FastQCFastQC Report
Fri 27 May 2016
SRR2139834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10179204
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93850.044.1817931
GTATCAA161250.030.380531
TCAACGC189850.024.8456084
ATCAACG193600.024.4070593
CAACGCA197800.023.867915
AACGCAG205550.022.9881116
TATCAAC210150.022.957162
GTACATG235450.020.5605581
TACATGG235800.020.0216082
ACGCAGA243750.019.4367267
ACATGGG238050.019.2591863
CGCAGAG247550.019.1383658
AGAGTAC263350.016.43601210-11
CATGGGG178250.016.3970014
GAGTACT175050.015.54008112-13
GCAGAGT304550.015.2306129
CAGAGTA294250.014.724071510-11
GTGGTAT54400.013.9828481
AGTACTT198600.013.86387112-13
GAGTACA173200.013.8199791
Produced by FastQC (version 0.11.5)