##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139839_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8531663
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.589663000050518	31.0	31.0	34.0	30.0	34.0
2	31.829986135176693	31.0	31.0	34.0	30.0	34.0
3	31.843735740617042	31.0	31.0	34.0	30.0	34.0
4	35.60311160907317	37.0	35.0	37.0	33.0	37.0
5	35.42336810537407	37.0	35.0	37.0	33.0	37.0
6	35.41467331749976	37.0	35.0	37.0	33.0	37.0
7	35.27638714749985	37.0	35.0	37.0	32.0	37.0
8	35.25655584380208	37.0	35.0	37.0	32.0	37.0
9	36.77061142710395	39.0	37.0	39.0	32.0	39.0
10-11	36.693505064604636	39.0	35.0	39.0	32.0	39.0
12-13	36.624662917417155	39.0	35.0	39.0	32.0	39.0
14-15	37.680541531000465	39.0	37.0	41.0	32.0	41.0
16-17	37.71851132657255	39.0	37.0	41.0	32.0	41.0
18-19	37.744992506150325	39.5	37.0	41.0	32.0	41.0
20-21	37.72163210714518	39.0	37.0	41.0	32.0	41.0
22-23	37.65407840896258	39.0	37.0	41.0	32.0	41.0
24-25	37.5567267757703	39.0	36.0	41.0	32.0	41.0
26-27	37.38189073482304	39.0	36.0	41.0	32.0	41.0
28-29	37.23483903836123	39.0	36.0	40.0	31.0	41.0
30-31	37.088149393125704	39.0	36.0	40.0	31.0	41.0
32-33	37.31705090214933	39.0	36.0	40.5	31.0	41.0
34-35	37.32089174893849	39.0	36.0	41.0	31.0	41.0
36-37	37.211023908268274	39.0	36.0	41.0	31.0	41.0
38-39	37.098306682821715	39.0	36.0	40.0	31.0	41.0
40-41	37.07751835618022	39.0	36.0	40.0	31.0	41.0
42-43	37.004294428106476	39.0	35.0	40.0	31.0	41.0
44-45	36.97663867375839	39.0	35.0	40.0	30.5	41.0
46-47	36.99096413843101	39.0	35.0	40.0	31.0	41.0
48-49	36.9130002989702	39.0	35.0	40.0	30.0	41.0
50-51	36.829024004427005	39.0	35.0	40.0	30.0	41.0
52-53	36.70455556807856	39.0	35.0	40.0	30.0	41.0
54-55	36.592257625730035	38.5	35.0	40.0	30.0	41.0
56-57	36.41712351511784	38.0	35.0	40.0	30.0	41.0
58-59	36.26823439642011	38.0	34.5	40.0	29.0	41.0
60-61	36.11360397430202	38.0	34.0	40.0	29.0	41.0
62-63	35.88392161738824	38.0	34.0	40.0	29.0	41.0
64-65	35.680527572767105	37.0	34.0	40.0	29.0	41.0
66-67	35.43625864243754	37.0	34.0	39.5	28.5	41.0
68-69	35.165453098136105	36.0	34.0	39.0	28.0	41.0
70-71	34.89140816471259	36.0	33.0	39.0	28.0	40.0
72-73	34.58190764061456	35.5	33.0	38.5	28.0	40.0
74-75	34.2675675439105	35.0	33.0	38.0	28.0	40.0
76-77	33.21639583814172	34.5	31.5	36.5	26.0	39.0
78-79	33.58730587530536	35.0	32.0	37.0	27.0	39.0
80-81	33.50678194153183	35.0	32.0	36.0	28.0	39.0
82-83	33.30833108194106	35.0	32.0	36.0	27.5	37.5
84-85	33.082097448912634	35.0	32.0	35.5	27.0	37.0
86-87	32.92203972678464	34.0	32.0	35.0	27.0	37.0
88-89	32.73547446686344	34.0	32.0	35.0	27.0	36.0
90-91	32.63296833771261	34.0	32.0	35.0	27.0	36.0
92-93	32.60090355056573	34.0	32.0	35.0	27.0	36.0
94-95	32.579264311720976	34.0	32.0	35.0	27.0	35.0
96-97	32.48488402563532	34.0	32.0	35.0	27.0	35.0
98-99	32.637138127056204	34.0	32.0	35.0	28.0	35.0
100-101	32.195130134869046	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	17.0
20	105.0
21	509.0
22	2387.0
23	8272.0
24	20408.0
25	42343.0
26	74380.0
27	115029.0
28	161585.0
29	211186.0
30	265494.0
31	323872.0
32	395286.0
33	489909.0
34	618831.0
35	799919.0
36	1103849.0
37	1556311.0
38	1597313.0
39	723560.0
40	21097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.50307530899896	22.68143971462539	14.849859869054837	24.965625107320815
2	14.558911112228548	23.440280347201245	39.22116843137855	22.779640109191657
3	17.144734854154457	27.33033407437682	31.629366982732442	23.895564088736275
4	12.355293452167531	19.121500696874687	38.64979195732415	29.873413893633632
5	12.547928815284898	38.31554293693973	36.33241256716305	12.804115680612327
6	29.70232180994491	39.08552177928266	18.391830525889265	12.820325884883168
7	25.44611759747191	32.90002195351598	24.252247187916353	17.401613261095754
8	24.05313008729951	37.25843367230984	21.664369537334046	17.024066703056604
9	25.233568180084	15.96434364554718	21.517047731491505	37.28504044287732
10-11	23.723780463433684	27.085833090219342	29.772196815556356	19.41818963079062
12-13	24.265084578567617	24.746377992201694	29.746510441073497	21.242026988157196
14-15	21.444459304123946	26.250527007454465	27.034401147818425	25.27061254060316
16-17	21.251026909993982	29.147142825496037	27.89035385012277	21.711476414387207
18-19	21.287423916178234	28.509628263731422	29.325762209585708	20.877185610504633
20-21	21.957971324861628	27.84312401633388	29.070527168261805	21.12837749054268
22-23	21.665608438564114	27.791899209165976	28.97637415882003	21.56611819344988
24-25	21.488221460775854	27.873402873539195	29.081666246764325	21.55670941892063
26-27	21.540972885912844	27.958614488519107	28.95865367445591	21.54175895111214
28-29	21.49856014679788	28.05795661517816	28.944541587646516	21.498941650377443
30-31	21.71887140133349	27.983515970997015	28.835160238959993	21.4624523887095
32-33	21.537377136283286	28.124813403000537	28.775911305115336	21.56189815560084
34-35	21.630109278522607	28.094382879125913	28.827012471638554	21.44849537071293
36-37	21.618185251887496	28.062610102666174	28.764407550101367	21.554797095344963
38-39	21.526023635619545	28.157919918431002	28.793709037770892	21.52234740817856
40-41	21.711932095918588	28.005653261003648	28.686306903706015	21.59610773937175
42-43	21.60226788862534	28.152288004387916	28.517588784884396	21.72785532210235
44-45	21.737868561966646	28.174323028430425	28.329032347058074	21.75877606254485
46-47	21.75931025141059	28.16632752883235	28.1604757878893	21.913886431867756
48-49	21.657684912207014	28.318962631946455	28.133500796762572	21.889851659083966
50-51	21.69977401840146	28.34152978608757	28.075358272866026	21.883337922644944
52-53	21.834870067550526	28.2077023765206	27.950800704034044	22.006626851894826
54-55	21.761104428540452	28.149927833487258	28.036919904884062	22.052047833088228
56-57	21.789075721578932	28.212638163735303	28.022562968013588	21.97572314667218
58-59	21.778742811223342	28.211915370187977	27.985969173815217	22.023372644773463
60-61	21.70404589523872	28.01092586228323	28.174374462315598	22.110653780162444
62-63	21.9625931230733	28.095696632677363	28.107640466147615	21.834069778101718
64-65	21.641852537840766	28.076219330202758	28.073795370278336	22.20813276167814
66-67	21.69312562003508	28.19163400161938	27.896816266751774	22.21842411159377
68-69	21.686633232173996	28.442503062100087	27.904446537660334	21.966417168065586
70-71	21.6693739276791	28.225540174641868	27.959605685809986	22.14548021186905
72-73	21.68956782096473	28.20449754848438	27.63557300687884	22.47036162367205
74-75	21.655573949230945	28.256847206827878	27.60123784328337	22.486341000657802
76-77	21.820049204930424	27.907215310910942	27.869345916728548	22.403389567430086
78-79	21.806388073542248	27.897592635235853	27.796962914100774	22.499056377121125
80-81	22.115563485268837	27.89524627150727	27.475543471141123	22.51364677208277
82-83	22.103606561619507	28.179210431823403	27.571539250350174	22.145643756206915
84-85	21.85179807422343	27.840618446509268	27.55852107910232	22.74906240016498
86-87	21.79364358006958	28.162878907090892	27.61111188042656	22.432365632412964
88-89	22.177291600231737	27.82455901058121	27.564368270866364	22.433781118320688
90-91	22.193147515876465	28.101254675753175	27.686498781319184	22.01909902705118
92-93	21.86579481065666	27.9780472580453	27.703561784959696	22.452596146338347
94-95	22.09647341551857	27.66408622852432	27.80611721457693	22.43332314138018
96-97	21.984163741638472	28.098984645894888	27.894452063198667	22.022399549267973
98-99	22.20632392349081	28.463891626377553	28.10606442496303	21.223720025168603
100-101	25.728197847597745	35.73156868382222	29.007800947618666	9.532432520961368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2210.0
1	2006.5
2	2553.0
3	3457.5
4	4436.5
5	6433.5
6	8137.0
7	9724.0
8	11009.0
9	11263.0
10	10849.0
11	10351.5
12	11208.5
13	14485.0
14	22456.5
15	33312.0
16	41459.5
17	45112.5
18	46223.0
19	46410.0
20	46400.0
21	47032.0
22	49393.0
23	54908.5
24	64861.0
25	79664.0
26	97912.0
27	117915.0
28	138644.5
29	159479.5
30	178337.5
31	196948.5
32	216028.0
33	234113.5
34	252124.0
35	268673.5
36	286131.5
37	302415.5
38	315714.5
39	326804.5
40	338265.5
41	348715.5
42	354751.5
43	359819.5
44	364524.0
45	367569.0
46	367295.0
47	363686.0
48	358659.0
49	351432.0
50	342619.0
51	330481.5
52	313741.0
53	296319.5
54	277443.5
55	255992.0
56	233971.0
57	211489.0
58	187653.5
59	163026.0
60	137526.0
61	112755.0
62	90077.0
63	70115.5
64	52924.0
65	39630.0
66	29891.0
67	22758.5
68	17474.0
69	12875.5
70	9255.5
71	6688.5
72	4806.5
73	3391.0
74	2335.0
75	1602.5
76	1118.5
77	775.0
78	524.5
79	352.0
80	247.0
81	175.5
82	132.5
83	102.0
84	73.5
85	51.0
86	39.5
87	31.0
88	19.5
89	13.0
90	9.5
91	6.0
92	4.0
93	2.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002836492721290093
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.087520568967621E-4
14-15	0.0
16-17	0.0
18-19	4.278181170540843E-4
20-21	0.0
22-23	0.0015474588030124801
24-25	2.286640065029698E-4
26-27	0.00557253974423333
28-29	4.1084832078661256E-4
30-31	0.0
32-33	1.1788950205183731E-5
34-35	3.551642960436473E-4
36-37	0.02116859205156893
38-39	0.008865409568407716
40-41	0.014278340157798376
42-43	0.0019571049085291975
44-45	0.003179323559944259
46-47	0.0
48-49	8.121423916565567E-4
50-51	2.5680450070431842E-5
52-53	6.491185683871728E-6
54-55	0.004521687793142694
56-57	0.009312571479964222
58-59	0.012149279529560521
60-61	0.006284525058713192
62-63	0.007469492072477575
64-65	8.49771832780227E-4
66-67	9.895811943948141E-5
68-69	7.180021962251179E-6
70-71	1.0945295558734719E-4
72-73	7.415015762840509E-6
74-75	1.5823391659610815E-4
76-77	6.128969609121133E-5
78-79	7.795314376843738E-6
80-81	0.002278611460224733
82-83	0.002232013609460374
84-85	0.009750400454052432
86-87	0.011395759887814702
88-89	0.04608980026383028
90-91	5.602293859220658E-4
92-93	6.185499360742087E-4
94-95	0.0
96-97	0.00978847299916247
98-99	1.1045896805817855E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	279.0
20-21	902.0
22-23	1736.0
24-25	3664.0
26-27	4737.0
28-29	8636.0
30-31	22129.0
32-33	32202.0
34-35	47153.0
36-37	67210.0
38-39	70687.0
40-41	76740.0
42-43	87280.0
44-45	101720.0
46-47	102141.0
48-49	94141.0
50-51	87733.0
52-53	81514.0
54-55	90054.0
56-57	94107.0
58-59	56325.0
60-61	100125.0
62-63	96927.0
64-65	102766.0
66-67	109445.0
68-69	111229.0
70-71	110320.0
72-73	107030.0
74-75	109296.0
76-77	110236.0
78-79	116214.0
80-81	116366.0
82-83	111444.0
84-85	112026.0
86-87	117805.0
88-89	124630.0
90-91	133423.0
92-93	138744.0
94-95	165804.0
96-97	206906.0
98-99	691099.0
100-101	4408738.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.05056690471903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.84191077479349	80.8673230933319
2	5.843416983685092	10.290323561482714
3	1.3063899848866602	3.4508513630375335
4	0.4564702276079034	1.607698492640081
5	0.22146903341817537	0.9750236972155251
6	0.11263322721343678	0.5950451705070682
7	0.060327116439903766	0.37182857615823656
8	0.037492150583949065	0.2640964090715046
9	0.026889013912629305	0.2130833626664299
>10	0.0915343743093444	1.2274797702138391
>50	0.0012164291312611035	0.0703385124568923
>100	1.920407386246678E-4	0.03148588747872044
>500	5.864327960152354E-5	0.035422103739511795
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1721044302851625E-5	0.0	0.0	0.0
3	0.0	2.344208860570325E-5	0.0	1.1721044302851625E-5	0.0
4	0.0	3.516313290855487E-5	0.0	3.516313290855487E-5	0.0
5	0.0	3.516313290855487E-5	0.0	3.516313290855487E-5	0.0
6	0.0	3.516313290855487E-5	0.0	3.516313290855487E-5	0.0
7	0.0	3.516313290855487E-5	0.0	5.860522151425812E-5	0.0
8	0.0	5.860522151425812E-5	0.0	5.860522151425812E-5	0.0
9	0.0	9.3768354422813E-5	0.0	9.3768354422813E-5	0.0
10-11	0.0	1.0548939872566462E-4	0.0	1.2307096517994205E-4	0.0
12-13	0.0	1.0548939872566462E-4	0.0	1.4065253163421948E-4	0.0
14-15	5.860522151425812E-6	1.5823409808849695E-4	0.0	1.6409462023992273E-4	2.930261075712906E-5
16-17	1.1721044302851625E-5	1.6409462023992275E-4	0.0	1.8167618669420017E-4	3.516313290855487E-5
18-19	1.1721044302851625E-5	1.8167618669420017E-4	0.0	2.6372349681416153E-4	3.516313290855487E-5
20-21	1.1721044302851625E-5	2.0511827529990344E-4	0.0	4.1609707275123265E-4	3.516313290855487E-5
22-23	2.344208860570325E-5	2.1097879745132924E-4	0.0	7.50146835382504E-4	3.516313290855487E-5
24-25	4.68841772114065E-5	2.2269984175418087E-4	0.0	0.001406525316342195	3.516313290855487E-5
26-27	6.446574366568392E-5	2.3442088605703248E-4	0.0	0.003645244778186855	3.516313290855487E-5
28-29	7.032626581710974E-5	2.3442088605703248E-4	0.0	0.018396179033325626	3.516313290855487E-5
30-31	7.032626581710974E-5	2.5786297466273575E-4	0.0	0.02820083259266101	3.516313290855487E-5
32-33	9.3768354422813E-5	2.5786297466273575E-4	0.0	0.0367806370223484	3.516313290855487E-5
34-35	1.0548939872566462E-4	2.871655854198648E-4	0.0	0.04884745213213414	3.516313290855487E-5
36-37	1.1721044302851624E-4	3.1646819617699385E-4	0.0	0.06594845576999467	3.516313290855487E-5
38-39	1.1721044302851624E-4	3.1646819617699385E-4	0.0	0.0969037337738258	4.102365505998068E-5
40-41	1.2893148733136788E-4	3.633523733884004E-4	0.0	0.15753669595247727	4.68841772114065E-5
42-43	1.2893148733136788E-4	3.6921289553982616E-4	0.0	0.1830885725326938	4.68841772114065E-5
44-45	1.2893148733136788E-4	4.1023655059980687E-4	0.0	0.22192039230804123	4.68841772114065E-5
46-47	1.2893148733136788E-4	4.4539968350836175E-4	0.0	0.2662728239500318	4.68841772114065E-5
48-49	1.2893148733136788E-4	4.4539968350836175E-4	0.0	0.3025084324122976	5.860522151425812E-5
50-51	1.2893148733136788E-4	4.8056281641691657E-4	0.0	0.33674560282092714	5.860522151425812E-5
52-53	1.2893148733136788E-4	4.922838607197682E-4	0.0	0.38376457204181647	6.446574366568392E-5
54-55	1.2893148733136788E-4	4.98144382871194E-4	0.0	0.48349893801478094	7.032626581710974E-5
56-57	1.2893148733136788E-4	5.274469936283231E-4	0.0	0.588249910949366	7.032626581710974E-5
58-59	1.2893148733136788E-4	5.333075157797489E-4	0.0	0.6757475066701533	7.032626581710974E-5
60-61	1.2893148733136788E-4	5.450285600826006E-4	0.0	1.2076543576557115	7.032626581710974E-5
62-63	1.2893148733136788E-4	5.508890822340264E-4	0.0	1.2203189460249426	8.204731011996138E-5
64-65	1.2893148733136788E-4	6.212153480511361E-4	0.0	1.2334640972105908	8.204731011996138E-5
66-67	1.2893148733136788E-4	6.212153480511361E-4	0.0	1.2431398192825946	8.204731011996138E-5
68-69	1.2893148733136788E-4	6.387969145054135E-4	0.0	1.2529796359748386	8.204731011996138E-5
70-71	1.4065253163421948E-4	6.56378480959691E-4	0.0	1.262245121496243	9.3768354422813E-5
72-73	1.4065253163421948E-4	6.680995252625426E-4	0.0	1.269541471574768	1.1721044302851624E-4
74-75	1.4065253163421948E-4	6.915416138682459E-4	0.0	1.276134558995122	1.1721044302851624E-4
76-77	1.4065253163421948E-4	7.091231803225233E-4	0.0	1.2820126627130022	1.1721044302851624E-4
78-79	1.4065253163421948E-4	7.208442246253749E-4	0.0	1.2870820143739854	1.2307096517994207E-4
80-81	1.4065253163421948E-4	7.267047467768008E-4	0.0	1.2902759989465125	1.2893148733136788E-4
82-83	1.4065253163421948E-4	7.267047467768008E-4	0.0	1.2920517371583946	1.2893148733136788E-4
84-85	1.4065253163421948E-4	7.267047467768008E-4	0.0	1.2935871939620682	1.2893148733136788E-4
86-87	1.4065253163421948E-4	7.267047467768008E-4	0.0	1.2938274753702765	1.2893148733136788E-4
88-89	1.4065253163421948E-4	7.970310125939104E-4	0.0	1.2939095226803965	1.2893148733136788E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8395	0.0	45.036182	1
GTATCAA	14535	0.0	32.451237	1
ACCGAGG	8610	0.0	29.956726	92-93
AGACCGA	8455	0.0	29.518366	90-91
GACCGAG	8565	0.0	29.33275	90-91
GAGACCG	8680	0.0	28.716007	88-89
CGAGACC	8670	0.0	28.675035	88-89
ACGAGAC	9070	0.0	28.079643	86-87
CACGAGA	9450	0.0	26.48642	86-87
GCCCACG	9300	0.0	26.305304	82-83
CCCACGA	9495	0.0	25.991482	84-85
TCAACGC	17745	0.0	25.659136	4
CCACGAG	9860	0.0	25.37049	84-85
ATCAACG	18015	0.0	25.229193	3
CAACGCA	18420	0.0	24.785427	5
TATCAAC	18940	0.0	24.148527	2
CGAGCCC	10020	0.0	24.050219	80-81
AACGCAG	19030	0.0	24.012419	6
ATCTCCG	9935	0.0	23.661299	74-75
TCCGAGC	9925	0.0	23.648085	78-79
>>END_MODULE