##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9763291
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.671314109146188	31.0	31.0	34.0	30.0	34.0
2	31.907817456224546	31.0	31.0	34.0	30.0	34.0
3	31.92693068351645	31.0	31.0	34.0	30.0	34.0
4	35.66878115176532	37.0	35.0	37.0	33.0	37.0
5	35.49647388365255	37.0	35.0	37.0	33.0	37.0
6	35.48665895546901	37.0	35.0	37.0	33.0	37.0
7	35.35013460112989	37.0	35.0	37.0	32.0	37.0
8	35.32924727942658	37.0	35.0	37.0	32.0	37.0
9	36.86212138919141	39.0	37.0	39.0	33.0	39.0
10-11	36.789535618676126	39.0	37.0	39.0	32.0	39.0
12-13	36.721565146424496	39.0	35.0	39.0	32.0	39.0
14-15	37.80515437878478	40.0	37.0	41.0	32.0	41.0
16-17	37.84331082623677	40.0	37.0	41.0	33.0	41.0
18-19	37.86607164530894	40.0	37.0	41.0	32.5	41.0
20-21	37.83873845139097	40.0	37.0	41.0	32.0	41.0
22-23	37.7703042794112	39.5	37.0	41.0	32.0	41.0
24-25	37.67673236111322	39.0	37.0	41.0	32.0	41.0
26-27	37.511641318446124	39.0	36.0	41.0	32.0	41.0
28-29	37.36795142291818	39.0	36.0	41.0	31.5	41.0
30-31	37.225709284932336	39.0	36.0	40.0	31.0	41.0
32-33	37.45985809392028	39.0	36.0	41.0	32.0	41.0
34-35	37.46590766400813	39.0	36.0	41.0	32.0	41.0
36-37	37.35383404577779	39.0	36.0	41.0	31.0	41.0
38-39	37.240156889159785	39.0	36.0	41.0	31.0	41.0
40-41	37.22646689104985	39.0	36.0	41.0	31.0	41.0
42-43	37.153078223783126	39.0	36.0	40.5	31.0	41.0
44-45	37.12538130651834	39.0	36.0	40.0	31.0	41.0
46-47	37.13184684952439	39.0	36.0	41.0	31.0	41.0
48-49	37.054350799878236	39.0	36.0	41.0	31.0	41.0
50-51	36.97515819772897	39.0	35.0	40.0	31.0	41.0
52-53	36.8509253985481	39.0	35.0	40.0	30.0	41.0
54-55	36.74947343299057	39.0	35.0	40.0	30.0	41.0
56-57	36.57884269842015	38.5	35.0	40.0	30.0	41.0
58-59	36.444113521399025	38.0	35.0	40.0	30.0	41.0
60-61	36.29085941982868	38.0	34.5	40.0	29.5	41.0
62-63	36.07086030379244	38.0	34.0	40.0	29.0	41.0
64-65	35.88649897603517	37.5	34.0	40.0	29.0	41.0
66-67	35.63829287660353	37.0	34.0	40.0	29.0	41.0
68-69	35.36546841972509	36.5	34.0	39.0	29.0	41.0
70-71	35.07866494110751	36.0	34.0	39.0	29.0	40.5
72-73	34.77473862774645	36.0	33.0	39.0	28.0	40.0
74-75	34.42851511199159	35.0	33.0	38.0	28.0	40.0
76-77	33.3291935497861	34.5	31.5	37.0	26.5	39.0
78-79	33.644117726106806	35.0	32.0	37.0	27.5	39.0
80-81	33.53350798205393	35.0	32.0	36.0	28.0	39.0
82-83	33.33423126426014	35.0	32.0	36.0	27.5	37.5
84-85	33.120234489587844	35.0	32.0	35.5	27.0	37.0
86-87	32.961676014876275	34.5	32.0	35.0	27.0	37.0
88-89	32.761160119437065	34.0	32.0	35.0	27.0	36.0
90-91	32.68301635358437	34.0	32.0	35.0	27.0	36.0
92-93	32.66316256833946	34.0	32.0	35.0	27.0	36.0
94-95	32.63926705631484	34.0	32.0	35.0	27.0	35.0
96-97	32.53004273207137	34.0	32.0	35.0	27.0	35.0
98-99	32.661872213126436	34.0	32.0	35.0	28.5	35.0
100-101	32.179685908819984	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	12.0
20	77.0
21	575.0
22	2413.0
23	8545.0
24	21682.0
25	45609.0
26	80026.0
27	125287.0
28	177067.0
29	233975.0
30	293176.0
31	360206.0
32	440124.0
33	544292.0
34	692610.0
35	901327.0
36	1252258.0
37	1814554.0
38	1935992.0
39	805716.0
40	27767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.94601635862334	22.281943660185892	14.641313057246782	25.130726923943985
2	14.548540425045925	22.991412939435797	39.49876804591238	22.9612785896059
3	17.19069932464371	26.995948394859887	31.443926028631125	24.369426251865278
4	12.32648909061504	18.833209007085827	38.67570883629301	30.164593066006123
5	12.65573258033587	38.1815414494969	36.30351691862918	12.85920905153805
6	29.964496602631225	38.84714693027177	18.36781265661343	12.820543810483576
7	25.704304009785222	32.73898114887695	24.10270266450114	17.454012176836684
8	24.059960929158006	37.21302581271008	21.59204309284646	17.134970165285456
9	25.25099374790734	16.03875168731527	21.486453696811864	37.22380086796552
10-11	23.76727273621159	26.984051791552666	29.718201577726198	19.530473894509544
12-13	24.341294282744826	24.629166116556675	29.634753936696363	21.394785664002136
14-15	21.497541146730136	26.137999983816933	26.949237710931694	25.41522115852124
16-17	21.3787031442574	29.031839776157447	27.782215033844633	21.80724204574052
18-19	21.407941219564368	28.445204482913656	29.153186052777414	20.993668244744565
20-21	22.075605175374445	27.765262882035863	28.93146394678049	21.2276679958092
22-23	21.77391558764156	27.696443574835293	28.80457033459139	21.725070502931757
24-25	21.619467967397565	27.7773570846107	28.89599843263047	21.70717651536127
26-27	21.681475353199218	27.910862881506926	28.771166067450515	21.636495697843333
28-29	21.615686440222508	27.98195144905745	28.795998820093367	21.606363290626675
30-31	21.83630461036396	27.850053888077834	28.700766120093828	21.61287538146438
32-33	21.651580249803036	27.989002830225157	28.66889423829628	21.69052268167553
34-35	21.76334203378991	27.990939796606433	28.6700157472355	21.575702422368153
36-37	21.74930820525603	27.941935723374854	28.6358621861473	21.672893885221814
38-39	21.62618807262311	28.025218074158047	28.696966633121495	21.651627220097346
40-41	21.7558354186496	27.828384518592376	28.66408385514952	21.7516962076085
42-43	21.783537472023166	27.955297624090715	28.472628984250107	21.78853591963601
44-45	21.749428100331528	27.9896379807488	28.307638450374046	21.953295468545626
46-47	21.782333425120072	27.917926698818622	28.196109447352107	22.1036304287092
48-49	21.642820301845926	28.157996657186104	28.245404838789558	21.953778202178416
50-51	21.649797148446137	28.227027916174226	28.16792947567602	21.95524545970362
52-53	21.72438066772741	28.058655734921977	27.85164326152242	22.365320335828194
54-55	21.67626756137499	27.756126063648868	28.074954467498998	22.49265190747715
56-57	21.731612199362782	27.415181856270515	28.40859386972513	22.444612074641572
58-59	21.532758963659308	27.163787392460392	28.56282617422722	22.74062746965308
60-61	21.559023409739176	26.885146339758588	28.93886023935867	22.616970011143568
62-63	21.281540529345243	27.53503711697327	28.93059937684707	22.252822976834416
64-65	20.818976466028968	27.8351112329173	28.720466918213567	22.625445382840166
66-67	20.908383376163663	28.585730580853756	28.060811992953994	22.445074050028587
68-69	21.040942477622192	28.979971193297317	27.475910803491782	22.503175525588706
70-71	21.01791147663524	28.50805740831491	27.459652383239813	23.01437873181003
72-73	20.903486215690528	27.963740935972055	27.434635916012372	23.69813693232504
74-75	21.458773797557644	27.515854214168527	27.489576235114978	23.535795753158855
76-77	21.961385750514843	27.32632903521343	27.371705843838946	23.34057937043278
78-79	22.24951573347064	27.194154956518553	26.542384704065363	24.013944605945444
80-81	22.12562925863145	27.690628205355367	26.405158887189213	23.77858364882397
82-83	22.01911314611986	27.876119640117693	26.488250411238447	23.616516802524004
84-85	22.047346285136214	27.872287324302352	26.479337836659756	23.601028553901678
86-87	22.455135860908975	28.301279490486568	26.37165025669015	22.8719343919143
88-89	22.701153702646952	27.854999416937098	26.783990821115527	22.659856059300424
90-91	22.323618192038595	27.865284666723493	27.174625462772045	22.636471678465867
92-93	22.054093575324902	27.21001011304745	27.06866306396254	23.66723324766511
94-95	21.818962854536775	27.235832191285535	27.889494156610155	23.055710797567535
96-97	21.2644925922338	27.560883605417107	28.245054195076108	22.929569607272985
98-99	21.527402538187726	27.540537141447903	28.706174803863778	22.225885516500597
100-101	25.500873462503566	35.13087293704782	29.81315310488355	9.555100495565068
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2223.0
1	2000.0
2	2533.0
3	3483.0
4	4555.0
5	6745.5
6	8629.5
7	10281.5
8	11527.0
9	11760.0
10	11356.5
11	10758.0
12	11628.0
13	15022.0
14	22909.5
15	33666.0
16	42044.0
17	46130.5
18	47288.5
19	47008.5
20	46164.5
21	45802.5
22	46999.0
23	52348.5
24	62068.5
25	75871.0
26	94243.0
27	114570.5
28	136685.0
29	160140.5
30	181867.5
31	203495.5
32	225736.0
33	246816.5
34	267078.0
35	286337.0
36	308881.0
37	329443.0
38	345957.0
39	362216.0
40	377107.5
41	389582.5
42	398417.0
43	407079.5
44	414484.5
45	419035.5
46	421261.0
47	420845.0
48	417845.0
49	410625.0
50	400494.0
51	386080.5
52	366556.5
53	345352.0
54	321516.0
55	296338.5
56	269373.0
57	239963.0
58	210352.5
59	180644.5
60	151037.0
61	122492.0
62	96852.5
63	74767.5
64	55925.5
65	42236.5
66	32372.5
67	24665.5
68	18987.5
69	14097.0
70	10122.5
71	7379.0
72	5327.0
73	3798.0
74	2723.5
75	1939.0
76	1313.0
77	858.0
78	586.0
79	403.5
80	278.5
81	206.5
82	156.5
83	114.5
84	83.5
85	66.0
86	57.0
87	43.5
88	26.5
89	13.5
90	5.5
91	2.5
92	2.0
93	2.5
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0035438869946619435
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.423052124534646E-4
14-15	0.0
16-17	0.0
18-19	4.3530403836165485E-4
20-21	0.0
22-23	0.0014700433340091093
24-25	1.2296293958482382E-4
26-27	0.005346013962271288
28-29	3.8974510875016744E-4
30-31	0.0
32-33	0.0
34-35	2.5841338420080207E-4
36-37	0.02236764454460011
38-39	0.009444491559227938
40-41	0.01389549628483714
42-43	0.0015775935398766247
44-45	0.002806296987029703
46-47	0.0
48-49	7.412071013350348E-4
50-51	1.1099339644787833E-5
52-53	1.119046706491674E-5
54-55	0.004366400286355145
56-57	0.008624730077112163
58-59	0.01137936141588731
60-61	0.0061022854923756175
62-63	0.006631525114598361
64-65	8.577101214488556E-4
66-67	1.1753912333797476E-4
68-69	0.0
70-71	1.5143773470955907E-4
72-73	0.0
74-75	1.8734265558713876E-4
76-77	6.343741065237193E-5
78-79	0.0
80-81	0.002086821212183256
82-83	0.002163750934369761
84-85	0.009443985040999464
86-87	0.010873085978927378
88-89	0.044882391914879576
90-91	4.319571083869656E-4
92-93	5.374943544685439E-4
94-95	0.0
96-97	0.0108076322439946
98-99	1.0651217049026241E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	305.0
20-21	994.0
22-23	1912.0
24-25	3988.0
26-27	4711.0
28-29	8823.0
30-31	23272.0
32-33	33822.0
34-35	49664.0
36-37	70483.0
38-39	50359.0
40-41	80755.0
42-43	90202.0
44-45	105159.0
46-47	106877.0
48-49	98803.0
50-51	93073.0
52-53	15742.0
54-55	15735.0
56-57	15708.0
58-59	16107.0
60-61	110920.0
62-63	109882.0
64-65	117290.0
66-67	124778.0
68-69	127901.0
70-71	126072.0
72-73	122517.0
74-75	124649.0
76-77	126563.0
78-79	133583.0
80-81	133395.0
82-83	128235.0
84-85	128578.0
86-87	136237.0
88-89	143716.0
90-91	152972.0
92-93	160569.0
94-95	193947.0
96-97	241553.0
98-99	875130.0
100-101	5358310.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.8965593437609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.50043195122817	80.42573146980882
2	6.202868590200564	10.904216142802287
3	1.3422301087600381	3.5393222522282803
4	0.456765731019635	1.605925447310547
5	0.19714568182711295	0.8664213561041519
6	0.09617817942195574	0.5072238633082089
7	0.059711833931259645	0.3673925328264631
8	0.03295453250975835	0.2317272017911898
9	0.023635480753382076	0.18697296929921697
>10	0.08603227053141484	1.17701281786099
>50	0.0016661776821709812	0.09875308764519507
>100	3.283751090621386E-4	0.0539017518932697
>500	5.108702551467156E-5	0.035399107121421636
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0484895922901408E-5	0.0	0.0	0.0
3	0.0	2.0484895922901408E-5	0.0	0.0	0.0
4	0.0	6.145468776870422E-5	0.0	0.0	0.0
5	0.0	6.145468776870422E-5	0.0	0.0	0.0
6	1.0242447961450704E-5	6.145468776870422E-5	0.0	0.0	0.0
7	1.0242447961450704E-5	6.145468776870422E-5	0.0	0.0	0.0
8	1.0242447961450704E-5	6.145468776870422E-5	0.0	0.0	0.0
9	1.0242447961450704E-5	2.1509140719046477E-4	0.0	2.0484895922901408E-5	0.0
10-11	1.0242447961450704E-5	2.1509140719046477E-4	0.0	3.072734388435211E-5	0.0
12-13	1.0242447961450704E-5	2.1509140719046477E-4	0.0	3.072734388435211E-5	0.0
14-15	1.0242447961450704E-5	2.3557630311336618E-4	0.0	3.072734388435211E-5	0.0
16-17	4.0969791845802816E-5	2.3557630311336618E-4	0.0	4.609101582652817E-5	1.0242447961450704E-5
18-19	4.0969791845802816E-5	2.867885429206197E-4	0.0	9.73032556337817E-5	1.0242447961450704E-5
20-21	4.0969791845802816E-5	3.6360690263149997E-4	0.0	2.8166731893989434E-4	1.0242447961450704E-5
22-23	4.609101582652817E-5	4.0969791845802816E-4	0.0	7.937897170124295E-4	1.0242447961450704E-5
24-25	5.121223980725352E-5	4.3018281438092954E-4	0.0	0.0015107610743139788	1.0242447961450704E-5
26-27	5.121223980725352E-5	4.5066771030383097E-4	0.0	0.004117464080503183	2.0484895922901408E-5
28-29	5.121223980725352E-5	4.5578893428455634E-4	0.0	0.019383832767045455	3.072734388435211E-5
30-31	5.121223980725352E-5	5.172436220532606E-4	0.0	0.034168806399399544	3.072734388435211E-5
32-33	5.121223980725352E-5	5.274860700147112E-4	0.0	0.054469338258994845	3.072734388435211E-5
34-35	6.145468776870422E-5	6.196681016677676E-4	0.0	0.08393173982010779	3.072734388435211E-5
36-37	6.145468776870422E-5	7.067289093400984E-4	0.0	0.12191585808514771	3.072734388435211E-5
38-39	6.145468776870422E-5	7.323350292437253E-4	0.0	0.19413535866133663	3.072734388435211E-5
40-41	6.145468776870422E-5	7.886684930317042E-4	0.0	0.5540703436986565	3.072734388435211E-5
42-43	6.145468776870422E-5	8.603656287618591E-4	0.0	0.6199036779708809	3.072734388435211E-5
44-45	6.657591174942957E-5	9.525476604149154E-4	0.0	0.7271369869032891	3.072734388435211E-5
46-47	7.169713573015493E-5	0.0010447296920679718	0.0	0.8458469587765027	3.072734388435211E-5
48-49	7.681835971088029E-5	0.0010498509160486971	0.0	0.945208946450536	3.072734388435211E-5
50-51	8.193958369160563E-5	0.0011266692757595773	0.0	1.0404841973879504	3.072734388435211E-5
52-53	8.193958369160563E-5	0.0011420329477017533	0.0	1.316758867476141	4.0969791845802816E-5
54-55	8.706080767233098E-5	0.0011471541716824788	0.0	2.224152696053001	5.121223980725352E-5
56-57	9.218203165305634E-5	0.00116763906760538	0.0	3.2278152930195363	5.121223980725352E-5
58-59	1.0242447961450704E-4	0.00116763906760538	0.0	4.254492670555451	5.121223980725352E-5
60-61	1.0242447961450704E-4	0.001177881515566831	0.0	5.035084993369551	5.121223980725352E-5
62-63	1.0242447961450704E-4	0.001177881515566831	0.0	5.0577361670363	5.121223980725352E-5
64-65	1.0242447961450704E-4	0.0012495786512969858	0.0	5.0713842289449325	5.121223980725352E-5
66-67	1.0242447961450704E-4	0.0012854272191620633	0.0	5.081493525082885	5.121223980725352E-5
68-69	1.1266692757595774E-4	0.0013059121150849648	0.0	5.090404454809347	5.633346378797887E-5
70-71	1.1266692757595774E-4	0.0013468819069307673	0.0	5.098890322945408	6.145468776870422E-5
72-73	1.1266692757595774E-4	0.0013622455788729435	0.0	5.106034430398521	8.193958369160563E-5
74-75	1.177881515566831E-4	0.0013776092508151197	0.0	5.112210626519275	8.193958369160563E-5
76-77	1.2290937553740845E-4	0.0014339427146030986	0.0	5.1179771247215715	8.193958369160563E-5
78-79	1.2290937553740845E-4	0.0014544276105259999	0.0	5.12332880378143	8.193958369160563E-5
80-81	1.2290937553740845E-4	0.0014544276105259999	0.0	5.126073779835099	8.193958369160563E-5
82-83	1.2290937553740845E-4	0.0014595488345067252	0.0	5.127825238436507	9.218203165305634E-5
84-85	1.4339427146030985E-4	0.0014697912824681758	0.0	5.129699606413452	9.218203165305634E-5
86-87	1.4339427146030985E-4	0.0015056398503332535	0.0	5.130042728420161	9.73032556337817E-5
88-89	1.4339427146030985E-4	0.0015056398503332535	0.0	5.130093940659968	1.0242447961450704E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	13235	0.0	45.15968	94-95
GGTATCA	9060	0.0	42.7408	1
CCGTCTT	5010	0.0	34.109184	92-93
TATGCCG	4735	0.0	34.022984	88-89
TGCCGTC	5030	0.0	33.03597	90-91
GTATCAA	15330	0.0	31.46531	1
GCCGTCT	4970	0.0	31.128946	90-91
CTATCTC	17675	0.0	30.935429	94-95
CGTATGC	5540	0.0	29.979776	86-87
ATCTCGT	10160	0.0	29.976946	94-95
ATGCCGT	5145	0.0	29.512203	88-89
CGCTATC	18290	0.0	27.351742	92-93
GCTATCT	21495	0.0	27.107513	94-95
CTCGTAT	6065	0.0	27.031904	84-85
TCAACGC	18020	0.0	26.045795	4
TCGTATG	6000	0.0	25.850391	84-85
ATCAACG	18240	0.0	25.639748	3
CAACGCA	18660	0.0	25.085106	5
TCTCGTA	6265	0.0	24.52431	82-83
AACGCAG	19460	0.0	24.096926	6
>>END_MODULE