##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10179204
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557435237568676	31.0	31.0	34.0	30.0	34.0
2	31.808083028889097	31.0	31.0	34.0	30.0	34.0
3	31.822742623097053	31.0	31.0	34.0	30.0	34.0
4	35.586115672698966	37.0	35.0	37.0	33.0	37.0
5	35.40783129997198	37.0	35.0	37.0	33.0	37.0
6	35.39651047370698	37.0	35.0	37.0	33.0	37.0
7	35.256451093818335	37.0	35.0	37.0	32.0	37.0
8	35.235105220408194	37.0	35.0	37.0	32.0	37.0
9	36.748716009621184	39.0	35.0	39.0	32.0	39.0
10-11	36.66709312437397	39.0	35.0	39.0	32.0	39.0
12-13	36.59627231166602	38.0	35.0	39.0	32.0	39.0
14-15	37.65182650824171	39.0	36.5	41.0	32.0	41.0
16-17	37.68788340424261	39.0	37.0	41.0	32.0	41.0
18-19	37.70688017452052	39.0	37.0	41.0	32.0	41.0
20-21	37.677509328730494	39.0	37.0	41.0	32.0	41.0
22-23	37.605498015893374	39.0	36.5	41.0	32.0	41.0
24-25	37.50920524664289	39.0	36.0	41.0	32.0	41.0
26-27	37.337093682597846	39.0	36.0	40.5	31.5	41.0
28-29	37.18826407826045	39.0	36.0	40.0	31.0	41.0
30-31	37.04265604362791	39.0	36.0	40.0	31.0	41.0
32-33	37.28153548245086	39.0	36.0	40.0	31.0	41.0
34-35	37.288347045700235	39.0	36.0	41.0	31.0	41.0
36-37	37.17258552708612	39.0	36.0	40.5	31.0	41.0
38-39	37.05760740896067	39.0	36.0	40.0	31.0	41.0
40-41	37.03652268283739	39.0	35.5	40.0	31.0	41.0
42-43	36.96512235971745	39.0	35.0	40.0	30.5	41.0
44-45	36.935534060292746	39.0	35.0	40.0	30.5	41.0
46-47	36.950400983005956	39.0	35.0	40.0	30.5	41.0
48-49	36.87473578777663	39.0	35.0	40.0	30.0	41.0
50-51	36.79120658599453	39.0	35.0	40.0	30.0	41.0
52-53	36.663469674303094	39.0	35.0	40.0	30.0	41.0
54-55	36.55166455177814	38.0	35.0	40.0	30.0	41.0
56-57	36.38107363240431	38.0	35.0	40.0	30.0	41.0
58-59	36.23562888763503	38.0	34.5	40.0	29.0	41.0
60-61	36.073695753737205	38.0	34.0	40.0	29.0	41.0
62-63	35.843698251999385	38.0	34.0	40.0	29.0	41.0
64-65	35.62981670810311	37.0	34.0	40.0	29.0	41.0
66-67	35.37381451100905	37.0	34.0	39.5	28.0	41.0
68-69	35.099340932496744	36.0	33.5	39.0	28.0	40.5
70-71	34.81613938390303	36.0	33.0	39.0	28.0	40.0
72-73	34.51203821090618	35.5	33.0	38.5	28.0	40.0
74-75	34.190617881305656	35.0	33.0	37.5	28.0	40.0
76-77	33.151597774783795	34.5	31.5	36.0	26.0	39.0
78-79	33.52257503923401	35.0	32.0	36.5	27.0	39.0
80-81	33.45694217106298	35.0	32.0	36.0	28.0	38.5
82-83	33.26918898810008	35.0	32.0	36.0	27.5	37.5
84-85	33.04018688569998	34.5	32.0	35.5	27.0	37.0
86-87	32.87207361222935	34.0	32.0	35.0	27.0	37.0
88-89	32.6836301416032	34.0	32.0	35.0	27.0	36.0
90-91	32.5855801904852	34.0	32.0	35.0	27.0	36.0
92-93	32.54837306924831	34.0	32.0	35.0	27.0	36.0
94-95	32.536042443758774	34.0	32.0	35.0	27.0	35.0
96-97	32.44768280370553	34.0	32.0	35.0	27.0	35.0
98-99	32.59263779237483	34.0	32.0	35.0	28.0	35.0
100-101	32.14298457563244	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	24.0
20	130.0
21	723.0
22	3151.0
23	10295.0
24	25700.0
25	52384.0
26	91720.0
27	140896.0
28	196463.0
29	256561.0
30	322278.0
31	394808.0
32	483014.0
33	595311.0
34	752991.0
35	976547.0
36	1348359.0
37	1879292.0
38	1855181.0
39	773102.0
40	20270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.4843651821891	22.59204157810375	14.687936306218049	25.235656933489103
2	14.711889876541706	23.442889670920334	38.809663477488684	23.035556975049275
3	17.33416483253504	27.08117452012947	31.47248055938362	24.112180087951867
4	12.458145057314894	19.075794138716546	38.34303743200353	30.123023371965036
5	12.666177040955265	38.215768148472115	36.26786534585612	12.850189464716493
6	30.070091924673086	39.00615411578352	18.145298984085592	12.778454975457807
7	25.717276124930788	32.70995453082579	24.032949924178748	17.539819420064674
8	24.193325922144794	37.29743504501924	21.427677449042186	17.081561583793782
9	25.326243584468884	15.795871661477657	21.212061375329544	37.665823378723914
10-11	23.918780879133575	26.923048206912842	29.661376272643714	19.49679464130987
12-13	24.40265283942215	24.56779954988299	29.588223199013747	21.441324411681116
14-15	21.535077791937365	26.0868335087891	26.790243127065732	25.587845572207808
16-17	21.45126475508301	29.00083346399188	27.651174885580442	21.896726895344663
18-19	21.50558101061384	28.442140608851812	28.996149977011854	21.056128403522493
20-21	22.14669743635944	27.7058490334235	28.807991409060385	21.33946212115667
22-23	21.86029608717107	27.66350421896608	28.666413877850534	21.80978581601232
24-25	21.705032113732102	27.77439160280589	28.74015993742814	21.780416346033874
26-27	21.730859064384216	27.872966278433285	28.668560608761062	21.72761404842144
28-29	21.741906895256808	27.954231003090058	28.639520271437384	21.66434183021575
30-31	21.920767469902845	27.845647258381206	28.558627807039468	21.67495746467648
32-33	21.735227909890874	28.00422723282836	28.533416608384503	21.72712824889626
34-35	21.845625294205593	28.009096133003336	28.53637600575186	21.608902567039216
36-37	21.82291236705347	27.987326385357807	28.507467418652816	21.682293828935915
38-39	21.72725930941226	28.033982441394183	28.58863177775498	21.650126471438575
40-41	21.899683721340867	27.941134110868788	28.450874447694396	21.70830772009594
42-43	21.790978218051844	28.062123761257013	28.267125047364434	21.87977297332671
44-45	21.91661425772701	28.07727609215023	28.133479717456783	21.872629932665983
46-47	21.95675859537902	28.091376476341683	27.961785462514698	21.99007946576459
48-49	21.863203654816914	28.199374383126752	27.95635144273378	21.981070519322554
50-51	21.90752086677316	28.253939262501564	27.874892336812035	21.96364753391324
52-53	22.026521632312882	28.150339154159425	27.790235923166346	22.032903290361347
54-55	21.963671167342664	28.075711856802226	27.869949095175635	22.09066788067948
56-57	21.99442893622486	28.15152773600817	27.8372887232631	22.01675460450387
58-59	21.98065408416566	28.20395268991514	27.780108558772753	22.03528466714645
60-61	22.039167995853457	28.16191724363212	27.82885958651728	21.970055173997142
62-63	22.05693950625176	28.246544055343325	27.7477311352661	21.948785303138816
64-65	21.998827104806562	28.17068648943958	27.771031385151296	22.059455020602563
66-67	22.025988833743117	28.197803771089625	27.67570499093301	22.100502404234245
68-69	21.988234244481035	28.168778324246485	27.73431926589897	22.108668165373505
70-71	21.993151254578788	28.019968878105107	27.781383755142286	22.20549611217382
72-73	22.04581012349438	28.080594465343744	27.690815904173398	22.182779506988474
74-75	21.906451745732348	28.144926518849406	27.694503164429797	22.254118570988453
76-77	22.013036453156335	28.09312104686349	27.669850426675364	22.223992073304814
78-79	21.968313407126903	28.131713924771063	27.618122250031384	22.281850418070654
80-81	22.02834565801752	28.117527505603018	27.63596702635478	22.21815981002468
82-83	21.948914046994933	28.129509082115344	27.731845830337882	22.189731040551845
84-85	22.012202820063667	28.11935755294817	27.675490370705226	22.19294925628294
86-87	21.94051208251999	28.111160646519785	27.74791034882321	22.200416922137013
88-89	21.95442359529395	28.101384744652556	27.728252001449967	22.215939658603528
90-91	21.970268273953625	28.13030613558946	27.75328794397694	22.146137646479975
92-93	21.975096298768914	28.098913222683613	27.757799349076294	22.16819112947118
94-95	21.830432350568415	28.130933908673246	27.851122840271998	22.18751090048634
96-97	21.726307720480463	28.219656795080123	27.88702038281227	22.16701510162714
98-99	21.90355448707606	28.829060311723513	27.96048597889574	21.30689922230469
100-101	25.648338786137842	36.106171051066255	28.748195330061062	9.497294832734845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3895.0
1	3248.5
2	3353.0
3	4325.0
4	5431.0
5	7606.5
6	9509.0
7	11354.5
8	12767.5
9	12861.5
10	12101.5
11	11285.0
12	12107.5
13	15717.0
14	24341.0
15	35776.5
16	44141.5
17	47624.0
18	48749.0
19	49091.0
20	48760.0
21	49266.5
22	51916.0
23	58654.5
24	70262.0
25	86887.0
26	107260.5
27	128232.5
28	150843.0
29	175600.5
30	198510.0
31	220225.5
32	242716.0
33	264922.0
34	286622.5
35	307989.5
36	331233.0
37	351967.5
38	368888.0
39	384381.0
40	398563.5
41	411767.5
42	420331.5
43	427580.0
44	434232.0
45	437588.5
46	437704.0
47	435613.5
48	432492.5
49	425122.0
50	414743.0
51	400440.5
52	380843.0
53	359712.0
54	337529.0
55	312521.0
56	286441.0
57	259800.0
58	230647.5
59	198616.5
60	165754.5
61	135557.5
62	108823.0
63	85068.0
64	64316.5
65	48541.0
66	36803.5
67	28053.5
68	21548.0
69	15787.0
70	11292.0
71	8080.5
72	5676.0
73	3912.5
74	2624.5
75	1813.5
76	1289.0
77	889.0
78	619.5
79	432.5
80	301.0
81	213.0
82	151.0
83	97.5
84	65.5
85	49.5
86	41.5
87	35.5
88	27.5
89	19.5
90	12.5
91	7.5
92	5.0
93	4.0
94	4.0
95	3.5
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002957009212115211
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.171484135694697E-4
14-15	0.0
16-17	0.0
18-19	4.3225383831584476E-4
20-21	0.0
22-23	0.0015180854829513844
24-25	1.76911835839183E-4
26-27	0.004960694973208314
28-29	3.9357990491207894E-4
30-31	0.0
32-33	0.0
34-35	2.4785582405171104E-4
36-37	0.020359819057279075
38-39	0.008722446334358033
40-41	0.013499388807199674
42-43	0.001832522735532769
44-45	0.0030160654204696804
46-47	5.215349608353321E-6
48-49	8.175242601642793E-4
50-51	1.5993538610401397E-5
52-53	1.0770729723401198E-5
54-55	0.004441313028477471
56-57	0.009091348615389258
58-59	0.01159371821133453
60-61	0.006320551034668561
62-63	0.006974439962752721
64-65	7.978815204915922E-4
66-67	8.201172416176695E-5
68-69	0.0
70-71	1.2053391703891558E-4
72-73	0.0
74-75	1.178256942491449E-4
76-77	3.1467117208655246E-5
78-79	6.391930826524596E-6
80-81	0.0020795420952283415
82-83	0.002081548047944065
84-85	0.008968991877250981
86-87	0.010284962482780376
88-89	0.04247108923829518
90-91	4.7589627961335624E-4
92-93	5.807766275738659E-4
94-95	0.0
96-97	0.009266531764988173
98-99	5.6471619340626075E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	371.0
20-21	1087.0
22-23	2148.0
24-25	4296.0
26-27	6032.0
28-29	10555.0
30-31	23408.0
32-33	33815.0
34-35	48936.0
36-37	71715.0
38-39	78835.0
40-41	81050.0
42-43	92463.0
44-45	109458.0
46-47	109218.0
48-49	102926.0
50-51	95044.0
52-53	95789.0
54-55	104853.0
56-57	109285.0
58-59	110354.0
60-61	108441.0
62-63	104304.0
64-65	110313.0
66-67	117036.0
68-69	120836.0
70-71	119825.0
72-73	115156.0
74-75	117571.0
76-77	119911.0
78-79	128146.0
80-81	128171.0
82-83	124527.0
84-85	124380.0
86-87	131919.0
88-89	140767.0
90-91	150202.0
92-93	159292.0
94-95	193968.0
96-97	249582.0
98-99	810416.0
100-101	5512803.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.26128380888707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.21601228174274	79.59626335632083
2	6.2037338941846585	10.826915680305193
3	1.4908470579755917	3.9027968472495727
4	0.5065366151755705	1.7680414134571387
5	0.22727970568096117	0.9916359450713336
6	0.11780188395159095	0.6167726177232814
7	0.07105324759616086	0.43401383228222
8	0.047763001488383215	0.3334288662753683
9	0.02703388013826806	0.21231099784807603
>10	0.09051338681843171	1.2130766553396188
>50	0.0012369663915048669	0.0703386953127737
>100	1.710267669647658E-4	0.02623604308379146
>500	1.7052089260736093E-5	0.008169049730830465
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.823950870814654E-6	0.0	0.0	0.0
3	0.0	9.823950870814654E-6	0.0	0.0	0.0
4	0.0	2.947185261244396E-5	0.0	0.0	0.0
5	0.0	2.947185261244396E-5	0.0	0.0	0.0
6	9.823950870814654E-5	2.947185261244396E-5	0.0	0.0	0.0
7	9.823950870814654E-5	2.947185261244396E-5	0.0	0.0	0.0
8	9.823950870814654E-5	3.9295803483258614E-5	0.0	0.0	0.0
9	9.823950870814654E-5	1.0806345957896119E-4	0.0	9.823950870814654E-6	9.823950870814654E-6
10-11	1.0806345957896119E-4	1.1788741044977584E-4	0.0	9.823950870814654E-6	9.823950870814654E-6
12-13	1.1788741044977584E-4	1.1788741044977584E-4	0.0	1.473592630622198E-5	9.823950870814654E-6
14-15	1.277113613205905E-4	1.670071648038491E-4	0.0	1.9647901741629307E-5	2.4559877177036634E-5
16-17	2.3086284546414436E-4	1.7683111567466376E-4	0.0	1.9647901741629307E-5	2.947185261244396E-5
18-19	2.3577482089955169E-4	2.013909928517004E-4	0.0	1.9647901741629307E-5	2.947185261244396E-5
20-21	2.3577482089955169E-4	2.210388945933297E-4	0.0	2.4559877177036634E-5	3.438382804785129E-5
22-23	2.603346980765883E-4	2.4559877177036634E-4	0.0	5.4031729789480595E-5	4.911975435407327E-5
24-25	2.947185261244396E-4	2.70158648947403E-4	0.0	1.277113613205905E-4	6.385568066029525E-5
26-27	3.2419037873688357E-4	2.8489457525362495E-4	0.0	2.0630296828710773E-4	6.876765609570258E-5
28-29	3.2419037873688357E-4	2.9963050155984693E-4	0.0	5.55053224201028E-4	6.876765609570258E-5
30-31	3.438382804785129E-4	3.291023541722909E-4	0.0	0.0017584872058758229	6.876765609570258E-5
32-33	3.5366223134932753E-4	3.487502559139202E-4	0.0	0.0043372743094646695	6.876765609570258E-5
34-35	3.7822210852636416E-4	4.2734186288043743E-4	0.0	0.008060551689503422	6.876765609570258E-5
36-37	3.9295803483258614E-4	4.911975435407327E-4	0.0	0.013807562948929996	7.36796315311099E-5
38-39	3.9295803483258614E-4	5.010214944115473E-4	0.0	0.02209897748389756	9.332753327273921E-5
40-41	3.9295803483258614E-4	5.010214944115473E-4	0.0	0.030670374618683348	9.823950870814654E-5
42-43	3.9295803483258614E-4	5.40317297894806E-4	0.0	0.03843620778206233	9.823950870814654E-5
44-45	4.076939611388081E-4	6.041729785551011E-4	0.0	0.04784755271630277	9.823950870814654E-5
46-47	4.371658137512521E-4	6.483807574737671E-4	0.0	0.058103757425433264	1.0806345957896119E-4
48-49	4.420777891866594E-4	6.876765609570258E-4	0.0	0.06730879939138659	1.1788741044977584E-4
50-51	4.8628556810532535E-4	7.859160696651723E-4	0.0	0.07643524975037341	1.1788741044977584E-4
52-53	5.304933470239913E-4	7.859160696651723E-4	0.0	0.08509506244299653	1.1788741044977584E-4
54-55	5.40317297894806E-4	7.908280451005797E-4	0.0	0.09484533368228007	1.1788741044977584E-4
56-57	5.40317297894806E-4	7.957400205359869E-4	0.0	0.10456122109351576	1.277113613205905E-4
58-59	5.501412487656206E-4	8.202998977130235E-4	0.0	0.11396765405232079	1.3753531219140515E-4
60-61	5.648771750718425E-4	8.301238485838383E-4	0.0	0.1224899314327525	1.473592630622198E-4
62-63	5.992610031196939E-4	8.595957011962821E-4	0.0	0.13023611669438984	1.473592630622198E-4
64-65	6.041729785551011E-4	9.725711362106507E-4	0.0	0.13847349949956794	1.5227123849762713E-4
66-67	6.090849539905085E-4	9.725711362106507E-4	0.0	0.14609197339988472	1.5718321393303446E-4
68-69	6.090849539905085E-4	9.823950870814654E-4	0.0	0.1539314861947948	1.5718321393303446E-4
70-71	6.139969294259159E-4	9.823950870814654E-4	0.0	0.16120612181463306	1.7683111567466376E-4
72-73	6.582047083445818E-4	9.922190379522801E-4	0.0	0.16756713000348555	2.1612691915792238E-4
74-75	6.582047083445818E-4	0.0010462507677417607	0.0	0.17334852509095997	2.1612691915792238E-4
76-77	6.680286592153964E-4	0.0010953705220958338	0.0	0.17815734904222374	2.1612691915792238E-4
78-79	6.680286592153964E-4	0.0011150184238374631	0.0	0.1822342886536118	2.1612691915792238E-4
80-81	6.778526100862111E-4	0.0011199303992728705	0.0	0.184901491315038	2.1612691915792238E-4
82-83	6.778526100862111E-4	0.001129754350143685	0.0	0.18675821802962195	2.1612691915792238E-4
84-85	6.778526100862111E-4	0.0011395783010144998	0.0	0.18847249745657912	2.1612691915792238E-4
86-87	6.778526100862111E-4	0.0011592262027561291	0.0	0.18870336030204327	2.1612691915792238E-4
88-89	6.778526100862111E-4	0.0012132579325456097	0.0	0.18875739203183275	2.1612691915792238E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9385	0.0	44.181793	1
GTATCAA	16125	0.0	30.38053	1
TCAACGC	18985	0.0	24.845608	4
ATCAACG	19360	0.0	24.407059	3
CAACGCA	19780	0.0	23.86791	5
AACGCAG	20555	0.0	22.988111	6
TATCAAC	21015	0.0	22.95716	2
GTACATG	23545	0.0	20.560558	1
TACATGG	23580	0.0	20.021608	2
ACGCAGA	24375	0.0	19.436726	7
ACATGGG	23805	0.0	19.259186	3
CGCAGAG	24755	0.0	19.138365	8
AGAGTAC	26335	0.0	16.436012	10-11
CATGGGG	17825	0.0	16.397001	4
GAGTACT	17505	0.0	15.540081	12-13
GCAGAGT	30455	0.0	15.230612	9
CAGAGTA	29425	0.0	14.7240715	10-11
GTGGTAT	5440	0.0	13.982848	1
AGTACTT	19860	0.0	13.863871	12-13
GAGTACA	17320	0.0	13.819979	1
>>END_MODULE