##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139832_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12623999
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.112438934762274	33.0	31.0	34.0	30.0	34.0
2	32.263322501847476	34.0	31.0	34.0	30.0	34.0
3	32.31469924862954	34.0	31.0	34.0	30.0	34.0
4	35.9549368627168	37.0	35.0	37.0	35.0	37.0
5	35.84352398950602	37.0	35.0	37.0	35.0	37.0
6	35.86153983377217	37.0	35.0	37.0	35.0	37.0
7	35.78540849060587	37.0	35.0	37.0	33.0	37.0
8	35.76843296644748	37.0	35.0	37.0	33.0	37.0
9	37.41570773254973	39.0	37.0	39.0	34.0	39.0
10-11	37.32991863354869	39.0	37.0	39.0	34.0	39.0
12-13	37.31291439424227	39.0	37.0	39.0	34.0	39.0
14-15	38.54865324371461	40.0	38.0	41.0	34.0	41.0
16-17	38.53175534947365	40.0	38.0	41.0	34.0	41.0
18-19	38.56884137902736	40.0	38.0	41.0	34.0	41.0
20-21	38.54198041190348	40.0	38.0	41.0	34.0	41.0
22-23	38.47770937973524	40.0	38.0	41.0	34.0	41.0
24-25	38.3956742381356	40.0	38.0	41.0	34.0	41.0
26-27	38.263059521402326	40.0	38.0	41.0	34.0	41.0
28-29	38.170743650514886	40.0	38.0	41.0	33.0	41.0
30-31	38.06076088548846	40.0	37.0	41.0	33.0	41.0
32-33	38.220980024895624	40.0	38.0	41.0	34.0	41.0
34-35	38.20957922285078	40.0	38.0	41.0	34.0	41.0
36-37	38.12974762966842	40.0	37.5	41.0	33.0	41.0
38-39	38.00598775409501	40.0	37.0	41.0	33.0	41.0
40-41	37.95623714486578	40.0	37.0	41.0	33.0	41.0
42-43	37.8820756008286	40.0	37.0	41.0	33.0	41.0
44-45	37.879466517640104	40.0	37.0	41.0	33.0	41.0
46-47	37.91597695639457	40.0	37.0	41.0	33.0	41.0
48-49	37.84391639497138	40.0	37.0	41.0	33.0	41.0
50-51	37.78150033409257	40.0	37.0	41.0	33.0	41.0
52-53	37.64715820550686	40.0	36.5	41.0	32.0	41.0
54-55	37.52207618535464	39.5	36.0	41.0	32.0	41.0
56-57	37.3560096051205	39.0	36.0	41.0	32.0	41.0
58-59	37.19451501123166	39.0	35.5	41.0	31.0	41.0
60-61	37.04186020004255	39.0	35.0	40.0	31.0	41.0
62-63	36.82096619426217	38.5	35.0	40.0	31.0	41.0
64-65	36.60205446117705	38.0	35.0	40.0	31.0	41.0
66-67	36.34532199486057	37.5	35.0	40.0	31.0	41.0
68-69	36.05698418284568	37.0	34.5	40.0	30.5	41.0
70-71	35.7721854613643	37.0	34.0	39.0	30.0	41.0
72-73	35.45188134670708	36.0	34.0	39.0	30.0	40.5
74-75	35.11126299759628	36.0	34.0	38.5	30.0	40.0
76-77	34.02118561238623	35.0	32.5	37.0	28.5	39.0
78-79	34.36765728675746	35.0	33.5	37.0	29.0	39.0
80-81	34.25353189953295	35.0	34.0	37.0	29.0	39.0
82-83	33.99858111082115	35.0	33.0	36.0	29.0	38.5
84-85	33.781461889715885	35.0	33.0	36.0	29.0	37.0
86-87	33.592896783121475	35.0	33.0	35.5	29.0	37.0
88-89	33.382320397291295	35.0	33.0	35.0	29.0	36.5
90-91	33.26004181596528	35.0	33.0	35.0	29.0	36.0
92-93	33.19015971550895	35.0	33.0	35.0	29.0	36.0
94-95	33.150801481333374	35.0	33.0	35.0	29.0	36.0
96-97	33.098059673101496	34.0	33.0	35.0	29.0	35.0
98-99	33.17088482795404	34.0	33.0	35.0	29.0	35.0
100-101	32.62571727853785	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	14.0
20	72.0
21	362.0
22	1643.0
23	5853.0
24	15674.0
25	33972.0
26	61944.0
27	100506.0
28	146001.0
29	200429.0
30	259866.0
31	331520.0
32	422211.0
33	544302.0
34	728889.0
35	1009164.0
36	1534948.0
37	2549218.0
38	3084774.0
39	1528999.0
40	63636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.37915386400142	22.07474826320883	15.295723645098514	24.25037422769124
2	13.986297617348217	22.95883438964382	40.735689901483724	22.319178091524236
3	16.931069148532092	27.67808362468977	31.733312082803554	23.657535143974584
4	12.2272268874546	19.71788020578899	39.52460705993402	28.53028584682239
5	12.652234842540782	38.34472737204748	36.33551460198943	12.667523183422306
6	28.501142942105744	39.60112797854309	19.259721107392355	12.638007971958807
7	24.971453182149332	33.14705585765652	24.683454109906062	17.19803685028809
8	23.617722086321457	37.30270415895946	22.008754911973615	17.07081884274547
9	25.136836591954737	16.607391999951837	21.857487472868144	36.39828393522528
10-11	23.40993531447523	27.211840717034274	30.006391001773686	19.371832966716806
12-13	24.374562292028067	24.890725989442807	29.816368014604567	20.918343703924563
14-15	21.620732067548484	26.242829233430705	27.27561607062865	24.86082262839216
16-17	21.27132218562438	29.177739161734724	27.95267569333616	21.598262959304733
18-19	21.305775721647358	28.483802467520213	29.475142010066758	20.735279800765674
20-21	22.043359673413164	27.714374200666754	29.26784490864455	20.974421217275534
22-23	21.79915353515595	27.639428999682263	29.10040302964077	21.461014435521022
24-25	21.588845977457797	27.826393930899503	29.10239873689174	21.48236135475097
26-27	21.595766713405904	27.93447918782732	29.019266805067655	21.450487293699123
28-29	21.55873681726218	27.98098158302611	29.03510099231764	21.425180607394072
30-31	21.81087633138747	27.83515562846245	28.947656671109605	21.40631136904048
32-33	21.6008594519716	27.9979391748508	28.898009812721483	21.50319156045612
34-35	21.719809188351512	28.00254525238176	28.89260472055566	21.385040838711067
36-37	21.703403541428195	27.973448444581372	28.844593550484642	21.478554463505787
38-39	21.543101806816974	28.062935508539056	28.93593433097848	21.45802835366549
40-41	21.76595483200177	27.937277607695698	28.788356280152488	21.508411280150046
42-43	21.66850644170474	28.06364444044247	28.572690859133314	21.695158258719474
44-45	21.756218548868933	28.121336802384068	28.43672126588827	21.68572338285873
46-47	21.847867141439153	28.09482335389054	28.193570941877	21.8637385627933
48-49	21.76067065091566	28.186296104430888	28.19516607147718	21.85786717317627
50-51	21.79186608311024	28.282711530603972	28.068278140389037	21.857144245896755
52-53	21.94985888717357	28.16688770445996	27.965267699910846	21.917985708455625
54-55	21.887843908510632	28.050578370105328	28.051642348012606	22.009935373371434
56-57	21.923324937342734	28.128522289948048	28.011713549650935	21.93643922305828
58-59	21.896405249476956	28.16383760390007	27.99955509136755	21.94020205525543
60-61	21.924030406973987	28.140268449072924	28.013010671182037	21.922690472771052
62-63	21.88340033685183	28.231393380251703	27.993358749745877	21.89184753315059
64-65	21.857924877462896	28.193781962595004	27.950960267590645	21.99733289235145
66-67	21.941769031428382	28.230971358801572	27.79236146138017	22.034898148389875
68-69	21.9074379757993	28.24893160152412	27.80619953739961	22.037430885276965
70-71	21.90062273185575	28.042030161853898	27.895881237676466	22.161465868613885
72-73	21.927135310665058	28.036082972608895	27.84254194333325	22.1942397733928
74-75	21.851678185721415	28.124388704939303	27.78516098402791	22.238772125311367
76-77	22.047834744071956	28.043702159198958	27.726188315929633	22.182274780799453
78-79	22.011371194357213	28.054648112983205	27.68702739650937	22.246953296150206
80-81	21.984512036349145	28.048294296820604	27.70514274528254	22.26205092154771
82-83	22.037401865061355	27.99619740968513	27.758692761879978	22.207707963373544
84-85	22.098229587216373	28.00397688350188	27.747128647339338	22.150664881942415
86-87	22.007017827565516	28.014790524951266	27.796367446307972	22.18182420117525
88-89	22.04352307307673	28.024604752909493	27.765715778454116	22.16615639555966
90-91	22.073352196612696	27.990450286724844	27.836092445047285	22.10010507161518
92-93	22.12169468423951	27.887133702853234	27.85156903095561	22.13960258195165
94-95	21.992142445828783	27.8882333718175	27.905705479901897	22.21391870245182
96-97	21.940184021786738	27.907306593659907	28.007250577186717	22.145258807366634
98-99	22.147634251801104	28.479838140308722	28.106833339087416	21.265694268802754
100-101	25.916616131130688	35.63089845069693	28.960692342314665	9.491793075857709
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3056.0
1	2714.0
2	3454.5
3	4790.0
4	6373.5
5	9844.5
6	12805.5
7	15240.0
8	16903.0
9	16825.0
10	15703.5
11	14704.0
12	16321.0
13	21467.0
14	33403.0
15	49688.5
16	62555.0
17	69002.5
18	70313.0
19	68968.0
20	66304.0
21	64595.5
22	66657.5
23	75376.0
24	91586.5
25	114292.0
26	142157.0
27	170381.0
28	198511.5
29	227471.0
30	254120.0
31	280877.0
32	309762.0
33	338928.5
34	366331.5
35	390828.5
36	419335.5
37	446122.0
38	465183.0
39	480606.0
40	494284.5
41	507314.5
42	514675.5
43	518889.0
44	524140.0
45	528100.0
46	527303.0
47	523034.5
48	516822.0
49	505800.0
50	492309.5
51	475199.5
52	450570.0
53	421971.0
54	393645.5
55	363789.5
56	332072.0
57	299416.0
58	264478.0
59	227232.5
60	189793.5
61	154967.5
62	123555.0
63	95621.5
64	71631.0
65	53492.0
66	40038.5
67	30284.0
68	23121.5
69	16887.5
70	11871.5
71	8450.5
72	6012.5
73	4181.0
74	2867.0
75	1913.0
76	1221.0
77	766.0
78	510.0
79	351.0
80	250.0
81	166.5
82	119.5
83	86.0
84	61.5
85	49.5
86	42.5
87	34.5
88	24.0
89	15.0
90	7.0
91	3.0
92	3.5
93	4.0
94	3.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003968631493079174
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.54499410210663E-4
20-21	0.001417980090529711
22-23	0.002618370343727458
24-25	3.16965587006598E-5
26-27	0.008021989446248488
28-29	1.5067776046217153E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03468052945795645
38-39	0.015281362063911518
40-41	0.02445095944961958
42-43	0.0013144989729655212
44-45	0.00557459378880751
46-47	0.004517111169419267
48-49	0.0025651015349864373
50-51	0.002372449269090776
52-53	1.7287117894729836E-4
54-55	0.002594468975863241
56-57	0.014292440324988782
58-59	0.009947167461765353
60-61	0.0034621262275035987
62-63	0.0010814475402521136
64-65	0.0031777606715471625
66-67	2.4155538628097334E-4
68-69	5.5489918610397E-4
70-71	2.571612502951402E-4
72-73	0.0
74-75	0.0
76-77	0.006229639338491408
78-79	2.9562292183975465E-4
80-81	1.524575702958988E-5
82-83	9.795697327594567E-5
84-85	0.0019869381821178388
86-87	0.005395425824831691
88-89	0.08068732191698076
90-91	0.0030248136643546207
92-93	0.0015011381026406806
94-95	0.0
96-97	0.017389445767271233
98-99	2.4355912461684342E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	217.0
20-21	1002.0
22-23	1978.0
24-25	4221.0
26-27	5309.0
28-29	10142.0
30-31	30234.0
32-33	40143.0
34-35	56465.0
36-37	85897.0
38-39	91516.0
40-41	98565.0
42-43	111801.0
44-45	131496.0
46-47	131672.0
48-49	119498.0
50-51	109989.0
52-53	100286.0
54-55	109424.0
56-57	115175.0
58-59	119129.0
60-61	118291.0
62-63	113543.0
64-65	122121.0
66-67	130339.0
68-69	132954.0
70-71	132132.0
72-73	126633.0
74-75	128766.0
76-77	131612.0
78-79	139390.0
80-81	141463.0
82-83	136506.0
84-85	136113.0
86-87	143752.0
88-89	151750.0
90-91	163680.0
92-93	168333.0
94-95	206478.0
96-97	264807.0
98-99	1013588.0
100-101	7347589.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.28789075005325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.12193099679868	76.86309405038804
2	6.922550559943384	11.808194717363426
3	1.6151969097158123	4.132702127269974
4	0.5925049680288419	2.0213399592843064
5	0.27413674947745353	1.169027257000389
6	0.1544498272841396	0.7903619997464577
7	0.08942069356818728	0.5338551640686283
8	0.05861315773802109	0.39991940749407845
9	0.03646562141569	0.27990683418907536
>10	0.1315904807534429	1.7639542298809363
>50	0.002602124193174169	0.1457870355590792
>100	5.06809226924276E-4	0.07151174307363617
>500	3.110185627238705E-5	0.020345474681992787
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.752852087519969E-5	0.0	0.0	0.0
3	0.0	4.752852087519969E-5	0.0	7.921420145866615E-6	0.0
4	0.0	1.1089988204213261E-4	0.0	7.921420145866615E-6	0.0
5	0.0	1.1089988204213261E-4	0.0	1.584284029173323E-5	0.0
6	1.584284029173323E-5	1.1882130218799923E-4	0.0	1.584284029173323E-5	0.0
7	1.584284029173323E-5	1.1882130218799923E-4	0.0	1.584284029173323E-5	0.0
8	1.584284029173323E-5	1.2674272233386584E-4	0.0	1.584284029173323E-5	0.0
9	1.584284029173323E-5	2.2972118423013183E-4	0.0	1.584284029173323E-5	0.0
10-11	1.584284029173323E-5	2.2972118423013183E-4	0.0	3.168568058346646E-5	7.921420145866615E-6
12-13	1.584284029173323E-5	2.2972118423013183E-4	0.0	3.564639065639977E-5	7.921420145866615E-6
14-15	1.584284029173323E-5	3.08935385688798E-4	0.0	4.752852087519969E-5	7.921420145866615E-6
16-17	4.356781080226638E-5	3.08935385688798E-4	0.0	7.525349138573284E-5	7.921420145866615E-6
18-19	4.752852087519969E-5	3.2081751590759793E-4	0.0	1.0693917196919931E-4	7.921420145866615E-6
20-21	5.5449941021066304E-5	3.4854248641813106E-4	0.0	2.0595692379253198E-4	7.921420145866615E-6
22-23	5.941065109399961E-5	3.881495871474641E-4	0.0	5.901458008670628E-4	7.921420145866615E-6
24-25	7.129278131279954E-5	4.554816583873303E-4	0.0	0.0014892269874229236	1.584284029173323E-5
26-27	8.317491153159946E-5	4.832066288978635E-4	0.0	0.0041389420262153065	1.584284029173323E-5
28-29	8.713562160453276E-5	4.911280490437301E-4	0.0	0.02271467226827252	1.584284029173323E-5
30-31	9.505704175039937E-5	5.465779900647964E-4	0.0	0.03359870354869325	1.584284029173323E-5
32-33	1.0297846189626599E-4	5.54499410210663E-4	0.0	0.04105275990595373	1.584284029173323E-5
34-35	1.0297846189626599E-4	6.257921915234626E-4	0.0	0.051089199230766734	1.584284029173323E-5
36-37	1.0297846189626599E-4	6.970849728362621E-4	0.0	0.06459125986939637	1.584284029173323E-5
38-39	1.0297846189626599E-4	7.20849233273862E-4	0.0	0.08841889166816316	1.584284029173323E-5
40-41	1.0297846189626599E-4	7.683777541490617E-4	0.0	0.13283033371596434	1.584284029173323E-5
42-43	1.0297846189626599E-4	8.000634347325281E-4	0.0	0.15266556976121434	1.584284029173323E-5
44-45	1.1089988204213261E-4	9.426489973581272E-4	0.0	0.1803667760113099	1.584284029173323E-5
46-47	1.1882130218799923E-4	0.0010456274592543931	0.0	0.21227821706893354	1.584284029173323E-5
48-49	1.2278201226093255E-4	0.0010495881693273264	0.0	0.23875952461656563	1.584284029173323E-5
50-51	1.3862485255266576E-4	0.0011010774002754595	0.0	0.263026795233428	1.584284029173323E-5
52-53	1.4654627269853238E-4	0.0011010774002754595	0.0	0.29590068883877446	1.584284029173323E-5
54-55	1.5050698277146567E-4	0.0011129595304942594	0.0	0.3641595662357071	1.584284029173323E-5
56-57	1.5050698277146567E-4	0.0011248416607130593	0.0	0.43546422967872545	1.584284029173323E-5
58-59	1.5050698277146567E-4	0.0011367237909318591	0.0	0.4927559008837057	1.584284029173323E-5
60-61	1.5446769284439897E-4	0.0011604880513694591	0.0	0.528065631183906	1.584284029173323E-5
62-63	1.663498230631989E-4	0.001172370181588259	0.0	0.5484791308998044	1.584284029173323E-5
64-65	1.663498230631989E-4	0.0013822878154537243	0.0	0.562698080061635	1.584284029173323E-5
66-67	1.663498230631989E-4	0.001390209235599591	0.0	0.5711027068363994	1.584284029173323E-5
68-69	1.663498230631989E-4	0.001429816336328924	0.0	0.5790756162132142	1.584284029173323E-5
70-71	1.663498230631989E-4	0.0014496198866935906	0.0	0.5865930439316416	1.584284029173323E-5
72-73	1.663498230631989E-4	0.0014575413068394572	0.0	0.5925222269108228	1.584284029173323E-5
74-75	1.663498230631989E-4	0.0014892269874229236	0.0	0.5977543249171677	1.584284029173323E-5
76-77	1.663498230631989E-4	0.00154467692844399	0.0	0.6026220375968028	1.584284029173323E-5
78-79	1.663498230631989E-4	0.0015842840291733229	0.0	0.6070382293281233	1.584284029173323E-5
80-81	1.663498230631989E-4	0.0015882447392462562	0.0	0.6095810051949465	1.584284029173323E-5
82-83	1.663498230631989E-4	0.0016001268694650563	0.0	0.61139501040835	1.584284029173323E-5
84-85	1.663498230631989E-4	0.0016001268694650563	0.0	0.6129951372778151	1.584284029173323E-5
86-87	1.703105331361322E-4	0.0016080482896109229	0.0	0.613351601184379	1.584284029173323E-5
88-89	1.7427124320906553E-4	0.0017031053313613222	0.0	0.6133872475750355	1.584284029173323E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14040	0.0	48.326233	1
GTATCAA	24510	0.0	33.77925	1
TCAACGC	29750	0.0	27.012457	4
ATCAACG	30215	0.0	26.652151	3
CAACGCA	30535	0.0	26.30431	5
AACGCAG	31360	0.0	25.679043	6
TATCAAC	32340	0.0	25.277218	2
ACGCAGA	36385	0.0	22.15561	7
CGCAGAG	36705	0.0	21.951052	8
GTACATG	31550	0.0	18.75932	1
TACATGG	31230	0.0	18.402023	2
AGAGTAC	39740	0.0	17.883795	10-11
GCAGAGT	44810	0.0	17.868574	9
GAGTACT	26615	0.0	17.621328	12-13
ACATGGG	32080	0.0	17.470125	3
CAGAGTA	43525	0.0	16.732481	10-11
AGTACTT	29580	0.0	15.840876	12-13
CATGGGG	22570	0.0	15.744394	4
GTACTTT	30155	0.0	15.588357	14-15
GTGGTAT	7480	0.0	14.045564	1
>>END_MODULE