FastQCFastQC Report
Fri 27 May 2016
SRR2139831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12132735
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130000.047.3553121
GTATCAA231250.033.5794261
TCAACGC278400.027.1254394
ATCAACG281550.026.8071143
CAACGCA289050.026.1838425
AACGCAG295400.025.7200156
TATCAAC302150.025.2435042
ACCGAGG135650.023.48542492-93
CGAGACC130500.023.27303588-89
GACCGAG134800.023.26172690-91
AGACCGA133100.023.1626990-91
GAGACCG133450.023.05325188-89
ACGAGAC136950.022.66576286-87
ACGCAGA343500.022.167137
CGCAGAG350200.021.6952958
GCCCACG139750.021.51933582-83
CACGAGA142950.021.23740886-87
CCCACGA142150.021.04885384-85
CCACGAG146450.020.8084484-85
ATCTCCG139900.020.77133674-75
Produced by FastQC (version 0.11.5)