FastQCFastQC Report
Fri 27 May 2016
SRR2139836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961009
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74300.045.2524721
GTATCAA123600.031.3545631
TCAACGC151350.024.7371834
ATCAACG153550.024.4897023
CAACGCA156800.023.8773785
AACGCAG160300.023.4072576
TATCAAC165400.022.834922
ACGCAGA191800.019.4778067
CGCAGAG194600.019.1975488
TACATGG160500.018.953542
GTACATG161000.018.869181
ACATGGG167650.018.1203273
AGAGTAC206050.016.74604810-11
GAGTACT140750.016.37777312-13
GCAGAGT228300.016.237879
GTGGTAT38050.016.076031
CATGGGG126200.016.0696144
CAGAGTA225900.015.44720510-11
TGGTATC36650.014.785882
GTACTTT155950.014.7687414-15
Produced by FastQC (version 0.11.5)