FastQCFastQC Report
Fri 27 May 2016
SRR2139830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12275066
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127800.046.392231
TATCTCG156550.036.5292994-95
GTATCAA222350.034.9004171
CCGTCTT62850.032.42830792-93
TATGCCG63900.030.46498588-89
TGCCGTC66850.029.48934790-91
GCCGTCT65000.029.44755490-91
TCAACGC269800.028.0330834
ATCAACG274850.027.6111873
ATGCCGT68100.027.54973688-89
CAACGCA278300.027.2382265
AACGCAG286100.026.5553026
CGTATGC73450.026.44187286-87
TATCAAC289300.026.4106122
CTCGTAT76150.025.34830384-85
TCGTATG73700.025.30638584-85
GCTATCT247450.024.81999694-95
CTATCTC245500.024.8070994-95
TCTCGTA78000.023.84565282-83
ATCTCGT124350.022.80749994-95
Produced by FastQC (version 0.11.5)