FastQCFastQC Report
Fri 27 May 2016
SRR2139838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9763291
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG132350.045.1596894-95
GGTATCA90600.042.74081
CCGTCTT50100.034.10918492-93
TATGCCG47350.034.02298488-89
TGCCGTC50300.033.0359790-91
GTATCAA153300.031.465311
GCCGTCT49700.031.12894690-91
CTATCTC176750.030.93542994-95
CGTATGC55400.029.97977686-87
ATCTCGT101600.029.97694694-95
ATGCCGT51450.029.51220388-89
CGCTATC182900.027.35174292-93
GCTATCT214950.027.10751394-95
CTCGTAT60650.027.03190484-85
TCAACGC180200.026.0457954
TCGTATG60000.025.85039184-85
ATCAACG182400.025.6397483
CAACGCA186600.025.0851065
TCTCGTA62650.024.5243182-83
AACGCAG194600.024.0969266
Produced by FastQC (version 0.11.5)