##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8418641
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62930347071457	31.0	31.0	34.0	30.0	34.0
2	31.86631904127994	31.0	31.0	34.0	30.0	34.0
3	31.883125554350162	31.0	31.0	34.0	30.0	34.0
4	35.63366783308612	37.0	35.0	37.0	33.0	37.0
5	35.456739038996915	37.0	35.0	37.0	33.0	37.0
6	35.445734293694194	37.0	35.0	37.0	33.0	37.0
7	35.30961648085481	37.0	35.0	37.0	32.0	37.0
8	35.28793495292174	37.0	35.0	37.0	32.0	37.0
9	36.80896370328655	39.0	37.0	39.0	32.0	39.0
10-11	36.733378700909086	39.0	35.0	39.0	32.0	39.0
12-13	36.66749983756286	39.0	35.0	39.0	32.0	39.0
14-15	37.74042538457216	39.0	37.0	41.0	32.0	41.0
16-17	37.77791219509182	39.0	37.0	41.0	32.0	41.0
18-19	37.7988776335753	39.5	37.0	41.0	32.0	41.0
20-21	37.77229871141072	39.5	37.0	41.0	32.0	41.0
22-23	37.703760567578605	39.0	37.0	41.0	32.0	41.0
24-25	37.608704175411354	39.0	36.5	41.0	32.0	41.0
26-27	37.43879302196653	39.0	36.0	41.0	32.0	41.0
28-29	37.29454122992391	39.0	36.0	40.5	31.0	41.0
30-31	37.157151208862544	39.0	36.0	40.0	31.0	41.0
32-33	37.388342142474855	39.0	36.0	40.5	32.0	41.0
34-35	37.39199054509723	39.0	36.0	41.0	31.0	41.0
36-37	37.2799620137116	39.0	36.0	41.0	31.0	41.0
38-39	37.16718209329058	39.0	36.0	41.0	31.0	41.0
40-41	37.149794717754666	39.0	36.0	40.5	31.0	41.0
42-43	37.07641599647927	39.0	36.0	40.0	31.0	41.0
44-45	37.04903263437235	39.0	35.5	40.0	31.0	41.0
46-47	37.062897159586925	39.0	36.0	40.0	31.0	41.0
48-49	36.987457908793445	39.0	35.0	40.0	31.0	41.0
50-51	36.906680733893324	39.0	35.0	40.0	30.0	41.0
52-53	36.780590648347484	39.0	35.0	40.0	30.0	41.0
54-55	36.67213379649843	39.0	35.0	40.0	30.0	41.0
56-57	36.4958864955246	38.0	35.0	40.0	30.0	41.0
58-59	36.35405673591305	38.0	35.0	40.0	30.0	41.0
60-61	36.19409519489302	38.0	34.0	40.0	29.0	41.0
62-63	35.970833714306025	38.0	34.0	40.0	29.0	41.0
64-65	35.765729008704746	37.5	34.0	40.0	29.0	41.0
66-67	35.517206021235545	37.0	34.0	40.0	28.5	41.0
68-69	35.24606207855626	36.5	34.0	39.0	28.0	41.0
70-71	34.967041605973876	36.0	33.0	39.0	28.0	40.0
72-73	34.66416358921798	36.0	33.0	39.0	28.0	40.0
74-75	34.34240035948545	35.0	33.0	38.0	28.0	40.0
76-77	33.28391164916736	34.5	31.5	36.5	26.5	39.0
78-79	33.651702428215	35.0	32.0	37.0	27.5	39.0
80-81	33.578461316658604	35.0	32.0	36.0	28.0	39.0
82-83	33.38438035486118	35.0	32.0	36.0	28.0	37.5
84-85	33.152335487421894	35.0	32.0	35.5	27.5	37.0
86-87	32.97998143903497	34.0	32.0	35.0	27.0	37.0
88-89	32.792396091629385	34.0	32.0	35.0	27.0	36.0
90-91	32.69628652667795	34.0	32.0	35.0	27.0	36.0
92-93	32.65752058181103	34.0	32.0	35.0	27.0	36.0
94-95	32.646786816076066	34.0	32.0	35.0	27.5	35.5
96-97	32.5556744333821	34.0	32.0	35.0	27.0	35.0
98-99	32.69137179856211	34.0	32.0	35.0	28.5	35.0
100-101	32.245944938408684	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	92.0
21	494.0
22	2331.0
23	7612.0
24	19645.0
25	40457.0
26	71109.0
27	110298.0
28	153783.0
29	202416.0
30	254645.0
31	312370.0
32	382203.0
33	471313.0
34	598161.0
35	776764.0
36	1071335.0
37	1531373.0
38	1627234.0
39	761281.0
40	23714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.659427453908535	22.641385943408203	14.534032274330263	25.165154328352994
2	14.503055101588453	23.43382537586219	39.27308799866198	22.79003152388738
3	17.032701596374046	27.368134595595656	31.63541478963172	23.963749018398577
4	12.219549449845884	19.152164820901614	38.91275325791894	29.715532471333557
5	12.469886766759624	38.36033630606175	36.52331771838234	12.64645920879629
6	29.573799381634164	39.28093619860973	18.496500801020023	12.648763618736089
7	25.35183529028022	32.9219882401447	24.437530950660562	17.288645518914514
8	23.925061063893803	37.35806052307017	21.762194159366103	16.95468425366992
9	25.20963894291252	15.905393756545742	21.59660923894961	37.28835806159213
10-11	23.588397462250736	27.094343374423495	29.984863352648013	19.332395810677756
12-13	24.14474355892802	24.7574722148526	29.849909809520536	21.247874416698842
14-15	21.32453444683055	26.333656465455647	27.046289300137634	25.295519787576165
16-17	21.225908077087503	29.201732203570625	27.901474834239874	21.670884885102
18-19	21.259509221510527	28.643518055753773	29.242584088609934	20.854388634125762
20-21	21.91290259315315	27.97108695473989	28.987526678780885	21.128483773326078
22-23	21.61037947002723	27.91297960074008	28.882841152283323	21.593799776949368
24-25	21.47945131247284	28.00833329669205	28.924355403624304	21.587859987210805
26-27	21.512073034880444	28.133351397344175	28.827081939173542	21.52749362860184
28-29	21.48644523344188	28.207526788080905	28.824139229019117	21.4818887494581
30-31	21.67274744279493	28.091208127540888	28.738009476087097	21.498034953577086
32-33	21.514748226510466	28.2119530898229	28.72291851304063	21.550380170626006
34-35	21.63641070269659	28.243184470364774	28.697664046217415	21.42274078072122
36-37	21.62927261503827	28.150271589882774	28.690476829742074	21.52997896533688
38-39	21.48258755303381	28.249245845100802	28.745658299182665	21.522508302682724
40-41	21.668190411018543	28.115444022783198	28.668596947237972	21.547768618960287
42-43	21.56661396143026	28.240682131241858	28.46465772967028	21.72804617765761
44-45	21.650222887057026	28.27782873691122	28.33223170297669	21.73971667305506
46-47	21.67363585478722	28.254243225435694	28.20449963884143	21.86762128093566
48-49	21.62318370580256	28.32007988281632	28.22535362919158	21.831382782189543
50-51	21.61705322190749	28.37713713494076	28.150846346998833	21.854963296152913
52-53	21.731383605362613	28.306292070543336	28.027602831459596	21.93472149263445
54-55	21.689658002893676	28.23784116252026	28.081053594437478	21.991447240148588
56-57	21.68198939835035	28.266417068843673	28.0681959897175	21.98339754308848
58-59	21.660507556660612	28.260308736664552	28.052327999618086	22.026855707056754
60-61	21.7210901716331	28.198236070538734	28.07790152853839	22.002772229289775
62-63	21.670294703210757	28.323147007966387	28.053709770511492	21.952848518311363
64-65	21.6353613197561	28.2825878533963	27.994667897688203	22.087382929159393
66-67	21.688102779728922	28.43186232420079	27.815349780888226	22.064685115182066
68-69	21.69401043640056	28.389411110476388	27.812324034382296	22.104254418740755
70-71	21.693672452395383	28.23151751621069	27.847422339930418	22.22738769146351
72-73	21.738878167150997	28.243395532791777	27.77058382402687	22.247142476030362
74-75	21.69347539047323	28.30849367448397	27.716328931880525	22.28170200316228
76-77	21.863513505528445	28.27659859659315	27.66224656433401	22.197641333544397
78-79	21.849250752246217	28.249227777219136	27.626821318768137	22.27470015176651
80-81	21.87112072831373	28.300724586003522	27.563261566127228	22.264893119555524
82-83	21.83264987018633	28.3346615141787	27.67331282451365	22.159375791121317
84-85	21.95826250468278	28.332494104567708	27.631253888034145	22.077989502715365
86-87	21.869032717924767	28.34774560848504	27.658116815545853	22.125104858044338
88-89	21.81551374386722	28.339443177665125	27.71667643598298	22.12836664248467
90-91	21.870393947843443	28.359837041744306	27.72692278207971	22.04284622833254
92-93	21.924640146795248	28.31362846643714	27.737170531009923	22.024560855757684
94-95	21.768211683365767	28.357586480758822	27.864139474902476	22.01006236097293
96-97	21.656898286192487	28.361499301276965	28.00222620440625	21.9793762081243
98-99	21.834489486564166	28.9564332593425	28.114411628934317	21.094665625159017
100-101	25.501704462794677	36.153690126267605	28.91006698297187	9.43453842796584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3791.0
1	3095.5
2	3009.0
3	3795.0
4	4694.0
5	6539.5
6	8136.0
7	9518.5
8	10382.0
9	10266.0
10	9739.5
11	9262.5
12	10236.0
13	13318.0
14	20066.5
15	29218.5
16	36163.0
17	39459.0
18	40783.0
19	41389.5
20	41719.5
21	42750.5
22	45585.0
23	52017.0
24	62835.0
25	77412.5
26	94739.5
27	113927.5
28	134873.5
29	156564.5
30	177293.5
31	197426.0
32	217380.5
33	235901.0
34	253147.0
35	269433.0
36	287511.5
37	303792.5
38	316839.5
39	328992.5
40	339400.0
41	348460.5
42	354934.0
43	360654.0
44	364933.5
45	366093.0
46	365413.0
47	363136.0
48	358490.0
49	350785.5
50	340395.5
51	327096.0
52	310548.0
53	292283.5
54	272053.5
55	249355.0
56	226436.0
57	203337.5
58	178883.5
59	152975.5
60	127699.5
61	104191.5
62	82834.5
63	64555.5
64	48587.0
65	36436.0
66	27500.5
67	20864.5
68	16107.5
69	11998.5
70	8804.0
71	6475.0
72	4621.0
73	3236.0
74	2271.5
75	1590.5
76	1109.0
77	765.0
78	540.0
79	393.0
80	275.0
81	189.5
82	147.0
83	103.5
84	72.0
85	54.5
86	44.5
87	33.5
88	23.5
89	16.0
90	9.5
91	7.5
92	6.5
93	4.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0031240196606554436
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.255489217321417E-4
14-15	0.0
16-17	0.0
18-19	4.335616639312687E-4
20-21	0.0
22-23	0.001556364020000347
24-25	2.4955620588054244E-4
26-27	0.0053796787006215345
28-29	3.866453182932667E-4
30-31	0.0
32-33	0.0
34-35	1.6190269000719806E-4
36-37	0.021197297435060658
38-39	0.009377212607133286
40-41	0.013518720669204263
42-43	0.0018190957534880235
44-45	0.003033065668936067
46-47	0.0
48-49	7.306301768592887E-4
50-51	1.945192641180623E-5
52-53	6.5540007717991305E-6
54-55	0.004372317690748487
56-57	0.009298710308434936
58-59	0.012092888593873407
60-61	0.006247894861966594
62-63	0.007279484439579059
64-65	8.268619606253989E-4
66-67	9.324354410390946E-5
68-69	0.0
70-71	8.88611313043918E-5
72-73	0.0
74-75	2.3705191834647266E-4
76-77	4.66495877342684E-5
78-79	7.911510076257464E-6
80-81	0.002151495240844179
82-83	0.002314822795389858
84-85	0.010382340124292158
86-87	0.01126082864536746
88-89	0.04645548556281712
90-91	4.6190775542233065E-4
92-93	5.818824533800779E-4
94-95	9.325980605877252E-6
96-97	0.009795033388350537
98-99	1.1150309517387236E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	270.0
20-21	954.0
22-23	1751.0
24-25	3285.0
26-27	4945.0
28-29	8853.0
30-31	20133.0
32-33	30164.0
34-35	44349.0
36-37	64144.0
38-39	66846.0
40-41	72630.0
42-43	81808.0
44-45	96193.0
46-47	97435.0
48-49	91726.0
50-51	85134.0
52-53	78912.0
54-55	88005.0
56-57	93162.0
58-59	97267.0
60-61	97133.0
62-63	93764.0
64-65	101782.0
66-67	108612.0
68-69	109611.0
70-71	109491.0
72-73	105161.0
74-75	107591.0
76-77	108898.0
78-79	114794.0
80-81	114955.0
82-83	110097.0
84-85	110538.0
86-87	115079.0
88-89	122734.0
90-91	128358.0
92-93	134057.0
94-95	161559.0
96-97	203991.0
98-99	644959.0
100-101	4387511.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.75476438138715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.19647079365366	81.82876042086173
2	5.670561083840677	10.065786258130851
3	1.2113446519429325	3.225378275035451
4	0.42174575175765044	1.4972777930440504
5	0.1961326667115006	0.8703854310736183
6	0.1008505593606598	0.5370580580271874
7	0.05969106552493488	0.37085065194376754
8	0.03764341686711244	0.26728260756407196
9	0.0240066851893367	0.19176369188019432
>10	0.08057636003082702	1.0755509028330623
>50	8.301587961582254E-4	0.04826171409739059
>100	1.4680632455578766E-4	0.021644195508735944
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1878401751541608E-5
2	0.0	0.0	0.0	0.0	1.1878401751541608E-5
3	0.0	0.0	0.0	0.0	1.1878401751541608E-5
4	0.0	2.3756803503083216E-5	0.0	1.1878401751541608E-5	1.1878401751541608E-5
5	0.0	3.563520525462483E-5	0.0	1.1878401751541608E-5	1.1878401751541608E-5
6	1.1878401751541608E-5	3.563520525462483E-5	0.0	1.1878401751541608E-5	3.563520525462483E-5
7	1.1878401751541608E-5	3.563520525462483E-5	0.0	1.1878401751541608E-5	3.563520525462483E-5
8	1.1878401751541608E-5	3.563520525462483E-5	0.0	1.1878401751541608E-5	3.563520525462483E-5
9	2.3756803503083216E-5	3.563520525462483E-5	0.0	3.563520525462483E-5	3.563520525462483E-5
10-11	2.3756803503083216E-5	3.563520525462483E-5	0.0	4.157440613039563E-5	3.563520525462483E-5
12-13	2.3756803503083216E-5	3.563520525462483E-5	0.0	5.345280788193724E-5	3.563520525462483E-5
14-15	3.563520525462483E-5	5.939200875770804E-5	0.0	5.939200875770804E-5	4.751360700616643E-5
16-17	4.751360700616643E-5	6.533120963347884E-5	0.0	5.939200875770804E-5	5.939200875770804E-5
18-19	4.751360700616643E-5	1.187840175154161E-4	0.0	6.533120963347884E-5	7.127041050924965E-5
20-21	5.345280788193724E-5	1.6629762452158252E-4	0.0	1.6629762452158254E-4	7.127041050924965E-5
22-23	7.127041050924965E-5	2.1381123152774896E-4	0.0	3.741696551735607E-4	7.720961138502045E-5
24-25	8.314881226079126E-5	2.2568963327929055E-4	0.0	5.582848823224556E-4	8.314881226079126E-5
26-27	8.314881226079126E-5	2.2568963327929055E-4	0.0	9.027585331171622E-4	8.314881226079126E-5
28-29	8.314881226079126E-5	2.2568963327929055E-4	0.0	0.003343770093058963	8.314881226079126E-5
30-31	8.908801313656207E-5	2.494464367823738E-4	0.0	0.011070670432436779	8.908801313656207E-5
32-33	9.502721401233286E-5	2.494464367823738E-4	0.0	0.030177079649791456	9.502721401233286E-5
34-35	9.502721401233286E-5	3.2071684729162344E-4	0.0	0.058958447093776775	9.502721401233286E-5
36-37	9.502721401233286E-5	3.5635205254624827E-4	0.0	0.09557955969377956	9.502721401233286E-5
38-39	9.502721401233286E-5	3.6823045429778986E-4	0.0	0.1535699170448057	1.0690561576387448E-4
40-41	1.0690561576387448E-4	4.0980486042818546E-4	0.0	0.24471289368438445	1.0690561576387448E-4
42-43	1.1878401751541609E-4	4.216832621797271E-4	0.0	0.29516640512405745	1.0690561576387448E-4
44-45	1.306624192669577E-4	4.513792665585811E-4	0.0	0.36660905245870445	1.0690561576387448E-4
46-47	1.306624192669577E-4	4.810752709374351E-4	0.0	0.4411935370566342	1.0690561576387448E-4
48-49	1.425408210184993E-4	4.929536726889768E-4	0.0	0.5001341665477836	1.425408210184993E-4
50-51	1.722368253973533E-4	5.404672796951432E-4	0.0	0.55485202421626	1.6035842364581172E-4
52-53	1.7817602627312413E-4	5.46406480570914E-4	0.0	0.6240971672268719	1.6629762452158252E-4
54-55	1.7817602627312413E-4	5.701632840739972E-4	0.0	0.7479295054866932	1.6629762452158252E-4
56-57	1.7817602627312413E-4	5.701632840739972E-4	0.0	0.8733654279829726	1.722368253973533E-4
58-59	1.7817602627312413E-4	5.820416858255389E-4	0.0	0.9750861213822991	1.7817602627312413E-4
60-61	2.0193282977620734E-4	5.998592884528512E-4	0.0	1.0352561654547332	1.7817602627312413E-4
62-63	2.0787203065197814E-4	6.176768910801637E-4	0.0	1.0710754859365068	1.9005442802466573E-4
64-65	2.1381123152774896E-4	6.770688998378717E-4	0.0	1.0970000977592465	1.9005442802466573E-4
66-67	2.1381123152774896E-4	7.245825068440381E-4	0.0	1.1105474149568797	1.9005442802466573E-4
68-69	2.1381123152774896E-4	7.602177120986629E-4	0.0	1.1230078583942467	1.9005442802466573E-4
70-71	2.1381123152774896E-4	7.720961138502045E-4	0.0	1.1341854344424473	2.1975043240351976E-4
72-73	2.2568963327929055E-4	7.780353147259754E-4	0.0	1.1423102612405018	2.494464367823738E-4
74-75	2.2568963327929055E-4	8.017921182290587E-4	0.0	1.1488196254003467	2.613248385339154E-4
76-77	2.2568963327929055E-4	8.314881226079126E-4	0.0	1.155109239127788	2.672640394096862E-4
78-79	2.3162883415506138E-4	8.433665243594542E-4	0.0	1.160448580715106	2.73203240285457E-4
80-81	2.3756803503083217E-4	8.552449261109958E-4	0.0	1.1636260531836433	2.73203240285457E-4
82-83	2.3756803503083217E-4	8.552449261109958E-4	0.0	1.1655147190621384	2.73203240285457E-4
84-85	2.3756803503083217E-4	8.611841269867667E-4	0.0	1.167130181700348	2.850816420369986E-4
86-87	2.3756803503083217E-4	8.671233278625374E-4	0.0	1.1673736889362547	2.850816420369986E-4
88-89	2.3756803503083217E-4	8.79001729614079E-4	0.0	1.1674212025432609	2.9102084291276944E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7505	0.0	42.21203	1
GTATCAA	12765	0.0	31.583582	1
TCAACGC	15240	0.0	25.621178	4
ATCAACG	15400	0.0	25.328405	3
CAACGCA	15705	0.0	24.914698	5
AACGCAG	16455	0.0	23.853735	6
TATCAAC	16755	0.0	23.573761	2
GTACATG	18235	0.0	21.323761	1
TACATGG	18600	0.0	20.70726	2
ACGCAGA	19650	0.0	19.954754	7
ACATGGG	18965	0.0	19.725544	3
CGCAGAG	19965	0.0	19.701418	8
CATGGGG	13540	0.0	17.290718	4
AGAGTAC	21500	0.0	16.333988	10-11
GCAGAGT	24485	0.0	15.797017	9
GAGTACT	14560	0.0	15.531533	12-13
CAGAGTA	23890	0.0	14.614241	10-11
AGTACTT	16740	0.0	13.900115	12-13
GAGTACA	13705	0.0	13.499118	1
ATGGGGG	8220	0.0	13.443973	5
>>END_MODULE