FastQCFastQC Report
Fri 27 May 2016
SRR2139837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8418641
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75050.042.212031
GTATCAA127650.031.5835821
TCAACGC152400.025.6211784
ATCAACG154000.025.3284053
CAACGCA157050.024.9146985
AACGCAG164550.023.8537356
TATCAAC167550.023.5737612
GTACATG182350.021.3237611
TACATGG186000.020.707262
ACGCAGA196500.019.9547547
ACATGGG189650.019.7255443
CGCAGAG199650.019.7014188
CATGGGG135400.017.2907184
AGAGTAC215000.016.33398810-11
GCAGAGT244850.015.7970179
GAGTACT145600.015.53153312-13
CAGAGTA238900.014.61424110-11
AGTACTT167400.013.90011512-13
GAGTACA137050.013.4991181
ATGGGGG82200.013.4439735
Produced by FastQC (version 0.11.5)