##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8806302
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.490563235283098	31.0	31.0	34.0	30.0	34.0
2	31.744116202237898	31.0	31.0	34.0	30.0	34.0
3	31.755297967296602	31.0	31.0	34.0	30.0	34.0
4	35.53096214506384	37.0	35.0	37.0	33.0	37.0
5	35.34736714684552	37.0	35.0	37.0	33.0	37.0
6	35.335700728864396	37.0	35.0	37.0	33.0	37.0
7	35.197548641870334	37.0	35.0	37.0	32.0	37.0
8	35.1754709297955	37.0	35.0	37.0	32.0	37.0
9	36.67254552478441	39.0	35.0	39.0	32.0	39.0
10-11	36.587053680421135	38.0	35.0	39.0	32.0	39.0
12-13	36.51987940000241	38.0	35.0	39.0	32.0	39.0
14-15	37.553467051209466	39.0	36.0	41.0	32.0	41.0
16-17	37.586511171204435	39.0	36.0	41.0	32.0	41.0
18-19	37.60731683969048	39.0	36.5	41.0	32.0	41.0
20-21	37.58106223453577	39.0	36.0	41.0	32.0	41.0
22-23	37.511088568075415	39.0	36.0	41.0	32.0	41.0
24-25	37.410174395523505	39.0	36.0	41.0	32.0	41.0
26-27	37.2268668625989	39.0	36.0	40.0	31.0	41.0
28-29	37.07472146482776	39.0	36.0	40.0	31.0	41.0
30-31	36.92567937023091	39.0	36.0	40.0	31.0	41.0
32-33	37.16333830828539	39.0	36.0	40.0	31.0	41.0
34-35	37.168672481236044	39.0	36.0	40.0	31.0	41.0
36-37	37.05134261452699	39.0	36.0	40.0	31.0	41.0
38-39	36.93326007117727	39.0	35.0	40.0	30.0	41.0
40-41	36.91256411939094	39.0	35.0	40.0	30.0	41.0
42-43	36.83993877965213	39.0	35.0	40.0	30.0	41.0
44-45	36.81723652649691	39.0	35.0	40.0	30.0	41.0
46-47	36.836363318433904	39.0	35.0	40.0	30.0	41.0
48-49	36.760100966065586	39.0	35.0	40.0	30.0	41.0
50-51	36.67588092133506	39.0	35.0	40.0	30.0	41.0
52-53	36.549509470885795	38.5	35.0	40.0	30.0	41.0
54-55	36.44274816139573	38.0	35.0	40.0	30.0	41.0
56-57	36.268628932305134	38.0	34.5	40.0	29.5	41.0
58-59	36.12385880998815	38.0	34.0	40.0	29.0	41.0
60-61	35.96621733308941	38.0	34.0	40.0	29.0	41.0
62-63	35.738240167281305	37.5	34.0	40.0	28.0	41.0
64-65	35.5285667736441	37.0	34.0	40.0	28.0	41.0
66-67	35.275710045370516	37.0	34.0	39.0	28.0	41.0
68-69	35.00241808524952	36.0	33.0	39.0	28.0	40.5
70-71	34.722949466521584	36.0	33.0	39.0	28.0	40.0
72-73	34.419377941964754	35.0	33.0	38.5	28.0	40.0
74-75	34.10110234678163	35.0	32.5	37.5	28.0	40.0
76-77	33.07675999833373	34.0	31.0	36.0	26.0	39.0
78-79	33.45154427852647	35.0	32.0	36.5	27.0	39.0
80-81	33.38416250282721	35.0	32.0	36.0	27.0	38.5
82-83	33.19412234367296	35.0	32.0	36.0	27.0	37.5
84-85	32.9645305828382	34.0	32.0	35.5	27.0	37.0
86-87	32.79276488757223	34.0	32.0	35.0	27.0	37.0
88-89	32.60131968095682	34.0	32.0	35.0	27.0	36.0
90-91	32.504011776499894	34.0	32.0	35.0	27.0	36.0
92-93	32.46523048930669	34.0	32.0	35.0	27.0	36.0
94-95	32.46002686104766	34.0	32.0	35.0	27.0	35.0
96-97	32.36788439356675	34.0	31.0	35.0	27.0	35.0
98-99	32.521230937375776	34.0	31.5	35.0	27.5	35.0
100-101	32.0723824109449	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	20.0
20	155.0
21	698.0
22	3037.0
23	9483.0
24	23840.0
25	48360.0
26	83331.0
27	127021.0
28	177045.0
29	230529.0
30	288204.0
31	351183.0
32	427109.0
33	525578.0
34	662271.0
35	855064.0
36	1168091.0
37	1604790.0
38	1550923.0
39	654981.0
40	14587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.938444763761225	23.08881753090003	14.94840853743149	25.024329167907254
2	14.762682957212903	23.82509420470313	38.601006644379815	22.81121619370415
3	17.234839323021173	27.41295949196382	31.72196456583024	23.63023661918476
4	12.469558731917212	19.447686440914698	38.4156255372573	29.667129289910793
5	12.52084018921904	38.42174615406104	36.376551701270294	12.680861955449632
6	29.79795605465268	39.29539323089306	18.204928697653113	12.70172201680115
7	25.428800874646363	32.93791196350068	24.196649172376784	17.43663798947617
8	24.10088820483331	37.3920744485029	21.498490512816844	17.008546833846943
9	25.252699714363647	15.734595520344408	21.355626913544416	37.65707785174753
10-11	23.827197840818997	27.038432249995516	29.79712710284067	19.337242806344822
12-13	24.304577554753852	24.72128254656079	29.695499663306546	21.278640235378816
14-15	21.43725027826663	26.26147729205744	26.957632159333166	25.34364027034276
16-17	21.328708690662666	29.104117710248868	27.827094732840184	21.740078866248286
18-19	21.33475096173096	28.53000429126699	29.229512606774932	20.905732140227112
20-21	22.002549224285403	27.799428241516743	29.02829221051178	21.16973032368607
22-23	21.713939494989717	27.795634820127084	28.857944590569318	21.63248109431388
24-25	21.563657191169963	27.870882139774906	28.95065693822067	21.61480373083446
26-27	21.618340492822625	27.97706273313049	28.851432792037144	21.55316398200974
28-29	21.608960022420757	28.06632458481733	28.84277637490072	21.481939017861194
30-31	21.787405260153776	27.986326274695806	28.761205639756724	21.46506282539369
32-33	21.622157365471974	28.139792471724633	28.674446621247906	21.56360354155548
34-35	21.719720798532116	28.119648498515954	28.728051299522257	21.432579403429674
36-37	21.71144712685763	28.089935425309783	28.700961277402264	21.497656170430325
38-39	21.639843365893785	28.161135163849536	28.70573241989618	21.4932890503605
40-41	21.824490021118766	28.044101712862258	28.59181615521061	21.53959211080837
42-43	21.6829373574357	28.215375515441288	28.375323607847896	21.726363519275115
44-45	21.832636608434616	28.220884109418193	28.20579873777736	21.740680544369834
46-47	21.853120543705817	28.201486695591495	28.056992020388964	21.888400740313724
48-49	21.779564337185334	28.31159382217507	28.06834357860221	21.840498262037382
50-51	21.82166738111041	28.344395708064734	27.999964184543618	21.833972726281246
52-53	21.951978620277536	28.26663361924135	27.88983261001221	21.8915551504689
54-55	21.869996757206785	28.2088335926656	27.96523919083243	21.955930459295185
56-57	21.923033713767463	28.281212948729856	27.892384693627427	21.90336864387525
58-59	21.91483505409301	28.31029742548846	27.888269166705705	21.88659835371282
60-61	21.98791921473174	28.262784826690517	27.902787650405536	21.846508308172204
62-63	21.9692606947225	28.31451652574726	27.879178263797083	21.83704451573316
64-65	21.905186153436528	28.24285315459575	27.901820371718593	21.95014032024913
66-67	21.9826795678655	28.30414739548366	27.749599626294874	21.963573410355966
68-69	21.9606623397562	28.265563995605593	27.78759241408686	21.98618125055135
70-71	21.928524868956753	28.08226784906256	27.872906205585046	22.116301076395644
72-73	21.964701654058565	28.145383308710116	27.79910343994718	22.09081159728414
74-75	21.836386031893397	28.20430519004049	27.819339788596615	22.139968989469494
76-77	21.94601806713176	28.13892657944454	27.798259692867568	22.116795660556132
78-79	21.930794563558052	28.107897892612645	27.781957987099375	22.179349556729928
80-81	21.973235318950344	28.09777346034503	27.772218619207074	22.156772601497554
82-83	21.93367731320268	28.10132277498652	27.875330079571853	22.089669832238947
84-85	21.95047178036308	28.088238300492364	27.834492257382554	22.126797661761998
86-87	21.946343479489606	28.05307660013101	27.832249453976377	22.168330466403006
88-89	21.92234042464505	28.03500999964153	27.839493873916982	22.203155701796433
90-91	21.912922699480987	28.00275269845967	27.962385580003257	22.121939022056086
92-93	21.9989173585118	27.910959934849583	27.92697709659282	22.163145610045795
94-95	21.78087550834361	27.995066928047013	28.04787010329347	22.17618746031591
96-97	21.714779117476592	28.053895839054544	28.07840606025166	22.152918983217205
98-99	21.899157955251127	28.642855534580818	28.149368127725605	21.308618382442457
100-101	25.640974489049302	35.8568571641051	28.99895443094137	9.503213915904228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2484.0
1	2184.5
2	2586.5
3	3444.0
4	4399.0
5	6361.5
6	8094.0
7	9745.0
8	10990.5
9	11126.5
10	10640.0
11	10089.5
12	11075.5
13	14717.5
14	23010.5
15	34102.0
16	42320.5
17	45634.0
18	46409.5
19	46740.0
20	46542.0
21	46846.0
22	48953.0
23	54933.0
24	65318.5
25	79785.5
26	98052.0
27	117224.0
28	137702.5
29	159714.0
30	179823.0
31	199948.5
32	220907.0
33	240261.0
34	258946.0
35	277087.5
36	296536.0
37	313841.0
38	328358.5
39	340227.5
40	350915.5
41	363348.5
42	371412.5
43	376956.5
44	381606.5
45	384298.0
46	385136.5
47	383129.0
48	378565.5
49	370283.5
50	360326.0
51	347607.0
52	329472.0
53	309094.5
54	288181.0
55	265496.0
56	242061.0
57	217832.0
58	192409.0
59	165616.0
60	138907.0
61	113910.5
62	91216.5
63	71444.0
64	53972.0
65	40531.5
66	30426.0
67	23036.0
68	17664.5
69	13041.0
70	9492.0
71	7007.5
72	5024.5
73	3536.5
74	2518.0
75	1805.0
76	1321.0
77	930.0
78	645.5
79	467.0
80	342.5
81	239.0
82	170.5
83	116.0
84	74.5
85	50.5
86	37.5
87	29.5
88	21.0
89	13.0
90	12.0
91	12.5
92	10.0
93	6.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002418722410382928
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.175963077350742E-4
14-15	0.0
16-17	0.0
18-19	5.109976923344215E-4
20-21	0.0
22-23	0.0013629217589777361
24-25	2.158556543701085E-4
26-27	0.005040865965078904
28-29	4.2652942504231594E-4
30-31	0.0
32-33	0.0
34-35	1.5483006139183967E-4
36-37	0.018779423982276378
38-39	0.00766135708055408
40-41	0.012964232041794945
42-43	0.0017417556897352533
44-45	0.003007398259627014
46-47	0.0
48-49	7.066712221356464E-4
50-51	6.217960791155921E-6
52-53	0.0
54-55	0.004625076771821106
56-57	0.009016372417556907
58-59	0.012346315320581875
60-61	0.006376792131872856
62-63	0.007200535242023209
64-65	8.773126539632702E-4
66-67	8.280018066999422E-5
68-69	0.0
70-71	1.2814556424841272E-4
72-73	0.0
74-75	1.1017466724496126E-4
76-77	3.732352690011089E-5
78-79	7.592710603000168E-6
80-81	0.0021107347096406067
82-83	0.002181060286632266
84-85	0.00909045070598893
86-87	0.010257820853365757
88-89	0.043081179274369615
90-91	4.8552744891101305E-4
92-93	6.28120044558138E-4
94-95	8.954242923237962E-6
96-97	0.00853019866111038
98-99	1.4620672500250014E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	308.0
20-21	985.0
22-23	1942.0
24-25	3766.0
26-27	5394.0
28-29	9923.0
30-31	21675.0
32-33	32564.0
34-35	47415.0
36-37	68322.0
38-39	75410.0
40-41	77996.0
42-43	89034.0
44-45	105034.0
46-47	105005.0
48-49	97497.0
50-51	89691.0
52-53	90742.0
54-55	99490.0
56-57	104049.0
58-59	103376.0
60-61	101531.0
62-63	97828.0
64-65	103656.0
66-67	109879.0
68-69	112377.0
70-71	110683.0
72-73	106630.0
74-75	108750.0
76-77	110691.0
78-79	117968.0
80-81	118224.0
82-83	112935.0
84-85	114075.0
86-87	119565.0
88-89	128443.0
90-91	137365.0
92-93	142550.0
94-95	171752.0
96-97	216067.0
98-99	680050.0
100-101	4555665.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.06457307657368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.88805790715081	80.92082590428717
2	5.796939995478685	10.210100917046908
3	1.3166789679988506	3.478583135871672
4	0.4657973468183435	1.6408097795103258
5	0.19724613375585756	0.8685198280107174
6	0.1040914840277069	0.5500063261084198
7	0.06739489453189491	0.41545718301444257
8	0.0417909386571523	0.29442409370491174
9	0.027389138762000442	0.21708115308095438
>10	0.09270287206976469	1.2705853163100398
>50	0.001679889343583359	0.09545571043597528
>100	2.3043140535347164E-4	0.0381506526184364
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2711008548196507E-5
2	0.0	0.0	0.0	0.0	2.2711008548196507E-5
3	0.0	2.2711008548196507E-5	0.0	0.0	2.2711008548196507E-5
4	0.0	6.813302564458953E-5	0.0	0.0	2.2711008548196507E-5
5	0.0	7.948852991868777E-5	0.0	0.0	2.2711008548196507E-5
6	0.0	7.948852991868777E-5	0.0	0.0	2.2711008548196507E-5
7	1.1355504274098253E-5	7.948852991868777E-5	0.0	0.0	2.2711008548196507E-5
8	1.1355504274098253E-5	7.948852991868777E-5	0.0	0.0	2.2711008548196507E-5
9	1.1355504274098253E-5	1.5897705983737554E-4	0.0	0.0	3.406651282229476E-5
10-11	2.2711008548196507E-5	1.6465481197442468E-4	0.0	2.8388760685245635E-5	3.406651282229476E-5
12-13	2.2711008548196507E-5	1.703325641114738E-4	0.0	4.542201709639301E-5	3.406651282229476E-5
14-15	2.2711008548196507E-5	2.5549884616721073E-4	0.0	6.24552735075404E-5	5.677752137049127E-5
16-17	2.2711008548196507E-5	2.725321025783581E-4	0.0	6.813302564458953E-5	5.677752137049127E-5
18-19	2.2711008548196507E-5	2.725321025783581E-4	0.0	7.381077778163865E-5	5.677752137049127E-5
20-21	2.2711008548196507E-5	2.725321025783581E-4	0.0	9.084403419278603E-5	5.677752137049127E-5
22-23	3.406651282229476E-5	3.12276367537702E-4	0.0	9.652178632983515E-5	5.677752137049127E-5
24-25	4.542201709639301E-5	3.2930962394884936E-4	0.0	1.4194380342622817E-4	5.677752137049127E-5
26-27	4.542201709639301E-5	3.406651282229476E-4	0.0	2.3278783761901422E-4	5.677752137049127E-5
28-29	4.542201709639301E-5	3.4634288035999673E-4	0.0	5.8480847011606E-4	5.677752137049127E-5
30-31	5.109976923344214E-5	3.974426495934389E-4	0.0	0.0015897705983737554	5.677752137049127E-5
32-33	5.677752137049127E-5	4.0879815386753713E-4	0.0	0.0038438381967822585	5.677752137049127E-5
34-35	7.948852991868777E-5	4.655756752380284E-4	0.0	0.007517343829453044	5.677752137049127E-5
36-37	1.0219953846688428E-4	5.337087008826179E-4	0.0	0.012916886111786763	5.677752137049127E-5
38-39	1.0219953846688428E-4	5.564197094308144E-4	0.0	0.021217759736152586	5.677752137049127E-5
40-41	1.1355504274098254E-4	5.904862222531092E-4	0.0	0.029700321428903984	5.677752137049127E-5
42-43	1.1355504274098254E-4	6.415859914865514E-4	0.0	0.036553368258322276	5.677752137049127E-5
44-45	1.1355504274098254E-4	7.551410342275339E-4	0.0	0.044973474677566135	6.24552735075404E-5
46-47	1.1355504274098254E-4	8.289518120091725E-4	0.0	0.05461429780627555	7.381077778163865E-5
48-49	1.1355504274098254E-4	8.346295641462216E-4	0.0	0.06350565765289448	7.948852991868777E-5
50-51	1.1355504274098254E-4	8.800515812426147E-4	0.0	0.07161348770460063	7.948852991868777E-5
52-53	1.1355504274098254E-4	8.97084837653762E-4	0.0	0.07974402876485498	7.948852991868777E-5
54-55	1.1355504274098254E-4	9.027625897908111E-4	0.0	0.08962899523545752	7.948852991868777E-5
56-57	1.1355504274098254E-4	9.084403419278603E-4	0.0	0.0994969284496489	7.948852991868777E-5
58-59	1.1355504274098254E-4	9.084403419278603E-4	0.0	0.10972823780061142	7.948852991868777E-5
60-61	1.1355504274098254E-4	9.425068547501551E-4	0.0	0.11917601735666117	7.948852991868777E-5
62-63	1.1355504274098254E-4	9.822511197094988E-4	0.0	0.1276188347844532	7.948852991868777E-5
64-65	1.1355504274098254E-4	0.0011355504274098253	0.0	0.1361808850071233	8.51662820557369E-5
66-67	1.1355504274098254E-4	0.0011809724445062185	0.0	0.14519147764862028	9.084403419278603E-5
68-69	1.1355504274098254E-4	0.0012036834530544148	0.0	0.1542304590508025	9.084403419278603E-5
70-71	1.1355504274098254E-4	0.0012150389573285132	0.0	0.1625767546922647	9.084403419278603E-5
72-73	1.1355504274098254E-4	0.0012150389573285132	0.0	0.16927650221398266	1.3626605128917905E-4
74-75	1.1355504274098254E-4	0.0012774942308360535	0.0	0.1748179882997426	1.3626605128917905E-4
76-77	1.1355504274098254E-4	0.0013229162479324466	0.0	0.18003584251369076	1.3626605128917905E-4
78-79	1.1355504274098254E-4	0.0013285940000694956	0.0	0.18469159926607104	1.3626605128917905E-4
80-81	1.1355504274098254E-4	0.0013285940000694956	0.0	0.18769513014657002	1.3626605128917905E-4
82-83	1.1355504274098254E-4	0.0013399495043435938	0.0	0.1895290440868369	1.3626605128917905E-4
84-85	1.1355504274098254E-4	0.0013513050086176922	0.0	0.1910336484031549	1.3626605128917905E-4
86-87	1.1355504274098254E-4	0.0013626605128917904	0.0	0.19127779174504805	1.3626605128917905E-4
88-89	1.1355504274098254E-4	0.001385371521439987	0.0	0.19130618050573328	1.3626605128917905E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8280	0.0	43.586716	1
GTATCAA	14295	0.0	31.729418	1
TCAACGC	17395	0.0	25.511	4
ATCAACG	17725	0.0	25.059072	3
CAACGCA	18070	0.0	24.580635	5
TATCAAC	18935	0.0	23.71699	2
AACGCAG	18760	0.0	23.65479	6
GTACATG	20010	0.0	20.096542	1
ACGCAGA	22470	0.0	19.767563	7
CGCAGAG	22700	0.0	19.603243	8
TACATGG	20690	0.0	19.396585	2
ACATGGG	20925	0.0	18.593014	3
AGAGTAC	24285	0.0	16.48302	10-11
CATGGGG	15305	0.0	16.2445	4
GCAGAGT	27430	0.0	15.925213	9
GAGTACT	16445	0.0	15.490948	12-13
TATACTG	5055	0.0	15.10229	5
CAGAGTA	26770	0.0	14.823311	10-11
TATACCG	625	0.0	14.370274	5
AGTACTT	18665	0.0	14.326517	12-13
>>END_MODULE