.35216867102516	32.934671260667905	24.381101074484846	17.332058993822088
8	23.874678305641687	37.538415069572906	21.67715860136213	16.909748023423273
9	25.207537818723154	15.936271456889461	21.54894412149834	37.307246602889045
10-11	23.695677516072294	27.149757241523837	29.866970107040814	19.287595135363055
12-13	24.251335190138327	24.801684576104375	29.799799708318236	21.14718052543906
14-15	21.389568856701157	26.285695081338112	27.0643735743067	25.26036248765403
16-17	21.24409149571262	29.194825363994525	27.900298796508917	21.66078434378394
18-19	21.27580577424019	28.56784217577702	29.335409660480416	20.820942389502374
20-21	21.952795518255417	27.879240983565268	29.088957225492347	21.07900627268697
22-23	21.660449756550396	27.835584775470718	28.957880516453766	21.546084951525117
24-25	21.4988257045562	27.901744294323017	29.079932988895806	21.519497012224974
26-27	21.533446255565664	28.013834800226295	28.951509484708705	21.501209459499336
28-29	21.49029108973849	28.09037951168512	28.975421448842773	21.443907949733617
30-31	21.72005022513039	27.997921223580807	28.83292879831772	21.449099752971083
32-33	21.529482195524114	28.14154171038263	28.82762711423678	21.501348979856484
34-35	21.64621612592858	28.14385316444997	28.836518848864813	21.37341186075664
36-37	21.626735976991462	28.087723382199435	28.79226913034175	21.493271510467345
38-39	21.50361097928089	28.193692073263264	28.837607506780827	21.46508944067502
40-41	21.714202651824948	28.03541985518579	28.71004526264501	21.54033223034425
42-43	21.608890569718252	28.202478881181726	28.512638393730683	21.67599215536934
44-45	21.722870701900927	28.220304566727496	28.345782724430425	21.711042006941156
46-47	21.765830344759614	28.180904479310463	28.207658246897104	21.845606929032815
48-49	21.67065526320081	28.293406257806108	28.212544112251432	21.82339436674165
50-51	21.693886376526486	28.363739902306413	28.10410921039041	21.838264510776693
52-53	21.814579292031205	28.25716683377553	28.003953955046402	21.92429991914687
54-55	21.76605623752926	28.167853220657836	28.096320646161477	21.969769895651428
56-57	21.787204740215454	28.204635229554686	28.061458394220963	21.9467016360089
58-59	21.745968304855495	28.236196590351003	28.027194160161738	21.99064094463176
60-61	21.81495445101494	28.16198872048944	28.07493330467199	21.94812352382363
62-63	21.781424628140865	28.27384652913711	28.032749418868335	21.911979423853694
64-65	21.722818728864233	28.24728872232477	27.988074977851817	22.041817570959182
66-67	21.79208556440533	28.37560880390868	27.79021555254934	22.042090079136646
68-69	21.781894718187324	28.33805620617948	27.813190093893343	22.066858981739855
70-71	21.777928915676778	28.11434694188655	27.878461537172942	22.22926260526373
72-73	21.809752418653208	28.13734249280437	27.806364556883118	22.246540531659306
74-75	21.734833292557713	28.216400792857517	27.801381161381588	22.247384753203185
76-77	21.95145026553941	28.164061012722584	27.707982521862647	22.17650619987536
78-79	21.934876211882038	28.13452749973255	27.669980404644264	22.260615883741153
80-81	21.91706286390195	28.143200790868665	27.639992156900256	22.29974418832913
82-83	21.923516396531287	28.09596824273493	27.741828616720525	22.23868674401325
84-85	21.988369232790436	28.10917803211729	27.744620752443673	22.157831982648602
86-87	21.912907470169802	28.133994249941225	27.796257314377588	22.15684096551139
88-89	21.93173126026672	28.1490993563793	27.79768594545513	22.12148343789885
90-91	21.964095311368148	28.148394638099482	27.86632325502373	22.021186795508644
92-93	22.05256325849212	28.015481814852787	27.81903730347665	22.112917623178443
94-95	21.85087797966503	28.022103626046537	27.99228458834891	22.134733805939526
96-97	21.728181695843354	28.056270352354023	28.10388982284956	22.111658128953064
98-99	21.896353627769834	28.675283306963745	28.18801562284786	21.24034744241856
100-101	25.648337699458683	35.872833232834644	29.02276414173139	9.456064925975284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2538.0
1	2331.0
2	3162.0
3	4427.0
4	5747.0
5	8494.0
6	10998.0
7	13353.0
8	15077.0
9	15223.0
10	14395.5
11	13470.0
12	14658.5
13	19308.5
14	30234.5
15	44862.0
16	55815.5
17	60878.0
18	62437.5
19	62575.5
20	62226.5
21	63046.5
22	66400.5
23	74606.0
24	88905.0
25	109645.0
26	136153.5
27	163985.5
28	192923.0
29	223162.5
30	250947.5
31	279087.5
32	307159.0
33	332858.0
34	358905.5
35	383212.0
36	408862.5
37	431811.0
38	447836.0
39	461367.0
40	474454.5
41	486875.0
42	494740.5
43	500662.0
44	506366.5
45	509156.5
46	507948.5
47	503508.0
48	495747.0
49	483687.5
50	469599.0
51	452891.5
52	430845.5
53	405991.5
54	379894.5
55	351468.5
56	322065.5
57	291087.5
58	257908.5
59	222905.0
60	186908.0
61	152352.0
62	121202.5
63	93812.5
64	69909.5
65	52151.5
66	39196.5
67	29669.0
68	22688.5
69	16489.0
70	11735.5
71	8505.0
72	6080.5
73	4232.0
74	2917.5
75	2041.0
76	1419.0
77	931.5
78	651.0
79	475.0
80	331.5
81	225.0
82	163.0
83	111.0
84	76.0
85	56.5
86	44.0
87	33.0
88	21.0
89	13.0
90	9.0
91	7.0
92	6.0
93	3.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0022826254134205234
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.138924625687335E-4
14-15	0.0
16-17	0.0
18-19	4.043026562516056E-4
20-21	0.0
22-23	0.0014026867727985253
24-25	2.0645984193855846E-4
26-27	0.005100632098994417
28-29	3.542938943773981E-4
30-31	0.0
32-33	0.0
34-35	2.2109333289228048E-4
36-37	0.020643568707223774
38-39	0.008264094775760018
40-41	0.01367338066165863
42-43	0.001728171516022672
44-45	0.0029703861368975603
46-47	4.488133934892885E-6
48-49	6.314121264197914E-4
50-51	9.189327625168061E-6
52-53	1.3932186383117571E-5
54-55	0.004486718257981888
56-57	0.009153970399345283
58-59	0.012185035459414531
60-61	0.006301331933080635
62-63	0.0070403846230697965
64-65	8.099270255749957E-4
66-67	1.369635713930986E-4
68-69	5.151756316156279E-6
70-71	5.7584064751291544E-5
72-73	0.0
74-75	8.64691038596079E-5
76-77	1.0988253282534637E-5
78-79	0.0
80-81	0.002157716800865455
82-83	0.00214010359261336
84-85	0.010254023345623198
86-87	0.011503610363868044
88-89	0.04790989842585448
90-91	5.653834201060772E-4
92-93	6.306984895672173E-4
94-95	0.0
96-97	0.009507961620074695
98-99	1.079441652328683E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	425.0
20-21	1313.0
22-23	2436.0
24-25	5276.0
26-27	6384.0
28-29	11211.0
30-31	29636.0
32-33	42049.0
34-35	61371.0
36-37	88988.0
38-39	94474.0
40-41	102380.0
42-43	115589.0
44-45	135657.0
46-47	135893.0
48-49	126660.0
50-51	119355.0
52-53	109779.0
54-55	120888.0
56-57	127208.0
58-59	133763.0
60-61	135341.0
62-63	130905.0
64-65	141396.0
66-67	150161.0
68-69	153964.0
70-71	152647.0
72-73	148118.0
74-75	151117.0
76-77	151862.0
78-79	162836.0
80-81	162731.0
82-83	156705.0
84-85	157353.0
86-87	165120.0
88-89	176051.0
90-91	188091.0
92-93	196972.0
94-95	235034.0
96-97	293745.0
98-99	919910.0
100-101	6171500.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.5636421735174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.83986064261582	78.63429191759582
2	6.460559274588699	11.184990825725906
3	1.5307016834850802	3.975093384108095
4	0.5378408796834419	1.8622986182082912
5	0.24879090996801628	1.0768123653247574
6	0.13365834295303067	0.6941971783734849
7	0.07278271222956177	0.4410235660500512
8	0.043339688158065916	0.3001313006101318
9	0.03016121795137162	0.23497783904340022
>10	0.09973153493726868	1.3753623224206653
>50	0.002013544578573869	0.1212240353770008
>100	5.135832299534145E-4	0.06714226933068887
>500	3.065944102363659E-5	0.019107876727774607
>1k	1.532618010589396E-5	0.01334650110396927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.422972005241784E-6	0.0	0.0	0.0
3	0.0	1.684594401048357E-5	0.0	8.422972005241784E-6	0.0
4	0.0	9.265269205765963E-5	0.0	2.526891601572535E-5	0.0
5	0.0	1.0949863606814318E-4	0.0	2.526891601572535E-5	0.0
6	2.526891601572535E-5	1.1792160807338498E-4	0.0	2.526891601572535E-5	0.0
7	2.526891601572535E-5	1.1792160807338498E-4	0.0	3.369188802096714E-5	0.0
8	2.526891601572535E-5	1.2634458007862676E-4	0.0	4.211486002620892E-5	0.0
9	2.526891601572535E-5	1.6845944010483567E-4	0.0	1.0949863606814318E-4	0.0
10-11	3.790337402358803E-5	1.6845944010483567E-4	0.0	1.3476755208386855E-4	0.0
12-13	4.211486002620892E-5	1.6845944010483567E-4	0.0	1.516134960943521E-4	0.0
14-15	4.6326346028829806E-5	2.147857861336655E-4	0.0	1.8109389811269834E-4	0.0
16-17	6.738377604193427E-5	2.1899727213628637E-4	0.0	1.9372835612056102E-4	0.0
18-19	6.738377604193427E-5	2.4426618815201175E-4	0.0	2.4426618815201175E-4	0.0
20-21	6.738377604193427E-5	2.7795807617297886E-4	0.0	3.663992822280176E-4	0.0
22-23	7.159526204455517E-5	2.821695621755998E-4	0.0	7.243755924507935E-4	0.0
24-25	8.844120605503873E-5	2.948040201834624E-4	0.0	0.001398213352870136	0.0
26-27	1.010756640629014E-4	3.032269921887042E-4	0.0	0.0035713401302225165	0.0
28-29	1.010756640629014E-4	3.032269921887042E-4	0.0	0.017654549322986778	0.0
30-31	1.010756640629014E-4	3.11649964193946E-4	0.0	0.02833908931163598	0.0
32-33	1.0528715006552229E-4	3.2849590820442957E-4	0.0	0.03968483260269667	0.0
34-35	1.1371012207076408E-4	3.958796842463638E-4	0.0	0.0565055076971645	0.0
36-37	1.1792160807338498E-4	4.548404882830563E-4	0.0	0.079584450991527	0.0
38-39	1.1792160807338498E-4	4.548404882830563E-4	0.0	0.11831327627162871	0.0
40-41	1.3476755208386855E-4	4.7589791829616077E-4	0.0	0.19040970515049577	0.0
42-43	1.4319052408911032E-4	4.969553483092652E-4	0.0	0.22610626050871047	0.0
44-45	1.474020100917312E-4	5.643391243511995E-4	0.0	0.2776632721527954	0.0
46-47	1.516134960943521E-4	6.23299928387892E-4	0.0	0.3330401016012575	0.0
48-49	1.516134960943521E-4	6.527803304062383E-4	0.0	0.3777408140330757	0.0
50-51	1.600364680995939E-4	6.822607324245845E-4	0.0	0.4202094388835047	0.0
52-53	1.600364680995939E-4	6.822607324245845E-4	0.0	0.4754557122658856	0.0
54-55	1.6424795410221478E-4	6.906837044298263E-4	0.0	0.5868453055492056	0.0
56-57	1.6845944010483567E-4	6.906837044298263E-4	0.0	0.7011703045763522	0.0
58-59	1.6845944010483567E-4	6.906837044298263E-4	0.0	0.7937345554279569	0.0
60-61	1.6845944010483567E-4	7.327985644560352E-4	0.0	0.849044001100377	0.0
62-63	1.6845944010483567E-4	7.41221536461277E-4	0.0	0.8801205563137167	0.0
64-65	1.6845944010483567E-4	8.001823404979694E-4	0.0	0.9011948322708316	0.0
66-67	1.6845944010483567E-4	8.170282845084531E-4	0.0	0.9116814324173577	0.0
68-69	1.8951687011794014E-4	8.338742285189366E-4	0.0	0.921730038019611	0.0
70-71	2.147857861336655E-4	8.465086865267993E-4	0.0	0.9310205761413928	0.0
72-73	2.3584321614676995E-4	8.507201725294201E-4	0.0	0.9381590449158351	0.0
74-75	2.4426618815201175E-4	8.717776025425246E-4	0.0	0.9441772584135804	0.0
76-77	2.569006461598744E-4	9.181039485713545E-4	0.0	0.949702728049019	0.0
78-79	2.611121321624953E-4	9.265269205765962E-4	0.0	0.9547944146261876	0.0
80-81	2.611121321624953E-4	9.391613785844589E-4	0.0	0.9578435304920851	0.0
82-83	2.611121321624953E-4	9.475843505897007E-4	0.0	0.9596713154172227	0.0
84-85	2.611121321624953E-4	9.517958365923215E-4	0.0	0.9613011605002368	0.0
86-87	2.653236181651162E-4	9.517958365923215E-4	0.0	0.961600176006423	0.0
88-89	2.695351041677371E-4	0.0010023336686237722	0.0	0.9616591368104597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11325	0.0	43.011715	1
GTATCAA	19135	0.0	31.815113	1
TCAACGC	23590	0.0	25.042236	4
ATCAACG	23900	0.0	24.751705	3
CAACGCA	24225	0.0	24.436548	5
AACGCAG	24900	0.0	23.807016	6
TATCAAC	25725	0.0	23.298721	2
GTACATG	27765	0.0	20.303171	1
ACGCAGA	29630	0.0	19.993082	7
TACATGG	27705	0.0	19.903526	2
CGCAGAG	29820	0.0	19.824482	8
ACATGGG	28060	0.0	19.081993	3
AGAGTAC	32205	0.0	16.747143	10-11
CATGGGG	21035	0.0	16.495926	4
GCAGAGT	36095	0.0	16.094309	9
GAGTACT	21655	0.0	15.551075	12-13
CAGAGTA	35380	0.0	14.908451	10-11
ATGGGGG	12670	0.0	14.550291	5
AGTACTT	24665	0.0	14.309384	12-13
GTACTTT	24970	0.0	13.544548	14-15
>>END_MODULE