FastQCFastQC Report
Fri 27 May 2016
SRR2139833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14155929
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164800.052.0626641
GTATCAA301800.035.0576061
TCAACGC374000.027.6218094
ATCAACG376850.027.4349863
CAACGCA385200.026.7862935
AACGCAG394500.026.2075376
TATCAAC398500.026.0391392
ACGCAGA461600.022.4249367
CGCAGAG467050.022.181078
GCAGAGT548250.018.7517579
GTACATG403550.018.4063851
TACATGG399750.018.0403522
GAGTACT326150.018.03678912-13
AGAGTAC503850.018.0022410-11
ACATGGG400100.017.5043353
CAGAGTA545550.017.17126710-11
GTGGTAT79750.016.9487271
TGGTATC74100.016.0521132
AGTACTT361250.015.95042712-13
GTACTTT374100.015.70939914-15
Produced by FastQC (version 0.11.5)