##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6961009
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366802427636568	31.0	31.0	34.0	30.0	34.0
2	31.632929938748823	31.0	31.0	34.0	30.0	34.0
3	31.635371826124633	31.0	31.0	34.0	30.0	34.0
4	35.43345957461052	37.0	35.0	37.0	33.0	37.0
5	35.23204538307593	37.0	35.0	37.0	33.0	37.0
6	35.22836445118804	37.0	35.0	37.0	32.0	37.0
7	35.08671501502153	37.0	35.0	37.0	32.0	37.0
8	35.06562195221986	37.0	35.0	37.0	32.0	37.0
9	36.5514051195739	38.0	35.0	39.0	32.0	39.0
10-11	36.447150765068685	38.0	35.0	39.0	32.0	39.0
12-13	36.377929262841064	38.0	35.0	39.0	32.0	39.0
14-15	37.37504936425165	39.0	36.0	40.0	32.0	41.0
16-17	37.40823456771856	39.0	36.0	40.0	32.0	41.0
18-19	37.42710553886656	39.0	36.0	40.0	32.0	41.0
20-21	37.40230731165721	39.0	36.0	40.0	32.0	41.0
22-23	37.33121145979936	39.0	36.0	40.0	32.0	41.0
24-25	37.225564351664964	39.0	36.0	40.0	31.0	41.0
26-27	37.031306734631656	39.0	36.0	40.0	31.0	41.0
28-29	36.87379073474608	39.0	36.0	40.0	30.5	41.0
30-31	36.71780066505235	38.5	35.5	40.0	30.0	41.0
32-33	36.95406759237319	39.0	36.0	40.0	31.0	41.0
34-35	36.95190879530044	39.0	36.0	40.0	30.5	41.0
36-37	36.83173168287155	38.5	35.0	40.0	30.0	41.0
38-39	36.70361329511405	38.0	35.0	40.0	30.0	41.0
40-41	36.67745665685895	38.0	35.0	40.0	30.0	41.0
42-43	36.59378082171936	38.0	35.0	40.0	30.0	41.0
44-45	36.56611633020083	38.0	35.0	40.0	30.0	41.0
46-47	36.58314507912843	38.0	35.0	40.0	30.0	41.0
48-49	36.49915872095353	38.0	35.0	40.0	30.0	41.0
50-51	36.41451956182951	38.0	35.0	40.0	30.0	41.0
52-53	36.27587449457066	38.0	35.0	40.0	29.5	41.0
54-55	36.15546389537807	38.0	34.0	40.0	29.0	41.0
56-57	35.97191656341269	38.0	34.0	40.0	28.5	41.0
58-59	35.81678311068576	38.0	34.0	40.0	28.0	41.0
60-61	35.65100361340541	38.0	34.0	40.0	28.0	41.0
62-63	35.423679475417394	37.0	34.0	40.0	28.0	41.0
64-65	35.21338672272205	37.0	33.5	39.5	28.0	41.0
66-67	34.96046109316377	36.0	33.0	39.0	28.0	40.5
68-69	34.69193985509082	36.0	33.0	39.0	28.0	40.0
70-71	34.412457211197	35.5	32.5	39.0	27.5	40.0
72-73	34.11278385073341	35.0	32.0	38.0	27.0	40.0
74-75	33.79441524717147	35.0	32.0	37.5	26.0	39.5
76-77	32.792698762207294	34.0	31.0	36.0	26.0	39.0
78-79	33.177000966086986	35.0	31.5	36.0	26.0	39.0
80-81	33.12114493180583	35.0	32.0	36.0	26.0	38.5
82-83	32.94341789588715	34.0	32.0	36.0	26.0	37.0
84-85	32.71087058575211	34.0	31.0	35.0	26.0	37.0
86-87	32.54872670610191	34.0	31.0	35.0	26.0	36.5
88-89	32.363848086448954	34.0	31.0	35.0	26.0	36.0
90-91	32.27631801855165	34.0	31.0	35.0	26.0	36.0
92-93	32.24997062922445	34.0	31.0	35.0	26.0	35.5
94-95	32.25641298170849	34.0	31.0	35.0	26.5	35.0
96-97	32.17203526290047	34.0	31.0	35.0	26.5	35.0
98-99	32.344664328672096	34.0	31.0	35.0	27.0	35.0
100-101	31.89909766194922	33.5	30.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	1.0
19	18.0
20	107.0
21	688.0
22	2804.0
23	9235.0
24	22414.0
25	44499.0
26	75866.0
27	114095.0
28	156141.0
29	199430.0
30	246101.0
31	297054.0
32	358043.0
33	436038.0
34	549075.0
35	705363.0
36	958221.0
37	1261589.0
38	1108642.0
39	409665.0
40	5917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.14506172883845	23.58339143075379	15.397293696933879	24.874253143473883
2	15.038592153194621	24.16334105315269	37.979169560820765	22.818897232831926
3	17.43557579080849	27.36198157479756	31.731965868741156	23.470476765652794
4	12.816733895905033	19.6821179228471	37.86320345225814	29.63794472898972
5	12.616102062215406	38.40680280689193	36.21170436642159	12.765390764471071
6	30.117573472466418	39.31618246722566	17.839310364345167	12.726933695962755
7	25.65224380545981	32.886755354001124	23.91489509638617	17.546105744152896
8	24.29881932346302	37.412866439333726	21.209453974272982	17.078860262930274
9	25.26725651410593	15.631900490288118	21.11168079225296	37.98916220335299
10-11	24.003804620853096	27.04694103972571	29.54097602804421	19.408278311376986
12-13	24.463434536140234	24.69754803230598	29.49689266104865	21.342124770505134
14-15	21.55762189073452	26.225788531518923	26.730887720443974	25.485701857302583
16-17	21.418971014115915	29.154566529076458	27.622022899266476	21.804439557541155
18-19	21.47214648055586	28.434529714464713	29.10859443108465	20.984729373894776
20-21	22.098051058256775	27.737925678224773	28.938562462593016	21.22546080092544
22-23	21.831310783454622	27.730851848996707	28.80484569955229	21.632991667996386
24-25	21.691519435162242	27.836087644195562	28.85646698862116	21.61592593202104
26-27	21.75327752079956	27.922504279122297	28.765846916781477	21.558371283296662
28-29	21.686943735450438	27.972204397457407	28.814834584583863	21.526017282508295
30-31	21.85413165798797	27.889635068380734	28.74623836789828	21.50999490573301
32-33	21.72100352032704	28.022256768886166	28.68661766599436	21.570122044792438
34-35	21.80371229338442	28.00621917262648	28.723078275875892	21.46699025811321
36-37	21.761821318009655	28.020421782223266	28.6699956308621	21.54776126890498
38-39	21.701839478384795	28.04945379293098	28.728582403604975	21.520124325079255
40-41	21.867287334346504	27.957525999197134	28.59509957185808	21.580087094598284
42-43	21.782381294226234	28.109608792808373	28.37620329601095	21.731806616954447
44-45	21.877566356932547	28.168846963003396	28.205689281202673	21.747897398861383
46-47	21.931829016513074	28.114239272735276	28.04098840087571	21.91294330987594
48-49	21.83742378599738	28.26371040283205	28.04625044308781	21.852615368082756
50-51	21.903888368599862	28.338081855512304	27.9183244284049	21.839705347482937
52-53	22.008630014599746	28.222038355462498	27.816614519459783	21.952717110477977
54-55	21.95246964006658	28.132185698949385	27.934554165172752	21.980790495811284
56-57	21.96937423453145	28.168709813279563	27.907767331628808	21.954148620560183
58-59	21.9454841527215	28.195188989959156	27.886177068243267	21.97314978907608
60-61	21.988010190469453	28.128632547042404	27.95794756102555	21.925409701462595
62-63	21.971890102221238	28.22631513027626	27.87144159884521	21.930353168657295
64-65	21.889169223165922	28.214510710142203	27.831324340158435	22.06499572653344
66-67	21.921442836715393	28.299819428507885	27.72152414079636	22.057213593980364
68-69	21.91367300158005	28.30208201250985	27.704177718618478	22.08006726729162
70-71	21.920860287740105	28.083178213466475	27.79868133615987	22.19728016263355
72-73	21.93393124650537	28.156375895127795	27.708910025205956	22.20078283316088
74-75	21.905152494955892	28.108487646974815	27.72107099831192	22.26528885975737
76-77	22.033524588394744	28.022752256841976	27.71525311834567	22.2284700364176
78-79	22.020209303274008	28.0770296001221	27.618176280179757	22.284584816424136
80-81	22.019961673602097	28.084862209653355	27.579823139020814	22.31535297772373
82-83	22.00287111653431	28.038219813568727	27.70498939150596	22.253919678391
84-85	22.025405346280575	28.10092214624381	27.65524726335384	22.21842524412177
86-87	22.032286523889663	28.03463733315988	27.7198218906211	22.213254252329357
88-89	21.969525957021887	28.02591150039265	27.747159895188318	22.257402647397146
90-91	21.96103178901774	27.987859731096	27.853117744738874	22.197990735147393
92-93	21.992795124943566	27.873177393871874	27.892812408981253	22.241215072203303
94-95	21.83170997425901	27.90495314441995	28.004828364696756	22.258508516624286
96-97	21.726559010047765	27.995254808077984	28.08333581250472	22.194850369369526
98-99	21.896589778893777	28.64070965958407	28.13997650078791	21.322724060734245
100-101	25.707621390122792	35.826013349188116	28.96709391326344	9.499271347425642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1265.0
1	1147.0
2	1546.0
3	2183.5
4	2770.0
5	3973.0
6	5112.5
7	6367.5
8	7418.5
9	7757.5
10	7592.5
11	7376.0
12	8302.5
13	11150.0
14	18008.5
15	27358.0
16	34324.5
17	37312.0
18	38087.0
19	37987.5
20	37253.5
21	36976.5
22	38121.0
23	42187.5
24	50030.0
25	61207.0
26	74823.5
27	89613.5
28	105711.5
29	122352.5
30	138080.0
31	154286.0
32	170883.5
33	186748.0
34	202013.5
35	216118.0
36	230621.0
37	243933.0
38	254917.0
39	264376.0
40	273422.0
41	281530.0
42	287451.0
43	293417.5
44	298506.0
45	301431.0
46	301078.0
47	298995.0
48	295989.5
49	289562.5
50	282196.0
51	272816.5
52	259242.5
53	244496.5
54	228560.5
55	210867.0
56	192629.5
57	174042.5
58	154423.5
59	133284.0
60	111967.0
61	91821.5
62	73564.5
63	57599.5
64	43458.5
65	32494.5
66	24410.0
67	18624.5
68	14386.5
69	10646.0
70	7695.0
71	5586.5
72	4129.0
73	3019.5
74	2159.5
75	1475.5
76	1017.5
77	710.5
78	505.0
79	366.5
80	260.0
81	180.5
82	136.5
83	97.5
84	69.5
85	50.0
86	34.0
87	26.0
88	18.5
89	14.0
90	10.5
91	8.0
92	7.5
93	4.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001982471219330416
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.91235609665208E-4
14-15	0.0
16-17	0.0
18-19	4.3815487093896874E-4
20-21	0.0
22-23	0.0014799714954616243
24-25	1.5091369619405718E-4
26-27	0.00476673586222451
28-29	4.0285404828033114E-4
30-31	0.0
32-33	0.0
34-35	2.1022429482435036E-4
36-37	0.017700916335904485
38-39	0.0076106804881325915
40-41	0.013223798424294827
42-43	0.0015610607803869818
44-45	0.0029134850428772915
46-47	0.0
48-49	5.643660221348992E-4
50-51	2.3450130093505048E-5
52-53	0.0
54-55	0.003790334344195554
56-57	0.0073297158871349685
58-59	0.010457268577786965
60-61	0.005261241747357653
62-63	0.005972986018856541
64-65	5.770334574181555E-4
66-67	5.164347171861469E-5
68-69	0.0
70-71	5.3254932604995205E-5
72-73	0.0
74-75	1.0069893298495164E-4
76-77	9.303494792694412E-6
78-79	0.0
80-81	0.0018304465104512233
82-83	0.001844324312658098
84-85	0.00861987337276168
86-87	0.009954371724943
88-89	0.04419243467903141
90-91	4.2488423763393016E-4
92-93	5.877409003015111E-4
94-95	0.0
96-97	0.008287655733558814
98-99	2.4442694347529164E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	248.0
20-21	828.0
22-23	1612.0
24-25	3000.0
26-27	3757.0
28-29	6735.0
30-31	16009.0
32-33	23576.0
34-35	34966.0
36-37	50223.0
38-39	53111.0
40-41	57174.0
42-43	66432.0
44-45	78551.0
46-47	78863.0
48-49	72626.0
50-51	66529.0
52-53	63431.0
54-55	70784.0
56-57	73633.0
58-59	74899.0
60-61	77607.0
62-63	74526.0
64-65	81284.0
66-67	86936.0
68-69	88366.0
70-71	87253.0
72-73	84891.0
74-75	87171.0
76-77	88460.0
78-79	93964.0
80-81	93926.0
82-83	91000.0
84-85	92176.0
86-87	97859.0
88-89	104084.0
90-91	112058.0
92-93	118022.0
94-95	144650.0
96-97	181619.0
98-99	550842.0
100-101	3627328.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.73942199480042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.98168588814278	81.62401639821081
2	5.854562591486451	10.390610008017772
3	1.2812433011351405	3.410903699323272
4	0.4393047480658229	1.5593459769173017
5	0.17682030064698215	0.7845465638180017
6	0.09949791378019104	0.5297632415125586
7	0.04494833071157019	0.27920822208831125
8	0.03205345049679479	0.22755237360196143
9	0.01783522554345673	0.14244188452859255
>10	0.07091446304843338	0.9708819011929805
>50	9.65408110512955E-4	0.05260862686894695
>100	1.6837883181257702E-4	0.02812110391942003
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.4365733473408812E-5	0.0
3	0.0	0.0	0.0	1.4365733473408812E-5	0.0
4	0.0	5.746293389363525E-5	0.0	1.4365733473408812E-5	0.0
5	1.4365733473408812E-5	7.182866736704406E-5	0.0	1.4365733473408812E-5	0.0
6	1.4365733473408812E-5	7.182866736704406E-5	0.0	1.4365733473408812E-5	0.0
7	2.8731466946817624E-5	7.182866736704406E-5	0.0	2.8731466946817624E-5	0.0
8	2.8731466946817624E-5	8.619440084045287E-5	0.0	4.309720042022643E-5	0.0
9	2.8731466946817624E-5	1.292916012606793E-4	0.0	1.0056013431386168E-4	0.0
10-11	4.309720042022643E-5	1.292916012606793E-4	0.0	1.0056013431386168E-4	0.0
12-13	4.309720042022643E-5	1.292916012606793E-4	0.0	1.0056013431386168E-4	0.0
14-15	5.746293389363525E-5	1.5802306820749694E-4	0.0	1.0056013431386168E-4	0.0
16-17	7.182866736704406E-5	1.5802306820749694E-4	0.0	1.0774300105056609E-4	0.0
18-19	7.182866736704406E-5	1.6520593494420135E-4	0.0	1.3647446799738372E-4	0.0
20-21	8.619440084045287E-5	1.7238880168090573E-4	0.0	2.585832025213586E-4	7.182866736704406E-6
22-23	1.149258677872705E-4	2.0112026862772336E-4	0.0	4.59703471149082E-4	1.4365733473408812E-5
24-25	1.292916012606793E-4	2.1548600210113218E-4	0.0	0.0010558814102955478	2.8731466946817624E-5
26-27	1.5802306820749692E-4	2.29851735574541E-4	0.0	0.00314609563067653	4.309720042022643E-5
28-29	1.7238880168090573E-4	2.29851735574541E-4	0.0	0.015249226082023455	4.309720042022643E-5
30-31	1.9393740189101896E-4	2.442174690479498E-4	0.0	0.023610082963547382	4.309720042022643E-5
32-33	2.29851735574541E-4	2.5140033578465424E-4	0.0	0.03050563503078361	5.746293389363525E-5
34-35	3.0168040294158507E-4	3.0886326967828945E-4	0.0	0.041021351933318864	5.746293389363525E-5
36-37	3.160461364149939E-4	3.6632620357192466E-4	0.0	0.05561693714230222	5.746293389363525E-5
38-39	3.4477760336181146E-4	3.735090703086291E-4	0.0	0.08159736612896205	5.746293389363525E-5
40-41	3.4477760336181146E-4	4.237891374655599E-4	0.0	0.130756906074967	5.746293389363525E-5
42-43	3.4477760336181146E-4	4.3097200420226436E-4	0.0	0.15330392476148214	5.746293389363525E-5
44-45	3.591433368352203E-4	4.884349380958996E-4	0.0	0.18641694041768947	5.746293389363525E-5
46-47	3.735090703086291E-4	5.31532138516126E-4	0.0	0.22476626592495427	7.182866736704406E-5
48-49	3.735090703086291E-4	5.31532138516126E-4	0.0	0.25651453690118775	7.182866736704406E-5
50-51	3.735090703086291E-4	6.033608058831701E-4	0.0	0.28613667932335674	7.182866736704406E-5
52-53	3.735090703086291E-4	6.033608058831701E-4	0.0	0.326346367315428	7.182866736704406E-5
54-55	3.735090703086291E-4	6.464580063033965E-4	0.0	0.40992620466372043	7.182866736704406E-5
56-57	3.735090703086291E-4	6.608237397768053E-4	0.0	0.4971046582471018	7.182866736704406E-5
58-59	3.735090703086291E-4	6.608237397768053E-4	0.0	0.5852025187727814	7.182866736704406E-5
60-61	3.8069193704533353E-4	6.895552067236229E-4	0.0	0.6407907244481368	7.182866736704406E-5
62-63	3.878748037820379E-4	6.895552067236229E-4	0.0	0.6664838387653285	8.619440084045287E-5
64-65	3.878748037820379E-4	7.97298207774189E-4	0.0	0.6922703303500972	8.619440084045287E-5
66-67	3.878748037820379E-4	8.188468079843023E-4	0.0	0.7039424887972419	8.619440084045287E-5
68-69	3.878748037820379E-4	8.475782749311198E-4	0.0	0.7139913193618914	8.619440084045287E-5
70-71	3.878748037820379E-4	8.763097418779375E-4	0.0	0.7230920115172959	8.619440084045287E-5
72-73	4.0942340399215116E-4	8.834926086146419E-4	0.0	0.7300450265184257	1.0774300105056609E-4
74-75	4.1660627072885554E-4	9.409555425082772E-4	0.0	0.7360930003107309	1.149258677872705E-4
76-77	4.1660627072885554E-4	9.768698761917992E-4	0.0	0.7417028192320969	1.221087345239749E-4
78-79	4.1660627072885554E-4	9.768698761917992E-4	0.0	0.7464506941450586	1.292916012606793E-4
80-81	4.1660627072885554E-4	9.768698761917992E-4	0.0	0.7493022922395303	1.3647446799738372E-4
82-83	4.1660627072885554E-4	9.768698761917992E-4	0.0	0.7511482889908632	1.4365733473408813E-4
84-85	4.1660627072885554E-4	9.91235609665208E-4	0.0	0.7528362626739888	1.4365733473408813E-4
86-87	4.1660627072885554E-4	0.0010199670766120256	0.0	0.7530804801430367	1.4365733473408813E-4
88-89	4.1660627072885554E-4	0.001063064277032252	0.0	0.7531379430769304	1.4365733473408813E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7430	0.0	45.252472	1
GTATCAA	12360	0.0	31.354563	1
TCAACGC	15135	0.0	24.737183	4
ATCAACG	15355	0.0	24.489702	3
CAACGCA	15680	0.0	23.877378	5
AACGCAG	16030	0.0	23.407257	6
TATCAAC	16540	0.0	22.83492	2
ACGCAGA	19180	0.0	19.477806	7
CGCAGAG	19460	0.0	19.197548	8
TACATGG	16050	0.0	18.95354	2
GTACATG	16100	0.0	18.86918	1
ACATGGG	16765	0.0	18.120327	3
AGAGTAC	20605	0.0	16.746048	10-11
GAGTACT	14075	0.0	16.377773	12-13
GCAGAGT	22830	0.0	16.23787	9
GTGGTAT	3805	0.0	16.07603	1
CATGGGG	12620	0.0	16.069614	4
CAGAGTA	22590	0.0	15.447205	10-11
TGGTATC	3665	0.0	14.78588	2
GTACTTT	15595	0.0	14.76874	14-15
>>END_MODULE