FastQCFastQC Report
Fri 27 May 2016
SRR2139839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8531663
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83950.045.0361821
GTATCAA145350.032.4512371
ACCGAGG86100.029.95672692-93
AGACCGA84550.029.51836690-91
GACCGAG85650.029.3327590-91
GAGACCG86800.028.71600788-89
CGAGACC86700.028.67503588-89
ACGAGAC90700.028.07964386-87
CACGAGA94500.026.4864286-87
GCCCACG93000.026.30530482-83
CCCACGA94950.025.99148284-85
TCAACGC177450.025.6591364
CCACGAG98600.025.3704984-85
ATCAACG180150.025.2291933
CAACGCA184200.024.7854275
TATCAAC189400.024.1485272
CGAGCCC100200.024.05021980-81
AACGCAG190300.024.0124196
ATCTCCG99350.023.66129974-75
TCCGAGC99250.023.64808578-79
Produced by FastQC (version 0.11.5)