FastQCFastQC Report
Fri 27 May 2016
SRR2139835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8806302
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82800.043.5867161
GTATCAA142950.031.7294181
TCAACGC173950.025.5114
ATCAACG177250.025.0590723
CAACGCA180700.024.5806355
TATCAAC189350.023.716992
AACGCAG187600.023.654796
GTACATG200100.020.0965421
ACGCAGA224700.019.7675637
CGCAGAG227000.019.6032438
TACATGG206900.019.3965852
ACATGGG209250.018.5930143
AGAGTAC242850.016.4830210-11
CATGGGG153050.016.24454
GCAGAGT274300.015.9252139
GAGTACT164450.015.49094812-13
TATACTG50550.015.102295
CAGAGTA267700.014.82331110-11
TATACCG6250.014.3702745
AGTACTT186650.014.32651712-13
Produced by FastQC (version 0.11.5)