FastQCFastQC Report
Fri 27 May 2016
SRR2139832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12623999
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140400.048.3262331
GTATCAA245100.033.779251
TCAACGC297500.027.0124574
ATCAACG302150.026.6521513
CAACGCA305350.026.304315
AACGCAG313600.025.6790436
TATCAAC323400.025.2772182
ACGCAGA363850.022.155617
CGCAGAG367050.021.9510528
GTACATG315500.018.759321
TACATGG312300.018.4020232
AGAGTAC397400.017.88379510-11
GCAGAGT448100.017.8685749
GAGTACT266150.017.62132812-13
ACATGGG320800.017.4701253
CAGAGTA435250.016.73248110-11
AGTACTT295800.015.84087612-13
CATGGGG225700.015.7443944
GTACTTT301550.015.58835714-15
GTGGTAT74800.014.0455641
Produced by FastQC (version 0.11.5)